Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,443,2920CG99.6% 1797.9 / NA 466P459A (CCC→GCC) PA14_RS15680GntP family permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (233/231);  minor base T (2/0);  total (235/231)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

GGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACC  >  NC_008463/3443145‑3443436
                                                                                                                                                   |                                                                                                                                                
   gggATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                             <  1:1750470/150‑1 (MQ=255)
     gatggatACCCTGCCGCACGACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCgg                                                                                                                                           >  2:186527/1‑150 (MQ=255)
     gatggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTATCTGCCTTACCCAGAGCAAGACCCTCGCCCTGTTCATCGTCATCGGACTGTTCTACGCCACCgg                                                                                                                                           >  2:7206556/1‑150 (MQ=255)
      atggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGc                                                                                                                                          >  2:6060948/1‑150 (MQ=255)
       tggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGcc                                                                                                                                         >  1:6299405/1‑150 (MQ=255)
         gatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                       <  1:2061017/150‑1 (MQ=255)
            aCCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTc                                                                                                                                    <  1:10637409/150‑1 (MQ=255)
             cccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCt                                                                                                                                   <  2:1616490/150‑1 (MQ=255)
             cccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCt                                                                                                                                   <  1:3634991/150‑1 (MQ=255)
              ccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTg                                                                                                                                  <  2:1149945/150‑1 (MQ=255)
                 gCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATc                                                                                                                               <  2:5212250/150‑1 (MQ=255)
                 gCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATc                                                                                                                               >  1:9441048/1‑150 (MQ=255)
                     cacaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGcc                                                                                                                           <  2:5775632/150‑1 (MQ=255)
                      acaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGccc                                                                                                                          >  2:201116/1‑150 (MQ=255)
                          cGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttt                                                                                                                      >  2:1784816/1‑150 (MQ=255)
                             cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTc                                                                                                                   <  2:6858483/150‑1 (MQ=255)
                              gcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                  <  1:4457051/150‑1 (MQ=255)
                                 gTGATCACCCTCCTGGCGGTCACCGGCCTGACCCTTCGGGAGGCGTCCAAGGCCATTTTCGGCATGTCCCTGATCAAGACCGTGGCGGTGGTCGTCGTCATCGGACTGGTCTACGCCCCCGGCCTGGTCTGATCCGCCCTTTTTTCATcc                                                                                                               >  2:494548/1‑150 (MQ=255)
                                       aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                         <  2:2568976/150‑1 (MQ=255)
                                       aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                         >  1:5619446/1‑150 (MQ=255)
                                        cccTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                        >  1:9114015/1‑150 (MQ=255)
                                        cccTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                        <  1:8899803/150‑1 (MQ=255)
                                        cccTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                        <  1:10163082/150‑1 (MQ=255)
                                         cctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgag                                                                                                       <  2:8217835/150‑1 (MQ=255)
                                          ctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgaga                                                                                                      >  2:9280285/1‑150 (MQ=255)
                                          ctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACAATTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCAACGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgaga                                                                                                      >  2:10319047/1‑150 (MQ=255)
                                             ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAAc                                                                                                   <  1:4619279/150‑1 (MQ=255)
                                              tGGCGTTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                  <  1:10969336/150‑1 (MQ=255)
                                               ggCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAg                                                                                                 <  1:8752734/150‑1 (MQ=255)
                                                gCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTACTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGc                                                                                                <  1:5080783/150‑1 (MQ=255)
                                                 cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCa                                                                                               <  2:5709829/150‑1 (MQ=255)
                                                    tCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGa                                                                                            >  1:5336827/1‑150 (MQ=255)
                                                     cACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAc                                                                                           <  1:4145225/150‑1 (MQ=255)
                                                      aCCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAcc                                                                                          >  2:6261854/1‑150 (MQ=255)
                                                       ccGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAccc                                                                                         <  2:2185913/150‑1 (MQ=255)
                                                        cGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCt                                                                                        <  2:8488480/150‑1 (MQ=255)
                                                         ggCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                       >  2:9048102/1‑150 (MQ=255)
                                                         ggCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                       >  2:3453702/1‑150 (MQ=255)
                                                          gCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGa                                                                                      >  2:10557969/1‑150 (MQ=255)
                                                              gACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAgg                                                                                  <  1:1501855/150‑1 (MQ=255)
                                                                   aTCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGa                                                                             >  1:11141548/1‑150 (MQ=255)
                                                                   aTCGGGAGGCGTACAAGGACATTTACGGCATAACCCAGATCAAGACCCTGGCGGAGTACATCGACAACGGACTGTTCTACGCCACCGGCCAGCTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATCACCCACAAAGGCAAGa                                                                             >  1:6374265/1‑150 (MQ=255)
                                                                    tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAc                                                                            >  2:6179892/1‑150 (MQ=255)
                                                                    tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAc                                                                            >  1:7252044/1‑150 (MQ=255)
                                                                     cGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAcc                                                                           >  1:3269498/1‑150 (MQ=255)
                                                                      gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGTCAAGACCg                                                                          >  2:3188860/1‑150 (MQ=255)
                                                                      gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                          >  2:10152452/1‑150 (MQ=255)
                                                                         agtagTACAAGGACTTTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGTACTGTTCTTCGCCACCGGCCTTGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                       <  2:10819681/146‑1 (MQ=255)
                                                                         aGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                       >  1:3002594/1‑150 (MQ=255)
                                                                         aGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                       >  1:9692569/1‑150 (MQ=255)
