BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,424,152	C→G	Q421E (CAA→GAG)	mexY →	multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,424,152	0	C	G	100.0%	1677.8 / NA	433	Q421E (CAA→GAG)	mexY	multidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (233/200); total (233/200)

Only 100 of 433 total aligned reads displayed.

GTGGCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTT  >  NC_008463/3424008‑3424297

gTGGCCCTGCTCGGCACCTTCACGGTGAGGCTCGGCCTGGGCTTCTCGATCACCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCCTCCTGGTGGACGACGCGATCCTCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGaggaa                                                                                                                                              <  1:4237987/150‑1 (MQ=25)

   gCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGc                                                                                                                                           >  2:10571742/1‑150 (MQ=255)

    cccTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGcc                                                                                                                                          <  1:5094841/150‑1 (MQ=255)

      cTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTg                                                                                                                                        <  2:2699210/150‑1 (MQ=255)

                ccTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACg                                                                                                                              >  1:3361557/1‑150 (MQ=255)

                  ttCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGac                                                                                                                            <  2:5312448/150‑1 (MQ=255)

                    cACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGacgc                                                                                                                          <  2:9084761/150‑3 (MQ=255)

                      cGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgcca                                                                                                                        <  2:1395354/150‑1 (MQ=255)

                      cGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGAcgcca                                                                                                                        <  2:4066366/150‑1 (MQ=255)

                          gATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGTCGCGAGCAGCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGt                                                                                                                    >  2:8209136/1‑150 (MQ=21)

                              cTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAg                                                                                                                >  2:4918617/1‑150 (MQ=255)

                               tCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAgg                                                                                                               >  1:10703914/1‑150 (MQ=255)

                                cGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGc                                                                                                              <  2:6203547/150‑1 (MQ=255)

                                  gCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGa                                                                                                            >  2:4561598/1‑150 (MQ=255)

                                  gCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGa                                                                                                            >  1:3988104/1‑150 (MQ=255)

                                   ccTGGGCTTCTCGATCAGCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGAt                                                                                                           <  2:8554658/150‑1 (MQ=255)

                                   ccTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGAt                                                                                                           >  1:10592321/1‑150 (MQ=255)

                                   ccTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGAt                                                                                                           >  1:11146059/1‑150 (MQ=255)

                                      gggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcg                                                                                                        >  1:9089653/1‑150 (MQ=255)

                                       ggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGACGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcgc                                                                                                       >  2:2110590/1‑150 (MQ=255)

                                       ggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcgc                                                                                                       <  1:3294863/150‑1 (MQ=255)

                                       ggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcgc                                                                                                       <  2:6808703/150‑1 (MQ=255)

                                       ggCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATgcgc                                                                                                       <  2:9585620/150‑1 (MQ=255)

                                         cTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCa                                                                                                     <  1:351300/150‑1 (MQ=255)

                                           tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                   >  1:4823989/1‑148 (MQ=255)

                                           tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                   <  1:5781353/150‑3 (MQ=255)

                                           tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                   >  2:3513329/1‑148 (MQ=255)

                                           tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                   <  1:3000420/150‑3 (MQ=255)

                                           tctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCaga                                                                                                   >  1:1309168/1‑148 (MQ=255)

                                            ctcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGAt                                                                                                  <  2:7551142/150‑1 (MQ=255)

                                             tcGATCACCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATc                                                                                                 <  2:3703645/150‑1 (MQ=21)

                                             tcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATc                                                                                                 >  1:10234569/1‑150 (MQ=255)

                                              cGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCa                                                                                                >  2:10331963/1‑150 (MQ=255)

                                                aTCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGc                                                                                              <  2:10263987/150‑1 (MQ=255)

                                                   aaCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCggg                                                                                           >  1:10003299/1‑150 (MQ=255)

                                                   aaCGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCggg                                                                                           >  1:7545685/1‑150 (MQ=255)

                                                     cGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGc                                                                                         <  1:2797287/150‑1 (MQ=255)

                                                      gTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGcc                                                                                        <  2:6369930/150‑1 (MQ=255)

                                                       tGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCa                                                                                       <  1:6906919/150‑1 (MQ=255)

                                                        gCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAt                                                                                      <  2:8279539/150‑1 (MQ=255)

                                                        gCTGACCATGTTCGGCAGGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAt                                                                                      <  2:4449258/150‑1 (MQ=21)

                                                          tGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcg                                                                                    <  1:1035848/150‑1 (MQ=255)

                                                           gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                   <  1:2372253/150‑1 (MQ=255)

                                                             ccATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtcg                                                                                 <  1:3773125/150‑1 (MQ=255)

                                                              cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                <  2:5171435/150‑1 (MQ=255)

                                                              cATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCgg                                                                                >  2:1631604/1‑150 (MQ=255)

                                                                 gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                             <  2:3883715/150‑1 (MQ=255)

                                                                 gTTCGGCATGGTCCTGGCGATCGGCATCCTGGAGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                             >  1:2113446/1‑150 (MQ=21)

                                                                     ggCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAcc                                                                         >  1:6210805/1‑150 (MQ=255)

                                                                       cATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgt                                                                       >  1:7300894/1‑150 (MQ=255)

