BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,365	T→C	K239E (AAG→GAG)	rlmB ←	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,365	0	T	C	100.0%	1724.7 / NA	464	K239E (AAG→GAG)	rlmB	23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (260/204); total (260/204)

Only 100 of 468 total aligned reads displayed.

AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCG  >  NC_008463/5809221‑5809511

gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                                                                              >  2:9310849‑M2/79‑150 (MQ=255)

gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                                                                              >  2:3712026‑M2/79‑150 (MQ=255)

 caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAgg                                                                                                                                             <  2:2014938‑M2/73‑1 (MQ=255)

   aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                                                                           <  2:8035332‑M2/75‑1 (MQ=255)

     gggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGa                                                                                                                                         <  2:8861921‑M2/77‑1 (MQ=255)

      ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                                                                        >  2:10430916‑M2/73‑150 (MQ=255)

      ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                                                                        <  1:2224896‑M2/78‑1 (MQ=255)

        cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                                                                      <  2:7230203‑M2/80‑1 (MQ=255)

            attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                                                                  >  2:882015‑M2/67‑150 (MQ=255)

                   agaagggcaaccggcgacgcaaggaagattggcgaatatgtgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGAcc                                                                                                                           >  2:1294026‑M2/60‑150 (MQ=255)

                     acgggcacccggcgaagcaaggaagattggcgaatatgtgtacagcggatggaaaggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCga                                                                                                                         <  2:2723943‑M2/93‑1 (MQ=255)

                         gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                                                                     <  1:1980531‑M2/97‑1 (MQ=255)

                         gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                                                                     >  1:6087076‑M2/54‑150 (MQ=255)

                          caaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGt                                                                                                                    <  2:6297301‑M2/98‑1 (MQ=255)

                           aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                                                                   >  2:8160409‑M2/52‑150 (MQ=255)

                            accggcgaagcaaggaagattggcgaatttttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGTCGGCCCCTCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                                                                  <  1:7818360‑M2/100‑1 (MQ=255)

                            accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                                                                  <  2:10258746‑M2/100‑1 (MQ=255)

                            accggcgaagcaaggaagattggcgaatattggtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                                                                  <  1:5624263‑M2/100‑1 (MQ=255)

                               ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                                                                               <  2:9475414‑M2/103‑1 (MQ=255)

                               ggcgaagcaaggaagattggcgaatagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGTACCGGCAGACGCCGGTGGCGACCGAGTCGGTCAgg                                                                                                               >  1:3385625‑M2/48‑150 (MQ=255)

                                 cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                                                                             <  1:8391341‑M2/105‑1 (MQ=255)

                                    agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑GCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCCGACGCCGGTGGCGCCCGAGACGTTCAGgcggct                                                                                                          >  1:262554‑M2/43‑150 (MQ=255)

                                    agcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                                                                          >  2:5247079‑M2/43‑150 (MQ=255)

                                    agcaaggaagattggcgaatatttgtacagcggaaggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                                                                          >  2:10612610‑M2/43‑150 (MQ=255)

                                     gcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGATCAGGctgctg                                                                                                         >  2:9235642‑M2/42‑150 (MQ=255)

                                       aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAc                                                                                                       <  2:7283896‑M2/111‑1 (MQ=255)

                                         ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGc                                                                                                     <  2:2174633‑M2/113‑1 (MQ=255)

                                             gattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                                                                                 >  2:2908995‑M2/34‑150 (MQ=255)

                                              attggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCg                                                                                                <  2:7371679‑M2/118‑1 (MQ=255)

                                               ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCgg                                                                                               >  1:10999522‑M2/32‑150 (MQ=255)

                                                  gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCAGCCGGCCa                                                                                            >  1:1151664‑M2/29‑150 (MQ=255)

                                                   cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGCATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                                                                           >  2:8329634‑M2/28‑150 (MQ=255)

                                                   cgaatatttgtacagcggatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                                                                           <  2:1488112‑M2/123‑1 (MQ=255)

                                                    gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                                                                          <  2:6748938‑M2/124‑1 (MQ=255)

                                                    gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                                                                          <  2:10544310‑M2/124‑1 (MQ=255)

                                                     aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGCTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                         >  2:10211063‑M2/26‑150 (MQ=255)

                                                          ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAc                                                                                    <  2:6374046‑M2/130‑1 (MQ=255)

                                                              acagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                                                                                <  2:8619943‑M2/134‑1 (MQ=255)

                                                                   ggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                                                                           >  2:6176491‑M2/12‑150 (MQ=255)

                                                                      tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCACACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGAGGCGACCGAGACGTTCATGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                                                                        >  2:7904560‑M2/9‑150 (MQ=255)

                                                                       ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                                                                       <  2:5763543‑M2/143‑1 (MQ=255)

                                                                        gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                                                                      >  1:10073770‑M2/7‑150 (MQ=255)

                                                                        gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                                                                      <  2:222776‑M2/144‑1 (MQ=255)

                                                                         aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                                                                     >  1:7113797‑M2/6‑150 (MQ=255)

