Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,978,469 Δ1 bp intergenic (+125/‑20) PA14_RS31455 → / → PA14_RS22760 hypothetical protein/inorganic phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,978,4690C.100.0% 2239.6 / NA 450intergenic (+125/‑20)PA14_RS31455/PA14_RS22760hypothetical protein/inorganic phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (232/218);  total (232/218)

Only 100 of 452 total aligned reads displayed.

CGACAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCGCTCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTGGCCACCGT  >  NC_008463/4978324‑4978613
                                                                                                                                                 |                                                                                                                                                
cGACAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCg‑tctct                                                                                                                                             >  2:3983289/1‑150 (MQ=255)
 gACAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTg                                                                                                                                            <  2:8563851/150‑1 (MQ=255)
  aCAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGa                                                                                                                                           >  1:4908326/1‑150 (MQ=255)
   cAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGAc                                                                                                                                          >  2:1873621/1‑150 (MQ=255)
     ggAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCg                                                                                                                                        >  1:6622338/1‑150 (MQ=255)
      gagaGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCgg                                                                                                                                       >  1:6153998/1‑150 (MQ=255)
        gagCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGtt                                                                                                                                     >  1:7411101/1‑150 (MQ=255)
         agCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGttt                                                                                                                                    >  2:7510017/1‑150 (MQ=255)
            gACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTtctc                                                                                                                                 <  1:5857399/150‑1 (MQ=255)
             aCGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTca                                                                                                                                <  2:10077287/150‑1 (MQ=255)
             aCGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTca                                                                                                                                >  2:9525886/1‑150 (MQ=255)
              cGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTcac                                                                                                                               <  1:9032865/150‑1 (MQ=255)
                 aTAACGAAGCGTTTCACTCTGTTTCGTTTTTCCCGCTAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGAAGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATg                                                                                                                            <  2:1135720/150‑1 (MQ=255)
                 aTAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATg                                                                                                                            >  1:7832194/1‑150 (MQ=255)
                  tAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGt                                                                                                                           <  2:3572355/150‑1 (MQ=255)
                   aaCGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGtt                                                                                                                          >  2:8700120/1‑150 (MQ=255)
                   aaCGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGtt                                                                                                                          <  1:3983768/150‑1 (MQ=255)
                     cGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCg                                                                                                                        >  1:2412779/1‑150 (MQ=255)
                       aaGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGAt                                                                                                                      >  1:3225853/1‑150 (MQ=255)
                       aaGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGAt                                                                                                                      <  2:1072509/150‑1 (MQ=255)
                         gCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCt                                                                                                                    <  1:8777731/150‑1 (MQ=255)
                          cGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCtt                                                                                                                   >  2:2742378/1‑150 (MQ=255)
                           gTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCttt                                                                                                                  >  1:5826184/1‑150 (MQ=255)
                           gTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCttt                                                                                                                  <  1:463588/150‑1 (MQ=255)
                            tttCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAATCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCtttt                                                                                                                 >  2:3829511/1‑150 (MQ=255)
                            tttCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCtttt                                                                                                                 <  2:10922077/150‑1 (MQ=255)
                            tttCAATCAGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCtttt                                                                                                                 <  2:9769305/150‑1 (MQ=255)
                                  tCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCgg                                                                                                           >  2:1585767/1‑150 (MQ=255)
                                  tCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCgg                                                                                                           <  1:3322039/150‑1 (MQ=255)
                                   cTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCggg                                                                                                          <  1:9979224/150‑1 (MQ=255)
                                      tttCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTc                                                                                                       <  1:2027388/150‑1 (MQ=255)
                                       ttCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCg                                                                                                      >  2:6077642/1‑150 (MQ=255)
                                           tttttCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGc                                                                                                  >  1:5966523/1‑150 (MQ=255)
                                           tttttCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTACCGGAGTTCAAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGc                                                                                                  >  1:1252001/1‑150 (MQ=255)
                                            ttttCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGcc                                                                                                 >  1:10048207/1‑150 (MQ=255)
                                            ttttCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGcc                                                                                                 >  2:4208458/1‑150 (MQ=255)
                                             tttCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCt                                                                                                <  1:11095372/150‑1 (MQ=255)
                                                cccGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCtggg                                                                                             <  2:2049731/150‑1 (MQ=255)
                                                cccGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCtggg                                                                                             <  2:259585/150‑1 (MQ=255)
                                                 ccGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAGCCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCtgggt                                                                                            <  1:9732241/150‑1 (MQ=255)
                                                   gCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCtgggtgg                                                                                          >  1:7985355/1‑150 (MQ=255)
                                                        gCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATc                                                                                     <  2:8216707/150‑1 (MQ=255)
                                                         cccGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCa                                                                                    <  2:5741075/150‑1 (MQ=255)
                                                         cccGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCa                                                                                    >  1:3623967/1‑150 (MQ=255)
                                                             aTGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAACAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCt                                                                                <  2:5981728/150‑1 (MQ=255)
                                                             aTGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCt                                                                                <  2:71962/150‑1 (MQ=255)
                                                              tGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTg                                                                               <  1:6867738/150‑1 (MQ=255)
                                                                 gACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTg                                                                            >  1:9239687/1‑150 (MQ=255)
                                                                 gACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTg                                                                            >  2:617096/1‑150 (MQ=255)
