Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,880,8720CG99.8% 2252.9 / NA 576A502G (GCC→GGC) PA14_RS08740DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (289/286);  minor base T (1/0);  total (290/286)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 580 total aligned reads displayed.

TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018
                                                                                                                                                   |                                                                                                                                                  
 ccGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgc                                                                                                                                                 <  1:7518403/150‑1 (MQ=255)
   gcgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                               <  1:10303841/150‑1 (MQ=255)
       cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCt                                                                                                                                           >  2:4235379/1‑150 (MQ=255)
        gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                          >  2:5028473/1‑150 (MQ=255)
         aGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                         >  2:10195522/1‑150 (MQ=255)
          gCCCTCCGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAc                                                                                                                                        <  1:9467230/150‑1 (MQ=255)
          gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAc                                                                                                                                        <  1:5339340/150‑1 (MQ=255)
           cccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                       >  2:7272332/1‑150 (MQ=255)
                cGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCt                                                                                                                                  <  1:2783374/150‑1 (MQ=255)
                cGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCAGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCt                                                                                                                                  <  1:8369532/150‑1 (MQ=255)
                  ccGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGa                                                                                                                                >  1:11184147/1‑150 (MQ=255)
                  ccGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGa                                                                                                                                >  1:2639296/1‑150 (MQ=255)
                   cGGGCTGGAGCGCGAGCGCTTCGACGAAGACCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAc                                                                                                                               <  1:5292519/150‑1 (MQ=255)
                     ggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCt                                                                                                                             >  1:7741789/1‑150 (MQ=255)
                     ggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCt                                                                                                                             <  2:5336377/150‑1 (MQ=255)
                     ggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCt                                                                                                                             >  1:4016888/1‑150 (MQ=255)
                      gCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTc                                                                                                                            <  1:6573210/150‑1 (MQ=255)
                       cTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcg                                                                                                                           >  2:3741109/1‑150 (MQ=255)
                         ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                         >  2:9977228/1‑150 (MQ=255)
                             cgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCCCTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCTGCTGACCGCGCTGACCTcgggcggg                                                                                                                     <  1:636719/150‑1 (MQ=255)
                               cgagcgcGTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGGGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGAc                                                                                                                   >  1:5734989/1‑150 (MQ=255)
                                 agcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCa                                                                                                                 >  2:4442085/1‑150 (MQ=255)
                                 agcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCa                                                                                                                 <  1:1780531/150‑1 (MQ=255)
                                 agcgcTTCGACGAAGTCCTGCGGATGCACGCCGAGGGCTACCACTCACGACTCGGCGTGCGCGGCGCCTATCTCCAACGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCa                                                                                                                 >  2:6446730/1‑150 (MQ=255)
                                  gcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAt                                                                                                                <  2:7608539/150‑1 (MQ=255)
                                    gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                              <  1:3417580/150‑1 (MQ=255)
                                     cTTCGCCGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTAGCTCCATCGCGAGGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTcc                                                                                                             <  2:10669017/150‑1 (MQ=255)
                                      ttCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGGGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTccc                                                                                                            <  1:10940148/150‑1 (MQ=255)
                                         gacgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAt                                                                                                         >  1:3046591/1‑150 (MQ=255)
                                          acgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATa                                                                                                        >  1:3916970/1‑150 (MQ=255)
                                           cgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                       >  2:10896451/1‑150 (MQ=255)
                                           cgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                       <  1:294275/150‑1 (MQ=255)
                                           cgaAGTCCTGCGGATGCTCGCAGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCATATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                       <  1:820759/150‑1 (MQ=255)
                                              aGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                    >  2:10109230/1‑150 (MQ=255)
                                              aGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCAGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                    <  2:1107460/150‑1 (MQ=255)
                                                  cTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGAc                                                                                                <  1:1935841/150‑1 (MQ=255)
                                                       gATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAg                                                                                           >  2:4547893/1‑150 (MQ=255)
                                                       gATGCTCGCCGAGGGCTACCACTCACGCCGCGGCGTGCGCGGCGCCTATCTCCATCGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAGACCGGCGACTACAg                                                                                           <  2:6184571/150‑1 (MQ=255)
                                                           cTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtg                                                                                       <  2:2601412/150‑1 (MQ=255)
                                                           cTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtg                                                                                       <  2:9173830/150‑1 (MQ=255)
                                                             cGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgct                                                                                     >  2:10619692/1‑150 (MQ=255)
                                                                cGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTgctgct                                                                                  <  2:3481548/150‑1 (MQ=255)
                                                                cGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTgctgct                                                                                  <  1:5136218/150‑1 (MQ=255)
                                                                  aGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGTCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                <  1:7957668/150‑1 (MQ=255)
                                                                  aGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                >  1:9700692/1‑150 (MQ=255)
                                                                   gggCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGa                                                                               >  1:6212228/1‑150 (MQ=255)
                                                                          cACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAg                                                                        <  2:3976990/150‑1 (MQ=255)
