BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,358,513	Δ1 bp	pseudogene (774/1285 nt)	PA14_RS24540 →	site‑specific integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,358,510	0	T	.	99.7%	661.6 / ‑3.5	301	pseudogene (771/1285 nt)	PA14_RS24540	site‑specific integrase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (132/168); total (132/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

GATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAT  >  NC_008463/5358361‑5358655

gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                   >  1:7379651/1‑150 (MQ=32)

gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                   >  2:5291182/1‑150 (MQ=32)

 aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                  >  1:10353381/1‑150 (MQ=32)

 aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                  <  2:349328/150‑1 (MQ=32)

  tAGAGCCGGATTCGCCGATGACGCCCAGTTGTTCAACGACAACCGGTTCTCACCGCACTACACGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGGGCGGATGCCCCCGCACCGttt                                                                                                                                                 <  2:548701/150‑1 (MQ=11)

  tAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                 >  2:8909152/1‑150 (MQ=32)

   agagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttc                                                                                                                                                <  1:5159086/150‑2 (MQ=14)

    gagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttcc                                                                                                                                               <  2:1906786/150‑3 (MQ=11)

     agCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                              >  2:10418429/1‑147 (MQ=1)

     agCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                              >  2:7453829/1‑147 (MQ=1)

                                                    cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                              >  1:3045538/1‑150 (MQ=1)

                                                    cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                              <  2:1433692/150‑1 (MQ=1)

                                                      ccGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                            >  2:3118501/1‑150 (MQ=11)

                                                      ccGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                            >  1:5782485/1‑150 (MQ=11)

                                                      ccGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                            >  1:7734225/1‑150 (MQ=11)

                                                       cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAcc                                                                                           >  2:2258180/1‑150 (MQ=11)

                                                       cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAcc                                                                                           >  2:7197423/1‑150 (MQ=11)

                                                        gCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                          <  1:10614878/150‑1 (MQ=11)

                                                         cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                         >  1:4129681/1‑150 (MQ=11)

                                                         cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                         <  1:2594123/150‑1 (MQ=11)

                                                          aCTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                        <  1:10027430/150‑1 (MQ=11)

                                                           cTACAAGAGCAAGGTGATGATCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCAc                                                                                       <  2:9049460/150‑1 (MQ=2)

                                                            tACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCCCACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                      >  2:8947935/1‑150 (MQ=2)

                                                              cAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCTAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGaa                                                                                    >  1:8083127/1‑150 (MQ=2)

                                                              cAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGaa                                                                                    >  2:8610234/1‑150 (MQ=11)

                                                               aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAg                                                                                   <  2:1080367/150‑1 (MQ=11)

                                                                agagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                  <  1:8429447/150‑1 (MQ=11)

                                                                agagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                  <  1:6358781/150‑1 (MQ=11)

                                                                 gagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTg                                                                                 >  1:3258269/1‑150 (MQ=11)

                                                                   gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGcc                                                                               >  1:4724773/1‑150 (MQ=11)

                                                                   gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGcc                                                                               <  2:6711959/150‑1 (MQ=11)

                                                                       ggTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGTGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCGGGCCACCGACTGGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCt                                                                           <  2:416962/150‑1 (MQ=255)

                                                                         tgatgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                         >  1:5543524/1‑150 (MQ=11)

                                                                           atgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGACCATTCACCTCACGAAGTGCCTGCTCAAc                                                                       >  2:10209439/1‑150 (MQ=2)

                                                                            tgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                      >  2:2727543/1‑150 (MQ=11)

                                                                            tgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                      <  2:921238/150‑1 (MQ=11)

                                                                             gaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCa                                                                     <  2:8374459/150‑1 (MQ=11)

                                                                             gaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCa                                                                     >  1:4369100/1‑150 (MQ=11)

                                                                              aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCAc                                                                    <  2:4870442/150‑1 (MQ=11)

                                                                              aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCAc                                                                    >  2:6552608/1‑150 (MQ=11)

                                                                              aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCAc                                                                    <  1:3943488/150‑1 (MQ=11)

                                                                                  ccGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGa                                                                >  1:8576166/1‑150 (MQ=11)

                                                                                    gACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAat                                                              >  2:591620/1‑150 (MQ=11)

                                                                                     aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                             >  2:1478454/1‑150 (MQ=11)

                                                                                     aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                             >  1:6621854/1‑150 (MQ=11)

                                                                                     aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                             >  2:3137829/1‑150 (MQ=11)

                                                                                         ggTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                         >  1:4584173/1‑150 (MQ=11)

                                                                                         ggTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                         <  1:5312419/150‑1 (MQ=11)

                                                                                          gTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAg                                                        <  1:6912676/150‑1 (MQ=11)

