Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,985,345 A→G K2233E (AAA→GAA)  pvdD → pyoverdine non‑ribosomal peptide synthetase PvdD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,985,3450AG100.0% 1756.6 / NA 448K2233E (AAA→GAA) pvdDpyoverdine non‑ribosomal peptide synthetase PvdD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (214/234);  total (214/234)

Only 100 of 452 total aligned reads displayed.

TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAAC  >  NC_008463/2985198‑2985491
                                                                                                                                                   |                                                                                                                                                  
tGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCgaa                                                                                                                                                  >  1:8887617/1‑150 (MQ=255)
 gTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAg                                                                                                                                                 <  2:7704584/150‑1 (MQ=255)
      aaGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtt                                                                                                                                            <  1:2318599/150‑1 (MQ=255)
        gACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtttt                                                                                                                                          >  1:1262527/1‑150 (MQ=255)
         aCCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTc                                                                                                                                         >  1:6031494/1‑150 (MQ=255)
          cccTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCa                                                                                                                                        <  1:7889730/150‑1 (MQ=255)
          cccTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAAAACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCa                                                                                                                                        <  2:7228631/150‑1 (MQ=255)
           ccTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCaa                                                                                                                                       <  2:4106209/150‑1 (MQ=255)
            cTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAc                                                                                                                                      >  1:2537608/1‑150 (MQ=255)
             tCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAcc                                                                                                                                     <  1:8088065/150‑1 (MQ=255)
              cGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCt                                                                                                                                    <  2:8322892/150‑1 (MQ=255)
                ccATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTgg                                                                                                                                  <  2:7346501/150‑1 (MQ=255)
                  aTGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAcc                                                                                                                                     >  2:5443511/1‑145 (MQ=255)
                  aTGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCg                                                                                                                                >  1:9502656/1‑150 (MQ=255)
                    ggCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCggg                                                                                                                              <  2:6203167/150‑1 (MQ=255)
                     gcgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGa                                                                                                                             >  2:3414529/1‑150 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  1:2144994/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  2:8796226/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  1:6845117/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  1:10907049/150‑1 (MQ=255)
                          gCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGagtgtc                                                                                                                        >  1:10468881/1‑145 (MQ=255)
                          gCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTc                                                                                                                        <  2:1615762/150‑1 (MQ=255)
                          gCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTc                                                                                                                        >  1:7311908/1‑150 (MQ=255)
                             ggATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCt                                                                                                                     <  1:10018543/150‑1 (MQ=255)
                             ggATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCt                                                                                                                     <  2:3563301/150‑1 (MQ=255)
                              gATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTc                                                                                                                    >  2:4446091/1‑150 (MQ=255)
                               aTCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcg                                                                                                                   >  1:1718401/1‑150 (MQ=255)
                               aTCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGATTTCAACCTGGCGGGATGGTCGCTcg                                                                                                                   >  1:2901470/1‑150 (MQ=255)
                                 cGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcggc                                                                                                                 <  2:6580394/150‑1 (MQ=255)
                                      tCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCaa                                                                                                            <  2:4674055/150‑1 (MQ=255)
                                         ggTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCt                                                                                                         <  2:3360816/150‑1 (MQ=255)
                                           tCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTgg                                                                                                       <  2:6091911/150‑1 (MQ=255)
                                              aGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCga                                                                                                    <  1:3658495/150‑1 (MQ=255)
                                              aGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCga                                                                                                    <  1:6560353/150‑1 (MQ=255)
                                              aGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCga                                                                                                    >  2:1710427/1‑150 (MQ=255)
                                               gggTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgat                                                                                                   >  2:2562289/1‑150 (MQ=255)
                                                 gtgtTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatgg                                                                                                 >  1:8257648/1‑150 (MQ=255)
                                                 gtgtTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatgg                                                                                                 >  1:5632211/1‑150 (MQ=255)
                                                   gttgtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatggat                                                                                               >  2:6319482/1‑150 (MQ=255)
                                                      gtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTc                                                                                            <  2:5268507/150‑1 (MQ=255)
                                                          gCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCgg                                                                                        <  1:8385722/150‑1 (MQ=255)
                                                          gCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCgg                                                                                        <  1:2785456/150‑1 (MQ=255)
                                                          gCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGAGGGATGTCGCgg                                                                                        >  2:1075620/1‑150 (MQ=255)
                                                                cccTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGc                                                                                  <  2:1964659/150‑1 (MQ=255)
                                                                cccTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAAGTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGc                                                                                  <  2:7533789/150‑1 (MQ=255)
                                                                  ctgctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTg                                                                                <  2:4760485/150‑1 (MQ=255)
                                                                   tgctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTgg                                                                               <  1:1442964/150‑1 (MQ=255)
                                                                   tgctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTgg                                                                               <  1:4774837/150‑1 (MQ=255)
