Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,545,609 T→C I509I (ATT→ATC)  tssI → type VI secretion system tip protein TssI/VgrG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,545,6090TC98.9% 172.3 / NA 93I509I (ATT→ATC) tssItype VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (41/51);  minor base A (1/0);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAC  >  NC_008463/2545462‑2545755
                                                                                                                                                   |                                                                                                                                                  
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  >  1:5382074/1‑150 (MQ=17)
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  <  2:8379200/150‑1 (MQ=17)
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  <  2:7636188/150‑1 (MQ=17)
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  <  1:9662855/150‑1 (MQ=17)
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  >  1:2534816/1‑150 (MQ=17)
cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  <  1:6079742/150‑1 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 >  1:9553474/1‑150 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 >  1:4787106/1‑150 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 >  1:4779807/1‑150 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 <  1:6663409/150‑1 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 <  1:3677900/150‑1 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 <  1:2801726/150‑1 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 <  1:2636519/150‑1 (MQ=17)
 tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 >  2:9515731/1‑150 (MQ=17)
  aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                >  1:1741688/1‑150 (MQ=17)
  aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                <  1:5613411/150‑1 (MQ=17)
  aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                <  1:528990/150‑1 (MQ=17)
   cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  1:9846118/1‑150 (MQ=17)
   cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  1:9846208/1‑150 (MQ=17)
   cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  2:6543703/1‑150 (MQ=17)
   cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  2:1916588/1‑150 (MQ=17)
   cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  2:1937731/1‑150 (MQ=17)
    gAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGCCATCGAGGtc                                                                                                                                              >  2:9517229/1‑149 (MQ=14)
    gAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtc                                                                                                                                              >  2:1572274/1‑149 (MQ=14)
    gAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtc                                                                                                                                              <  2:1579952/150‑2 (MQ=14)
     aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             <  2:10087422/150‑3 (MQ=11)
     aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  1:9956630/1‑148 (MQ=11)
                                                                                                                                 cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 <  2:6187377/150‑1 (MQ=11)
                                                                                                                                 cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 <  2:6187419/150‑1 (MQ=11)
                                                                                                                                 cGAGAGGAACCAGGACATCGAGGACGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 >  1:4892749/1‑150 (MQ=11)
                                                                                                                                  gagaGGAACCAGGACATCGAGGTCGAGACCGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACACCGAGACCATCACCGTGCATGcc                <  2:5719929/150‑1 (MQ=11)
                                                                                                                                  gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  1:7316788/150‑1 (MQ=11)
                                                                                                                                  gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  1:7316848/150‑1 (MQ=11)
                                                                                                                                  gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  1:2908399/150‑1 (MQ=11)
                                                                                                                                   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  1:8454139/1‑150 (MQ=11)
                                                                                                                                   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  1:4006751/1‑150 (MQ=11)
                                                                                                                                   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               <  1:6621308/150‑1 (MQ=11)
                                                                                                                                   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  2:5269995/1‑150 (MQ=11)
                                                                                                                                   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGACGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  2:3583496/1‑150 (MQ=11)
                                                                                                                                   agagGAACCAGGACATAGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  2:3657245/1‑150 (MQ=11)
                                                                                                                                    gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa              <  1:6422295/150‑1 (MQ=11)
                                                                                                                                    gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa              <  1:5614232/150‑1 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGTGACCATCACCGTGCATGCCAAc             <  2:6268458/150‑1 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             >  2:2765752/1‑150 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  2:5382074/150‑1 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             >  2:7521009/1‑150 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  2:9907929/150‑1 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             >  1:4228120/1‑150 (MQ=11)
                                                                                                                                     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  1:1573972/150‑1 (MQ=11)
                                                                                                                                      ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            >  1:5308132/1‑150 (MQ=11)
                                                                                                                                      ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            >  1:10930179/1‑150 (MQ=11)
                                                                                                                                       gAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg           >  1:5696724/1‑150 (MQ=11)
                                                                                                                                        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  1:1078218/150‑1 (MQ=11)
                                                                                                                                        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  2:603011/150‑1 (MQ=11)
                                                                                                                                        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  2:600465/150‑1 (MQ=11)
                                                                                                                                        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  2:1886145/150‑1 (MQ=11)
                                                                                                                                        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          >  1:4930786/1‑150 (MQ=11)
                                                                                                                                         aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca         >  1:9079477/1‑150 (MQ=11)
                                                                                                                                         aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca         <  1:5298655/150‑1 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACACCGAGACCATCACCGTGCATGCCAACCgcag        <  2:8289641/150‑1 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        >  1:2434121/1‑150 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        <  2:6788096/150‑1 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        >  1:5301793/1‑150 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        >  1:10422410/1‑150 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        <  1:6294663/150‑1 (MQ=11)
                                                                                                                                          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag        >  1:6275393/1‑150 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       <  2:3131095/150‑1 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       <  2:10186801/150‑1 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  2:10535412/1‑150 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  1:426243/1‑150 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       <  2:8735519/150‑1 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  2:9877728/1‑150 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  2:3770342/1‑150 (MQ=11)
                                                                                                                                           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  2:9877783/1‑150 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagcc      >  2:10218201/1‑149 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      <  1:7575419/150‑1 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      <  1:4627148/150‑1 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      <  1:8623632/150‑1 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      <  2:7835426/150‑1 (MQ=11)
                                                                                                                                            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      <  1:1653056/150‑1 (MQ=11)
                                                                                                                                             ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     >  2:2835122/1‑150 (MQ=11)
                                                                                                                                             ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     <  2:3895428/150‑1 (MQ=11)
                                                                                                                                              gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  1:6873185/150‑1 (MQ=14)
                                                                                                                                              gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  2:1996624/150‑1 (MQ=14)
                                                                                                                                              gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  1:5578324/150‑1 (MQ=14)
                                                                                                                                              gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  1:8670872/150‑1 (MQ=14)
                                                                                                                                               aCATCGAGGTCGAGAACGACGAGCGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  2:9868096/150‑1 (MQ=14)
                                                                                                                                               aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCCTCACCGTGCATGCCAACCGCAGCAAGa   >  2:4472382/1‑150 (MQ=14)
                                                                                                                                               aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  1:6949382/150‑1 (MQ=14)
                                                                                                                                               aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  1:9966472/150‑1 (MQ=14)
                                                                                                                                               aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  2:9251563/150‑1 (MQ=14)
                                                                                                                                                cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc  <  1:4424767/150‑1 (MQ=14)
                                                                                                                                                cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc  >  1:4425877/1‑150 (MQ=14)
                                                                                                                                                   |                                                                                                                                                  
CTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAC  >  NC_008463/2545462‑2545755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