                                                                     gggAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGATCGATGCCTGGGTGGGTATCAGCCTGGTGctgg                                                                        <  2:4961057/150‑1 (MQ=255)
                                                                      ggAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggc                                                                       >  2:8042115/1‑150 (MQ=255)
                                                                       gAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggcc                                                                      >  1:6487435/1‑150 (MQ=255)
                                                                        aGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggccc                                                                     <  1:1761516/150‑1 (MQ=255)
                                                                         gTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggccct                                                                    >  1:2831650/1‑150 (MQ=255)
                                                                          ttGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggccctg                                                                   <  2:7970678/150‑1 (MQ=255)
                                                                             cccGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggccctggcc                                                                <  1:10143956/150‑1 (MQ=255)
                                                                             cccGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGctggccctggcc                                                                <  1:5377645/150‑1 (MQ=255)
                                                                               cgcgACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCtt                                                              <  1:9506547/150‑1 (MQ=255)
                                                                               cgcgACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCtt                                                              <  2:2899097/150‑1 (MQ=255)
                                                                               cgcgACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCGGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCtt                                                              <  2:2333932/150‑1 (MQ=255)
                                                                               cgcgACAATGGCAACCGTCCGGAGTTCCAGACGGATAAAACCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCtt                                                              <  2:12379/150‑1 (MQ=255)
                                                                                  gACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGt                                                           >  1:1910141/1‑150 (MQ=255)
                                                                                   aCAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTc                                                          >  1:3371660/1‑150 (MQ=255)
                                                                                        tgCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATACTCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCAc                                                     <  1:10284341/149‑1 (MQ=255)
                                                                                          cAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCt                                                   <  2:1805250/150‑1 (MQ=255)
                                                                                           aaCCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCtt                                                  <  2:3225853/150‑1 (MQ=255)
                                                                                                tCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGt                                             <  1:10773386/150‑1 (MQ=255)
                                                                                                tCCGGAGTTCCAGAAGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTGCGAGt                                             >  2:7076999/1‑150 (MQ=255)
                                                                                                    gAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTtcat                                         <  2:6601731/150‑1 (MQ=255)
                                                                                                     aGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTtcatc                                        >  2:8367795/1‑150 (MQ=255)
                                                                                                     aGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTtcatc                                        <  1:3713975/150‑1 (MQ=255)
                                                                                                        tCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAAc                                     <  2:4405335/150‑1 (MQ=255)
                                                                                                        tCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGGTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAAc                                     >  2:9078120/1‑150 (MQ=255)
                                                                                                         ccAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACg                                    >  2:7782340/1‑150 (MQ=255)
                                                                                                          cAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACgg                                   >  1:1336525/1‑150 (MQ=255)
                                                                                                          cAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACgg                                   <  1:10560553/150‑1 (MQ=255)
                                                                                                          cAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACgg                                   <  1:3929251/150‑1 (MQ=255)
                                                                                                           aGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGc                                  >  1:9321506/1‑150 (MQ=255)
                                                                                                            gACGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCt                                 <  1:2215553/150‑1 (MQ=255)
                                                                                                              cGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTc                               <  2:4720942/150‑1 (MQ=255)
                                                                                                              cGGATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTc                               >  1:3428510/1‑150 (MQ=255)
                                                                                                               ggATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTcc                              >  1:5070474/1‑150 (MQ=255)
                                                                                                               ggATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTcc                              >  1:381042/1‑150 (MQ=255)
                                                                                                                gATAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCa                             <  2:927608/150‑1 (MQ=255)
                                                                                                                 aTAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCAc                            >  1:515142/1‑150 (MQ=255)
                                                                                                                 aTAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCAc                            <  1:981568/150‑1 (MQ=255)
                                                                                                                  tAAAAACGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACg                           >  1:7269206/1‑150 (MQ=255)
                                                                                                                   aaaaaCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGa                          >  2:6339020/1‑150 (MQ=255)
                                                                                                                   aaaaaCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGa                          <  2:5070881/150‑1 (MQ=255)
                                                                                                                     aaaCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGaca                        >  1:4540005/1‑150 (MQ=255)
                                                                                                                      aaCGCATCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGacac                       >  2:8900651/1‑150 (MQ=255)
                                                                                                                            tCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCaa                 <  2:692076/150‑1 (MQ=255)
                                                                                                                            tCTGGCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCaa                 >  1:8305274/1‑150 (MQ=255)
                                                                                                                               ggCATAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAAcgc              >  1:4332058/1‑150 (MQ=255)
                                                                                                                                  aTAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGt           <  1:8543400/150‑1 (MQ=255)
                                                                                                                                   tAATCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTg          >  1:415689/1‑150 (MQ=255)
                                                                                                                                    aaTCGCCGCCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTgg         >  2:10262974/1‑150 (MQ=255)
                                                                                                                                       cgccgcCCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCAGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTGGCCa      >  2:2865327/1‑150 (MQ=255)
                                                                                                                                        gccgccCCG‑TCTCTGACCGGTTTCTCACATGATCTATCTTATAATCTGGCACTATGCCAGGTACCGTATCAGCATGGTGCACGAACTGTCCTTCATCATCAACATAGAGATCATCATCGGCTTCCACGACACTGACAACTCGTTGGcaaa     >  2:7342523/1‑147 (MQ=255)
                                                                                                                                         ccgccCCG‑TCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTGGCCAcc    >  1:7151584/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGACAGGAGAGCGACGGATAACGAAGCGTTTCAATCTGTTTCGTTTTTCCCGCAAAGCCCGATGCGACGGGGAGTTGCCCGCGACAATGGCAACCTTCCGGAGTTCCAGACGGATAAAAACGCATCTGGCATAATCGCCGCCCCGCTCTCTGACCGGTTTCTCACATGTTCGATCTTTTCAGCGGGCTCGATGCCTGGGTGGGTATCAGCCTGGTGCTGGCCCTGGCCTTCGTCCTCACCTTCGAGTTCATCAACGGCTTCCACGACACTGCCAACGCGGTGGCCACCGT  >  NC_008463/4978324‑4978613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