Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 982,940 A→G F293F (TTT→TTC)  PA14_RS04570 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463982,9400AG99.8% 2008.9 / NA 517F293F (TTT→TTC) PA14_RS04570ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (246/270);  minor base T (0/1);  total (246/271)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 522 total aligned reads displayed.

CTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAG  >  NC_008463/982793‑983086
                                                                                                                                                   |                                                                                                                                                  
 tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAAc                                                                                                                                                 <  1:3616060/150‑1 (MQ=255)
   ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAg                                                                                                                                               >  1:10906969/1‑150 (MQ=255)
      cagcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGc                                                                                                                                            >  1:9532825/1‑150 (MQ=255)
       agcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGcc                                                                                                                                           <  2:7763186/150‑1 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                        >  2:445456/1‑150 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                        <  1:7189683/150‑1 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                        <  2:6714289/150‑1 (MQ=255)
          agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                        >  2:1619301/1‑150 (MQ=255)
               gCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggc                                                                                                                                   <  1:4578199/150‑1 (MQ=255)
                     gATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGcc                                                                                                                             <  2:10186043/150‑1 (MQ=255)
                       tgtgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGCTCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCa                                                                                                                           <  1:6877225/150‑1 (MQ=255)
                        gtgCCAGGGGCGCTCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCCTGCCGATCAGGACGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCAt                                                                                                                          <  2:2463980/150‑1 (MQ=255)
                         tgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATc                                                                                                                         <  2:1308019/150‑1 (MQ=255)
                         tgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATc                                                                                                                         <  2:7408452/150‑1 (MQ=255)
                          gCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGACCATCa                                                                                                                        >  2:3373173/1‑150 (MQ=255)
                           ccAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGGGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAg                                                                                                                       >  1:2142239/1‑150 (MQ=255)
                             aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGc                                                                                                                     >  2:3853393/1‑150 (MQ=255)
                             aGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGc                                                                                                                     >  2:10595647/1‑150 (MQ=255)
                              ggggCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGcc                                                                                                                    >  1:8291071/1‑150 (MQ=255)
                                  cgcgcgcCCACCGCGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGCGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcac                                                                                                                <  2:10033405/150‑1 (MQ=255)
                                  cgcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcac                                                                                                                <  2:7238595/150‑1 (MQ=255)
                                   gcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcaca                                                                                                               <  1:11153787/150‑1 (MQ=255)
                                    cgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAg                                                                                                              >  1:405861/1‑150 (MQ=255)
                                    cgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAg                                                                                                              <  1:1505921/150‑1 (MQ=255)
                                     gcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGt                                                                                                             <  2:1225236/150‑1 (MQ=255)
                                       gcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTc                                                                                                           <  2:7505101/150‑1 (MQ=255)
                                        cccACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTcc                                                                                                          >  1:5682735/1‑150 (MQ=255)
                                            ccGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCa                                                                                                      >  1:493636/1‑150 (MQ=255)
                                              gAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAgg                                                                                                    <  1:5617139/150‑1 (MQ=255)
                                              gAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAgg                                                                                                    >  1:1298228/1‑150 (MQ=255)
                                               aGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAggg                                                                                                   <  2:4659810/150‑1 (MQ=255)
                                                    aGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGaca                                                                                              >  1:9702612/1‑150 (MQ=255)
                                                    aGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGaca                                                                                              >  1:7681549/1‑150 (MQ=255)
                                                          gCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                        <  2:6923132/150‑1 (MQ=255)
                                                          gCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                        >  1:2248873/1‑150 (MQ=255)
                                                            cATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCGTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGggag                                                                                      <  2:10953283/150‑1 (MQ=255)
                                                                tcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCCGGCCGGTGCGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                  <  1:1693830/150‑1 (MQ=255)
                                                                tcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                  <  1:2826935/150‑1 (MQ=255)
                                                                    ggcggcGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCa                                                                              <  1:1009268/150‑1 (MQ=255)
                                                                    ggcggcGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCa                                                                              <  1:2537254/150‑1 (MQ=255)
                                                                    ggcggcGCTCGTTGAGGCTGGTGAGGAGCGCCGTGAGCATGCCGCTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCa                                                                              <  2:6610556/150‑1 (MQ=255)
                                                                     gcggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCAt                                                                             <  2:22298/150‑1 (MQ=255)
                                                                         cgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                         <  1:6397376/150‑1 (MQ=255)
                                                                            tCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                      >  1:3055893/1‑150 (MQ=255)
                                                                             cGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGtt                                                                     >  1:7879263/1‑150 (MQ=255)
                                                                              gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTc                                                                    >  2:2969738/1‑150 (MQ=255)
                                                                                     cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTGCGCCGGGa                                                             >  2:3613824/1‑150 (MQ=255)
                                                                                      tGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                            >  2:5409216/1‑150 (MQ=255)
                                                                                      tGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGAGCGCCATCAGCGGTTCGCCGGGAt                                                            >  2:8115566/1‑150 (MQ=255)
                                                                                       ggTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATa                                                           <  1:6926161/150‑1 (MQ=255)
                                                                                         tGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGt                                                         <  2:9613115/150‑1 (MQ=255)
