BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	6,070,093	Δ1 bp	pseudogene (562/1086 nt)	PA14_RS27735 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	6,070,092	0	A	.	99.8%	2886.2 / ‑3.7	582	pseudogene (561/1086 nt)	PA14_RS27735	oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (257/324); minor base C (0/1); total (257/325)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 606 total aligned reads displayed.

CCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAG  >  NC_008463/6069942‑6070238

ccTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCa                                                                                                                                                    >  2:1442780/1‑150 (MQ=255)

 cTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCac                                                                                                                                                   >  1:7990615/1‑149 (MQ=255)

      cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgct                                                                                                                                              <  2:8326885/150‑7 (MQ=255)

      cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgct                                                                                                                                              <  1:1375424/150‑7 (MQ=255)

       gTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                                             >  1:7532956/1‑143 (MQ=255)

           aGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGAcc                                                                                                                                        <  2:6802503/150‑1 (MQ=255)

             gccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                                                      <  1:9842647/150‑2 (MQ=255)

              ccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACTCGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCagg                                                                                                                                     >  1:7411188/1‑148 (MQ=255)

              ccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCagg                                                                                                                                     >  2:7214638/1‑148 (MQ=255)

               cgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCaggc                                                                                                                                    >  1:5060171/1‑147 (MQ=255)

               cgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCGGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGAcctggc                                                                                                                                    <  2:2694056/150‑5 (MQ=255)

                    tGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtgt                                                                                                                              >  1:5561709/1‑150 (MQ=255)

                      cAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCGGCGACCGCTGGGGCGTCCACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGGCCGCGGCCCGC‑ACCTGCTCGAGC‑AGGCACTGGGc                                                                                                                            >  2:4223525/1‑150 (MQ=255)

                      cAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGc                                                                                                                            >  1:2412113/1‑150 (MQ=255)

                      cAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGc                                                                                                                            >  1:3537230/1‑150 (MQ=255)

                            ggCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttc                                                                                                                      >  2:1339426/1‑149 (MQ=255)

                             gcagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                                                     <  2:9726201/150‑3 (MQ=255)

                              cagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCgg                                                                                                                    <  1:728873/150‑1 (MQ=255)

                               agcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGc                                                                                                                   <  1:3767204/150‑1 (MQ=255)

                               agcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGc                                                                                                                   >  2:2362394/1‑150 (MQ=255)

                                 caTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                                                 <  2:6662863/150‑1 (MQ=255)

                                 caTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                                                 <  1:10607312/150‑1 (MQ=255)

                                  aTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATa                                                                                                                <  1:915682/150‑1 (MQ=255)

                                   tCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAc                                                                                                               <  1:3032031/150‑1 (MQ=255)

                                   tCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAc                                                                                                               <  1:6106292/150‑1 (MQ=255)

                                      gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                                            >  1:100338/1‑150 (MQ=255)

                                         cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                         >  1:2215636/1‑150 (MQ=255)

                                         cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                         <  2:5207115/150‑1 (MQ=255)

                                         cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                         >  2:1970758/1‑150 (MQ=255)

                                         cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                         <  2:9624744/150‑1 (MQ=255)

                                          ggTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCg                                                                                                        >  1:11030805/1‑150 (MQ=255)

                                          ggTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCg                                                                                                        <  1:7172412/150‑1 (MQ=255)

                                             gcgcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTAt                                                                                                     <  1:658496/150‑1 (MQ=255)

                                                cTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGTGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCa                                                                                                  >  1:9518667/1‑150 (MQ=255)

                                                cTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCa                                                                                                  <  1:5217836/150‑1 (MQ=255)

                                                 tACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGTCCTCGGCCCGC‑ACCTGCTCGACC‑CGGCCCTGTGCCTGTTCGGCATACCGCCGCGTATGCAc                                                                                                 >  2:4596107/1‑150 (MQ=255)

                                                  accgcGAGTCGGCCATCGAGCGCGTCCGGCCGCAGGGCCGCGACCGCTGGCGCGAACACGGCCTGCCCGGC‑TCCGGGCTGTGGGTCGACCGCGGCCCGC‑ACCTGCTCGACC‑GGGCCCGGTGCGTGTTCGGCGTACCGCCGCGGATGCACg                                                                                                >  2:10839537/5‑150 (MQ=255)

                                                    ttCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGa                                                                                              >  2:3012389/1‑150 (MQ=255)

                                                     tCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGac                                                                                             <  1:5485431/150‑1 (MQ=255)

                                                       gAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGacac                                                                                           >  1:3346642/1‑150 (MQ=255)

                                                         gTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCt                                                                                         >  2:9278267/1‑150 (MQ=255)

                                                           cGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgc                                                                                       <  2:3340399/150‑1 (MQ=255)

                                                             gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                     <  1:4879190/150‑1 (MQ=255)

                                                             gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                     >  1:2927025/1‑150 (MQ=255)

                                                             gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                     <  2:6009873/150‑1 (MQ=255)

                                                               cATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                                                   <  1:9025427/150‑1 (MQ=255)

                                                               cATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                                                   >  1:5678463/1‑150 (MQ=255)

                                                               cATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                                                   >  1:9637108/1‑150 (MQ=255)

                                                                 tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                                                 <  2:2452966/150‑1 (MQ=255)

