BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,198,146	Δ1 bp	coding (230/1980 nt)	PA14_RS14610 ←	cation/acetate symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,198,144	0	G	.	99.2%	1960.8 / 4.4	402	coding (232/1980 nt)	PA14_RS14610	cation/acetate symporter
Reads supporting (aligned to +/- strand): ref base G (1/0); major base . (201/197); minor base C (1/2); total (203/199)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

GGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGC  >  NC_008463/3197995‑3198289

gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCGGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAg                                                                                                                                                   >  2:7685376/1‑150 (MQ=255)

  gCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                 <  1:9428247/150‑2 (MQ=255)

   cGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggca                                                                                                                                                <  1:2355392/150‑3 (MQ=255)

    gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcac                                                                                                                                               >  1:9105770/1‑147 (MQ=255)

    gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcac                                                                                                                                               <  2:8901314/150‑4 (MQ=255)

     aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                              >  1:7909119/1‑146 (MQ=255)

     aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                              >  1:9251230/1‑146 (MQ=255)

       gCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGACGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGAC‑ACGGCACaga                                                                                                                                           <  2:5573514/150‑1 (MQ=255)

         cGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGACTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaag                                                                                                                                         <  2:10243572/150‑1 (MQ=255)

         cGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaag                                                                                                                                         >  2:10550799/1‑150 (MQ=255)

            cagcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAac                                                                                                                                      <  2:8736732/150‑1 (MQ=255)

             agcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCACCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAaca                                                                                                                                     <  1:1299302/150‑1 (MQ=255)

               cagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACg                                                                                                                                   <  1:336803/150‑1 (MQ=255)

                  gCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAAc                                                                                                                                >  1:1474499/1‑150 (MQ=255)

                    cGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAg                                                                                                                              >  2:3877775/1‑150 (MQ=255)

                    cGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAg                                                                                                                              >  2:3639325/1‑150 (MQ=255)

                     ggAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAgg                                                                                                                             >  1:6098198/1‑150 (MQ=255)

                      gAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGa                                                                                                                            <  1:3175167/150‑1 (MQ=255)

                          gCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAacac                                                                                                                        >  2:6226518/1‑150 (MQ=255)

                           cAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACg                                                                                                                       >  2:2692121/1‑150 (MQ=255)

                            aGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                      >  1:6257346/1‑150 (MQ=255)

                            aGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                      <  1:2178641/150‑1 (MQ=255)

                                 cAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                 <  2:2083180/150‑1 (MQ=255)

                                 cAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                 >  1:10617649/1‑150 (MQ=255)

                                  aaCGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggccg                                                                                                                <  1:5058491/150‑1 (MQ=255)

                                   aCGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCcgc                                                                                                               >  1:1420596/1‑150 (MQ=255)

                                    cGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCcgcg                                                                                                              >  2:3074906/1‑150 (MQ=255)

                                    cGCCAGGGGAAGCAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGATGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCcgcg                                                                                                              <  2:845175/150‑1 (MQ=255)

                                     gCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGa                                                                                                             <  2:1136001/150‑1 (MQ=255)

                                        aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAccc                                                                                                          >  1:11071231/1‑150 (MQ=255)

                                             aaGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAg                                                                                                     >  1:8887343/1‑150 (MQ=255)

                                              aGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAgg                                                                                                    >  1:10132102/1‑150 (MQ=255)

                                               ggAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGt                                                                                                   <  1:5238030/150‑1 (MQ=255)

                                               ggAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGGACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGt                                                                                                   >  2:5856049/1‑150 (MQ=255)

                                                   cttcttCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATg                                                                                               <  1:4493800/150‑1 (MQ=255)

                                                     tcttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGtt                                                                                             >  1:10233043/1‑150 (MQ=255)

                                                       ttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGACTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCg                                                                                           <  2:1308735/150‑1 (MQ=255)

                                                          aTGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                        <  1:5610627/150‑1 (MQ=255)

                                                            gcgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGccc                                                                                      >  2:5520686/1‑150 (MQ=255)

                                                             cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCt                                                                                     <  2:6730/150‑1 (MQ=255)

                                                             cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCt                                                                                     <  2:6799/150‑1 (MQ=255)

                                                              gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                    <  2:7329362/150‑1 (MQ=255)

                                                              gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGGCAGGTTCGAGCCCTg                                                                                    >  2:10014479/1‑150 (MQ=255)

                                                              gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCAGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                    >  1:9049183/1‑150 (MQ=255)

                                                               ccccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                   >  1:2513749/1‑150 (MQ=255)

                                                               ccccGCACCTATTCCAGCCCAGCGACCATAGCCATGGTAAGGCGATCGAATTCTCCGTTGGCGCGGTACACATAGATCGACGACAA‑GGCAAAGAAGAACACGAACAGTAACACTCCGGCCGCGACCCCGAATGACAACTTCGATCCCTgc                                                                                   >  2:1784688/1‑150 (MQ=255)

                                                                cccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcg                                                                                  >  1:4856672/1‑150 (MQ=255)

                                                                    ctcctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                              >  1:8017147/1‑150 (MQ=255)

                                                                     tcctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGa                                                                             >  2:9118671/1‑150 (MQ=255)

                                                                      cctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGAc                                                                            >  2:9620746/1‑150 (MQ=255)

