BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	757,702	C→T	R680C (CGC→TGC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	757,702	0	C	T	100.0%	1523.7 / NA	355	R680C (CGC→TGC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (147/208); total (147/208)

Only 100 of 361 total aligned reads displayed.

ATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAAACCG  >  NC_008463/757555‑757848

 tGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTtgcg                                                                                                                                                 >  2:5932826/1‑150 (MQ=255)

 tGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTtgcg                                                                                                                                                 >  2:2569450/1‑150 (MQ=255)

      gACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGc                                                                                                                                            >  1:10042525/1‑150 (MQ=255)

      gACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGc                                                                                                                                            >  1:2385829/1‑150 (MQ=255)

        cGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCCGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTa                                                                                                                                          <  1:8124562/150‑1 (MQ=255)

         gTGATGGGCGACCTGAACCGTCGTCGTGGTCTGCTTCAGGGGATGGAGGAAACGGTTTCCGGTCAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTAc                                                                                                                                         >  2:4062844/1‑150 (MQ=255)

         gTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTAc                                                                                                                                         >  2:4710485/1‑150 (MQ=255)

              gggCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCAt                                                                                                                                    <  2:1989856/150‑1 (MQ=255)

                  gACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGaa                                                                                                                                >  1:5852486/1‑150 (MQ=255)

                   aCCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAAt                                                                                                                               <  1:6850419/150‑1 (MQ=255)

                      tGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATtct                                                                                                                            >  2:10550353/1‑150 (MQ=255)

                       gAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATtctc                                                                                                                           >  2:3367651/1‑150 (MQ=255)

                       gAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATtctc                                                                                                                           >  1:7732524/1‑150 (MQ=255)

                        aaCCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTcc                                                                                                                          >  2:8791805/1‑150 (MQ=255)

                           cgtcgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCaaa                                                                                                                       <  1:7202740/150‑1 (MQ=255)

                           cgtcgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCaaa                                                                                                                       >  1:319199/1‑150 (MQ=255)

                             tcgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATa                                                                                                                     >  1:9017077/1‑150 (MQ=255)

                                tcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGc                                                                                                                  <  2:821028/150‑1 (MQ=255)

                                tcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGc                                                                                                                  <  1:9027122/150‑1 (MQ=255)

                                tcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGc                                                                                                                  <  1:6147037/150‑1 (MQ=255)

                                  gtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCg                                                                                                                >  1:9533723/1‑150 (MQ=255)

                                    ggTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGaa                                                                                                              >  2:246035/1‑150 (MQ=255)

                                      tCTGATTCAGGGGAGGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCAGGTCCCTGGGTTGCGCGAGCTACTCGATGGAATTCTCCAATTACGCCGAAGc                                                                                                            >  2:280019/1‑150 (MQ=255)

                                         gATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTcc                                                                                                         <  1:1991664/150‑1 (MQ=255)

                                           ttCAGGGGATGGAGGACACGGTTTCCGGTACGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGTCGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAATTACGCCGAAGCTCCGt                                                                                                       >  2:2274505/1‑150 (MQ=255)

                                           ttCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGt                                                                                                       <  2:7107195/150‑1 (MQ=255)

                                             cAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCg                                                                                                     >  1:2617630/1‑150 (MQ=255)

                                              aGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGa                                                                                                    <  2:4173563/150‑1 (MQ=255)

                                              aGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGa                                                                                                    >  2:4882244/1‑150 (MQ=255)

                                                gggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAAc                                                                                                  >  1:9712194/1‑150 (MQ=255)

                                                 ggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACa                                                                                                 <  2:10758488/150‑1 (MQ=255)

                                                   aTGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtc                                                                                               <  2:6328311/150‑1 (MQ=255)

                                                     ggaggaCACGGTTTCCGGGAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgt                                                                                             <  2:8318192/150‑1 (MQ=255)

                                                      gaggaCACGGTTTCCGGTAAGGTGATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgtc                                                                                            <  2:5995152/150‑1 (MQ=255)

                                                           cacGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgc                                                                                       <  2:9785038/150‑1 (MQ=255)

                                                             cGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgcgc                                                                                     >  1:2109085/1‑150 (MQ=255)

                                                                  tCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGtt                                                                                <  2:2152403/150‑1 (MQ=255)

                                                                   ccGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTa                                                                               >  2:2917309/1‑150 (MQ=255)

                                                                     ggTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                                             <  2:5475481/150‑1 (MQ=255)

                                                                     ggTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                                             >  1:9690482/1‑150 (MQ=255)

                                                                     ggTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                                             >  1:3638495/1‑150 (MQ=255)

                                                                       tAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaa                                                                           <  2:5100625/150‑1 (MQ=255)

                                                                       tAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaa                                                                           >  1:3107981/1‑150 (MQ=255)

                                                                       tAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaa                                                                           <  1:1374944/150‑1 (MQ=255)

                                                                        aaGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaaa                                                                          >  2:8664472/1‑150 (MQ=255)

                                                                         aGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAAc                                                                         <  1:11050110/150‑1 (MQ=255)

                                                                          ggTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACa                                                                        >  2:1519963/1‑150 (MQ=255)

                                                                           gTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACaa                                                                       <  2:8028427/150‑1 (MQ=255)

