Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,933,352 C→G P346P (CCC→CCG)  PA14_RS17955 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,933,3520CG100.0% 1915.9 / NA 491P346P (CCC→CCG) PA14_RS17955MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (243/248);  total (243/248)

Only 100 of 498 total aligned reads displayed.

GATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGT  >  NC_008463/3933205‑3933498
                                                                                                                                                   |                                                                                                                                                  
  tCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGa                                                                                                                                                >  2:6986578/1‑150 (MQ=255)
    gTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATg                                                                                                                                              <  1:1922727/150‑1 (MQ=255)
     ttCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                             >  2:5653626/1‑150 (MQ=255)
     ttCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                             >  1:9444396/1‑150 (MQ=255)
     ttCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                             <  2:3810338/150‑1 (MQ=255)
     gtCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                             <  1:10254186/149‑1 (MQ=255)
      tCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGac                                                                                                                                            <  2:5781205/150‑1 (MQ=255)
         cACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGaccac                                                                                                                                         >  2:1981420/1‑150 (MQ=255)
          aCCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGaccacc                                                                                                                                        <  1:6353875/150‑1 (MQ=255)
          aCCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGaccacc                                                                                                                                        <  1:7264425/150‑1 (MQ=255)
              ccTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgca                                                                                                                                    <  2:1408937/150‑1 (MQ=255)
              ccTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgca                                                                                                                                    <  1:5280461/150‑1 (MQ=255)
              ccTTCGAGGTAGCCGGCCTGCGCGGCCCGATCCTGATCGGCTTCGCCTCGGACAACCTGATCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCAGGCGCAGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCCCCGgca                                                                                                                                    >  2:2266627/1‑150 (MQ=21)
                 tCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgcagca                                                                                                                                 <  1:4689939/150‑1 (MQ=255)
                  cGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCAt                                                                                                                                <  2:8584485/150‑1 (MQ=255)
                    aGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTc                                                                                                                              <  2:7451958/150‑1 (MQ=255)
                        aGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGAt                                                                                                                          >  2:9290152/1‑150 (MQ=255)
                          ccGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAg                                                                                                                        <  2:8742291/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       <  1:5541213/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       <  1:930554/150‑1 (MQ=255)
                           cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                       >  2:4033610/1‑150 (MQ=255)
                            ggCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTg                                                                                                                      <  2:5028197/150‑1 (MQ=255)
                             gCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTgg                                                                                                                     <  1:4371401/150‑1 (MQ=255)
                              ccTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                    <  2:2545243/150‑1 (MQ=255)
                              ccTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                    <  1:10004563/150‑1 (MQ=255)
                               cTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTc                                                                                                                   >  1:1077401/1‑150 (MQ=255)
                                 gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                 <  2:3196048/150‑1 (MQ=255)
                                 gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                 <  1:5388883/150‑1 (MQ=255)
                                   tCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGcc                                                                                                               <  1:2951052/150‑1 (MQ=255)
                                   tCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGcc                                                                                                               <  2:630971/150‑1 (MQ=255)
                                   tCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGAACAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGcc                                                                                                               <  1:5023182/150‑1 (MQ=255)
                                     ggCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCctg                                                                                                             >  2:7539003/1‑150 (MQ=255)
                                       cccGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCctgct                                                                                                           >  2:1289898/1‑150 (MQ=255)
                                         cGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGt                                                                                                         >  1:4034115/1‑150 (MQ=255)
                                          gATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGtt                                                                                                        <  2:5514113/150‑1 (MQ=255)
                                          gATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGtt                                                                                                        >  1:6639204/1‑150 (MQ=255)
                                            tCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCa                                                                                                      <  2:2361375/150‑1 (MQ=255)
                                             ccTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCat                                                                                                     <  2:8327473/150‑1 (MQ=255)
                                              cTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatg                                                                                                    <  2:898052/150‑1 (MQ=255)
                                              cTGAGCGGCCTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGTTCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatg                                                                                                    <  2:10072750/150‑1 (MQ=255)
                                               tGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatga                                                                                                   <  2:6197907/150‑1 (MQ=255)
                                                gATCGGCTTCGCCTCGGACACGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatgat                                                                                                  <  1:2362637/150‑1 (MQ=255)
                                                gATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatgat                                                                                                  >  1:7025170/1‑150 (MQ=255)
                                                  tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATgg                                                                                                >  1:9005648/1‑150 (MQ=255)
                                                  tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATgg                                                                                                <  1:8626445/150‑1 (MQ=255)
                                                  tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATgg                                                                                                <  2:7986448/150‑1 (MQ=255)
                                                    ggCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGa                                                                                              >  1:6409053/1‑150 (MQ=255)
                                                             tCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTg                                                                                     <  1:9993553/150‑1 (MQ=255)
                                                              cGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGGTCATGATGGGACTGACCCTGt                                                                                    >  1:4093769/1‑150 (MQ=255)
                                                                gACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTAc                                                                                  <  1:10493430/150‑1 (MQ=255)