                                                                                           tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGa                                                       <  2:9623039/150‑1 (MQ=11)

                                                                                           tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGa                                                       <  1:3572973/150‑1 (MQ=11)

                                                                                              aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                    <  2:3862379/150‑1 (MQ=11)

                                                                                              aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                    <  1:6716073/150‑1 (MQ=11)

                                                                                                gCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACg                                                  <  1:1487596/150‑1 (MQ=14)

                                                                                                  cATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGAt                                                <  1:2788438/150‑1 (MQ=17)

                                                                                                   aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATg                                               <  1:5239699/150‑1 (MQ=17)

                                                                                                   aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATg                                               <  1:5908412/150‑1 (MQ=17)

                                                                                                   aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATg                                               <  2:3619041/150‑1 (MQ=17)

                                                                                                     gTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAg                                             >  1:6031820/1‑150 (MQ=17)

                                                                                                      tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGc                                            <  1:7032743/150‑1 (MQ=17)

                                                                                                      tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGc                                            >  1:6192427/1‑150 (MQ=17)

                                                                                                       aCCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                           <  2:1734700/150‑1 (MQ=17)

                                                                                                         cGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTAc                                         <  2:4456520/150‑1 (MQ=17)

                                                                                                              gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                    <  1:6240517/150‑1 (MQ=17)

                                                                                                               ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                   <  2:8726056/150‑1 (MQ=17)

                                                                                                               ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                   >  2:3951475/1‑150 (MQ=17)

                                                                                                               ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                   <  1:3893669/150‑1 (MQ=17)

                                                                                                                tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAgg                                  <  2:5639490/150‑1 (MQ=17)

                                                                                                                 gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                 >  1:7475443/1‑150 (MQ=17)

                                                                                                                 gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                 >  1:10955039/1‑150 (MQ=17)

                                                                                                                 gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                 >  2:4999407/1‑150 (MQ=17)

                                                                                                                 gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                 <  1:9645328/150‑1 (MQ=17)

                                                                                                                  aCCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                >  2:3961833/1‑150 (MQ=17)

                                                                                                                   ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCg                               >  2:6986337/1‑150 (MQ=17)

                                                                                                                     gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGAc                             <  2:4107487/150‑1 (MQ=17)

                                                                                                                        aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCTACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                          >  2:11096130/1‑150 (MQ=12)

                                                                                                                        aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                          <  1:8837527/150‑1 (MQ=17)

                                                                                                                        aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                          >  1:1304830/1‑150 (MQ=17)

                                                                                                                        aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCACCCCACCGACTTGATGCAGGCACCCGAGCGGAACATTCACCTCACCACGTGCCCGCACAACCACTCGAATCTCCAGACCCCGATGAGCTACATCGAGGCCGACTAc                          <  2:5091538/150‑1 (MQ=11)

                                                                                                                          ggTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGa                        <  1:2245750/150‑1 (MQ=17)

                                                                                                                           gTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                       <  1:1886780/150‑1 (MQ=18)

                                                                                                                           gTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                       >  1:595966/1‑150 (MQ=18)

                                                                                                                           gTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                       >  1:7182001/1‑150 (MQ=18)

                                                                                                                            ttGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATc                      >  1:1737049/1‑150 (MQ=21)

                                                                                                                              ggggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcac                    <  2:2498061/150‑1 (MQ=25)

                                                                                                                               gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcaca                   <  2:3972725/150‑1 (MQ=25)

                                                                                                                                 gTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATg                 <  1:9529660/150‑1 (MQ=25)

                                                                                                                                  tGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGc                <  2:6229815/150‑1 (MQ=25)

                                                                                                                                   gCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcg               <  1:4878680/150‑1 (MQ=25)

                                                                                                                                    cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgt              <  1:5053409/150‑1 (MQ=25)

                                                                                                                                    cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgt              <  1:9322117/150‑1 (MQ=25)

                                                                                                                                       aTGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgcc           >  2:10338850/1‑150 (MQ=25)

                                                                                                                                         gACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgt         >  2:807390/1‑150 (MQ=25)

                                                                                                                                           ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGTGCTACATCGAGGCCGACTACGATCACATGcgtgccgtgc       <  1:1077626/150‑1 (MQ=19)

                                                                                                                                             ccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgctg     >  2:9386968/1‑150 (MQ=17)

                                                                                                                                              cGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgctgc    >  2:3060680/1‑150 (MQ=17)

                                                                                                                                               gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa   >  1:6852195/1‑150 (MQ=17)

                                                                                                                                               gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa   >  1:2373545/1‑150 (MQ=17)

                                                                                                                                               gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa   <  2:3269464/150‑1 (MQ=17)

                                                                                                                                                cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAt  <  1:6505269/150‑1 (MQ=17)

GATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAT  >  NC_008463/5358361‑5358655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`