                                                                           gCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTg                                                                     <  1:2853659/150‑1 (MQ=255)
                                                                              tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTc                                                                  >  1:9981156/1‑150 (MQ=255)
                                                                                cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAc                                                                >  2:760082/1‑150 (MQ=255)
                                                                                    gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                            <  1:7759506/150‑1 (MQ=255)
                                                                                    gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                            >  2:6408995/1‑150 (MQ=255)
                                                                                    gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                            >  2:8575886/1‑150 (MQ=255)
                                                                                    gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGGCTGATCCGCCCTGTTTTCATCCGGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                            >  2:8076103/1‑150 (MQ=255)
                                                                                     aCATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGtt                                                           <  2:10398142/150‑1 (MQ=255)
                                                                                      cATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTc                                                          >  2:3864413/1‑150 (MQ=255)
                                                                                         tttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccac                                                       <  1:8061369/150‑1 (MQ=255)
                                                                                             ggCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGc                                                   <  2:1389157/150‑1 (MQ=255)
                                                                                               cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCgg                                                 >  2:8098420/1‑150 (MQ=255)
                                                                                                aTTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGCAACAGCATGACCCTGAAAGGCAAGACCGCCCGGGGCACCGGTTCCACCAGCGGc                                                >  1:5834806/1‑150 (MQ=255)
                                                                                                 ttACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTAAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCa                                               >  2:4078623/1‑150 (MQ=255)
                                                                                                  tACCCTGATCAAGACCCTGGCTGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCAt                                              <  2:4735074/150‑1 (MQ=255)
                                                                                                  tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCAt                                              <  2:5862720/150‑1 (MQ=255)
                                                                                                   aCCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                >  1:4279276/1‑147 (MQ=255)
                                                                                                   aCCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                <  2:4279276/147‑1 (MQ=255)
                                                                                                   aCCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                             >  2:7505089/1‑150 (MQ=255)
                                                                                                     ccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCgg                                           <  1:9549816/150‑1 (MQ=255)
                                                                                                      cTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                          >  1:848757/1‑150 (MQ=255)
                                                                                                      cTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                          <  1:2743996/150‑1 (MQ=255)
                                                                                                       tGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGcc                                         <  1:71146/150‑1 (MQ=255)
                                                                                                        gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                        >  2:5527862/1‑150 (MQ=255)
                                                                                                         aTCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTg                                       >  2:10926406/1‑150 (MQ=255)
                                                                                                         aTCAAGAACCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTg                                       <  2:3276967/150‑1 (MQ=255)
                                                                                                          tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                      >  2:9493586/1‑150 (MQ=255)
                                                                                                          tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                      >  2:7962979/1‑150 (MQ=255)
                                                                                                           cAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTggg                                     <  1:10306722/150‑1 (MQ=255)
                                                                                                            aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGc                                    >  2:6544053/1‑150 (MQ=255)
                                                                                                            aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGc                                    >  2:1468953/1‑150 (MQ=255)
                                                                                                              gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCAt                                  >  1:760854/1‑150 (MQ=255)
                                                                                                                 ccTGGCGGTGGTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGc                               <  2:8715293/150‑1 (MQ=255)
                                                                                                                   tGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGccc                             >  1:2780327/1‑150 (MQ=255)
                                                                                                                      cGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGa                          >  2:2500036/1‑150 (MQ=255)
                                                                                                                        gtgtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGc                        >  2:7800726/1‑150 (MQ=255)
                                                                                                                          gtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCt                      <  1:9270546/150‑1 (MQ=255)
                                                                                                                          gtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCt                      <  1:7367897/150‑1 (MQ=255)
                                                                                                                           ttcttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTg                     >  1:6061476/1‑150 (MQ=255)
                                                                                                                             cttcGTCCTCGGACTGTTATTCGCCACCGTCCTGGTCTGATCCGCCCTTCTTTCATCCTGCGGGAGAAACAGCTTGACCCTGACAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGc                   <  2:9625628/150‑1 (MQ=255)
                                                                                                                             cttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGc                   <  2:6980947/150‑1 (MQ=255)
                                                                                                                                cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGa                <  1:6563025/150‑1 (MQ=255)
                                                                                                                                    aTCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGcc            >  1:1364797/1‑150 (MQ=255)
                                                                                                                                      cGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCgg          <  1:2588265/150‑1 (MQ=255)
                                                                                                                                       ggACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGTGAGTAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgc         <  1:2415340/150‑1 (MQ=255)
                                                                                                                                        gACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgcg        <  1:7043375/150‑1 (MQ=255)
                                                                                                                                           tGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg     >  2:284351/1‑150 (MQ=255)
                                                                                                                                           tGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg     >  2:438447/1‑150 (MQ=255)
                                                                                                                                            gTTCTACGCCACCGGCCTGGTCTGATCCGCCCTCTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGa    >  2:232070/1‑150 (MQ=255)
                                                                                                                                              tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGAcc  <  1:6763339/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACC  >  NC_008463/3443145‑3443436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