                                                                 aCAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGGTCGTCCCGGCGATGACCACCGGCAGCATTCCGATCGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACg                                                                                 >  1:2203035/1‑150 (MQ=255)
                                                                  cAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACgg                                                                                >  2:11100140/1‑150 (MQ=255)
                                                                  cAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACgg                                                                                <  2:8393836/150‑1 (MQ=255)
                                                                   aaGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGAGCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGTTGGGACTGACCCTGTACGGc                                                                               >  2:6793306/1‑150 (MQ=255)
                                                                        gTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGa                                                                          >  2:1139993/1‑150 (MQ=255)
                                                                        gTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGa                                                                          <  1:3894072/150‑1 (MQ=255)
                                                                         ttCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGAc                                                                         >  1:4306275/1‑150 (MQ=255)
                                                                          tCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACt                                                                        >  1:586439/1‑150 (MQ=255)
                                                                           cggcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTc                                                                       <  1:1177895/150‑1 (MQ=255)
                                                                              cgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCgat                                                                    >  1:8145358/1‑150 (MQ=255)
                                                                                cacggcgccTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCgatga                                                                  <  2:2771443/150‑1 (MQ=255)
                                                                                         tGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                         >  2:10628083/1‑150 (MQ=255)
                                                                                         tGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                         >  1:983866/1‑150 (MQ=255)
                                                                                         tGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                         >  2:4808908/1‑150 (MQ=255)
                                                                                          gCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTc                                                        <  2:5979283/150‑1 (MQ=255)
                                                                                          gCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTc                                                        <  2:7764320/150‑1 (MQ=255)
                                                                                               ccTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTccgccg                                                   <  2:10275718/150‑1 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  <  2:3404019/150‑1 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  >  2:3072651/1‑150 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  <  1:5793986/150‑1 (MQ=255)
                                                                                                cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCcgccgc                                                  >  1:3574005/1‑150 (MQ=255)
                                                                                                  gCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCa                                                <  1:313084/150‑1 (MQ=255)
                                                                                                    gTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATc                                              >  2:10935860/1‑150 (MQ=255)
                                                                                                    gTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATc                                              >  2:5219624/1‑150 (MQ=255)
                                                                                                     tGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCg                                             >  1:4220923/1‑150 (MQ=255)
                                                                                                        tCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACt                                          >  2:9729986/1‑150 (MQ=255)
                                                                                                         cAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACtt                                         <  1:8345325/150‑1 (MQ=255)
                                                                                                             ctgctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGc                                     <  2:7257858/150‑1 (MQ=255)
                                                                                                              tgctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCAGCTGTTCATGATGGGACTGACCCAGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTGCGGCa                                    >  2:6402896/1‑150 (MQ=255)
                                                                                                               gctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCAc                                   >  1:7753691/1‑150 (MQ=255)
                                                                                                               gctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCAc                                   >  1:9699948/1‑150 (MQ=255)
                                                                                                                        gACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGgc                          <  2:459484/150‑1 (MQ=255)
                                                                                                                        gACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACGTCGGCACCGGCGAGgc                          >  2:7880051/1‑150 (MQ=255)
                                                                                                                          ccGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGgccg                        >  1:8576431/1‑150 (MQ=255)
                                                                                                                           cGTCTGCCTGGCGCTGTTCGTCCCGGCGAAGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCAGCGACTTCGGCACCGGCGAGgccgc                       >  2:2146082/1‑150 (MQ=255)
                                                                                                                            gTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGgccgcc                      <  1:3470567/150‑1 (MQ=255)
                                                                                                                             tCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGccgccg                     >  1:10078035/1‑150 (MQ=255)
                                                                                                                             tCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGccgccg                     >  1:10537501/1‑150 (MQ=255)
                                                                                                                             tCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGccgccg                     >  1:9170522/1‑150 (MQ=255)
                                                                                                                                gCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCa                  >  1:5937617/1‑150 (MQ=255)
                                                                                                                                 ccTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCAc                 <  2:4185143/150‑1 (MQ=255)
                                                                                                                                  cTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCAcc                >  1:4569645/1‑150 (MQ=255)
                                                                                                                                  cTGGCGCGGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCAACGACTTCGGCACCGGCGAGGCCGCCGGCAcc                <  2:6672155/150‑1 (MQ=255)
                                                                                                                                      cgcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCt            <  1:8724098/150‑1 (MQ=255)
                                                                                                                                        cTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTcc          <  1:5100514/150‑1 (MQ=255)
                                                                                                                                        cTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTcc          <  1:1626668/150‑1 (MQ=255)
                                                                                                                                           ttCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGc       <  2:3542383/150‑1 (MQ=255)
                                                                                                                                             cGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCtt     <  2:9493513/150‑1 (MQ=255)
                                                                                                                                              gTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTc    <  2:1578070/150‑1 (MQ=255)
                                                                                                                                              gTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTc    <  2:4496096/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGT  >  NC_008463/3933205‑3933498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