BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,256,379	C→G	P88A (CCC→GCC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,256,379	0	C	G	100.0%	1473.4 / NA	384	P88A (CCC→GCC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (208/176); total (208/176)

Only 100 of 392 total aligned reads displayed.

GAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAG  >  NC_008463/5256232‑5256522

gaggcCGTCTTCCGGCATGAGCATCGGCAGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCGGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               <  2:5883058/150‑1 (MQ=255)

gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               <  2:10479174/150‑1 (MQ=255)

gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               >  2:9407060/1‑150 (MQ=255)

gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               >  1:2774141/1‑150 (MQ=255)

gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                               <  1:6478526/150‑1 (MQ=255)

     cttcttCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgacaa                                                                                                                                          >  1:1273580/1‑150 (MQ=255)

      ttcttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGACGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCgacaac                                                                                                                                         <  2:9199932/150‑1 (MQ=255)

       tcttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGacaaca                                                                                                                                        >  2:10755946/1‑150 (MQ=255)

        cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAacac                                                                                                                                       >  1:9196523/1‑150 (MQ=255)

          tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGc                                                                                                                                     >  2:11097385/1‑150 (MQ=255)

          tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGc                                                                                                                                     >  1:4130676/1‑150 (MQ=255)

            cGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGccc                                                                                                                                   <  1:6929126/150‑1 (MQ=255)

              gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTc                                                                                                                                 >  2:6985040/1‑150 (MQ=255)

              gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTc                                                                                                                                 >  1:10595539/1‑150 (MQ=255)

              gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTc                                                                                                                                 >  2:395388/1‑150 (MQ=255)

                 tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                              >  2:2460085/1‑150 (MQ=255)

                 tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                              >  1:4808459/1‑150 (MQ=255)

                  gAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCNCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                             >  1:5858053/1‑150 (MQ=255)

                    gCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGc                                                                                                                           <  1:7939795/150‑1 (MQ=255)

                     cATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGcc                                                                                                                          >  2:7286813/1‑150 (MQ=255)

                       tCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCaa                                                                                                                        <  1:2388337/150‑1 (MQ=255)

                          gCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                     >  2:6206768/1‑150 (MQ=255)

                           cTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATc                                                                                                                    <  1:8648038/150‑1 (MQ=255)

                              aTCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                                 <  1:3364073/150‑1 (MQ=255)

                              aTCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                                 <  2:7901809/150‑1 (MQ=255)

                               tCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAgg                                                                                                                <  1:1590157/150‑1 (MQ=255)

                                cTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGc                                                                                                               <  1:4621005/150‑1 (MQ=255)

                                cTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGc                                                                                                               <  1:3017360/150‑1 (MQ=255)

                                 ttCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCt                                                                                                              >  2:4210290/1‑150 (MQ=255)

                                    aCCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAc                                                                                                           <  2:624381/150‑1 (MQ=255)

                                     ccGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcg                                                                                                          <  2:7737502/150‑1 (MQ=255)

                                     ccGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcg                                                                                                          >  2:4335972/1‑150 (MQ=255)

                                       gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgcg                                                                                                        >  1:2693248/1‑150 (MQ=255)

                                        ccATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                                       <  2:6389904/150‑1 (MQ=255)

                                        ccATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                                       >  2:10192961/1‑150 (MQ=255)

                                          aTCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAg                                                                                                     >  1:10060775/1‑150 (MQ=255)

                                          aTCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAg                                                                                                     <  1:4281411/150‑1 (MQ=255)

                                           tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                                    >  2:320049/1‑150 (MQ=255)

                                           tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                                    <  2:4496477/150‑1 (MQ=255)

                                            ctgCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                                   <  1:7527215/148‑1 (MQ=255)

                                            cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                                   >  2:5212525/1‑150 (MQ=255)

                                                gAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGtt                                                                                               >  1:5561510/1‑150 (MQ=255)

                                                  gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                             >  2:2591010/1‑150 (MQ=255)

                                                  gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                             <  2:10977750/150‑1 (MQ=255)

                                                  gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                             <  1:6770004/150‑1 (MQ=255)

                                                      gCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGAt                                                                                         <  2:8760426/150‑1 (MQ=255)

                                                       ccGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATc                                                                                        <  1:5514953/150‑1 (MQ=255)

                                                          cgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCggg                                                                                     >  2:157125/1‑150 (MQ=255)

                                                            aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGca                                                                                   >  2:6696926/1‑150 (MQ=255)

                                                            aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGca                                                                                   >  2:5688964/1‑150 (MQ=255)

                                                            aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGca                                                                                   >  1:3651104/1‑150 (MQ=255)

