BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,239,163	(C)_5→4	intergenic (+161/‑32)	PA14_RS05795 → / → lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,239,159	0	C	.	100.0%	2334.6 / NA	469	intergenic (+157/‑36)	PA14_RS05795/lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (261/208); total (261/208)

Only 100 of 490 total aligned reads displayed.

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

aGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCTCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGTGCCGACGGGc                                                                                                                                                   <  2:10245755/150‑1 (MQ=255)

aGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGc                                                                                                                                                   >  1:4734653/1‑150 (MQ=255)

 gcgcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcc                                                                                                                                                  >  2:8491254/1‑150 (MQ=255)

     tGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGccccgt                                                                                                                                              <  1:220660/150‑3 (MQ=255)

         cGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCaga                                                                                                                                         >  1:3439739/1‑150 (MQ=255)

          gAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCagag                                                                                                                                        >  2:4102522/1‑150 (MQ=255)

           aGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGc                                                                                                                                       >  1:7257177/1‑150 (MQ=255)

             cGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCt                                                                                                                                     <  1:9157102/150‑1 (MQ=255)

             cGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCt                                                                                                                                     <  1:6695281/150‑1 (MQ=255)

                   ccTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGATAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                               <  2:7541447/150‑1 (MQ=255)

                     tCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAccc                                                                                                                             >  1:4064121/1‑150 (MQ=255)

                      cGACGGTCTATCGGGCTCTTGGGTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCa                                                                                                                            <  1:10932257/150‑1 (MQ=255)

                         cGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGa                                                                                                                         <  1:9588488/150‑1 (MQ=255)

                           gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                       >  2:5284780/1‑150 (MQ=255)

                           gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                       <  1:5584023/150‑1 (MQ=255)

                             cTATCGGGCTCGTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTACGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgtgt                                                                                                                     >  2:7201951/1‑150 (MQ=255)

                              tATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTc                                                                                                                    <  2:9743471/150‑1 (MQ=255)

                               aTCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                   >  2:2591805/1‑150 (MQ=255)

                               aTCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                   >  1:8467122/1‑150 (MQ=255)

                                 cGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCgg                                                                                                                 <  1:2772311/150‑1 (MQ=255)

                                  gggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGa                                                                                                                <  2:10904485/150‑1 (MQ=255)

                                   ggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGGCGGG‑CCCCGTCAGAGCCTGGCTACCCAGGCGTGTCCGGag                                                                                                               >  2:7405712/1‑149 (MQ=255)

                                      tctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGa                                                                                                            >  1:1963818/1‑150 (MQ=255)

                                      tctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATAGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTAAGAGCCTGGCAACCCAGGAGTGTCCGGATTGa                                                                                                            >  2:1083907/1‑150 (MQ=255)

                                        ttGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATc                                                                                                          <  2:3541180/150‑1 (MQ=255)

                                               ggCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTcc                                                                                                   <  2:2340256/150‑1 (MQ=255)

                                                 ccTGCGCCGTGACAGGCCCGCCGAATCGCTGGATAATGCCGGCTCGTTTCTCCGCGCCATGCCGCCCAGGCCGGAAACGCGTGTTGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTGCGt                                                                                                 >  2:8091989/1‑150 (MQ=255)

                                                 ccTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGt                                                                                                 >  1:2509087/1‑150 (MQ=255)

                                                    gcgcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAt                                                                                              <  1:1839433/150‑1 (MQ=255)

                                                     cgcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAtt                                                                                             <  2:20645/150‑1 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  2:4651955/150‑1 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  1:9727044/150‑1 (MQ=255)

                                                       cAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTg                                                                                           >  2:10034169/1‑150 (MQ=255)

                                                           gACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTccc                                                                                       <  1:5262811/150‑1 (MQ=255)

                                                           gACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCAGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTccc                                                                                       >  2:553683/1‑150 (MQ=255)

                                                            aCAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCg                                                                                      >  1:6586538/1‑150 (MQ=255)

                                                            aCAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCg                                                                                      <  1:5397770/150‑1 (MQ=255)

                                                                  ccgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                <  1:6840883/150‑1 (MQ=255)

                                                                   cgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTg                                                                               >  1:1221775/1‑150 (MQ=255)

                                                                   cgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTg                                                                               >  1:5173066/1‑150 (MQ=255)

                                                                    gccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAGGTGc                                                                              <  2:10483282/150‑1 (MQ=255)

                                                                        aaTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGt                                                                          >  1:4332014/1‑150 (MQ=255)

                                                                        aaTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGt                                                                          <  2:5241126/150‑1 (MQ=255)

                                                                         aTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTc                                                                         >  2:9630056/1‑150 (MQ=255)

                                                                         aTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTc                                                                         >  2:10341588/1‑150 (MQ=255)

                                                                            gctggATAATGCCGGGTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGTCAACCCAGGAGTGTCCGGATGGCTCGTGTTCCGTTATTTGTCCCGGGAAGTGCTGGTCAcc                                                                      >  2:7916904/4‑150 (MQ=255)