                                                                            ttATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAg                                                                      <  1:4086109/150‑1 (MQ=255)

                                                                             tATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAgg                                                                     <  1:7547166/150‑1 (MQ=255)

                                                                               ttCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGtt                                                                   <  2:767787/150‑1 (MQ=255)

                                                                               ttCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTAGTTAAAAAACAAGGtt                                                                   >  1:3359845/1‑150 (MQ=255)

                                                                                tCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTg                                                                  <  1:2899303/150‑1 (MQ=255)

                                                                                 cGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGa                                                                 <  2:11093634/150‑1 (MQ=255)

                                                                                  gTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGAt                                                                <  1:3952243/150‑1 (MQ=255)

                                                                                   tGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGAtt                                                               >  2:2200087/1‑150 (MQ=255)

                                                                                   tGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGAtt                                                               >  1:2487845/1‑150 (MQ=255)

                                                                                    gCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTc                                                              <  2:10090782/150‑1 (MQ=255)

                                                                                       gAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGc                                                           >  2:3835748/1‑150 (MQ=255)

                                                                                         ggTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGccc                                                         >  1:5030335/1‑150 (MQ=255)

                                                                                          gTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCt                                                        <  2:4244416/150‑1 (MQ=255)

                                                                                            tCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCttt                                                      <  1:4622281/150‑1 (MQ=255)

                                                                                             ccGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTta                                                     >  2:6480439/1‑150 (MQ=255)

                                                                                              cGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAATTCAAGGTTGATTCAGCCCTTtaa                                                    <  2:6850991/150‑1 (MQ=255)

                                                                                                cTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGACGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagt                                                  >  2:10309280/1‑150 (MQ=255)

                                                                                                  gggTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                                                <  1:7321744/150‑1 (MQ=255)

                                                                                                   ggTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaag                                               <  2:6704332/150‑1 (MQ=255)

                                                                                                     tGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAagag                                             <  1:6373359/150‑1 (MQ=255)

                                                                                                       agaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGt                                           >  2:9406814/1‑150 (MQ=255)

                                                                                                       agaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGt                                           <  1:10264193/150‑1 (MQ=255)

                                                                                                        gaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGtt                                          <  2:2385046/150‑1 (MQ=255)

                                                                                                          tGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTa                                        <  1:3084421/150‑1 (MQ=255)

                                                                                                            ttCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACt                                      >  1:10840743/1‑150 (MQ=255)

                                                                                                            ttCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACt                                      <  1:2250980/150‑1 (MQ=255)

                                                                                                             tCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTg                                     <  1:8330727/150‑1 (MQ=255)

                                                                                                              cGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGt                                    <  1:6923458/150‑1 (MQ=255)

                                                                                                              cGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGt                                    <  1:8703993/150‑1 (MQ=255)

                                                                                                               ggTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTc                                   <  1:1013914/150‑1 (MQ=255)

                                                                                                                gTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTCCTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCg                                  >  2:3912093/1‑150 (MQ=255)

                                                                                                                gTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCg                                  <  1:7670777/150‑1 (MQ=255)

                                                                                                                 ttATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGt                                 <  1:9444448/150‑1 (MQ=255)

                                                                                                                 ttATGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGt                                 >  2:3520086/1‑150 (MQ=255)

                                                                                                                   aTGCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTgg                               <  2:8382128/150‑1 (MQ=255)

                                                                                                                     gCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCt                             <  1:5449411/150‑1 (MQ=255)

                                                                                                                     gCGACCGACGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCt                             >  2:8859036/1‑150 (MQ=255)

                                                                                                                      cgaccgacGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTa                            >  1:589995/1‑150 (MQ=255)

                                                                                                                          cgacGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGa                        >  2:9290056/1‑150 (MQ=255)

                                                                                                                           gacGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaa                       >  2:5037209/1‑150 (MQ=255)

                                                                                                                           gacGTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaa                       >  1:10370368/1‑150 (MQ=255)

                                                                                                                              gTGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTACGAGGTTTACTGTCGTGGCTAAGGaaaaa                    <  2:8541206/150‑1 (MQ=255)

                                                                                                                               tGCGTTCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAAt                   >  1:433475/1‑150 (MQ=255)

                                                                                                                                   ttCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGa               >  2:5234210/1‑150 (MQ=255)

                                                                                                                                   ttCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGa               >  1:171341/1‑150 (MQ=255)

                                                                                                                                    tCCATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCTAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGaa              <  2:7616077/150‑1 (MQ=255)

                                                                                                                                     ccATGTCCCAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAAc             >  1:5094197/1‑150 (MQ=255)

                                                                                                                                           cccAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACa       <  1:9114676/150‑1 (MQ=255)

                                                                                                                                             cAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACaaa     <  2:3003263/150‑1 (MQ=255)

                                                                                                                                             cAGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACaaa     <  2:5710548/150‑1 (MQ=255)

                                                                                                                                              aGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAAAc    <  1:6667540/150‑1 (MQ=255)

                                                                                                                                              aGGGTTGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAAAc    <  1:5338668/150‑1 (MQ=255)

ATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAAACCG  >  NC_008463/757555‑757848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`