Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,968,0250CG99.8% 1823.5 / ‑6.3 470P426A (CCC→GCC) phaCclass II poly(R)‑hydroxyalkanoic acid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (266/203);  minor base T (1/0);  total (267/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

AACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTC  >  NC_008463/5967878‑5968171
                                                                                                                                                   |                                                                                                                                                  
aaCTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgcc                                                                                                                                                  <  1:6653159/150‑1 (MQ=255)
aaCTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgcc                                                                                                                                                  >  2:5647893/1‑150 (MQ=255)
aaCTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgcc                                                                                                                                                  >  2:10555803/1‑150 (MQ=255)
 actactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgccc                                                                                                                                                 >  2:9526116/1‑150 (MQ=255)
   tactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGACCCGCCCCGGCGCCCTg                                                                                                                                               >  2:7087147/1‑150 (MQ=255)
    actGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTgg                                                                                                                                              <  2:638213/150‑1 (MQ=255)
      tGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                            >  2:3295530/1‑150 (MQ=255)
      tGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                            >  1:6786232/1‑150 (MQ=255)
        ggTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGt                                                                                                                                          >  2:1635113/1‑150 (MQ=255)
          tCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCCACCCGCTGAACCGCCCCGGCGCCCTGGAGGtct                                                                                                                                        >  2:7318909/1‑150 (MQ=255)
          tCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGtct                                                                                                                                        >  2:10870612/1‑150 (MQ=255)
             acaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCg                                                                                                                                     >  2:5875814/1‑150 (MQ=255)
                acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACACCGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                  <  2:5111398/150‑1 (MQ=255)
                acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                  >  2:2835315/1‑150 (MQ=255)
                acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                  >  2:3782547/1‑150 (MQ=255)
                 cTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCAc                                                                                                                                 >  1:568001/1‑150 (MQ=255)
                    ccTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGcc                                                                                                                              >  1:3839709/1‑150 (MQ=255)
                     cTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGccc                                                                                                                             >  2:9732564/1‑150 (MQ=255)
                       gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCac                                                                                                                           >  1:103358/1‑149 (MQ=255)
                       gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAt                                                                                                                           <  2:3048256/150‑1 (MQ=255)
                       gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAt                                                                                                                           >  2:8413844/1‑150 (MQ=255)
                               aCCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTGCGTCGATCTGTTCACGTGCTACCCGCTGAACCGCGCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGAGCTGa                                                                                                                   >  2:9346523/1‑150 (MQ=25)
                                 cAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAGCGACCTGAAg                                                                                                                 >  2:8806873/1‑150 (MQ=255)
                                   gccgccGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCa                                                                                                               >  2:7897955/1‑150 (MQ=255)
                                     cgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCTCGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAgg                                                                                                             <  2:4621087/150‑1 (MQ=255)
                                      gccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGt                                                                                                            >  1:97251/1‑150 (MQ=255)
                                      gccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGt                                                                                                            <  1:3354108/150‑1 (MQ=255)
                                           cGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACtt                                                                                                       <  2:1945543/150‑1 (MQ=255)
                                             ttCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                     <  1:1002820/150‑1 (MQ=255)
                                              tCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCAGCACGCCGAGATCGACGAAGAGGTCAAGAGCACCCCGCGGAAGCGCCACCGCTCCCAGCAGTGCAGCGCCGCTCCCCTCGACCTGGAGCAGGTGAcaagcg                                                                                                    >  2:8719900/1‑145 (MQ=16)
                                                 aCATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                 >  2:2223909/1‑150 (MQ=255)
                                                   aTCCTCTACTGGACCAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACGTc                                                                                               >  2:1336580/1‑150 (MQ=255)
                                                   aTCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTc                                                                                               <  1:5875585/150‑1 (MQ=255)
                                                           cTGTAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGAGACTTCTACTgtgt                                                                                       >  2:10436618/1‑150 (MQ=255)
                                                            tGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTc                                                                                      <  1:6171432/150‑1 (MQ=255)
                                                             ggAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTCCTGTGTCg                                                                                     >  2:5336398/1‑150 (MQ=255)
                                                              gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGc                                                                                    <  1:5257732/150‑1 (MQ=255)
                                                               aacaacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGcc                                                                                   >  1:1837020/1‑150 (MQ=255)
                                                                 caacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCgg                                                                                 <  1:2837460/150‑1 (MQ=255)
                                                                 caacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCgg                                                                                 <  2:6331871/150‑1 (MQ=255)
                                                                    cGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCACGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                              >  1:8959993/1‑150 (MQ=255)
                                                                     gACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTg                                                                             <  1:112775/150‑1 (MQ=255)
                                                                      acacCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGa                                                                            <  1:8568653/150‑1 (MQ=255)
                                                                          cacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGa                                                                        <  1:104235/150‑1 (MQ=255)
                                                                          cacGCGCCCGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGa                                                                        <  1:2269866/150‑1 (MQ=255)
                                                                           acGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAc                                                                       >  1:1361876/1‑150 (MQ=255)
                                                                                ccTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                  >  1:7768646/1‑150 (MQ=255)
                                                                                ccTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                  >  2:3856243/1‑150 (MQ=255)
                                                                                ccTGCCCGCCGCGCTGCACGGCGAGTTCGACGAACTGTACAAGAGCAACCCGCAGAACCGCCCCGGCGCCCTGGAGCTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACGTGCGACTTCTCCTGTGTCGCCGGGCTGAACGCCcacag                                                                  >  2:6165274/1‑149 (MQ=255)