                                                                        aTGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtg                                                                      >  2:10129772/1‑150 (MQ=255)

                                                                         tGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtgg                                                                     >  2:9837392/1‑150 (MQ=255)

                                                                             ccTGGCGATCGGCATCCCGGTGGACGACGCGATCATCGTGGTGGAGATCGTCGAGCGGCTGATGGCCGAGGATGGCCTGTAGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCt                                                                 <  1:4400518/150‑1 (MQ=255)

                                                                               tGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTgg                                                               <  1:7726/150‑1 (MQ=255)

                                                                                   gATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGGGGTGCTGGtctc                                                           >  2:2422867/1‑150 (MQ=25)

                                                                                     tCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCTCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAGCACCGTGGTGCTGGTCTCgg                                                         >  2:2656376/1‑150 (MQ=25)

                                                                                     tCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAGCGTGGTGCTGGTCTCgg                                                         >  2:5965469/1‑150 (MQ=25)

                                                                                      cGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGt                                                        <  2:2908451/150‑1 (MQ=255)

                                                                                       ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                       >  1:10842081/1‑150 (MQ=255)

                                                                                        gCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAt                                                      <  1:3626009/150‑1 (MQ=255)

                                                                                         cATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAtt                                                     >  1:1411796/1‑150 (MQ=255)

                                                                                          aTCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTc                                                    >  1:4449855/1‑150 (MQ=255)

                                                                                             cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                 <  1:2837324/150‑1 (MQ=255)

                                                                                             cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                 <  2:2799473/150‑1 (MQ=255)

                                                                                             cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                 <  1:6993747/150‑1 (MQ=255)

                                                                                               ggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCCGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGcc                                               >  2:10746516/1‑150 (MQ=37)

                                                                                                  ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                            <  2:4195719/150‑1 (MQ=255)

                                                                                                   gacgacGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATg                                           >  2:7987040/1‑150 (MQ=255)

                                                                                                    acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                          <  2:3196731/150‑1 (MQ=255)

                                                                                                    acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                          <  1:8890930/150‑1 (MQ=255)

                                                                                                     cgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGc                                         <  1:679005/150‑1 (MQ=255)

                                                                                                       acgCGATCATCGTGGGGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGt                                       >  2:9452920/1‑150 (MQ=38)

                                                                                                        cgcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGtt                                      >  1:8077824/1‑150 (MQ=255)

                                                                                                        cgcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGAATTCGTGCCGATGGCGtt                                      >  2:2255489/1‑150 (MQ=38)

                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                  >  1:8050200/1‑150 (MQ=255)

                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                  >  1:5368993/1‑150 (MQ=255)

                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                  <  1:4349613/150‑1 (MQ=255)

                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                  <  1:5238445/150‑1 (MQ=255)

                                                                                                            atcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttctgc                                  >  2:481906/1‑148 (MQ=255)

                                                                                                               atcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgc                               >  1:9504116/1‑150 (MQ=255)

                                                                                                                 cGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcgg                             <  2:6445773/150‑1 (MQ=255)

                                                                                                                     gtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGcgcg                         <  1:6073029/150‑1 (MQ=255)

                                                                                                                      tggAGAACGTCGAGCGGCTGAGGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCgg                        <  2:10860464/150‑1 (MQ=38)

                                                                                                                         agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                     >  1:6418300/1‑150 (MQ=255)

                                                                                                                         agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCAGTCGCCTCACGACGCCACGGTCAAGGCGAAGCGCCAGATCAGCGGGGCCATCGACGGCATCACCGTGGTGCTGGTCTAGGAATACGAGCCGATGGCGTTCTTCAGCGGCTCGGTgg                     >  2:7655896/1‑150 (MQ=255)

                                                                                                                          gaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTggg                    <  2:7442284/150‑1 (MQ=255)

                                                                                                                            aCGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCa                  <  1:5622739/150‑1 (MQ=255)

                                                                                                                             cGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCaa                 <  2:5860489/150‑1 (MQ=255)

                                                                                                                                cGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACAt              >  1:7148246/1‑150 (MQ=255)

                                                                                                                                  aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATACGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt            >  2:726011/1‑150 (MQ=39)

                                                                                                                                   gCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTa           >  1:2003908/1‑150 (MQ=255)

                                                                                                                                     ggCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAcc         >  1:8358745/1‑150 (MQ=255)

                                                                                                                                      gCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCg        >  2:5587277/1‑150 (MQ=255)

                                                                                                                                       cTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGc       <  1:403733/150‑1 (MQ=255)

                                                                                                                                        tGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGcc      <  1:10986220/150‑1 (MQ=255)

                                                                                                                                         gATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCa     >  1:6635773/1‑150 (MQ=255)

                                                                                                                                         gATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCa     >  2:1280461/1‑150 (MQ=255)

                                                                                                                                         gATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCa     >  1:7935233/1‑150 (MQ=255)

                                                                                                                                         gATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCa     >  2:6070150/1‑150 (MQ=255)

                                                                                                                                         gATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCa     >  1:2679742/1‑150 (MQ=255)

GTGGCCCTGCTCGGCACCTTCACGGTGATGCTCGGCCTGGGCTTCTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTT  >  NC_008463/3424008‑3424297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`