Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,156 3 bp→GCG intergenic (+17/‑42) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1560NG99.8% 1615.5 / NA 435intergenic (+17/‑44)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (198/236);  minor base T (0/1);  total (198/237)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0084631,128,1570NC99.8% 1584.7 / NA 435intergenic (+18/‑43)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (197/236);  minor base A (1/0);  total (198/237)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0084631,128,1580NG99.3% 1578.9 / NA 435intergenic (+19/‑42)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (198/234);  minor base T (0/3);  total (198/237)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304
                                                                                                                                                  |||                                                                                                                                                   
  tGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAc                                                                                                                                                  <  2:2837201/150‑1 (MQ=255)
  tGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAc                                                                                                                                                  >  1:9007621/1‑150 (MQ=255)
   gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAcc                                                                                                                                                 <  2:8827974/150‑2 (MQ=255)
    ggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACATCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGGTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                >  2:2117006/1‑150 (MQ=255)
      ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCggg                                                                                                                                              >  2:2164280/1‑149 (MQ=255)
        tGGACGCGGAGTACCAGAACACCTACACCGCGAGGCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGTAAGTCTGATGTACGCCGGCCCCGTGCGAACCGGGcc                                                                                                                                            >  1:10252529/1‑150 (MQ=255)
           aCGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGGACCGGGCCGGc                                                                                                                                         >  1:9874466/1‑150 (MQ=255)
             gcgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGa                                                                                                                                       <  1:3624756/150‑1 (MQ=255)
               ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGgcgcgc                                                                                                                                     >  2:9089394/1‑150 (MQ=255)
               ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCAGGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                     >  2:7812324/1‑150 (MQ=255)
                  gTACCAGAACACCTACACCGCGAGCCTCACCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                  <  2:7858044/150‑1 (MQ=255)
                     ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACt                                                                                                                               <  1:8058835/150‑1 (MQ=255)
                       aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                             >  2:2931732/1‑150 (MQ=255)
                         aaCACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                           >  2:5042287/1‑150 (MQ=255)
                            acCTCCACCGCGAGCCTCTCCTACACCAACTTCTTCGACGTCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                        <  2:4259032/150‑1 (MQ=255)
                            acCTACACCGCGAGCCTCTCCTACCCCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                        <  1:1825526/150‑1 (MQ=255)
                            acCTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                        <  2:6487006/150‑1 (MQ=255)
                            acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                        >  2:9881737/1‑150 (MQ=255)
                              ctccacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCCCGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGTCATGAACTTCTGCTGTACGCCGGCCCGGTGCGAACCTTGCCGGCGAGCAGGACTTAACGAGga                                                                                                                      <  2:9163280/147‑1 (MQ=255)
                              cTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTCCACCACCGTGTACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                      <  2:4764560/150‑1 (MQ=255)
                              cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                      <  1:6333355/150‑1 (MQ=255)
                              cTACACCGCGAGCCTCTCCTACACAACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCTACTTCGTCGCGATCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                      <  1:3748304/150‑1 (MQ=255)
                               tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                     <  1:1536857/150‑1 (MQ=255)
                                 cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacga                                                                                                                   >  2:5854023/1‑150 (MQ=255)
                                  acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACTATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTACTGATGTACGCCGGCCCGGTGAGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                  >  2:10894133/1‑150 (MQ=255)
                                   ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAACGAGGcagacg                                                                                                                 >  2:410672/1‑150 (MQ=255)
                                     gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACGTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                               <  2:7412575/150‑1 (MQ=255)
                                      cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                              <  1:11092562/150‑1 (MQ=255)
                                          ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                          <  2:3007473/150‑1 (MQ=255)
                                           ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCAATAACGTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                         >  1:3366295/1‑150 (MQ=255)
                                               ccTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑aaaa                                                                                                    <  1:3467028/150‑1 (MQ=255)
                                               ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑aaaa                                                                                                    <  1:9649780/150‑1 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGTCTTCGTCGCGCTCAGCTTCGGCATGTACGTCTGATGTACGCCCGCCCGGTGCGCACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAAG‑AAaac                                                                                                   >  1:4241425/1‑150 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGTACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaac                                                                                                   >  1:2759717/1‑150 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaac                                                                                                   <  1:10667106/150‑1 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaac                                                                                                   >  2:4873305/1‑150 (MQ=255)
                                                 aacacCAACTTCTTCGACGGCAAGTACACCACCGTGGACCATCGCTACTTCGACGCGCTCAGCTTCGGCATGAACTTCTGCTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaaca                                                                                                  <  1:4644514/149‑1 (MQ=255)
                                                  acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaacaa                                                                                                 <  1:8560911/150‑1 (MQ=255)
                                                   cacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGACCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaacaac                                                                                                <  2:5313139/150‑1 (MQ=255)
                                                   cacCAACTTCTTCGACGGAAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACTAGGACGACGAGCGAATG‑AAaacaac                                                                                                <  2:6244046/150‑1 (MQ=255)
                                                        aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGa                                                                                           <  1:10419703/150‑1 (MQ=255)
                                                          ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAtt                                                                                         <  2:6842891/150‑1 (MQ=255)
                                                            cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAttct                                                                                       <  2:8695491/150‑1 (MQ=255)
                                                             ttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAttctt                                                                                      <  2:7983049/150‑1 (MQ=255)
                                                              tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTG‑AAAACAACGAAGAttcttc                                                                                     >  2:1393736/1‑150 (MQ=255)
                                                              tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCAGCTTAGGCAAGAACTTAGGATGTACGCCGGCCCCGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTG‑AAAACAACGAAGAttcttc                                                                                     >  1:2103206/1‑150 (MQ=255)
                                                                gACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGCCTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTg                                                                                   >  2:7567668/1‑150 (MQ=255)
                                                                 aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGAGGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGc                                                                                  <  2:228046/150‑1 (MQ=255)
