Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,769,180 C→G A44A (GCG→GCC)  pscL ← SctL family type III secretion system stator protein PscL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,769,1800CG99.4% 1900.5 / NA 491A44A (GCG→GCC) pscLSctL family type III secretion system stator protein PscL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (254/234);  minor base T (2/1);  total (256/235)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 505 total aligned reads displayed.

GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326
                                                                                                                                                   |                                                                                                                                                  
  gATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTcggcgg                                                                                                                                                <  2:1888251/150‑1 (MQ=255)
   aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcggc                                                                                                                                               >  1:3250692/1‑150 (MQ=255)
        ccTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCt                                                                                                                                          <  1:8392710/150‑1 (MQ=255)
         cTGCCGGGAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCTGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                         <  1:10141590/150‑1 (MQ=255)
          tGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcg                                                                                                                                        <  1:7970452/150‑1 (MQ=255)
             cGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGt                                                                                                                                     >  2:10419639/1‑150 (MQ=255)
             cGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGt                                                                                                                                     >  2:1330523/1‑150 (MQ=255)
              ggTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTg                                                                                                                                    <  2:5655603/150‑1 (MQ=255)
              ggTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTg                                                                                                                                    >  1:9354654/1‑150 (MQ=255)
               gtagtaGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGc                                                                                                                                   >  1:9916001/1‑150 (MQ=255)
                   tagCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCt                                                                                                                               >  2:7875301/1‑150 (MQ=255)
                       gATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTGcca                                                                                                                           >  1:3142490/1‑150 (MQ=255)
                         ttGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTccacc                                                                                                                         >  1:7747781/1‑150 (MQ=255)
                          ttcatcgcAGTAGCTTTTCATGGATCAGCCCGGCCTGGCGCAGACGCGCGTCCTGAAGGGGGGCCTCCCAGGCCTGGGTCTTCTTCTCCTGGTAAACCTCGTGGGCCTGGCTCTCTTTTTGTGCGGCAGGATCGGGTGACGCTTacaaca                                                                                                                        <  2:7705743/145‑1 (MQ=255)
                          tGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTccacca                                                                                                                        >  1:6667383/1‑150 (MQ=255)
                           gCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAg                                                                                                                       >  2:5821296/1‑150 (MQ=255)
                              gcgcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCg                                                                                                                    >  1:3703192/1‑150 (MQ=255)
                                gcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGt                                                                                                                  <  2:1996811/150‑1 (MQ=255)
                                gcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGt                                                                                                                  >  1:10633829/1‑150 (MQ=255)
                                gcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGt                                                                                                                  >  1:8168465/1‑150 (MQ=255)
                                 cAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGtt                                                                                                                 <  2:4078537/150‑1 (MQ=255)
                                  aGTAGCGTGTCCTGGAACAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCGGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTg                                                                                                                <  2:10289784/150‑1 (MQ=255)
                                   gTAGCGTTTCCTGGCTCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTgg                                                                                                               <  2:8237569/150‑1 (MQ=255)
                                     aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcg                                                                                                             >  1:337524/1‑150 (MQ=255)
                                          ttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAg                                                                                                        <  2:333568/150‑1 (MQ=255)
                                          ttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAg                                                                                                        >  2:8125849/1‑150 (MQ=255)
                                            ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGt                                                                                                      >  2:878600/1‑150 (MQ=255)
                                              tGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                    <  1:8600174/150‑1 (MQ=255)
                                                gATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTc                                                                                                  >  2:10941182/1‑150 (MQ=255)
                                                  tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAACCCTCGTGGGCCTCGCGCGCGATGTCGGCGGCACGCTCGCGGGCCGCTGCCACCAGGCGGTTGGCGCGGAGGTAGTccg                                                                                                >  2:7968119/1‑149 (MQ=255)
                                                  tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTCCTCCTGGTAAAACTAGTGGGCCTCGCGCTCGATTTCGGCGGAACGCTCGAGTGCCGCTTCCACCACGCGGTTGGCGCTGAGGTCGTCCt                                                                                                >  2:3744383/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCTCGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                              >  1:6160376/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                              >  2:2396828/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                              >  1:9182211/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                              >  1:7199648/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTGCTGCTCCTGGTAAACCGCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCggg                                                                                              >  2:10079837/1‑150 (MQ=255)
                                                     gCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                             >  2:2134112/1‑150 (MQ=255)
                                                        cGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGt                                                                                          <  1:2224716/150‑1 (MQ=255)
                                                         ggCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                         >  1:1840871/1‑150 (MQ=255)
                                                          gCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcg                                                                                        <  2:1530356/150‑1 (MQ=255)
                                                            cTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcgcg                                                                                      >  1:6285615/1‑150 (MQ=255)
                                                            cTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcgcg                                                                                      >  1:8849131/1‑150 (MQ=255)
                                                             tGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCgg                                                                                     >  2:2349835/1‑150 (MQ=255)
                                                               gcgcAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCAGGGTCGCGTGCCGCTTCCACCTGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgc                                                                                   >  1:10026953/1‑150 (MQ=255)
                                                                 gcAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgc                                                                                 <  1:5374391/150‑1 (MQ=255)
                                                                   aGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgcgc                                                                               <  1:687418/150‑1 (MQ=255)
                                                                    gACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCa                                                                              >  1:4324129/1‑150 (MQ=255)
                                                                         gcCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAACCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAgg                                                                         <  1:5284593/150‑1 (MQ=255)
                                                                          cctcctCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAggg                                                                        >  2:6815280/1‑150 (MQ=255)