                                                              ggATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcaca                                                                                 <  2:198314/150‑1 (MQ=255)

                                                              ggATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcaca                                                                                 >  1:902879/1‑150 (MQ=255)

                                                               gATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCacac                                                                                >  2:2190832/1‑150 (MQ=255)

                                                                  gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                             >  2:3282994/1‑150 (MQ=255)

                                                                     ccGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCgg                                                                          <  1:5233796/150‑1 (MQ=255)

                                                                      cGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGc                                                                         >  2:8564897/1‑150 (MQ=255)

                                                                         ttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                                      <  2:1796773/150‑1 (MQ=255)

                                                                          tGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCt                                                                     <  2:7642587/150‑1 (MQ=255)

                                                                           gATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTg                                                                    <  1:486531/150‑1 (MQ=255)

                                                                           gATGTCGTGACGGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTg                                                                    <  2:8268927/150‑1 (MQ=255)

                                                                              gTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATg                                                                 >  1:3524514/1‑150 (MQ=255)

                                                                               tCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACCACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                                >  1:9542189/1‑150 (MQ=255)

                                                                               tCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                                >  2:8303004/1‑150 (MQ=255)

                                                                                 gTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAAAATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtct                                                              >  2:10023152/1‑150 (MQ=255)

                                                                                    aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                           <  1:1150251/150‑1 (MQ=255)

                                                                                    aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                           <  2:811916/150‑1 (MQ=255)

                                                                                     cTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCg                                                          <  2:11170685/150‑1 (MQ=255)

                                                                                         gtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACCCGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGca                                                      <  2:2056040/150‑1 (MQ=255)

                                                                                         gtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGca                                                      >  2:5597515/1‑150 (MQ=255)

                                                                                         gtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGca                                                      <  2:5257993/150‑1 (MQ=255)

                                                                                           cGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcacc                                                    <  2:8846740/150‑1 (MQ=255)

                                                                                            ggAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGCCAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcacca                                                   >  2:1330912/1‑150 (MQ=255)

                                                                                               aGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTg                                                >  2:3717794/1‑150 (MQ=255)

                                                                                                   aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAcc                                            >  1:5316977/1‑150 (MQ=255)

                                                                                                       aaGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGa                                        >  1:10356267/1‑150 (MQ=255)

                                                                                                        aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                       >  1:380342/1‑150 (MQ=255)

                                                                                                        aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                       >  2:9022157/1‑150 (MQ=255)

                                                                                                            cTTCCCGAAGATGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGcc                                   >  2:1500541/1‑150 (MQ=255)

                                                                                                            cTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCAACACTGCACCAGGACCGcc                                   <  1:8927335/150‑1 (MQ=255)

                                                                                                            cTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTAGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGcc                                   >  1:5413580/1‑150 (MQ=255)

                                                                                                             ttCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCt                                  <  1:11098778/150‑1 (MQ=255)

                                                                                                               ccAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCg                                <  2:9872186/150‑1 (MQ=255)

                                                                                                                  gAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAac                             >  2:4040134/1‑150 (MQ=255)

                                                                                                                    aGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGGCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacca                           >  2:7536057/1‑150 (MQ=255)

                                                                                                                     gCTGGCGGGCAGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccac                          >  1:10531041/1‑150 (MQ=255)

                                                                                                                         gCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAgg                      >  1:10114119/1‑150 (MQ=255)

                                                                                                                         gCGGGCTGGCCGACCTCGGCCAGCTCGCCGACACCACTCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAgg                      <  2:5580049/150‑1 (MQ=255)

                                                                                                                          cGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGc                     <  1:8341051/150‑1 (MQ=255)

                                                                                                                             gCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaaa                  >  1:7384246/1‑150 (MQ=255)

                                                                                                                                ggCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATcc               <  1:10787069/150‑1 (MQ=255)

                                                                                                                                  ccTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCct             >  2:5497482/2‑150 (MQ=255)

                                                                                                                                  ccTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCct             >  2:4459269/2‑150 (MQ=255)

                                                                                                                                   cTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCctc            <  1:6057945/150‑1 (MQ=255)

                                                                                                                                    tACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCctct           >  1:1861166/1‑150 (MQ=255)

                                                                                                                                     aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTg          >  2:4956736/1‑150 (MQ=255)

                                                                                                                                      ccTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa         <  1:8815078/150‑1 (MQ=255)

                                                                                                                                      ccTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa         <  1:8135369/150‑1 (MQ=255)

                                                                                                                                         cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt      >  1:9412272/1‑150 (MQ=255)

                                                                                                                                         cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt      <  2:7982205/150‑1 (MQ=255)

GAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAG  >  NC_008463/5256232‑5256522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`