Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,651,339 T→C F68L (TTC→CTC)  PA14_RS12480 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,651,3390TC99.8% 1714.3 / NA 442F68L (TTC→CTC) PA14_RS12480MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (217/224);  minor base A (1/0);  total (218/224)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 442 total aligned reads displayed.

GTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCG  >  NC_008463/2651192‑2651485
                                                                                                                                                   |                                                                                                                                                  
     gATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAggg                                                                                                                                             <  1:2369441/150‑1 (MQ=255)
      aTCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggc                                                                                                                                            >  2:4435615/1‑150 (MQ=255)
             tCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGa                                                                                                                                     <  2:977587/150‑1 (MQ=255)
              cGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGAt                                                                                                                                    <  1:1036589/150‑1 (MQ=255)
                 gCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATcga                                                                                                                                 <  1:5163045/150‑3 (MQ=255)
                    cACCGGCATCCTGCTGAACACCTACAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCg                                                                                                                              <  2:4392261/150‑1 (MQ=255)
                     aCCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGt                                                                                                                             <  1:5573707/150‑1 (MQ=255)
                       cGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTg                                                                                                                           <  1:4039537/150‑1 (MQ=255)
                       cGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTg                                                                                                                           <  1:6928398/150‑1 (MQ=255)
                        ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgg                                                                                                                          >  2:7553842/1‑150 (MQ=255)
                           aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgc                                                                                                                       >  2:4692447/1‑150 (MQ=255)
                           aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgc                                                                                                                       >  2:6915120/1‑150 (MQ=255)
                                gctgAACACCTTCAGGCTGTTCATCGCAGCGATGAGAGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACACGCCCGATCTAGGGCTGGCTGCTCGACCGTGGGGGcgcgcg                                                                                                                  >  2:7602908/1‑150 (MQ=255)
                                  tgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggc                                                                                                                <  2:1127369/150‑1 (MQ=255)
                                     acacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGATGGCTGATCGACCGTTGGGGCGCGCGGCGGa                                                                                                             >  1:2009413/1‑150 (MQ=255)
                                      cacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGAc                                                                                                            <  2:4834479/150‑1 (MQ=255)
                                       acCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACa                                                                                                           <  2:9940430/150‑1 (MQ=255)
                                         cTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                         <  1:8590542/150‑1 (MQ=255)
                                         cTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                         >  1:10197856/1‑150 (MQ=255)
                                         cTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                         <  1:3152817/150‑1 (MQ=255)
                                             aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTc                                                                                                     >  1:3568921/1‑150 (MQ=255)
                                              gTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                    >  2:3488245/1‑150 (MQ=255)
                                               tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                   >  2:2414216/1‑150 (MQ=255)
                                               tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                   <  1:1128495/150‑1 (MQ=255)
                                                 tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGc                                                                                                 <  2:938054/150‑1 (MQ=255)
                                                 tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGc                                                                                                 >  1:6412372/1‑150 (MQ=255)
                                                  gTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCt                                                                                                >  2:5898938/1‑150 (MQ=255)
                                                   ttCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTg                                                                                               <  1:496641/150‑1 (MQ=255)
                                                   ttCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTg                                                                                               <  1:7332196/150‑1 (MQ=255)
                                                    tCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGa                                                                                              >  2:5243425/1‑150 (MQ=255)
                                                     cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCACCGATGAGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAc                                                                                             <  1:2489375/150‑1 (MQ=255)
                                                            ccGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGcc                                                                                      <  1:9966935/150‑1 (MQ=255)
                                                               aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                   >  1:10040019/1‑150 (MQ=255)
                                                               aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                   >  2:3984231/1‑150 (MQ=255)
                                                               aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                   <  1:6564915/150‑1 (MQ=255)
                                                                tGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGt                                                                                  >  2:3376682/1‑150 (MQ=255)
                                                                   gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgc                                                                               >  1:7468734/1‑150 (MQ=255)
                                                                   gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgc                                                                               >  1:220118/1‑150 (MQ=255)
                                                                   gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgc                                                                               <  1:1789043/150‑1 (MQ=255)
                                                                    cgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgct                                                                              >  1:7908748/1‑150 (MQ=255)
                                                                     gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctg                                                                             <  1:26792/150‑1 (MQ=255)
                                                                     gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctg                                                                             >  1:4660182/1‑150 (MQ=255)
                                                                      ccGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                            >  2:7092350/1‑150 (MQ=255)
                                                                         aGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAAGCTCGGGCTGACAGCGGCCCTGTcgctgccgc                                                                         >  1:5215299/1‑150 (MQ=255)
                                                                            aCGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgctgc                                                                      <  1:4524998/150‑1 (MQ=255)
                                                                             cGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                     >  2:8335214/1‑150 (MQ=255)
                                                                                cTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCgg                                                                  >  1:6048698/1‑150 (MQ=255)
                                                                                 tGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGc                                                                 <  1:2645805/150‑1 (MQ=255)
                                                                                 tGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGc                                                                 >  2:6640798/1‑150 (MQ=255)
                                                                                   ggACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCt                                                               >  1:3099282/1‑150 (MQ=255)
