Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,198,405 T→C intergenic (+31/+37) PA14_RS23800 → / ← mdpA ABC transporter substrate‑binding protein/metallopeptidase MdpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,198,4050TC99.7% 1438.8 / NA 374intergenic (+31/+37)PA14_RS23800/mdpAABC transporter substrate‑binding protein/metallopeptidase MdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (163/210);  minor base A (1/0);  total (164/210)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

ATGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGC  >  NC_008463/5198258‑5198551
                                                                                                                                                   |                                                                                                                                                  
aTGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCctt                                                                                                                                                  <  2:4473400/150‑1 (MQ=255)
aTGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCctt                                                                                                                                                  >  2:11093890/1‑150 (MQ=255)
 tGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGCAGCCCTGGCCCTTg                                                                                                                                                 >  1:5605555/1‑150 (MQ=255)
  gCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGc                                                                                                                                                <  2:7665843/150‑1 (MQ=255)
    ccTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCt                                                                                                                                              >  2:8069719/1‑150 (MQ=255)
     cTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCtt                                                                                                                                             <  1:7750156/150‑1 (MQ=255)
     cTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCtt                                                                                                                                             >  2:2196187/1‑150 (MQ=255)
       ggTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTcc                                                                                                                                           >  2:2974411/1‑150 (MQ=255)
         ttGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcg                                                                                                                                         <  1:27054/150‑1 (MQ=255)
         ttGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcg                                                                                                                                         <  1:1563184/150‑1 (MQ=255)
           gCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcgcg                                                                                                                                       >  1:3236295/1‑150 (MQ=255)
            ccGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGt                                                                                                                                      <  1:11424/150‑1 (MQ=255)
            ccGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGt                                                                                                                                      <  2:9032957/150‑1 (MQ=255)
             cGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTg                                                                                                                                     <  1:3775080/150‑1 (MQ=255)
              gCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGTAGCCCTGGCCCTTGCCTTCCCGCGTgc                                                                                                                                    >  1:6705327/1‑150 (MQ=255)
              gCTCGCGCATCCGTTGCTGTTCGACGCGCAACGCAACAACGTCAGCGGCTACCGCCACAGCCCGATGCCGCCGCGGGACTTTTCGCGGGTGAAGCTGGACTGAACGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgc                                                                                                                                    <  1:5150964/150‑1 (MQ=255)
                 ggCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCATGCCTTCCCGCGTgcggc                                                                                                                                 >  1:762462/1‑150 (MQ=255)
                  gcgcATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGgcggcg                                                                                                                                >  2:2364334/1‑150 (MQ=255)
                    gcATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGtt                                                                                                                              >  2:3178001/1‑150 (MQ=255)
                    gcATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACCCGGGAAGCCCTGGGCCGTGCCATACCGCGGGCGGGGtt                                                                                                                              >  1:9289772/1‑150 (MQ=255)
                     cATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTGTTCGCGGGTGAAGCTGGACTGCTCGCGGCGCCGCTCGCGGGAAGCCCTGGCCCTTGCCGTCCCGCGTgcgggggtt                                                                                                                             >  2:6681410/1‑145 (MQ=255)
                       tCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGACGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATc                                                                                                                           >  2:5931886/1‑150 (MQ=255)
                       tCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTtctc                                                                                                                           >  2:8731481/1‑150 (MQ=255)
                       tCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATc                                                                                                                           >  2:7059247/1‑150 (MQ=255)
                           ttGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGGCCTTGCCTTCCCGCGTGCGGCGGTATCGTTc                                                                                                                       >  1:4473589/1‑150 (MQ=255)
                           ttGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTc                                                                                                                       <  1:8379744/150‑1 (MQ=255)
                               tGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcggcc                                                                                                                   <  2:10529526/150‑1 (MQ=255)
                                   cGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACATTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCt                                                                                                               <  2:8350891/150‑1 (MQ=255)
                                       gcgcAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgc                                                                                                           <  1:9838664/150‑1 (MQ=255)
                                        cgcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgcc                                                                                                          <  1:6119836/150‑1 (MQ=255)
                                        cgcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgcc                                                                                                          <  1:4894762/150‑1 (MQ=255)
