BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,673,534	3 bp→CTC	intergenic (+83/+13)	fabI → / ← PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,673,534	0	T	C	99.8%	1650.4 / NA	428	intergenic (+83/+15)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (229/196); minor base A (1/0); total (231/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	3,673,535	0	C	T	96.0%	1776.9 / 35.2	445	intergenic (+84/+14)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (10/8); new base T (231/196); total (241/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01
*	NC_008463	3,673,536	0	T	C	96.2%	1632.7 / 45.1	445	intergenic (+85/+13)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (10/7); new base C (231/196); total (241/204)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

Only 100 of 445 total aligned reads displayed.

TCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCT  >  NC_008463/3673393‑3673679

|||

   tctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCg                                                                                                                                        >  1:7944580/1‑150 (MQ=255)

   tctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCg                                                                                                                                        <  1:9990175/150‑1 (MQ=255)

   tcgaCGTCGATGGCGGCTACAACCCTCCCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCTTGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCg                                                                                                                                        <  1:489029/147‑1 (MQ=255)

        gTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtctc                                                                                                                                   <  2:10864115/150‑1 (MQ=255)

        gTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtcgc                                                                                                                                   >  1:7568365/1‑148 (MQ=255)

         acGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCTAGCGGCCTTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCa                                                                                                                                  <  1:6419971/149‑1 (MQ=255)

           gATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGt                                                                                                                                >  2:536060/1‑150 (MQ=255)

             tGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGAACACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTAt                                                                                                                              <  2:8335583/150‑1 (MQ=255)

                 ggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTtct                                                                                                                          >  1:5833100/1‑150 (MQ=255)

                  gcTTCACCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTtctc                                                                                                                         <  1:2969556/150‑1 (MQ=255)

                   cTTCAACACTCCCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGACTAAACAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCGCAGTATTTCTCg                                                                                                                        <  2:9717676/150‑1 (MQ=255)

                   cTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCg                                                                                                                        >  1:3422305/1‑150 (MQ=255)

                   cTTAAACACTCCCCCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCCCGCGGCATTTTTATTGGGCGTAAAAAACAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCg                                                                                                                        <  1:1918339/150‑1 (MQ=255)

                    ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGa                                                                                                                       >  1:6054823/1‑150 (MQ=255)

                     tCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAATGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                      >  2:9384643/1‑150 (MQ=255)

                     tCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                      >  2:5712270/1‑150 (MQ=255)

                      cAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAGCTCGGTCTCAGTATTTCTCGACt                                                                                                                     >  2:4855854/1‑150 (MQ=255)

                        acacTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                                   <  2:5029976/150‑1 (MQ=255)

                         cccTACCGCCTTGGGCCCGCTGGACGACGACTGACCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCAGGGGGGGAATAAAGAAAAGCCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTcc                                                                                                                  <  1:1136355/148‑1 (MQ=255)

                          acTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCGTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCg                                                                                                                 >  2:7450709/1‑150 (MQ=255)

                           ctcccGCCAGGGCCCCGCGGGACGACGACTGATCACCCCGCTCATTAAAAATGGCCGCTTCGTGGGGCATTTTTATTGGGCGGAATAAACAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGc                                                                                                                <  1:3704408/147‑1 (MQ=255)

                              ccGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCtt                                                                                                             >  1:1488898/1‑150 (MQ=255)

                               cGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTg                                                                                                            <  2:9726384/150‑1 (MQ=255)

                                 ccATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                                          >  1:512446/1‑150 (MQ=255)

                                  cATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAATTGGCCGATTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTcc                                                                                                         <  1:5712438/150‑1 (MQ=255)

                                   aTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCGTGTCCt                                                                                                        >  2:8794481/1‑150 (MQ=255)

                                          cGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTg                                                                                                 <  1:9791109/150‑1 (MQ=255)

                                               gacgacgacTGATCACCCCGCTCATTAAAAATGGCCGCTTCGAGCGGCTTTTTTCAGGGGCGGAATACAGAAAAGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                            <  1:4770431/150‑1 (MQ=255)

                                                   acgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGaa                                                                                        >  2:617679/1‑150 (MQ=255)

                                                    cgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAg                                                                                       >  1:4340328/1‑150 (MQ=255)

                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                                      <  2:8968347/150‑1 (MQ=255)

                                                      acTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggc                                                                                     <  2:6396139/150‑1 (MQ=255)

                                                       cTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcg                                                                                    <  2:3633704/150‑1 (MQ=255)

                                                        tGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcgg                                                                                   >  2:10523668/1‑150 (MQ=255)

                                                           tCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGa                                                                                >  2:8003298/1‑150 (MQ=255)

                                                             aCCCCGCTCATGAAAAATGGCCGCTTCGCGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGCCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAg                                                                              <  1:2080876/150‑1 (MQ=255)

                                                               cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTc                                                                            <  1:8579743/150‑1 (MQ=255)

                                                                ccGCTCATTAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCt                                                                           <  1:10986018/150‑1 (MQ=255)

                                                                 cGCTCATGATAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTATGCGGCCGTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCGTGTCCTGCCACTGACGGCGGAAGGCGGCGAAGTCTg                                                                          >  2:8635498/1‑150 (MQ=255)

                                                                  gCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGc                                                                         <  2:2601769/150‑1 (MQ=255)

                                                                   cccctGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAACTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGcc                                                                        <  1:5544941/146‑1 (MQ=255)

                                                                    tCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCt                                                                       >  1:4205046/1‑150 (MQ=255)

                                                                        gAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTcc                                                                   >  1:4546831/1‑150 (MQ=255)

