Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,373,667 G→C V352V (GTC→GTG)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,373,6670GC100.0% 1753.2 / NA 449V352V (GTC→GTG) PA14_RS15385aminotransferase class V‑fold PLP‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (219/230);  total (219/230)

Only 100 of 454 total aligned reads displayed.

AGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGT  >  NC_008463/3373520‑3373813
                                                                                                                                                   |                                                                                                                                                  
aGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTCTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                  >  2:9298864/1‑150 (MQ=255)
aGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                  <  1:10229970/150‑1 (MQ=255)
  ggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGc                                                                                                                                                <  1:7140236/150‑1 (MQ=255)
   gCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCt                                                                                                                                               <  1:9648099/150‑1 (MQ=255)
   gCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGAGACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCCCGCt                                                                                                                                               <  2:10279444/150‑1 (MQ=255)
     gggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGc                                                                                                                                             <  1:11091192/150‑1 (MQ=255)
            gccggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAgc                                                                                                                                      >  1:1831287/1‑150 (MQ=255)
               ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                   >  1:1566431/1‑150 (MQ=255)
                  cgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggc                                                                                                                                >  1:5786244/1‑150 (MQ=255)
                       aGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCAt                                                                                                                           <  2:8759494/150‑1 (MQ=255)
                        gCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATc                                                                                                                          >  2:10732436/1‑150 (MQ=255)
                        gCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATc                                                                                                                          >  1:4024108/1‑150 (MQ=255)
                        gCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATc                                                                                                                          >  2:4877200/1‑150 (MQ=255)
                         cccgCCCTCGAGACCCCGAGCAACGGCTGCCCCGCCGGCCCGCCACCATCCCCCGCGGCAGTGAGGGGGGCCACCGCCGCGTCGACGTCGCCCTCGCGGGTGAATCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCg                                                                                                                         <  2:4226749/147‑1 (MQ=255)
                         ccTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCg                                                                                                                         >  2:5990782/1‑150 (MQ=255)
                          cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGt                                                                                                                        >  1:2630302/1‑150 (MQ=255)
                          cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGt                                                                                                                        <  1:3745016/150‑1 (MQ=255)
                            gCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTgg                                                                                                                      <  1:6298388/150‑1 (MQ=255)
                              ccTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGcc                                                                                                                    <  1:7469284/150‑1 (MQ=255)
                                tGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGa                                                                                                                  <  1:8421615/150‑1 (MQ=255)
                                    tACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGc                                                                                                              <  2:1524633/150‑1 (MQ=255)
                                     aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCg                                                                                                             <  2:5447142/150‑1 (MQ=255)
                                     aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCg                                                                                                             <  1:6021090/150‑1 (MQ=255)
                                       cccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgag                                                                                                           >  2:6276549/1‑150 (MQ=255)
                                       cccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgag                                                                                                           >  2:6348474/1‑150 (MQ=255)
                                        ccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgaga                                                                                                          <  2:8441804/150‑1 (MQ=255)
                                           aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGc                                                                                                       >  1:9181254/1‑150 (MQ=255)
                                           aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGc                                                                                                       >  2:10219419/1‑150 (MQ=255)
                                           aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGAGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGATGc                                                                                                       >  2:725231/1‑150 (MQ=255)
                                             gAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGTCCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCAc                                                                                                     <  1:4331637/150‑1 (MQ=255)
                                              aaCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGCGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACg                                                                                                    >  2:2585021/1‑150 (MQ=255)
                                                cGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTg                                                                                                  >  1:7309433/1‑150 (MQ=255)
                                                  gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                <  2:1932001/150‑1 (MQ=255)
                                                  gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                <  1:1723245/150‑1 (MQ=255)
                                                    tGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGAc                                                                                              <  2:5737609/150‑1 (MQ=255)
                                                     gCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACg                                                                                             >  2:11144252/1‑150 (MQ=255)
                                                      ccACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACgg                                                                                            >  1:2658133/1‑150 (MQ=255)
                                                         cGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGgcgc                                                                                         <  1:1284678/150‑1 (MQ=255)
                                                         cGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGgcgc                                                                                         <  2:4908064/150‑1 (MQ=255)
                                                           ccGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccg                                                                                       >  1:8647119/1‑150 (MQ=255)
                                                           ccGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccg                                                                                       <  2:3129821/150‑1 (MQ=255)
                                                            cGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccgg                                                                                      >  1:7993984/1‑150 (MQ=255)
                                                                   ccACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGCCGGCGCCGGCCGCGcc                                                                               >  2:5284494/1‑150 (MQ=255)
                                                                   ccACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGcc                                                                               >  1:11075271/1‑150 (MQ=255)
                                                                    cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCg                                                                              <  1:7094556/150‑1 (MQ=255)
                                                                      cTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                            <  2:4058892/150‑1 (MQ=255)
                                                                       tATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGt                                                                           <  1:5380308/150‑1 (MQ=255)
                                                                          ccgccgCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGCCCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGc                                                                        <  1:2372271/150‑1 (MQ=255)
                                                                            gccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAg                                                                      <  2:3526446/150‑1 (MQ=255)
                                                                             ccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAgg                                                                     <  1:6396186/150‑1 (MQ=255)