                                                                         aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                                                                     >  1:7761554‑M2/6‑150 (MQ=255)

                                                                           tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                                                                   >  2:8471865‑M2/4‑150 (MQ=255)

                                                                           tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                                                                   <  1:6399981‑M2/147‑1 (MQ=255)

                                                                           tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGCCGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                                                                   <  2:780352‑M2/147‑1 (MQ=255)

                                                                             gcgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt                                                                 >  1:9909396/1‑150 (MQ=255)

                                                                              cgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTg                                                                >  1:2894134/1‑150 (MQ=255)

                                                                                    ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCTcgc                                                           >  2:4465762/8‑150 (MQ=255)

                                                                                      ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACCTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg                                                         <  1:5283374/145‑1 (MQ=255)

                                                                                        gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                       >  2:4802322/4‑150 (MQ=255)

                                                                                        gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                       >  2:4901766/4‑150 (MQ=255)

                                                                                        gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCAGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                       >  1:37984/4‑150 (MQ=255)

                                                                                          gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa                                                     <  1:4131083/149‑1 (MQ=255)

                                                                                          gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAGCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa                                                     >  1:4294343/2‑150 (MQ=255)

                                                                                           ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg                                                    <  2:5468326/150‑1 (MQ=255)

                                                                                           ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg                                                    >  1:9484834/1‑150 (MQ=255)

                                                                                           ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg                                                    <  2:9367431/150‑1 (MQ=255)

                                                                                            cgcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc                                                   >  1:4877963/7‑150 (MQ=255)

                                                                                             gcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGcc                                                  >  1:8742124/6‑150 (MQ=255)

                                                                                               ggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGTCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCgg                                                <  1:10308917/147‑1 (MQ=255)

                                                                                                gcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgc                                               >  2:2616768/3‑150 (MQ=255)

                                                                                                gcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgc                                               >  1:8176222/3‑150 (MQ=255)

                                                                                                 cggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgcg                                              <  2:7508547/149‑1 (MQ=255)

                                                                                                  ggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgcgc                                             >  2:3348534/1‑150 (MQ=255)

                                                                                                   gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCa                                            >  1:10828155/1‑150 (MQ=255)

                                                                                                    ccccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                           >  2:3127148/1‑150 (MQ=255)

                                                                                                     cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATg                                          <  1:8038788/150‑1 (MQ=255)

                                                                                                          aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                     >  1:7265392/1‑150 (MQ=255)

                                                                                                          aCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                     >  2:1612217/1‑150 (MQ=255)

                                                                                                           cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                    <  1:2370093/150‑1 (MQ=255)

                                                                                                           cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTg                                    >  2:7093612/1‑150 (MQ=255)

                                                                                                             cgcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGcc                                  >  2:3174625/1‑150 (MQ=255)

                                                                                                              gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCt                                 <  2:9027924/150‑1 (MQ=255)

                                                                                                               cTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                >  1:1516519/1‑150 (MQ=255)

                                                                                                                tCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTc                               >  1:4368405/1‑150 (MQ=255)

                                                                                                                  aCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                             >  1:1663818/1‑150 (MQ=255)

                                                                                                                  aCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcg                             >  2:10010859/1‑150 (MQ=255)

                                                                                                                   cTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                            <  2:455044/150‑1 (MQ=255)

                                                                                                                   cTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                            >  1:3239398/1‑150 (MQ=255)

                                                                                                                   cTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                            >  1:1812614/1‑150 (MQ=255)

                                                                                                                      cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGcc                         <  1:8275914/150‑1 (MQ=255)

                                                                                                                      cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGcc                         <  1:757240/150‑1 (MQ=255)

                                                                                                                       aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCCGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                        >  2:9591008/1‑150 (MQ=255)

                                                                                                                       aGCGGGGCGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                        <  2:10157297/150‑1 (MQ=255)

                                                                                                                        gCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCa                       <  2:3864816/150‑1 (MQ=255)

                                                                                                                           gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCa                    <  1:6896874/150‑1 (MQ=255)

                                                                                                                                cgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCTGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGc               <  2:3950836/150‑1 (MQ=255)

                                                                                                                                 gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa              <  2:4191813/150‑1 (MQ=255)

                                                                                                                                 gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa              >  2:1745101/1‑150 (MQ=255)

                                                                                                                                 gcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCa              <  2:11075006/150‑1 (MQ=255)

                                                                                                                                  cTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCAc             <  2:5644633/150‑1 (MQ=255)

                                                                                                                                     gCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt          <  2:9714532/150‑1 (MQ=255)

                                                                                                                                     gCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt          <  1:4576049/150‑1 (MQ=255)

                                                                                                                                       ggaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCg        <  1:10006614/150‑1 (MQ=255)

                                                                                                                                        gaggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCgg       >  2:6645008/1‑150 (MQ=255)

                                                                                                                                          ggaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGTCGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcc     <  1:5176998/150‑1 (MQ=255)

                                                                                                                                           gaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGccc    <  1:2505911/150‑1 (MQ=255)

AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCG  >  NC_008463/5809221‑5809511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`