                                                                                    gACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTg                                                              >  2:81353/1‑150 (MQ=255)
                                                                                      ccGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggc                                                            <  2:8710152/150‑1 (MQ=255)
                                                                                        gcgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcgg                                                          >  1:4847833/1‑150 (MQ=255)
                                                                                        gcgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcgg                                                          <  2:287025/150‑1 (MQ=255)
                                                                                             gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                     <  2:649181/150‑1 (MQ=255)
                                                                                              aGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGa                                                    <  1:7436280/150‑1 (MQ=255)
                                                                                                gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAAt                                                  <  1:385745/150‑1 (MQ=255)
                                                                                                 tgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATg                                                 >  2:561461/1‑150 (MQ=255)
                                                                                                   tCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGcc                                               >  1:8025317/1‑150 (MQ=255)
                                                                                                   tCGTTGCGGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCAGCCGCTGCTCGGCCTGGCGGCGGTGAATGcc                                               >  2:9569226/1‑150 (MQ=255)
                                                                                                    cGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCg                                              <  1:9561711/150‑1 (MQ=255)
                                                                                                      ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTc                                            <  2:73579/150‑1 (MQ=255)
                                                                                                      ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTc                                            >  1:4610009/1‑150 (MQ=255)
                                                                                                         cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTgg                                         <  1:8774743/150‑1 (MQ=255)
                                                                                                         cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTgg                                         <  2:610481/150‑1 (MQ=255)
                                                                                                           ggTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCa                                       <  1:2398849/150‑1 (MQ=255)
                                                                                                            gTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCaa                                      <  2:9128817/150‑1 (MQ=255)
                                                                                                            gTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGGTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCaa                                      >  2:7840828/1‑150 (MQ=255)
                                                                                                              gACGCTGTTCGGACTGGCGGTCGCCATCCCCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCGGGCGGCGGTGAATGCCGTCTGGctcct                                    >  2:575903/1‑146 (MQ=255)
                                                                                                              gACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAct                                    >  1:11090414/1‑150 (MQ=255)
                                                                                                              gACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAct                                    >  1:10701684/1‑150 (MQ=255)
                                                                                                                  cTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAc                                >  2:9194445/1‑150 (MQ=255)
                                                                                                                    gTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                              >  2:6714207/1‑150 (MQ=255)
                                                                                                                    gTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                              >  2:8440842/1‑150 (MQ=255)
                                                                                                                    gTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                              <  1:72187/150‑1 (MQ=255)
                                                                                                                      tCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACgcgc                            >  2:10415738/1‑150 (MQ=255)
                                                                                                                       cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                           <  2:1709841/150‑1 (MQ=255)
                                                                                                                        ggACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACACTGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTg                          <  2:5337593/150‑1 (MQ=255)
                                                                                                                         gACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGt                         >  1:3701173/1‑150 (MQ=255)
                                                                                                                         gACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGt                         >  1:1695087/1‑150 (MQ=255)
                                                                                                                         gACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGt                         >  2:1478157/1‑150 (MQ=255)
                                                                                                                            tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCCACTCTACGCGCTGTTcc                      >  2:10631264/1‑150 (MQ=255)
                                                                                                                            tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTcc                      >  1:4296572/1‑150 (MQ=255)
                                                                                                                             ggCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                     >  1:5831852/1‑150 (MQ=255)
                                                                                                                             ggCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                     <  1:9797521/150‑1 (MQ=255)
                                                                                                                                ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                  <  2:2056977/150‑1 (MQ=255)
                                                                                                                                   cGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAt               <  2:153440/150‑1 (MQ=255)
                                                                                                                                   cGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAt               <  2:3649624/150‑1 (MQ=255)
                                                                                                                                    gCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATc              <  1:8878216/150‑1 (MQ=255)
                                                                                                                                     ccATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCg             <  2:2299022/150‑1 (MQ=255)
                                                                                                                                     ccATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGCCGGAGGTGAATGCCGTCTGTCAACTCTACGCGCTGTTCCTGCTGATCg             >  2:10157565/1‑150 (MQ=255)
                                                                                                                                       aTCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc           >  2:6789389/1‑150 (MQ=255)
                                                                                                                                        tCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGcc          >  2:127798/1‑150 (MQ=255)
                                                                                                                                           ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgc       <  1:10395763/150‑1 (MQ=255)
                                                                                                                                           ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgc       <  1:1382409/150‑1 (MQ=255)
                                                                                                                                            cAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct      >  1:8267683/1‑150 (MQ=255)
                                                                                                                                             aGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg     <  2:8038205/150‑1 (MQ=255)
                                                                                                                                             aGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg     <  1:9299088/150‑1 (MQ=255)
                                                                                                                                             aGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg     >  1:8350807/1‑150 (MQ=255)
                                                                                                                                              gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAGGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTgg    <  2:265766/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCG  >  NC_008463/2651192‑2651485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