                                                                              cgcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCTTGTTTCTGGTGCTCGGTGCGGCGAGATCTTGAGGGTTCGTGTCAGTGGTGGAGAAGCACTTGGTATGGCGACGTGCGCGCCGAGTTGAAGGc                                                                    <  2:4454136/150‑1 (MQ=255)
                                                                               gcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcc                                                                   >  1:3305653/1‑150 (MQ=255)
                                                                               gcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGAGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGGTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcg                                                                   >  2:9487635/1‑149 (MQ=255)
                                                                                  gCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAg                                                                <  2:6638259/150‑1 (MQ=255)
                                                                                   ccTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAgg                                                               >  2:9827832/1‑150 (MQ=255)
                                                                                      tGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGc                                                            <  1:5046331/150‑1 (MQ=255)
                                                                                      tGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGc                                                            <  2:1962043/150‑1 (MQ=255)
                                                                                         gggCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTgg                                                         <  1:52139/150‑1 (MQ=255)
                                                                                         gggCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTgg                                                         >  1:3461103/1‑150 (MQ=255)
                                                                                            cggcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGaca                                                      <  1:2975148/150‑1 (MQ=255)
                                                                                                cACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGc                                                  <  1:8876701/150‑1 (MQ=255)
                                                                                                  ccgccgCGTCGACGTCTGCCACGCGCTTGTAGCGAACGAGCCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCCAGGGCGAGAAGCACGAGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGACa                                                >  2:6804985/1‑150 (MQ=255)
                                                                                                  ccgccgCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCa                                                >  2:7391544/1‑150 (MQ=255)
                                                                                                   cgccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAg                                               <  2:1261143/150‑1 (MQ=255)
                                                                                                    gccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAgg                                              <  1:6838742/150‑1 (MQ=255)
                                                                                                     ccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGc                                             <  2:2428345/150‑1 (MQ=255)
                                                                                                     ccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGc                                             >  1:7641101/1‑150 (MQ=255)
                                                                                                       gcgTCGACGTCGGCCTCGCGGGTGGAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTg                                           <  2:10134951/150‑1 (MQ=255)
                                                                                                        cgTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTgg                                          >  2:10860843/1‑150 (MQ=255)
                                                                                                         gTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGa                                         >  1:1447093/1‑150 (MQ=255)
                                                                                                          tCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGAc                                        >  2:1793956/1‑150 (MQ=255)
                                                                                                          tCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGAc                                        >  1:9927098/1‑150 (MQ=255)
                                                                                                               gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAg                                   <  2:7771341/150‑1 (MQ=255)
                                                                                                                    ccTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                              <  2:9019006/150‑1 (MQ=255)
                                                                                                                    ccTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                              <  1:2868257/150‑1 (MQ=255)
                                                                                                                      tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                            <  1:3912189/150‑1 (MQ=255)
                                                                                                                      tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                            <  2:3715138/150‑1 (MQ=255)
                                                                                                                      tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                            <  1:10784495/150‑1 (MQ=255)
                                                                                                                       cgcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                           <  2:2984703/150‑1 (MQ=255)
                                                                                                                       cgcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGAGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                           >  2:5899121/1‑150 (MQ=255)
                                                                                                                        gcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                          <  1:2028616/150‑1 (MQ=255)
                                                                                                                        gcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                          <  2:7896477/150‑1 (MQ=255)
                                                                                                                         cgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggggg                         >  1:4241544/1‑150 (MQ=255)
                                                                                                                           ggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                       <  1:10621404/150‑1 (MQ=255)
                                                                                                                           ggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                       >  2:8335196/1‑150 (MQ=255)
                                                                                                                            gtgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                      <  2:3338924/150‑1 (MQ=255)
                                                                                                                              gtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaaa                    <  2:9587890/150‑1 (MQ=255)
                                                                                                                               tAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                   <  1:2494227/150‑1 (MQ=255)
                                                                                                                               tAGCGACCGAGGCTCAGGCGCACGCGGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                   <  1:7655497/150‑1 (MQ=255)
                                                                                                                                aGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACTTGGTACGGCGCAGGCCTCGGCGAGTTGCAGGCCGAGGTGCTGGACACCGCAAGGCTGGACTCCAGTGCCTGGGGGACAAAgg                  <  2:7618768/150‑1 (MQ=255)
                                                                                                                                   gACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCg               >  1:4661783/1‑150 (MQ=255)
                                                                                                                                   gACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCg               <  2:3944216/150‑1 (MQ=255)
                                                                                                                                     ccGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCggg             >  1:6714314/1‑150 (MQ=255)
                                                                                                                                        aGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg          >  2:8562820/1‑150 (MQ=255)
                                                                                                                                         ggCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGt         <  1:7045779/150‑1 (MQ=255)
                                                                                                                                         ggCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCGGGGGGACAAAGGCCGGGTTGt         >  1:310429/1‑150 (MQ=255)
                                                                                                                                           cTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCg       <  1:10015092/150‑1 (MQ=255)
                                                                                                                                            tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGa      <  1:119057/150‑1 (MQ=255)
                                                                                                                                                gcgcACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAACGGCCGGGTTGTCGAAGGt  <  2:4772357/150‑1 (MQ=255)
                                                                                                                                                gcgcACGCTGCGGCCAGCGCGGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGt  <  1:225437/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGT  >  NC_008463/3373520‑3373813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