                                                                         gcgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagcagc                                                                         <  1:671533/150‑1 (MQ=255)
                                                                         gcgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagcagc                                                                         >  2:10528943/1‑150 (MQ=255)
                                                                         gcgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagcagc                                                                         <  1:4197822/150‑1 (MQ=255)
                                                                          cgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCg                                                                        >  2:9097188/1‑150 (MQ=255)
                                                                          cgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCg                                                                        >  2:490931/1‑150 (MQ=255)
                                                                           gcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGt                                                                       >  1:5366625/1‑150 (MQ=255)
                                                                             tGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTgg                                                                     >  1:10164768/1‑150 (MQ=255)
                                                                                  gcgAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGggcggc                                                                <  1:4386743/150‑1 (MQ=255)
                                                                                    gAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAg                                                              >  1:9261721/1‑150 (MQ=255)
                                                                                      ggTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGt                                                            >  1:9905654/1‑150 (MQ=255)
                                                                                        tGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTgg                                                          <  1:7015026/150‑1 (MQ=255)
                                                                                         gCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGc                                                         <  1:4089592/150‑1 (MQ=255)
                                                                                            ggAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCttt                                                      <  2:9716493/150‑1 (MQ=255)
                                                                                               gTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCGTtcgt                                                   >  2:3960915/1‑150 (MQ=255)
                                                                                                tGGGACGATATGGTTGCGGACTACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgtc                                                  <  2:2321449/150‑1 (MQ=255)
                                                                                                tGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgtc                                                  <  1:4601551/150‑1 (MQ=255)
                                                                                                tGGGACGATATGGTTGCGGAATACGCCGAGCAATGGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgtc                                                  <  2:6674554/150‑1 (MQ=255)
                                                                                                  ggACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCgg                                                >  1:11088932/1‑150 (MQ=255)
                                                                                                  ggACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGGTTTCAACCTGGCGGGAGGGTCGCTCGGCGGCACCCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCgg                                                >  2:3106424/1‑150 (MQ=255)
                                                                                                      gATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTgg                                            >  2:4238503/1‑150 (MQ=255)
                                                                                                       atatGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGa                                           >  2:10050650/1‑150 (MQ=255)
                                                                                                       atatGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGa                                           >  1:75288/1‑150 (MQ=255)
                                                                                                        tatGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGATGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGAt                                          >  2:11101897/1‑150 (MQ=255)
                                                                                                         atGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGATTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATc                                         <  2:5986864/150‑1 (MQ=255)
                                                                                                            gTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGAt                                      >  2:5025182/1‑150 (MQ=255)
                                                                                                             ttGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATg                                     <  2:2723310/150‑1 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                    <  1:10682070/150‑1 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                    >  2:8034253/1‑150 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                    >  2:5735875/1‑150 (MQ=255)
                                                                                                                cGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgcgc                                  <  1:4988113/150‑1 (MQ=255)
                                                                                                                 ggAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGcc                                 <  2:2938318/150‑1 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                                >  2:10170581/1‑150 (MQ=255)
                                                                                                                   aaTACGCCGTGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCgg                               >  1:862978/1‑150 (MQ=255)
                                                                                                                    aTACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGc                              <  1:9101258/150‑1 (MQ=255)
                                                                                                                    aTACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGAGCGATGCGCCGGc                              >  2:9361767/1‑150 (MQ=255)
                                                                                                                      aCGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAc                            <  2:1194327/150‑1 (MQ=255)
                                                                                                                      aCGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAc                            >  2:9302804/1‑150 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                           >  1:3387238/1‑150 (MQ=255)
                                                                                                                        gCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCg                          <  2:8504104/150‑1 (MQ=255)
                                                                                                                               aaTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAggg                   <  1:2622068/150‑1 (MQ=255)
                                                                                                                               aaTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAggg                   <  1:1895274/150‑1 (MQ=255)
                                                                                                                               aaTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAggg                   >  1:1602644/1‑150 (MQ=255)
                                                                                                                                aTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGt                  >  1:7209522/1‑150 (MQ=255)
                                                                                                                                   gctgcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGa               <  1:3954108/150‑1 (MQ=255)
                                                                                                                                    ctgcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGaa              >  2:8957729/1‑150 (MQ=255)
                                                                                                                                       cAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCg           >  2:383433/1‑150 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt       <  1:10202170/150‑1 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt       <  1:8211004/150‑1 (MQ=255)
                                                                                                                                             cACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTgg     >  1:3841944/1‑150 (MQ=255)
                                                                                                                                              aCCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGa    >  2:10345000/1‑150 (MQ=255)
                                                                                                                                               ccccGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGaa   >  1:6397088/1‑150 (MQ=255)
                                                                                                                                                cccGAAGGGGATTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAAc  <  1:5727068/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAAC  >  NC_008463/2985198‑2985491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