                                                                       ctctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACg                                                                           >  2:7162715/1‑150 (MQ=255)

                                                                        tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGc                                                                          <  2:5005933/150‑1 (MQ=255)

                                                                          taccagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCt                                                                        <  1:6871977/148‑1 (MQ=255)

                                                                             cagcccagcGACCAGAGCCTGGGTCAGGCGATCGACTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGACGAACACGAACAGGAACCCGCCGGCCTCGACCCCGACGGTCATGTTCGAGCCCAGCGCCAGACGCCTGGc                                                                     <  2:10372940/150‑1 (MQ=255)

                                                                             cagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGc                                                                     >  1:9811918/1‑150 (MQ=255)

                                                                               gcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCaa                                                                   <  1:7037775/150‑1 (MQ=255)

                                                                                   agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAg                                                               >  1:3869727/1‑150 (MQ=255)

                                                                                    gcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                              >  1:5424747/1‑150 (MQ=255)

                                                                                     cGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCt                                                             <  1:1765302/150‑1 (MQ=255)

                                                                                       aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCg                                                           >  1:19742/1‑150 (MQ=255)

                                                                                        ccAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGACCAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                          <  2:4733904/150‑1 (MQ=255)

                                                                                        ccAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                          >  1:7795515/1‑150 (MQ=255)

                                                                                             gCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAg                                                     <  2:5176858/150‑1 (MQ=255)

                                                                                               cTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGaa                                                   >  1:1866016/1‑150 (MQ=255)

                                                                                                tGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAAc                                                  <  1:736160/150‑1 (MQ=255)

                                                                                                   gTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAt                                               <  1:7425090/150‑1 (MQ=255)

                                                                                                     cAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAc                                             >  1:8220941/1‑150 (MQ=255)

                                                                                                      aGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAcc                                            >  2:7436735/1‑150 (MQ=255)

                                                                                                       ggCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCa                                           <  1:4959489/150‑1 (MQ=255)

                                                                                                       ggCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCa                                           <  2:4316448/150‑1 (MQ=255)

                                                                                                        gCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAg                                          <  1:8586862/150‑1 (MQ=255)

                                                                                                             cGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGTACAGGAACACGCCGGCCGCGACCCCGTAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCCGCTCGGGGCAGAACGATACCAGCGCGa                                     >  2:7828998/1‑150 (MQ=255)

                                                                                                              gAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGAt                                    >  2:3478514/1‑150 (MQ=255)

                                                                                                              gAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGAt                                    >  1:4165830/1‑150 (MQ=255)

                                                                                                               aaTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATg                                   >  2:5123124/1‑150 (MQ=255)

                                                                                                               aaTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATg                                   >  1:286552/1‑150 (MQ=255)

                                                                                                                   cGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGc                               <  1:4295358/150‑1 (MQ=255)

                                                                                                                      cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGt                            >  2:6952132/1‑150 (MQ=255)

                                                                                                                       gTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTg                           >  2:2439468/1‑150 (MQ=255)

                                                                                                                        ttGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTgg                          >  2:8711687/1‑150 (MQ=255)

                                                                                                                          ggCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGac                        >  1:4391558/1‑149 (MQ=255)

                                                                                                                            cgcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACg                      <  1:2978984/150‑1 (MQ=255)

                                                                                                                             gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGt                     >  1:7215690/1‑150 (MQ=255)

                                                                                                                              cgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                    <  2:6492156/150‑1 (MQ=255)

                                                                                                                                gTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGc                  <  2:2975716/150‑1 (MQ=255)

                                                                                                                                   cacaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAg               <  1:3652297/150‑1 (MQ=255)

                                                                                                                                    acaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAgg              >  2:9527298/1‑150 (MQ=255)

                                                                                                                                    acaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAgg              >  1:5543602/1‑150 (MQ=255)

                                                                                                                                     caTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGa             >  2:2577041/1‑150 (MQ=255)

                                                                                                                                      aTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGAt            <  1:8260653/150‑1 (MQ=255)

                                                                                                                                       tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAGACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc           <  2:4507882/150‑1 (MQ=255)

                                                                                                                                        agagCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca          >  1:661160/1‑150 (MQ=255)

                                                                                                                                         gagCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcag         >  1:2984609/1‑150 (MQ=255)

                                                                                                                                             gcCGACAA‑GGCACAGAAGAGCACGAAGAGGAACTCGCCGGCCGCGACCCCGAAGGGCCTGTTCGAGCCCTGCGCCAGACGGCTGGCAATCAGCTGGGGGCGGATCGATTCCGGCGCGATGTTGCCGTGGCACGTGGCCAGGATCagccgg     >  2:10939260/1‑150 (MQ=255)

                                                                                                                                              ccGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga    >  2:1507371/1‑150 (MQ=255)

                                                                                                                                              ccGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga    >  1:6797708/1‑150 (MQ=255)

                                                                                                                                               cGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggag   <  2:8217583/150‑1 (MQ=255)

                                                                                                                                                gACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc  <  2:2253198/150‑1 (MQ=255)

                                                                                                                                                gACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc  <  1:5902274/150‑1 (MQ=255)

                                                                                                                                                gACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc  <  2:8017147/150‑1 (MQ=255)

GGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGC  >  NC_008463/3197995‑3198289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`