BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,103,224	2 bp→CA	coding (818‑819/1185 nt)	ftsZ ←	cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,103,224	0	A	C	100.0%	1855.5 / NA	478	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (282/196); total (282/196)
*	NC_008463	5,103,225	0	G	A	99.8%	1971.4 / NA	478	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (281/195); minor base C (0/1); total (282/196)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 478 total aligned reads displayed.

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATC  >  NC_008463/5103080‑5103368

||

ctcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGa                                                                                                                                             <  2:4253894/150‑1 (MQ=255)

 tcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGAc                                                                                                                                            <  1:7127058/150‑1 (MQ=255)

 tcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGAc                                                                                                                                            <  1:8255190/150‑1 (MQ=255)

 tcCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGAc                                                                                                                                            <  2:5378493/150‑1 (MQ=255)

  ccAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACa                                                                                                                                           <  1:4800493/150‑1 (MQ=255)

    aGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAgg                                                                                                                                         <  1:6476439/150‑1 (MQ=255)

          gcgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGa                                                                                                                                   <  2:11050806/150‑1 (MQ=255)

             cccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                >  1:491840/1‑150 (MQ=255)

             cccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                >  1:9810625/1‑150 (MQ=255)

             cccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                >  2:4075805/1‑150 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACCGGTCCGGACCCGCGGTgt                                                                                                                         >  2:9210424/1‑149 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGa                                                                                                                         >  1:3975809/1‑150 (MQ=255)

                     ggTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGAt                                                                                                                        >  1:7817831/1‑150 (MQ=255)

                        ggCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGtt                                                                                                                     >  2:4942085/1‑150 (MQ=255)

                        ggCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGtt                                                                                                                     <  1:7270964/150‑1 (MQ=255)

                         gCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTc                                                                                                                    <  1:1591482/150‑1 (MQ=255)

                         gCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTc                                                                                                                    <  1:7540662/150‑1 (MQ=255)

                         gCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACATTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTc                                                                                                                    >  2:2552118/1‑150 (MQ=255)

                          cGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCa                                                                                                                   <  1:2188988/150‑1 (MQ=255)

                          cGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCa                                                                                                                   >  2:7803303/1‑150 (MQ=255)

                          cGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGGTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCa                                                                                                                   >  2:2218589/1‑150 (MQ=255)

                               aCGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCagg                                                                                                              >  1:8610840/1‑148 (MQ=255)

                                     aCGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcg                                                                                                        <  2:9940664/150‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGGCCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       <  1:6036783/150‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       <  2:1920889/150‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       <  1:10112894/150‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       >  1:619595/1‑150 (MQ=255)

                                         gCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgcg                                                                                                    >  1:1218371/1‑150 (MQ=255)

                                             cTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGcc                                                                                                >  1:3708355/1‑150 (MQ=255)

                                              tCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGccc                                                                                               >  1:3248499/1‑150 (MQ=255)

                                                 tCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGc                                                                                            <  2:986846/150‑1 (MQ=255)

                                                   gcgcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAg                                                                                          <  2:2395868/150‑1 (MQ=255)

                                                     gcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGt                                                                                        >  1:2432943/1‑150 (MQ=255)

                                                           ccGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGt                                                                                  <  2:2256173/150‑1 (MQ=255)

                                                               gTCGATCACGGTGCCCACCTTCACAGTGGCGAGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGAAGCCGCGCGCGCCCTGCAGGTTGACGtcttc                                                                              <  2:9598880/150‑3 (MQ=255)

                                                                   aTCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagc                                                                          >  2:8911654/1‑150 (MQ=255)

                                                                   aTCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagc                                                                          >  1:10661210/1‑150 (MQ=255)

                                                                   aTCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCcagc                                                                          >  2:521752/1‑150 (MQ=255)

                                                                      aCGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCagcagc                                                                       >  2:2823397/1‑150 (MQ=255)

                                                                       cGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCg                                                                      <  1:5597340/150‑1 (MQ=255)

                                                                       cGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCg                                                                      >  2:3802681/1‑150 (MQ=255)

                                                                          tGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCtggt                                                                   >  2:6945775/1‑149 (MQ=255)

                                                                           gCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgggtt                                                                  >  1:10902161/1‑148 (MQ=255)

                                                                            cccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                 >  2:5101158/1‑150 (MQ=255)

                                                                             ccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGc                                                                >  1:2241811/1‑150 (MQ=255)

                                                                             ccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGc                                                                >  2:10224620/1‑150 (MQ=255)

                                                                              cACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCg                                                               >  2:2870415/1‑150 (MQ=255)

                                                                               aCCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCgg                                                              >  2:10897899/1‑150 (MQ=255)

                                                                                 cTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAt                                                            >  2:947508/1‑150 (MQ=255)

