BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	559,051	+T	intergenic (+619/+70)	PA14_RS02545 → / ← rarD	glycine cleavage system protein R/EamA family transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	559,050	1	.	T	97.3%	1203.2 / 20.4	302	intergenic (+618/+71)	PA14_RS02545/rarD	glycine cleavage system protein R/EamA family transporter RarD
Reads supporting (aligned to +/- strand): ref base . (6/2); new base T (160/131); total (169/133)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

CAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATG  >  NC_008463/558901‑559193

 aGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑t                                                                                                                                                 <  1:8849689/150‑1 (MQ=255)

 aGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑t                                                                                                                                                 <  2:178209/150‑1 (MQ=255)

 aGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑t                                                                                                                                                 <  2:6376716/150‑1 (MQ=255)

   cGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑ttg                                                                                                                                               <  1:411930/150‑3 (MQ=255)

          cTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCggggg                                                                                                                                        <  1:3477239/150‑1 (MQ=255)

           tCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGa                                                                                                                                       <  2:2086747/150‑1 (MQ=255)

            cAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGAt                                                                                                                                      <  2:581379/150‑1 (MQ=255)

             aaaGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATa                                                                                                                                     >  1:8539040/1‑150 (MQ=255)

              aaGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATag                                                                                                                                    >  1:3233184/1‑150 (MQ=255)

               aGACTGATCAGGCGCTTTTCAGGGCTTCTTCCGACGAGTTAGCCACGCGCCTGCGGGGATGAGAAGGCAGTTATATGCACAAATTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATagg                                                                                                                                   <  2:10767943/150‑1 (MQ=255)

                gACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATagga                                                                                                                                  <  1:744158/150‑1 (MQ=255)

                 aCTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaa                                                                                                                                 <  1:48816/150‑1 (MQ=255)

                  cTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaag                                                                                                                                >  2:2853149/1‑150 (MQ=255)

                   tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGAGaggaagg                                                                                                                               >  2:9109906/1‑150 (MQ=255)

                      tCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGt                                                                                                                            <  1:9063071/150‑1 (MQ=255)

                         ggCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCg                                                                                                                         <  2:4935763/150‑1 (MQ=255)

                          gcgcTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCgt                                                                                                                        <  1:2255778/150‑1 (MQ=255)

                           cgcTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCgtg                                                                                                                       >  1:781081/1‑150 (MQ=255)

                             cTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGtt                                                                                                                     >  1:556399/1‑150 (MQ=255)

                              tttGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttt                                                                                                                    <  1:3250915/150‑1 (MQ=255)

                               ttGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGtttt                                                                                                                   <  1:2396360/150‑1 (MQ=255)

                                tGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttttt                                                                                                                  <  2:5661774/150‑1 (MQ=255)

                                   aGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCg                                                                                                               >  2:7093685/1‑150 (MQ=255)

                                      gCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCa                                                                                                            <  1:3848221/150‑1 (MQ=255)

                                      gCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCa                                                                                                            <  1:1406787/150‑1 (MQ=255)

                                        ttcttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGG‑A‑TGGGCGGGGGCTCGGAAGGAGGGCGTGTTTTTTCGCCAgg                                                                                                          >  2:9723887/1‑150 (MQ=255)

                                         tcttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGc                                                                                                         >  2:5545083/1‑150 (MQ=255)

                                         tcttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGc                                                                                                         <  1:8244508/150‑1 (MQ=255)

                                           ttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCtt                                                                                                       <  2:5398355/150‑1 (MQ=255)

                                           ttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCtt                                                                                                       <  1:5295377/150‑1 (MQ=255)

                                           ttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCtt                                                                                                       <  1:2047098/150‑1 (MQ=255)

                                            tcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTg                                                                                                      <  2:9297459/150‑1 (MQ=255)

                                            tcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTg                                                                                                      >  1:7266568/1‑150 (MQ=255)

                                              cgacgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCg                                                                                                    <  1:10082411/150‑1 (MQ=255)

                                                acgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                  >  2:2540109/1‑150 (MQ=255)

                                                acgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑AGT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                  >  2:4488982/1‑150 (MQ=255)

                                                 cgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAg                                                                                                 <  2:8595882/150‑1 (MQ=255)

                                                 cgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAg                                                                                                 >  1:863686/1‑150 (MQ=255)

                                                 cgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAg                                                                                                 >  1:2560189/1‑150 (MQ=255)

                                                  gaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGt                                                                                                <  2:1114591/150‑1 (MQ=255)

                                                   aGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGtt                                                                                               <  2:8119859/150‑1 (MQ=255)

                                                    gTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTc                                                                                              <  1:8336624/150‑1 (MQ=255)

                                                     ttATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTcc                                                                                             >  1:1662680/1‑150 (MQ=255)

                                                      tATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCg                                                                                            >  1:5715630/1‑150 (MQ=255)

                                                        tCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑TAGGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCa                                                                                          >  1:3075382/1‑150 (MQ=255)

                                                           aCGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGc                                                                                       >  1:7933824/1‑150 (MQ=255)

                                                            cgcgCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGcc                                                                                      <  1:4041578/150‑1 (MQ=255)

                                                             gcgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCt                                                                                     >  1:4735402/1‑150 (MQ=255)

                                                              cgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTg                                                                                    >  2:7620691/1‑150 (MQ=255)

                                                               gcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTgg                                                                                   <  1:510135/150‑1 (MQ=255)