                                                                  cGGCAAGTACACCACCGTGGACGCTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCa                                                                                 <  1:8775855/150‑1 (MQ=255)
                                                                    gCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATa                                                                               >  2:3523957/1‑150 (MQ=255)
                                                                    gCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATa                                                                               >  2:67653/1‑150 (MQ=255)
                                                                      aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATAcc                                                                             <  1:10011253/150‑1 (MQ=255)
                                                                       aGTACACCACCGTGGACGATCGCGTCTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGCACCGGGCCGGCGAGCAGGACGTAACGAGGACGACGCGCGAATG‑AAAACAACGCAGATTCTTCGGCATACCg                                                                            >  2:7135928/1‑150 (MQ=255)
                                                                        gTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCgg                                                                           <  1:4577303/150‑1 (MQ=255)
                                                                             ccaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTAc                                                                      <  1:5295488/150‑1 (MQ=255)
                                                                               accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                    <  1:7522273/150‑1 (MQ=255)
                                                                                   tGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCCGCGAGCAGGACTTAAAGAGGACGACGAGCGAAAG‑AAAACAACGAAGATTCTTATGCATACCGGTGTAATGGccc                                                                >  2:6977761/1‑150 (MQ=255)
                                                                                    ggACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTAATGGCCCt                                                               >  1:10638591/1‑150 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  2:3394509/150‑1 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  2:8226038/150‑1 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  1:10129403/150‑1 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  2:2570427/150‑1 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          >  2:11097203/1‑150 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  1:6496833/150‑1 (MQ=255)
                                                                                          tCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCct                                                         >  1:4759003/1‑150 (MQ=255)
                                                                                            gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑CAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                       >  1:2433626/1‑150 (MQ=255)
                                                                                            gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                       <  1:6661842/150‑1 (MQ=255)
                                                                                             cgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                      >  2:469307/1‑150 (MQ=255)
                                                                                                cTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                    >  1:1203463/1‑150 (MQ=255)
                                                                                                 ttCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGcc                                                  >  2:3887281/1‑150 (MQ=255)
                                                                                                       gcgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAg                                            <  2:10540896/150‑1 (MQ=255)
                                                                                                        cgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAgg                                           <  1:5096883/150‑1 (MQ=255)
                                                                                                         gcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGt                                          >  1:7006250/1‑150 (MQ=255)
                                                                                                          cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                         >  1:7748055/1‑150 (MQ=255)
                                                                                                          cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                         >  1:7673115/1‑150 (MQ=255)
                                                                                                            cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                       <  1:7334745/150‑1 (MQ=255)
                                                                                                            cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                       >  2:6681038/1‑150 (MQ=255)
                                                                                                            cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                       >  1:9309608/1‑150 (MQ=255)
                                                                                                             aGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATg                                      <  2:6124047/150‑1 (MQ=255)
                                                                                                             aGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATg                                      <  2:2667035/150‑1 (MQ=255)
                                                                                                               cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                    <  1:1380533/150‑1 (MQ=255)
                                                                                                                  cGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcggc                                 <  2:9349917/150‑1 (MQ=255)
                                                                                                                         aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                          <  2:1001452/150‑1 (MQ=255)
                                                                                                                          aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                         <  1:6272535/150‑1 (MQ=255)
                                                                                                                              cTGATGTACGCCGGACCGGAGCGTCCCGGGCCGGCGAGCAGGACTTTACGAGGACGACGAGCGAATG‑AAAACAACGAATATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCAAGGTGATGGCGGCGGTATCCGCcga                     <  2:10421750/150‑1 (MQ=255)
                                                                                                                                gATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                   <  1:8380119/150‑1 (MQ=255)
                                                                                                                                 aTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacga                  <  1:5731144/150‑1 (MQ=255)
                                                                                                                                   gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAgg                <  2:1793360/150‑1 (MQ=255)
                                                                                                                                       gCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGCAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcggc            <  1:2933988/150‑1 (MQ=255)
                                                                                                                                       cccGGCCCGGTGCGCACCGGGCCGGCGAGCACGACAGAACGAGGACGACGAGCGAAAG‑ACAACAACGAAGATTCTTCGGCATACCGGAGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcgcc            <  1:3125685/149‑3 (MQ=255)
                                                                                                                                         cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGATGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCa          >  1:10800112/1‑150 (MQ=255)
                                                                                                                                          ggCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCaa         >  2:8163667/1‑150 (MQ=255)
                                                                                                                                           gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg        >  2:9554130/1‑150 (MQ=255)
                                                                                                                                             ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt      <  2:8398167/150‑1 (MQ=255)
                                                                                                                                             ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt      <  1:2541646/150‑1 (MQ=255)
                                                                                                                                               ggTGCGAACCGGGCCGGCGAGCAGGACTTGACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg    >  2:5595001/1‑150 (MQ=255)
                                                                                                                                               ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg    >  2:4472707/1‑150 (MQ=255)
                                                                                                                                               ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg    >  1:9278963/1‑150 (MQ=255)
                                                                                                                                               ggTGCGAACCGGGCCGGCGAGCAGGACTAAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg    >  1:5003703/1‑150 (MQ=255)
                                                                                                                                                 tGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGc  >  2:8641794/1‑150 (MQ=255)
                                                                                                                                                  |||                                                                                                                                                   
CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