                                                                                         tGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCGTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGt                                                         <  2:6122233/150‑1 (MQ=255)
                                                                                          gAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTc                                                        <  1:6180346/150‑1 (MQ=255)
                                                                                            ggATCGCCGTGAGCCTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGt                                                      <  2:2041055/150‑1 (MQ=255)
                                                                                            ggATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGt                                                      <  2:6360039/150‑1 (MQ=255)
                                                                                               tCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGa                                                   >  2:9108176/1‑150 (MQ=255)
                                                                                               tCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGa                                                   <  2:5212681/150‑1 (MQ=255)
                                                                                                  ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                <  1:5297933/150‑1 (MQ=255)
                                                                                                  ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                >  2:11044339/1‑150 (MQ=255)
                                                                                                   cGTGAGCATGCCGATCAGGCCGGTGAGGCCGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGATCCTGCAGGGAGACACCGGGGAGGATCGCCATCAGCGTTTCGCCGGGATAGTCGTTGACCTc                                               >  2:9763361/1‑150 (MQ=255)
                                                                                                   cGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTc                                               <  2:5772545/150‑1 (MQ=255)
                                                                                                     tGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgc                                             <  1:9040690/150‑1 (MQ=255)
                                                                                                      gAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgcg                                            >  1:8493708/1‑150 (MQ=255)
                                                                                                          aTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCCTCAGCGGGTCGCCGGGATAGTCGTTGACCTCGCGTTGc                                        >  2:5504623/1‑150 (MQ=255)
                                                                                                          aTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGc                                        <  1:9383446/150‑1 (MQ=255)
                                                                                                          aTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGc                                        <  2:3127211/150‑1 (MQ=255)
                                                                                                             ccGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAgg                                     <  1:322671/150‑1 (MQ=255)
                                                                                                             ccGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAgg                                     >  1:8531492/1‑150 (MQ=255)
                                                                                                              cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAggg                                    <  1:7467154/150‑1 (MQ=255)
                                                                                                               gATCTGGCCGGTGAGGTCGACGTACAGCGAGACCACGTCCTGTGCCTTCTCGGCGGTGCCCATCAGGCCCCTCAGTTCGTGCAGGGCGTCTCCGGGGAGGATCGCCATCTGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                   <  2:1859680/150‑1 (MQ=255)
                                                                                                                aTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCg                                  >  1:2905359/1‑150 (MQ=255)
                                                                                                                aTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCg                                  >  2:10254531/1‑150 (MQ=255)
                                                                                                                  cAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGaa                                >  1:4172410/1‑150 (MQ=255)
                                                                                                                   aGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAg                               >  1:10408268/1‑150 (MQ=255)
                                                                                                                    ggCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAgg                              <  2:10821600/150‑1 (MQ=255)
                                                                                                                     gCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                             <  2:9593964/150‑1 (MQ=255)
                                                                                                                     gCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                             <  1:645578/150‑1 (MQ=255)
                                                                                                                       cGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCg                           <  1:4843444/150‑1 (MQ=255)
                                                                                                                        ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGCTGGCCATCCGCGGGTCGCCGGGATAGTCGTTGACCTCGCGTGGCAGGGCGAAGGTCGc                          >  2:8233602/1‑150 (MQ=255)
                                                                                                                        ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGc                          >  1:8769022/1‑150 (MQ=255)
                                                                                                                         gTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGCTAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGcc                         >  2:4795248/1‑150 (MQ=255)
                                                                                                                          tGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                        >  1:5868807/1‑150 (MQ=255)
                                                                                                                             ggACGACGCACTGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGTCACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                     <  2:8423068/150‑1 (MQ=255)
                                                                                                                             ggACGACGAACAGCGAGACCACGAACACTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGTTCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                     <  2:3432294/150‑1 (MQ=255)
                                                                                                                              gacgacGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCtt                    <  1:8086018/150‑1 (MQ=255)
                                                                                                                                cgacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGc                  <  2:1407104/150‑1 (MQ=255)
                                                                                                                                 gacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGct                 <  2:10433301/150‑1 (MQ=255)
                                                                                                                                      aCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTct            >  1:5498320/1‑149 (MQ=255)
                                                                                                                                      aCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGATGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCGCTTCa            >  1:10567740/1‑150 (MQ=255)
                                                                                                                                       cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg           <  1:3304090/150‑1 (MQ=255)
                                                                                                                                       cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg           <  2:4869343/150‑1 (MQ=255)
                                                                                                                                        aGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAgg          >  2:709216/1‑150 (MQ=255)
                                                                                                                                        aGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAgg          >  2:9413109/1‑150 (MQ=255)
                                                                                                                                         gCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGc         >  1:8198951/1‑150 (MQ=255)
                                                                                                                                           gagaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg       >  1:4763340/1‑150 (MQ=255)
                                                                                                                                            agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCgt      >  2:4028853/1‑149 (MQ=255)
                                                                                                                                            agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGa      <  2:8647668/150‑1 (MQ=255)
                                                                                                                                            agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGa      <  1:8897718/150‑1 (MQ=255)
                                                                                                                                             gaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAc     >  1:9468116/1‑150 (MQ=255)
                                                                                                                                              aCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAcc    <  1:6694117/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAG  >  NC_008463/982793‑983086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