                                                                 cTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTgggg                                                                                 >  2:6011546/1‑150 (MQ=255)

                                                                 cTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTgggg                                                                                 <  2:7719901/150‑1 (MQ=255)

                                                                  tGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGa                                                                                <  1:9441638/150‑1 (MQ=255)

                                                                  tGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGa                                                                                >  1:6442369/1‑150 (MQ=255)

                                                                   gCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGAt                                                                               <  1:11038364/150‑1 (MQ=255)

                                                                     ggggAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATaa                                                                             <  1:1311666/150‑1 (MQ=255)

                                                                      gggAGGAGAAGGCAGGTCTATGCACACAGTACGTGCATGAACCTGTGGCAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAAc                                                                            <  2:5006603/150‑1 (MQ=255)

                                                                       ggaggaGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACg                                                                           <  1:6893617/150‑1 (MQ=255)

                                                                        gaggagAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGt                                                                          <  2:688927/150‑1 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                     <  2:1471346/150‑1 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                     >  2:318220/1‑150 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                   >  2:7044907/1‑150 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                   >  1:10501218/1‑150 (MQ=255)

                                                                                 gCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGc                                                                 >  1:7873113/1‑150 (MQ=255)

                                                                                  cAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGG‑A‑TGGGCGGGGGATCGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGcg                                                                >  2:11038950/1‑149 (MQ=255)

                                                                                    ggTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTgg                                                              >  1:4959637/1‑150 (MQ=255)

                                                                                          tGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAgg                                                        >  1:3535903/1‑150 (MQ=255)

                                                                                           gCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggc                                                       <  2:9639368/150‑1 (MQ=255)

                                                                                             acaAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATCGGAAGGAGTGCGAGTTTTTTCGCCAGGCTTGAGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggcgg                                                     >  1:5911126/1‑150 (MQ=255)

                                                                                             acaAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggcgg                                                     >  2:3839630/1‑150 (MQ=255)

                                                                                               aaaGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGgcggcg                                                   >  2:5917182/1‑150 (MQ=255)

                                                                                                 aGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATCGGAAGGATTGGGTGTTTTTTCGCCAGGCTTGGGTAGTTCCGCAACCCTGGGGAAAACGACACCCCGCATGGGGAAggcgcgccg                                                 >  1:8508958/1‑145 (MQ=255)

                                                                                                  gTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAg                                                <  1:341652/150‑1 (MQ=255)

                                                                                                    aCGTGCATGCACCTGTGGACAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGc                                              <  2:3306768/150‑1 (MQ=255)

                                                                                                      gTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTg                                            <  2:1031537/150‑1 (MQ=255)

                                                                                                       tGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTgc                                           >  1:805983/1‑150 (MQ=255)

                                                                                                           tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                       >  2:8088567/1‑150 (MQ=255)

                                                                                                             aaCCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAgg                                     >  1:4175644/1‑150 (MQ=255)

                                                                                                               ccTGTGGCACAGCCAGGGGGGGCACGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTATTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCg                                   <  1:8270184/150‑1 (MQ=255)

                                                                                                               ccTGTGGCAATGCCTGGGGTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATCGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATCACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCg                                   <  2:5713518/150‑1 (MQ=255)

                                                                                                                cTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCGGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGt                                  <  1:10698174/150‑1 (MQ=255)

                                                                                                                cTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGt                                  >  1:5957825/1‑150 (MQ=255)

                                                                                                                   tgGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGa                               >  2:4117776/1‑150 (MQ=255)

                                                                                                                    ggAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGaa                              >  1:1848393/1‑150 (MQ=255)

                                                                                                                     gAAAAGCCTGGGGTGGATCGCTGAGCGTCAGTAA‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGGTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAg                             <  2:944012/150‑1 (MQ=255)

                                                                                                                      aaaaGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCGGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGc                            >  1:1033710/1‑150 (MQ=255)

                                                                                                                       aaaGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCt                           <  2:5488067/150‑1 (MQ=255)

                                                                                                                           ccTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAg                       <  2:10077142/150‑1 (MQ=255)

                                                                                                                            cTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGa                      <  1:1174712/150‑1 (MQ=255)

                                                                                                                            cTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGa                      >  1:5063435/1‑150 (MQ=255)

                                                                                                                                ggTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCTGGCTGCGCCAGGCGTCGAAGCTGTAGACGGc                  >  2:3961445/1‑150 (MQ=255)

                                                                                                                                ggTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTAGCGGAGTTCCGCACCCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGGCGAAGCTGTAGACGGc                  >  2:2278609/1‑150 (MQ=255)

                                                                                                                                 gTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGcc                 >  2:365300/1‑150 (MQ=255)

                                                                                                                                 gTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGcc                 <  1:7340320/150‑1 (MQ=255)

                                                                                                                                 gTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGcc                 >  2:430147/1‑150 (MQ=255)

                                                                                                                                   ggATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAg               <  1:4314901/150‑1 (MQ=255)

                                                                                                                                     aTCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAggg             >  2:8771495/1‑150 (MQ=255)

                                                                                                                                        gCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGa          >  1:2657445/1‑150 (MQ=255)

                                                                                                                                          tGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGc        >  2:7675553/1‑150 (MQ=255)

                                                                                                                                           gAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGcc       >  1:989152/1‑150 (MQ=255)

CAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATG  >  NC_008463/558901‑559193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`