Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,093 C→G G290R (GGC→CGC)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0930CG100.0% 2020.6 / NA 514G290R (GGC→CGC) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (257/257);  total (257/257)

Only 100 of 514 total aligned reads displayed.

CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCGA  >  NC_008463/4564946‑4565239
                                                                                                                                                   |                                                                                                                                                  
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                  <  1:8636055/150‑1 (MQ=255)
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                  <  2:3908184/150‑1 (MQ=255)
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                  <  2:4149594/150‑1 (MQ=255)
     cgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGcac                                                                                                                                             >  2:6661281/1‑150 (MQ=255)
      gTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGcaca                                                                                                                                            <  2:1031732/150‑1 (MQ=255)
       tCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACaa                                                                                                                                           >  1:9952913/1‑150 (MQ=255)
       tCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACaa                                                                                                                                           >  2:7066871/1‑150 (MQ=255)
         cgcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACt                                                                                                                                         >  2:8462018/1‑150 (MQ=255)
          gcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTg                                                                                                                                        <  2:5687788/150‑1 (MQ=255)
             cgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCg                                                                                                                                     >  2:9748086/1‑150 (MQ=255)
              gggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGa                                                                                                                                    >  2:4730258/1‑150 (MQ=255)
                gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATa                                                                                                                                  >  1:6016923/1‑150 (MQ=255)
                  gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCCGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                                                                                >  1:179552/1‑150 (MQ=255)
                     tGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAAGGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGccc                                                                                                                             <  1:5840130/150‑1 (MQ=255)
                      gCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCg                                                                                                                            <  1:690713/150‑1 (MQ=255)
                       ccGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGc                                                                                                                           >  2:2126070/1‑150 (MQ=255)
                        cGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGcc                                                                                                                          >  2:10270138/1‑150 (MQ=255)
                          gCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                                                                                                        >  1:5480233/1‑150 (MQ=255)
                            aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                                                                                                                      >  2:1145742/1‑150 (MQ=255)
                            aaTGGGGTAGAGGTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                                                                                                                      <  2:11101083/150‑1 (MQ=255)
                             aTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                                                                     >  2:10893033/1‑150 (MQ=255)
                               ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACACCTGGCGATAGGCCCGCCAGGGGTc                                                                                                                   <  2:10607077/150‑1 (MQ=255)
                                 ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcg                                                                                                                 >  1:890580/1‑150 (MQ=255)
                                   tAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgcg                                                                                                               >  1:3451210/1‑150 (MQ=255)
                                    agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                                                                                                              >  1:5141855/1‑150 (MQ=255)
                                     gagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCa                                                                                                             >  1:7100668/1‑150 (MQ=255)
                                     gagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCa                                                                                                             <  1:9781178/150‑1 (MQ=255)
                                      agTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCAc                                                                                                            <  1:6977947/150‑1 (MQ=255)
                                       gTTCCACCTCGCGACTCTCCGCGTCGTACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACg                                                                                                           <  2:1961602/150‑1 (MQ=255)
                                       gTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACg                                                                                                           <  1:3849422/150‑1 (MQ=255)
                                       gTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACg                                                                                                           <  1:6638237/150‑1 (MQ=255)
                                        ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACgg                                                                                                          >  1:1349665/1‑150 (MQ=255)
                                         tCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                                                                                                         <  1:6771627/150‑1 (MQ=255)
                                           caccacGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGACGCGCCGATCTGGCAGAAGTCCCCCGCTTCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGGACAACTGGGGATAGGCCCGCCAGGGGTCCGCGCACGGCAt                                                                                                       <  1:3732267/150‑1 (MQ=255)
                                               tctcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTg                                                                                                   >  2:7280707/4‑150 (MQ=255)
                                               tCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTg                                                                                                   <  1:2723397/150‑1 (MQ=255)
                                                 gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa                                                                                                 >  1:7754936/1‑150 (MQ=255)
                                                 gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa                                                                                                 <  1:4983389/150‑1 (MQ=255)
                                                  cgACTCTCCGCGTCGTACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg                                                                                                <  2:7221536/150‑1 (MQ=255)
                                                        tcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcgc                                                                                          >  1:11042889/1‑150 (MQ=255)
                                                         ccgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGcgcg                                                                                         >  1:10713248/4‑150 (MQ=255)
                                                          cgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGa                                                                                        <  1:5351458/148‑1 (MQ=255)
                                                          cgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGa                                                                                        <  2:9498722/148‑1 (MQ=255)
                                                            cgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACg                                                                                      <  2:3014155/150‑1 (MQ=255)
                                                            cgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACg                                                                                      <  2:3728276/150‑1 (MQ=255)
                                                             gtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACgg                                                                                     <  1:3782640/150‑1 (MQ=255)
                                                             gtcggACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACgg                                                                                     >  1:2940785/1‑150 (MQ=255)
                                                                     tCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGACGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCt                                                                             <  2:3284755/150‑1 (MQ=255)
                                                                       aaTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGAATCGAGGAAGAAGCGGTACGGGATGGGGTTGCGCTGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTgg                                                                           >  2:2727978/1‑150 (MQ=255)
                                                                        aTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGa                                                                          <  2:5233452/150‑1 (MQ=255)