                                                                                   tCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTg                                                          >  1:5532567/1‑150 (MQ=255)

                                                                                   tCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTg                                                          <  1:6774289/150‑1 (MQ=255)

                                                                                    cacaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGCCCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgc                                                         <  2:6212339/150‑1 (MQ=255)

                                                                                    cacaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgc                                                         <  1:5124819/150‑1 (MQ=255)

                                                                                     acaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgcc                                                        >  2:9732558/1‑150 (MQ=255)

                                                                                     acaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgcc                                                        <  1:1983652/150‑1 (MQ=255)

                                                                                       aGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgccgc                                                      <  1:3811179/150‑1 (MQ=255)

                                                                                        gTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgccgcc                                                     <  2:609478/150‑1 (MQ=255)

                                                                                        gTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgccgcc                                                     >  2:7384694/1‑150 (MQ=255)

                                                                                           gCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCg                                                  >  2:7147885/1‑150 (MQ=255)

                                                                                           gCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCg                                                  >  2:8563740/1‑150 (MQ=255)

                                                                                           gCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCg                                                  >  1:6247965/1‑150 (MQ=255)

                                                                                                  cGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCt                                           <  1:8206672/150‑1 (MQ=255)

                                                                                                  cGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCt                                           >  1:6625949/1‑150 (MQ=255)

                                                                                                   ggAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          >  2:7717579/1‑150 (MQ=255)

                                                                                                   ggAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          <  2:4608664/150‑1 (MQ=255)

                                                                                                     aaGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgc                                        >  2:8303109/1‑150 (MQ=255)

                                                                                                     aaGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgc                                        <  1:9306171/150‑1 (MQ=255)

                                                                                                     aaGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgc                                        >  2:4458102/1‑150 (MQ=255)

                                                                                                      aGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgcg                                       >  1:10415341/1‑150 (MQ=255)

                                                                                                       gCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCgg                                      >  2:6750252/1‑150 (MQ=255)

                                                                                                         gAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGc                                    >  2:5513423/1‑150 (MQ=255)

                                                                                                           aCTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCAc                                  <  1:8794955/150‑1 (MQ=255)

                                                                                                            cTGTTCGATGATGGTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACg                                 <  2:8986502/150‑1 (MQ=255)

                                                                                                             tGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACgg                                >  2:1566562/1‑150 (MQ=255)

                                                                                                              gTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGt                               <  2:3542531/150‑1 (MQ=255)

                                                                                                              gTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGt                               >  2:1320375/1‑150 (MQ=255)

                                                                                                                tCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTc                             <  1:3400576/150‑1 (MQ=255)

                                                                                                                tCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTc                             <  2:10771788/150‑1 (MQ=255)

                                                                                                                  gatgatGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGGTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCgg                           >  1:2584599/1‑150 (MQ=255)

                                                                                                                   atgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGa                          >  1:9562715/1‑150 (MQ=255)

                                                                                                                   atgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGa                          >  1:4221632/1‑150 (MQ=255)

                                                                                                                      atgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCg                       >  2:5701810/1‑150 (MQ=255)

                                                                                                                      atgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCg                       <  1:9402640/150‑1 (MQ=255)

                                                                                                                       tgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGc                      >  1:10055210/1‑150 (MQ=255)

                                                                                                                         ttGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTg                    >  1:10227630/1‑150 (MQ=255)

                                                                                                                         ttGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTg                    >  1:9141740/1‑150 (MQ=255)

                                                                                                                          tGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTgg                   <  1:7973377/150‑1 (MQ=255)

                                                                                                                              gACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa               >  1:3027634/1‑150 (MQ=255)

                                                                                                                              gACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa               >  2:3134797/1‑150 (MQ=255)

                                                                                                                              gACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa               >  2:10447225/1‑150 (MQ=255)

                                                                                                                               acctCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAg              >  1:2917313/4‑150 (MQ=255)

                                                                                                                               aCATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAg              >  2:8444714/1‑150 (MQ=255)

                                                                                                                               aCATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAg              >  2:6301707/1‑150 (MQ=255)

                                                                                                                                cATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGc             <  2:8030062/150‑1 (MQ=255)

                                                                                                                                    ggAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGt         <  1:8519860/150‑1 (MQ=255)

                                                                                                                                     gAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTa        >  1:4479393/1‑150 (MQ=255)

                                                                                                                                        tACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTAccc     >  1:4671064/1‑150 (MQ=255)

                                                                                                                                         aCTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCca    <  1:6834904/150‑1 (MQ=255)

                                                                                                                                           tCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTCCCcatc  >  2:7456931/1‑150 (MQ=255)

                                                                                                                                           tCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCcatc  >  1:9988722/1‑150 (MQ=255)

||

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATC  >  NC_008463/5103080‑5103368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`