                                         gcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccg                                                                                                         <  1:10541351/150‑1 (MQ=255)
                                         gcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccg                                                                                                         <  1:11101596/150‑1 (MQ=255)
                                          caacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccgc                                                                                                        <  1:9569925/150‑1 (MQ=255)
                                            acgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGATGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGccgccg                                                                                                      <  1:723094/150‑1 (MQ=255)
                                             cgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGa                                                                                                     <  1:7142990/150‑1 (MQ=255)
                                              gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCGCAGCCGCCGAg                                                                                                    >  2:1296037/1‑150 (MQ=255)
                                              gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAg                                                                                                    >  2:6867102/1‑150 (MQ=255)
                                                aaGAACGTCAGCGGCTCCCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGc                                                                                                  <  2:890249/150‑1 (MQ=255)
                                                aaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGc                                                                                                  <  2:1945387/150‑1 (MQ=255)
                                                aaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGc                                                                                                  >  1:6376651/1‑150 (MQ=255)
                                                 aGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGcc                                                                                                 >  2:9571096/1‑150 (MQ=255)
                                                 aGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGcc                                                                                                 <  1:7395068/150‑1 (MQ=255)
                                                 aGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGcc                                                                                                 <  2:2802028/150‑1 (MQ=255)
                                                 aGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTAGGCCGGCTCAGCCGCCGAGGcc                                                                                                 >  1:4104365/1‑150 (MQ=255)
                                                  gAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCg                                                                                                >  2:3473395/1‑150 (MQ=255)
                                                   aaCGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGa                                                                                               <  1:9316385/150‑1 (MQ=255)
                                                     cGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAg                                                                                             >  2:5759632/1‑150 (MQ=255)
                                                       tCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAggg                                                                                           >  1:3425047/1‑150 (MQ=255)
                                                         aGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACCGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCTTCCCGCGTGCGGCGTTATCGATCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAt                                                                                         <  2:2077191/150‑1 (MQ=255)
                                                             gCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGGAGCCCGGGCCCGTGCCGTCCCGCGGGCGGCGGTTTCGTTAGGCCGGCGCAGCCGCCGCGGCCGCTGGGTtcgtc                                                                                     >  1:2913857/1‑150 (MQ=255)
                                                              cTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgtcg                                                                                    <  1:3131050/150‑1 (MQ=255)
                                                                aCCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGAt                                                                                  <  1:3365970/150‑1 (MQ=255)
                                                                  cGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCCGGAAGCCCTGGCCCTTGCCTACCCCCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                >  1:11009531/1‑150 (MQ=255)
                                                                   gCATGAGCCCGCTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgga                                                                               <  1:6345037/150‑1 (MQ=255)
                                                                   gCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCTTCCCGCGGGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGAAggt                                                                               >  1:10222999/1‑149 (MQ=255)
                                                                    cATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggac                                                                              <  1:3047752/150‑1 (MQ=255)
                                                                     aTGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggacg                                                                             >  2:4996337/1‑150 (MQ=255)
                                                                              aTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGGCGGCGTTATAGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGtt                                                                    >  1:3253959/1‑150 (MQ=255)
                                                                                gTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTgg                                                                  <  1:4706562/150‑1 (MQ=255)
                                                                                gTCGGCGCGGGACTTTTCGCGGGAGAAGCTGGACTGATCGCTGCGCCGCACGCGGGTAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTgg                                                                  <  1:8629025/150‑1 (MQ=255)
                                                                                  cGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTTGCCCTTGCCTTACCGCGGGCGGCGTTATCGTTCGGACGGCTCAGACGCCGATGGCGAAGGGCTCGTCGATGGACGGACTCGGTTGGGt                                                                >  1:2583848/1‑150 (MQ=255)
                                                                                  cGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGt                                                                <  2:9814376/150‑1 (MQ=255)
                                                                                  cGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGt                                                                <  1:7078601/150‑1 (MQ=255)
                                                                                   ggCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTg                                                               <  1:1723248/150‑1 (MQ=255)
                                                                                       cgGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAAcc                                                           <  1:1445308/150‑1 (MQ=255)