                                                                            gCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAcc                                                                      <  1:3157106/150‑1 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     <  2:2943337/150‑1 (MQ=255)

                                                                              aGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGACGTGCTGGTCACCAt                                                                    >  2:8766211/1‑150 (MQ=255)

                                                                              aGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCAt                                                                    <  2:9410768/150‑1 (MQ=255)

                                                                              aGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCAt                                                                    <  2:3952211/150‑1 (MQ=255)

                                                                               ggATAATGCCGGCTATTTTCTCCGCGCCCTGCCGCCATGGCCGGAACCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                   <  2:3463039/150‑1 (MQ=255)

                                                                               ggATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                   <  2:5876906/150‑1 (MQ=255)

                                                                                gATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGa                                                                  <  2:8938282/150‑1 (MQ=255)

                                                                                  tAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgc                                                                >  1:2582131/1‑150 (MQ=255)

                                                                                   aaTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcg                                                               >  2:5088045/1‑150 (MQ=255)

                                                                                   aaTGCCGGCTATGTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcg                                                               <  1:10004029/150‑1 (MQ=255)

                                                                                    aTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcgc                                                              >  1:857536/1‑150 (MQ=255)

                                                                                       ccGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGATACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGt                                                           <  1:10113202/150‑1 (MQ=255)

                                                                                           cTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgc                                                       <  2:2732987/150‑1 (MQ=255)

                                                                                            tATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcg                                                      >  1:10247282/1‑150 (MQ=255)

                                                                                            tATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcg                                                      >  1:8888697/1‑150 (MQ=255)

                                                                                             atattCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCCGAGCCTGGCCACCCAGGAGTGTCCGGATTGATCGTGTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcgc                                                     >  2:4472540/4‑150 (MQ=255)

                                                                                              ttttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGcc                                                    >  2:4767581/1‑150 (MQ=255)

                                                                                               tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGGGATTTGTCCCGTGACGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                   >  2:8671166/1‑150 (MQ=255)

                                                                                                ttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGt                                                  <  2:4163295/150‑1 (MQ=255)

                                                                                                  ctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgc                                                >  2:3049438/1‑150 (MQ=255)

                                                                                                    ccGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgctg                                              >  1:2872152/1‑150 (MQ=255)

                                                                                                    ccGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgctg                                              >  1:393864/1‑150 (MQ=255)

                                                                                                       cgcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGctgctg                                           <  1:4554536/150‑1 (MQ=255)

                                                                                                       cgcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGctgctg                                           >  2:6615337/1‑150 (MQ=255)

                                                                                                        gcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTgg                                          >  2:4396480/1‑150 (MQ=255)

                                                                                                         ccATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGGTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGt                                         >  2:1807468/1‑150 (MQ=255)

                                                                                                          cATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTg                                        >  1:10422001/1‑150 (MQ=255)

                                                                                                           aTGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGa                                       <  1:7474569/150‑1 (MQ=255)

                                                                                                            tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                      >  2:1205752/1‑150 (MQ=255)

                                                                                                             gccgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatc                                     <  1:9503079/150‑1 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 >  2:4873081/1‑150 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAg                             <  1:1355685/150‑1 (MQ=255)

                                                                                                                      gCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGTTCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                            >  2:6952104/1‑150 (MQ=255)

                                                                                                                      gCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                            <  2:5793702/150‑1 (MQ=255)

                                                                                                                             aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCACCCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGtt                     <  2:7810910/150‑1 (MQ=255)

                                                                                                                             aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGtt                     >  2:8345730/1‑150 (MQ=255)

                                                                                                                               gcgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtc                   <  1:7663736/150‑1 (MQ=255)

                                                                                                                                cgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtca                  >  1:8041320/1‑150 (MQ=255)

                                                                                                                                   gatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGGGCTGCTGGTGATCATCATGAGTGGCCGTTtcatca               >  2:4905481/1‑150 (MQ=255)

                                                                                                                                     tgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAg             >  2:9150569/1‑150 (MQ=255)

                                                                                                                                     tgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAg             >  1:7934898/1‑150 (MQ=255)

                                                                                                                                     tgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAg             >  1:10584785/1‑150 (MQ=255)

                                                                                                                                      gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGt            <  2:10158690/150‑1 (MQ=255)

                                                                                                                                      gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGt            >  2:3280644/1‑150 (MQ=255)

                                                                                                                                       aCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTa           <  2:1306895/150‑1 (MQ=255)

                                                                                                                                       aCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTa           >  1:5824360/1‑150 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc         >  2:6223151/1‑150 (MQ=255)

                                                                                                                                          gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt        <  1:3693052/150‑1 (MQ=255)

                                                                                                                                          gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt        <  1:5118323/150‑1 (MQ=255)

                                                                                                                                           ggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTg       <  1:8206639/150‑1 (MQ=255)

                                                                                                                                            gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTgg      <  2:1433059/150‑1 (MQ=255)

                                                                                                                                             ccGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgc     >  1:8610939/1‑150 (MQ=255)

                                                                                                                                                aCGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCa  >  1:6259580/1‑150 (MQ=255)

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`