                                                                 tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                                                 <  2:1181876/150‑1 (MQ=255)

                                                                  cGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCt                                                                                >  1:5953564/1‑150 (MQ=255)

                                                                    aGCGATTCAGTACGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTTTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGACTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgc                                                                              >  2:3878896/1‑150 (MQ=255)

                                                                           ccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                                                       <  1:1246179/150‑1 (MQ=255)

                                                                           ccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                                                       >  1:6421539/1‑150 (MQ=255)

                                                                             gtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcg                                                                     <  1:4963843/150‑1 (MQ=255)

                                                                              tccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgc                                                                    >  1:8725486/1‑150 (MQ=255)

                                                                              tccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgc                                                                    <  2:7876023/150‑1 (MQ=255)

                                                                              tccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgc                                                                    >  1:4607363/1‑150 (MQ=255)

                                                                                cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                                                  >  1:5801952/1‑150 (MQ=255)

                                                                                  cAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTg                                                                >  1:10449421/1‑150 (MQ=255)

                                                                                     gTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAcc                                                             >  2:10497688/1‑150 (MQ=255)

                                                                                     gTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAcc                                                             <  1:8976114/150‑1 (MQ=255)

                                                                                      tCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcg                                                            >  2:286006/1‑150 (MQ=255)

                                                                                       ccGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcga                                                           <  2:8577679/150‑1 (MQ=255)

                                                                                           gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCTGGCTGTGGTTCGACCACGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGGTCGGCATACCGCAGCGTCTGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                                                       >  2:4728235/1‑150 (MQ=255)

                                                                                           gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                                                       <  2:8829390/150‑1 (MQ=255)

                                                                                             ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGCGGGCGCGCTGACCGACGACTg                                                     >  2:6295696/1‑150 (MQ=255)

                                                                                             ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTg                                                     >  2:2247339/1‑150 (MQ=255)

                                                                                                cTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGtt                                                  <  2:9474337/150‑1 (MQ=255)

                                                                                                 tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                 <  1:8185614/150‑1 (MQ=255)

                                                                                                    cgcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAg                                              <  1:10311712/150‑1 (MQ=255)

                                                                                                    cgcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAg                                              >  1:4469326/1‑150 (MQ=255)

                                                                                                         acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                         <  1:1624220/150‑1 (MQ=255)

                                                                                                          cacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTg                                        <  1:7702898/150‑1 (MQ=255)

                                                                                                          cacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGGTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTg                                        >  1:8498243/1‑150 (MQ=255)

                                                                                                           acGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGc                                       <  2:2025953/150‑1 (MQ=255)

                                                                                                             gACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTc                                     <  2:8974856/150‑1 (MQ=255)

                                                                                                             gACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTc                                     <  2:8219846/150‑1 (MQ=255)

                                                                                                             gACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCGGCTCGACC‑CGGCACTGTGCCCGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGCCCGACGCCTGGTTCGAGGTCCTGCTc                                     <  2:7511250/150‑1 (MQ=255)

                                                                                                                cTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGAc                                  >  1:6759276/1‑150 (MQ=255)

                                                                                                                  gCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTa                                <  2:5517154/150‑1 (MQ=255)

                                                                                                                  gCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTa                                <  1:10693076/150‑1 (MQ=255)

                                                                                                                   cccGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTAt                               >  1:4239098/1‑150 (MQ=255)

                                                                                                                   cccGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTAt                               <  2:4988218/150‑1 (MQ=255)

                                                                                                                          tCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCAc                         <  1:4014742/150‑1 (MQ=255)

                                                                                                                          tCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCAc                         <  2:1790061/150‑1 (MQ=255)

                                                                                                                               gCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTCTCCGCACTGCCa                    >  2:10241306/1‑150 (MQ=255)

                                                                                                                                cTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAg                   <  2:7646582/150‑1 (MQ=255)

                                                                                                                                    ggTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTgg               <  1:7862710/150‑1 (MQ=255)

                                                                                                                                     gTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgc              <  1:6904049/150‑1 (MQ=255)

                                                                                                                                       tcgACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgcgc            <  2:7991258/148‑1 (MQ=255)

                                                                                                                                        cgACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCt           >  2:4429382/2‑150 (MQ=255)

                                                                                                                                         gACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTg          >  1:4158248/1‑150 (MQ=255)

                                                                                                                                          aCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGCCTGGTTCGAGGTCCTGCTCGACTCTCCGCACTGCCAGGTGGCGCTgc         >  2:5767239/1‑150 (MQ=255)

                                                                                                                                          aCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgc         >  1:9372305/1‑150 (MQ=255)

                                                                                                                                           ccTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcg        <  1:1988450/150‑1 (MQ=255)

                                                                                                                                           ccTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcg        >  1:267942/1‑150 (MQ=255)

                                                                                                                                           ccTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCAGACCGACGACTGGTTCGAGGTCCAGCTCGACTATCCGCACTGCCAGGTGGCGCTgcg        >  1:8056439/1‑150 (MQ=255)

                                                                                                                                              cggCCCGC‑ACCTGCTCGAAC‑AGGCACTGTGCCTGTTCGGCATACCGCTGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcgc     <  2:3480918/149‑1 (MQ=255)

                                                                                                                                                gCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCa   >  2:6988165/1‑150 (MQ=255)

CCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAG  >  NC_008463/6069942‑6070238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`