                                                                                 cTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATc                                                                 <  2:1014775/150‑1 (MQ=255)
                                                                                 cTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATc                                                                 <  1:3754418/150‑1 (MQ=255)
                                                                                        ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                          >  1:9933969/1‑150 (MQ=255)
                                                                                        ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                          >  2:6614005/1‑150 (MQ=255)
                                                                                        ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                          <  1:3070220/150‑1 (MQ=255)
                                                                                         cgcgCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                         <  2:8636479/150‑1 (MQ=255)
                                                                                         cgcgCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                         <  1:4351691/150‑1 (MQ=255)
                                                                                             cTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                     >  2:2043993/1‑150 (MQ=255)
                                                                                             cTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                     >  1:4136092/1‑150 (MQ=255)
                                                                                              tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGt                                                    <  2:7636457/150‑1 (MQ=255)
                                                                                               gCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTc                                                   >  2:8180035/1‑150 (MQ=255)
                                                                                                 aCGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGt                                                 >  1:2026890/1‑150 (MQ=255)
                                                                                                  cGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCTCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTg                                                >  1:4522819/1‑150 (MQ=255)
                                                                                                   ggCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGc                                               <  1:9220046/150‑1 (MQ=255)
                                                                                                     cGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTa                                             <  2:4115977/150‑1 (MQ=255)
                                                                                                      gAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTAc                                            <  2:1166640/150‑1 (MQ=255)
                                                                                                       aGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGAGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACa                                           >  2:2727296/1‑150 (MQ=255)
                                                                                                        gTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                          >  2:6463346/1‑150 (MQ=255)
                                                                                                        gTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                          <  1:7289258/150‑1 (MQ=255)
                                                                                                          tcgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                        >  2:3670742/1‑150 (MQ=255)
                                                                                                          tcgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                        >  1:6593089/1‑150 (MQ=255)
                                                                                                          tcgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                        <  2:6056937/150‑1 (MQ=255)
                                                                                                            gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGATCGAGCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                      >  1:208493/1‑150 (MQ=255)
                                                                                                            gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                      >  1:10124136/1‑150 (MQ=255)
                                                                                                            gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                      >  1:2584552/1‑150 (MQ=255)
                                                                                                              cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGc                                    >  2:2337195/1‑150 (MQ=255)
                                                                                                              cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGc                                    <  1:8417797/150‑1 (MQ=255)
                                                                                                              cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGc                                    >  1:10206018/1‑150 (MQ=255)
                                                                                                                aaCTGTTCAAGAGCACCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                  >  2:2463036/1‑150 (MQ=255)
                                                                                                                 aCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAg                                 >  1:1585757/1‑150 (MQ=255)
                                                                                                                   tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTTCAAGTCGGCCAGGc                               <  1:759324/150‑1 (MQ=255)
                                                                                                                    gTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCt                              <  1:3637814/150‑1 (MQ=255)
                                                                                                                     ttCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTa                             <  2:10355010/150‑1 (MQ=255)
                                                                                                                      tCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTAc                            <  2:3138797/150‑1 (MQ=255)
                                                                                                                        aaGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTg                          >  2:4798144/1‑150 (MQ=255)
                                                                                                                          gagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTggg                        >  1:7003517/1‑150 (MQ=255)
                                                                                                                            gCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggcg                      >  1:26417/1‑150 (MQ=255)
                                                                                                                                cccGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCaa                  <  1:8043634/150‑1 (MQ=255)
                                                                                                                                  cGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGt                <  2:7510341/150‑1 (MQ=255)
                                                                                                                                   gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTg               <  1:9100963/150‑1 (MQ=255)
                                                                                                                                        aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt          >  1:8139095/1‑150 (MQ=255)
                                                                                                                                         ccGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGtt         <  2:9880063/150‑1 (MQ=255)
                                                                                                                                          cGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGACGGCCAGGCTACTGGGCGGCAAGTGCGAGTTc        <  2:4221160/150‑1 (MQ=255)
                                                                                                                                            ccccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCAt      >  1:6351411/1‑150 (MQ=255)
                                                                                                                                            ccccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCAt      >  1:5022535/1‑150 (MQ=255)
                                                                                                                                             cccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATc     <  1:5514107/150‑1 (MQ=255)
                                                                                                                                             cccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATc     >  2:899993/1‑150 (MQ=255)
                                                                                                                                             cccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATc     >  1:10929800/1‑150 (MQ=255)
                                                                                                                                              ccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATcc    >  1:9515187/1‑150 (MQ=255)
                                                                                                                                              ccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATcc    >  2:7163033/1‑150 (MQ=255)
                                                                                                                                               cGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCct   >  1:4755918/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTC  >  NC_008463/5967878‑5968171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