                                                                                         ggACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTAGGTTGGGTGAACCAg                                                         >  2:1014488/1‑150 (MQ=255)
                                                                                         ggACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCATGCCTTACCGCGTGCGGCGGTATCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGGTCGTCGATTGACGGACTCGGTTGGGGGTACCAg                                                         >  1:10710996/1‑150 (MQ=255)
                                                                                          gACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGc                                                        <  1:9240005/150‑1 (MQ=255)
                                                                                              tttCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGGAGGGATCGTCGATGGACGGACGCGGGTGGGTGAACCAGCGTgg                                                    >  1:8154563/1‑150 (MQ=255)
                                                                                                 cgcgGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGtcc                                                 <  2:9475812/150‑1 (MQ=255)
                                                                                                      gTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGtccttcct                                            <  2:8996034/150‑1 (MQ=255)
                                                                                                        gAAGCTGGACTGATCGCGGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTtcctcc                                          <  2:4278473/150‑1 (MQ=255)
                                                                                                           gCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTc                                       <  1:5395735/150‑1 (MQ=255)
                                                                                                            cTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGGTGGGTGAACCAGCGTGGTCCTTCCTCCGTCa                                      >  2:7187889/1‑150 (MQ=255)
                                                                                                              ggACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATg                                    <  1:10409692/150‑1 (MQ=255)
                                                                                                                aCTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTTACGTTCGGCCGGCACAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTa                                  >  2:519164/1‑150 (MQ=255)
                                                                                                                   gATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGaa                               <  2:1262065/150‑1 (MQ=255)
                                                                                                                        cTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGGTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAt                          >  2:4116939/1‑150 (MQ=255)
                                                                                                                        cTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATAGTCGATGGTCGGACTCGGTTGGGGGAACCAGCGGGGTCCTTCCACCGTCATGTAGAAGTGAt                          >  2:4759692/1‑150 (MQ=255)
                                                                                                                         tGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtc                         <  1:657733/150‑1 (MQ=255)
                                                                                                                          gcgcCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtcc                        >  1:4346032/1‑150 (MQ=255)
                                                                                                                               gcACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAg                   <  2:1725111/150‑1 (MQ=255)
                                                                                                                                cACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAgg                  <  1:8833910/150‑1 (MQ=255)
                                                                                                                                    cgGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGaa              >  2:2317029/1‑150 (MQ=255)
                                                                                                                                    cgGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGaa              <  2:5528733/150‑1 (MQ=255)
                                                                                                                                       gAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGTACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGc           >  2:3845773/1‑150 (MQ=255)
                                                                                                                                       gAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCATCCGTCATGTAGAAGTGATCCTCCAGGCGAATGc           <  2:329788/150‑1 (MQ=255)
                                                                                                                                        aaGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGACCCAGCGTGGTCCTTCCTCCGGCATGTCGAAGTGATCCTCCAGGCGAATGcc          >  1:10898306/1‑150 (MQ=255)
                                                                                                                                        aaGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc          >  1:6194393/1‑150 (MQ=255)
                                                                                                                                        aaGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc          >  2:3387236/1‑150 (MQ=255)
                                                                                                                                           cccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGaa       <  2:10728520/150‑1 (MQ=255)
                                                                                                                                           cccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGaa       <  2:1988172/150‑1 (MQ=255)
                                                                                                                                            ccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAAc      >  1:10080428/1‑150 (MQ=255)
                                                                                                                                             cTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt     <  2:4579425/150‑1 (MQ=255)
                                                                                                                                             cTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt     >  2:6270975/1‑150 (MQ=255)
                                                                                                                                              tGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTc    >  1:5957029/1‑150 (MQ=255)
                                                                                                                                                gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGc  <  1:2114653/150‑1 (MQ=255)
                                                                                                                                                gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGc  >  1:2466650/1‑150 (MQ=255)
                                                                                                                                                gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGc  <  2:1391049/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGC  >  NC_008463/5198258‑5198551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