                                                                        aTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGAATGCTGAAGCTGCGCGACGGTACGATCTGGa                                                                          <  1:10170652/150‑1 (MQ=255)
                                                                         tGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGaa                                                                         >  1:1893390/1‑150 (MQ=255)
                                                                           ccGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAaca                                                                       >  1:2371144/1‑150 (MQ=255)
                                                                           ccGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAaca                                                                       >  2:2233174/1‑150 (MQ=255)
                                                                            cGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAacac                                                                      >  1:5710888/1‑150 (MQ=255)
                                                                             gACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACg                                                                     <  2:4904588/150‑1 (MQ=255)
                                                                             gACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACg                                                                     <  2:558336/150‑1 (MQ=255)
                                                                             gACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACg                                                                     >  1:1475388/1‑150 (MQ=255)
                                                                              aCTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGt                                                                    >  1:7284189/1‑150 (MQ=255)
                                                                                    gCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCgg                                                              >  1:9303551/1‑150 (MQ=255)
                                                                                    gCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCgg                                                              >  2:9042475/1‑150 (MQ=255)
                                                                                      gAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACACCTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGcc                                                            <  2:8228858/150‑1 (MQ=255)
                                                                                      gAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGcc                                                            <  2:1513234/150‑1 (MQ=255)
                                                                                       aaGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGTAACACGTCGCCGGccc                                                           >  1:4323397/1‑150 (MQ=255)
                                                                                       aaGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGccc                                                           >  1:9568943/1‑150 (MQ=255)
                                                                                        aGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCt                                                          <  1:2139827/150‑1 (MQ=255)
                                                                                          cgcCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGAACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCGGAAGATGCGCGACGGGACGATCTGGAACACGTCGCCGGCCCTCa                                                        >  1:2420870/1‑150 (MQ=255)
                                                                                            ccgaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACg                                                      <  2:11041695/147‑1 (MQ=255)
                                                                                             cgaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGt                                                     <  2:8762506/148‑1 (MQ=255)
                                                                                             cgaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGt                                                     >  2:10379900/3‑150 (MQ=255)
                                                                                              gaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTg                                                    >  1:3119619/2‑150 (MQ=255)
                                                                                              gaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTg                                                    <  2:7594891/149‑1 (MQ=255)
                                                                                               aaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTgg                                                   >  2:9237488/1‑150 (MQ=255)
                                                                                               aaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTgg                                                   >  2:507978/1‑150 (MQ=255)
                                                                                                  aGGCAGAAGTCCCCCGCATCGAGGAAGCAGCGGTCCGGGCTGGGGGTGCGCAGGCCCAACTGGCGCTAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGACGCTGCGCGACGGAACGATCTGGAACACGTCGCCGGCCCTCACGTGGGcc                                                <  2:8675846/150‑1 (MQ=255)
                                                                                                   ggCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCt                                               >  2:6779748/1‑150 (MQ=255)
                                                                                                   ggCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCt                                               >  2:4621489/1‑150 (MQ=255)
                                                                                                    gCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTg                                              <  1:803513/150‑1 (MQ=255)
                                                                                                     cAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGc                                             <  2:293156/150‑1 (MQ=255)
                                                                                                       gAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTCCGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAg                                           >  2:6276153/1‑150 (MQ=255)
                                                                                                       gAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAg                                           >  1:4615827/1‑150 (MQ=255)
                                                                                                       gAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAg                                           >  2:1758197/1‑150 (MQ=255)
                                                                                                          gTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGgcg                                        <  2:9947656/150‑1 (MQ=255)
                                                                                                           tCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGgcgc                                       >  1:871601/1‑150 (MQ=255)
                                                                                                               ccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCa                                   >  2:1344356/1‑150 (MQ=255)
                                                                                                                  cATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCt                                <  2:6058173/150‑1 (MQ=255)
                                                                                                                  cATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCt                                >  1:1002032/1‑150 (MQ=255)
                                                                                                                   aTCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTg                               <  1:8383484/150‑1 (MQ=255)
                                                                                                                      gAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCg                            <  1:7194085/150‑1 (MQ=255)
                                                                                                                             aagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAg                     >  1:7478353/1‑150 (MQ=255)
                                                                                                                                 ggTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGTAGCTGCGCGCCGGTCCGTTCTGGACCACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCACAGCTCg                 >  2:3537717/1‑150 (MQ=255)
                                                                                                                                 ggTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCg                 >  1:5274131/1‑150 (MQ=255)
                                                                                                                                   tACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCa               >  2:2545175/1‑150 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGCTAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCGGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCAt              <  2:4241166/150‑1 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTGCGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCAt              >  1:6435656/1‑150 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCACAGCGCGCAt              >  2:5698507/1‑150 (MQ=255)
                                                                                                                                          tGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCa        >  1:10337025/1‑150 (MQ=255)
                                                                                                                                           ggggTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAg       >  1:5424651/1‑150 (MQ=255)
                                                                                                                                               ttGCGCAGGCACAACTGGCGATCGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCg   <  1:410939/150‑1 (MQ=255)
                                                                                                                                                tGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTAGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCGa  <  1:9120190/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCGA  >  NC_008463/4564946‑4565239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