                                                                        gAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGCCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTcc                                                                   <  1:6760437/150‑1 (MQ=255)

                                                                           aaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCt                                                                >  2:9245478/1‑150 (MQ=255)

                                                                            aaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                               >  2:9203171/1‑150 (MQ=255)

                                                                            aaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                               >  1:459709/1‑150 (MQ=255)

                                                                             aTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgc                                                              <  1:2171001/150‑1 (MQ=255)

                                                                                     ttCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                                      <  1:8858151/150‑1 (MQ=255)

                                                                                     ttCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTGTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                                      >  2:3473992/1‑150 (MQ=255)

                                                                                       cGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                                    >  2:1605284/1‑150 (MQ=255)

                                                                                        gAGCGGCCTTTTTCATGGGCGGAATAAATAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAgg                                                   >  1:8741915/1‑150 (MQ=255)

                                                                                        gAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAgg                                                   >  2:908049/1‑150 (MQ=255)

                                                                                         aGCGGCCTTTTTCATGGGCGGCATAAAGACAAGGCCGCGTAAGCGGCCTTTTCTC‑AGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                  <  1:10106049/150‑1 (MQ=255)

                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                  >  1:6477196/1‑150 (MQ=255)

                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                  <  1:736778/150‑1 (MQ=255)

                                                                                          gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                 <  2:7191989/150‑1 (MQ=255)

                                                                                          gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                 >  1:3316692/1‑150 (MQ=255)

                                                                                           cGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAg                                                <  2:1301371/150‑1 (MQ=255)

                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                               >  1:11160552/1‑150 (MQ=255)

                                                                                              ccTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcgg                                             >  1:2561762/1‑150 (MQ=255)

                                                                                              ccTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACGTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcgg                                             <  2:2275297/150‑1 (MQ=255)

                                                                                               cTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggc                                            <  1:7166687/150‑1 (MQ=255)

                                                                                               cTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggc                                            >  1:2415606/1‑150 (MQ=255)

                                                                                                 ttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGcggcgg                                          <  1:8059609/150‑1 (MQ=255)

                                                                                                 ttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGcggcgg                                          >  2:6619802/1‑150 (MQ=255)

                                                                                                   ttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTa                                        >  1:6993125/1‑150 (MQ=255)

                                                                                                   ttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTa                                        >  1:1241690/1‑150 (MQ=255)

                                                                                                     cATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACa                                      >  2:7121062/1‑150 (MQ=255)

                                                                                                     cATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACa                                      >  2:5821690/1‑150 (MQ=255)

                                                                                                       tGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATg                                    <  1:1633935/150‑1 (MQ=255)

                                                                                                        gggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGACGCCAGGAAGCGGCGGTACATgg                                   >  1:1379031/1‑150 (MQ=255)

                                                                                                         ggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGc                                  >  2:2928722/1‑150 (MQ=255)

                                                                                                           cGGAATAAAGAAAAGGCCTCGTAAGCGGCCTTTTCTC‑TGCATAGCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGTAGTCTGCCTGTCAGCTGGGCGAAGCCAGGAAGCGGCGGTACATGGCCt                                <  2:7889859/150‑1 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               <  1:3176258/150‑1 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               >  1:7298343/1‑150 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               <  2:9551631/150‑1 (MQ=255)

                                                                                                               aTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                            >  1:8121015/1‑150 (MQ=255)

                                                                                                                tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcg                           >  2:4686464/1‑150 (MQ=255)

                                                                                                                 aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                          <  1:10018862/150‑1 (MQ=255)

                                                                                                                   aGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcg                        <  1:10832554/150‑1 (MQ=255)

                                                                                                                   aGAAAAGGCCGCGTAAGCGGCCATTTCTC‑TGCATCTCGGTCACAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcg                        <  2:6297136/150‑1 (MQ=255)

                                                                                                                     aaaaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgtc                      >  1:9402916/1‑150 (MQ=255)

                                                                                                                        cggCCGCTTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGga                   <  1:7698100/149‑1 (MQ=255)

                                                                                                                        aGGCCGCGTAAGCGGCCTTTTCTC‑TGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGga                   <  2:4016469/150‑1 (MQ=255)

                                                                                                                            cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa               <  2:1735794/150‑1 (MQ=255)

                                                                                                                             gCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg              >  2:330934/1‑150 (MQ=255)

                                                                                                                             gCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg              >  2:3888435/1‑150 (MQ=255)

                                                                                                                              cgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGc             <  1:9623384/149‑1 (MQ=255)

                                                                                                                              cgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTGGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGc             >  2:2963893/2‑150 (MQ=255)

                                                                                                                               gTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcc            <  2:7537045/150‑1 (MQ=255)

                                                                                                                                tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct           >  2:2028135/1‑150 (MQ=255)

                                                                                                                                    cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCctcctc       <  1:4009114/150‑1 (MQ=255)

                                                                                                                                    cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCctcctc       <  1:10193540/150‑1 (MQ=255)

                                                                                                                                     ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc      >  2:1113305/1‑150 (MQ=255)

                                                                                                                                     ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc      <  2:5281391/150‑1 (MQ=255)

                                                                                                                                      gCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCg     >  1:9211118/1‑150 (MQ=255)

                                                                                                                                      gCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCg     >  2:690457/1‑150 (MQ=255)

                                                                                                                                        ccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCgg    >  1:6313379/3‑150 (MQ=255)

                                                                                                                                         cttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGc   >  1:8331192/2‑150 (MQ=255)

|||

TCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCT  >  NC_008463/3673393‑3673679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`