                                                                          cctcctCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAggg                                                                        <  2:9529230/150‑1 (MQ=255)
                                                                            tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                      <  1:1953883/150‑1 (MQ=255)
                                                                            tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                      <  2:6684998/150‑1 (MQ=255)
                                                                               tccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGc                                                                   >  1:425117/1‑150 (MQ=255)
                                                                                  aGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCg                                                                <  2:3548060/150‑1 (MQ=255)
                                                                                   gTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAGCCTCGTGGGCCGCGCGCTCGATTTCGGCGGCACGCGCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGGAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                               >  1:2818508/1‑150 (MQ=255)
                                                                                   gTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                               <  2:7438433/150‑1 (MQ=255)
                                                                                    tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggg                                                              <  1:7282203/150‑1 (MQ=255)
                                                                                    tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggg                                                              <  2:7756601/150‑1 (MQ=255)
                                                                                     ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                             <  1:3824740/150‑1 (MQ=255)
                                                                                        gCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGcc                                                          >  1:9547156/1‑150 (MQ=255)
                                                                                               agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTa                                                   <  1:5846560/150‑1 (MQ=255)
                                                                                                gcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                  >  2:3500459/1‑150 (MQ=255)
                                                                                                  ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                <  1:3497846/150‑1 (MQ=255)
                                                                                                  ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                >  2:6232155/1‑150 (MQ=255)
                                                                                                   cagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgcg                                               <  1:1489972/150‑1 (MQ=255)
                                                                                                   cagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgcg                                               >  1:10181139/1‑150 (MQ=255)
                                                                                                     ggCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAc                                             <  2:11064865/150‑1 (MQ=255)
                                                                                                     ggCGCTTCAGCTCCTGGTAAACCTCGGGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAc                                             <  2:9367760/150‑1 (MQ=255)
                                                                                                        gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTgg                                          >  2:5361615/1‑150 (MQ=255)
                                                                                                         cTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGc                                         >  2:856553/1‑150 (MQ=255)
                                                                                                          ttCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCg                                        <  2:3063907/150‑1 (MQ=255)
                                                                                                            cTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTc                                      <  2:10667758/150‑1 (MQ=255)
                                                                                                              gCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                    <  2:2725360/150‑1 (MQ=255)
                                                                                                                tCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGt                                  <  1:5109966/150‑1 (MQ=255)
                                                                                                                tCCTGGTAAACCGCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGt                                  <  2:4842314/150‑1 (MQ=255)
                                                                                                                 ccTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGtt                                 >  2:10850336/1‑150 (MQ=255)
                                                                                                                  cTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTc                                <  2:4613784/150‑1 (MQ=255)
                                                                                                                     gTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaac                             <  2:59727/150‑1 (MQ=255)
                                                                                                                      tAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaaca                            <  1:2986330/150‑1 (MQ=255)
                                                                                                                        aaCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACaaa                          >  2:2909720/1‑150 (MQ=255)
                                                                                                                         aCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTCGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGGTCAACAAAt                         >  2:9698210/1‑150 (MQ=255)
                                                                                                                          ccTCGTGGGCCTCGCGCTCGATTTCGTCGGCACGCTCGCGTGCCGCTTCCACCAGTCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                        >  2:5057803/1‑150 (MQ=255)
                                                                                                                           cTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGGTCAACAAATgg                       >  1:2568093/1‑150 (MQ=255)
                                                                                                                             cGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGaa                     <  1:1636764/150‑1 (MQ=255)
                                                                                                                             cGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGaa                     <  1:11016541/150‑1 (MQ=255)
                                                                                                                               tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                   <  1:6568437/150‑1 (MQ=255)
                                                                                                                                gggCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCCACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCATCAAATGGAAGCa                  >  1:765825/1‑150 (MQ=255)
                                                                                                                                  gCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCAtt                >  2:7265065/1‑150 (MQ=255)
                                                                                                                                  gCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCAtt                <  1:4983681/150‑1 (MQ=255)
                                                                                                                                   ccTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTc               <  2:4400102/150‑1 (MQ=255)
                                                                                                                                   ccTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTc               <  2:2192321/150‑1 (MQ=255)
                                                                                                                                    cTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCa              >  2:706516/1‑150 (MQ=255)
                                                                                                                                     tCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAt             <  1:2022559/150‑1 (MQ=255)
                                                                                                                                        cgcTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgc          <  1:1245731/150‑1 (MQ=255)
                                                                                                                                         gcTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgct         <  1:10273810/150‑1 (MQ=255)
                                                                                                                                            cGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcgc      >  1:7851120/1‑150 (MQ=255)
                                                                                                                                             gATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATcgctcgct     >  2:916104/1‑150 (MQ=255)
                                                                                                                                              aTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt    <  1:4778579/150‑1 (MQ=255)
                                                                                                                                              aTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt    <  2:1123999/150‑1 (MQ=255)
                                                                                                                                               tttCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTg   >  1:8908655/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