                                                                            cTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAggg                                                                      >  2:4672470/1‑150 (MQ=255)
                                                                               aCGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                   >  1:10704666/1‑150 (MQ=255)
                                                                                  ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgct                                                                >  1:9970224/1‑150 (MQ=255)
                                                                                    tCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtgg                                                              <  2:1490021/150‑1 (MQ=255)
                                                                                     cGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggt                                                             <  1:6908358/150‑1 (MQ=255)
                                                                                          tGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGaa                                                        >  1:8666813/1‑150 (MQ=255)
                                                                                          tGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGaa                                                        >  1:6457551/1‑150 (MQ=255)
                                                                                             gcggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACgg                                                     <  1:8803718/150‑1 (MQ=255)
                                                                                             gcggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACgg                                                     <  2:9870027/150‑1 (MQ=255)
                                                                                              cggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGt                                                    >  1:10007088/1‑150 (MQ=255)
                                                                                              cggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGt                                                    <  1:9814295/150‑1 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  >  2:520879/1‑150 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  >  1:4487169/1‑150 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  >  2:3106993/1‑150 (MQ=255)
                                                                                                   ccTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                               >  1:5904708/1‑150 (MQ=255)
                                                                                                   ccTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                               >  2:22959/1‑150 (MQ=255)
                                                                                                    cTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGa                                              <  1:7860844/150‑1 (MQ=255)
                                                                                                       tctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGa                                           <  1:4217807/150‑1 (MQ=255)
                                                                                                         tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACt                                         <  2:7941513/150‑1 (MQ=255)
                                                                                                         tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACt                                         >  2:847676/1‑150 (MQ=255)
                                                                                                          ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGACtt                                        >  1:7294383/1‑150 (MQ=255)
                                                                                                             tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                     >  2:8683705/1‑150 (MQ=255)
                                                                                                             tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                     >  2:1625998/1‑150 (MQ=255)
                                                                                                               gcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                   >  2:35848/1‑150 (MQ=255)
                                                                                                               gcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                   >  2:5001717/1‑150 (MQ=255)
                                                                                                                 gATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTg                                 >  2:8018917/1‑150 (MQ=255)
                                                                                                                  ctGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTgg                                <  1:5024938/149‑1 (MQ=255)
                                                                                                                   tGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGga                               >  2:4891462/1‑150 (MQ=255)
                                                                                                                     cgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                             <  2:9576341/150‑1 (MQ=255)
                                                                                                                      gcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                            <  1:3212730/150‑1 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGc                           >  2:9328950/1‑150 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGc                           >  2:7688900/1‑150 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACCGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         <  2:972678/150‑1 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         <  2:5561542/150‑1 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         >  1:8117179/1‑150 (MQ=255)
                                                                                                                             ggCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGcc                     <  1:915410/150‑1 (MQ=255)
                                                                                                                             ggCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGcc                     <  2:3282278/150‑1 (MQ=255)
                                                                                                                              gCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCg                    >  2:1208987/1‑150 (MQ=255)
                                                                                                                                 tGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCg                 <  1:1530156/150‑1 (MQ=255)
                                                                                                                                 tGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCg                 <  2:40781/150‑1 (MQ=255)
                                                                                                                                  gCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGa                <  2:7064066/150‑1 (MQ=255)
                                                                                                                                   cTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGAc               >  2:5878673/1‑150 (MQ=255)
                                                                                                                                       gTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtgt           <  1:10154541/150‑1 (MQ=255)
                                                                                                                                        tGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGtt          >  2:7223138/1‑150 (MQ=255)
                                                                                                                                          gccgccGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTcc        >  1:1049955/1‑150 (MQ=255)
                                                                                                                                          gccgccGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTcc        <  2:193614/150‑1 (MQ=255)
                                                                                                                                            cgccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCaa      <  2:8375213/150‑1 (MQ=255)
                                                                                                                                             gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAAc     <  1:9620748/150‑1 (MQ=255)
                                                                                                                                             gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAAc     >  1:2128806/1‑150 (MQ=255)
                                                                                                                                               cgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg   >  1:4894828/1‑150 (MQ=255)
                                                                                                                                               cgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg   <  2:6781498/150‑1 (MQ=255)
                                                                                                                                                gTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTgg  <  2:10353637/150‑1 (MQ=255)
                                                                                                                                                gTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTgg  >  1:3658571/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