Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 41,753 T→C S207S (AGT→AGC)  tpsB2 → two‑partner secretion system transporter TpsB2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846341,7530TC99.8% 1574.1 / NA 631S207S (AGT→AGC) tpsB2two‑partner secretion system transporter TpsB2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (326/304);  minor base A (1/0);  total (327/304)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 631 total aligned reads displayed.

CCCGCGCGAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGTCGGGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAACCT  >  NC_008463/41606‑41899
                                                                                                                                                   |                                                                                                                                                  
 ccGCGCGAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCga                                                                                                                                                 >  1:9446020/1‑149 (MQ=21)
  cgcgcgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCgag                                                                                                                                                >  2:1489222/1‑148 (MQ=21)
  cgcgcgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCgag                                                                                                                                                >  2:8150976/1‑148 (MQ=21)
   gcgcgACCTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGt                                                                                                                                               <  2:751786/150‑1 (MQ=255)
    cgcgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCCGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTg                                                                                                                                              <  2:10113164/150‑1 (MQ=255)
    cgcgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTg                                                                                                                                              <  2:5713918/150‑1 (MQ=21)
      cgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCg                                                                                                                                            >  2:7960514/1‑150 (MQ=21)
          cTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGtctc                                                                                                                                        >  1:5144258/1‑150 (MQ=21)
             gCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAg                                                                                                                                     <  2:8392119/150‑1 (MQ=21)
             gCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAg                                                                                                                                     <  1:8488770/150‑1 (MQ=21)
                aTGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGc                                                                                                                                  >  1:1791563/1‑150 (MQ=21)
                    gCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAgc                                                                                                                              >  1:5613474/1‑150 (MQ=21)
                       ttCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGcgcg                                                                                                                           >  2:2108606/1‑150 (MQ=21)
                        tCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCACGGGCGAGCGCGa                                                                                                                          <  2:1646883/150‑1 (MQ=255)
                        tCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGa                                                                                                                          <  1:902493/150‑1 (MQ=21)
                         ccGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGAc                                                                                                                         <  1:6005491/150‑1 (MQ=21)
                          cGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACa                                                                                                                        <  1:6185048/150‑1 (MQ=21)
                           gggCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACaa                                                                                                                       >  1:3190602/1‑150 (MQ=21)
                                ggACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCt                                                                                                                  <  2:7273807/150‑1 (MQ=21)
                                ggACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCt                                                                                                                  <  2:6698893/150‑1 (MQ=21)
                                  aCCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTgg                                                                                                                <  1:8023917/150‑1 (MQ=21)
                                   ccGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGc                                                                                                               <  2:1977657/150‑1 (MQ=21)
                                      gCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCggg                                                                                                            >  1:3999058/1‑150 (MQ=21)
                                       cGAGCTGCTCGACCTGCGCGAACTGGAGCACCTGCTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGCGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGt                                                                                                           <  2:6086476/150‑1 (MQ=11)
                                       cGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGt                                                                                                           <  2:9535280/150‑1 (MQ=21)
                                          gctgctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGtctc                                                                                                        <  2:2677421/150‑1 (MQ=18)
                                            tgctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCg                                                                                                      <  1:7496380/150‑1 (MQ=17)
                                              ctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGGTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGcc                                                                                                    >  1:2458859/1‑150 (MQ=12)
                                                 gACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCAcg                                                                                                 >  1:8376140/1‑150 (MQ=17)
                                                      gcgcGAACTGGAGCACCTGGTCGACCCGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaa                                                                                            <  2:9662243/150‑1 (MQ=255)
                                                      gcgcGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaa                                                                                            >  1:8857645/1‑150 (MQ=17)
                                                       cgcgAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaac                                                                                           <  1:3076238/150‑1 (MQ=17)
                                                        gcgAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaaca                                                                                          >  2:8804481/1‑150 (MQ=17)
                                                         cgAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaacaa                                                                                         >  1:6909925/1‑150 (MQ=17)
                                                            aCTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacga                                                                                      <  2:8975335/150‑1 (MQ=17)
                                                              tGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgacg                                                                                    <  2:2312437/150‑1 (MQ=17)
                                                              tGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgacg                                                                                    <  1:4873717/150‑1 (MQ=17)
                                                              tGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgacg                                                                                    <  2:8549563/150‑1 (MQ=17)
                                                               ggAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACgg                                                                                   <  1:3374244/150‑1 (MQ=17)
                                                               ggAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACgg                                                                                   >  1:4110797/1‑150 (MQ=17)
                                                                 aGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCg                                                                                 <  1:6569679/150‑1 (MQ=17)
                                                                  gCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGa                                                                                >  1:5807422/1‑150 (MQ=17)
                                                                  gCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGa                                                                                <  2:6320000/150‑1 (MQ=17)
                                                                     aCTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGt                                                                             <  1:462702/150‑1 (MQ=17)
                                                                      cTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCCCGCGCACCAACGACGGCGACGTc                                                                            >  2:4235928/1‑150 (MQ=255)
                                                                      cTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTc                                                                            <  2:10371015/150‑1 (MQ=17)
                                                                       tGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCa                                                                           <  1:9877845/150‑1 (MQ=17)
                                                                       tGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCa                                                                           <  2:10883993/150‑1 (MQ=17)
                                                                         gTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGc                                                                         <  2:6389203/150‑1 (MQ=17)
                                                                           cGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGCGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCAc                                                                       <  2:6523504/150‑1 (MQ=17)
                                                                            gACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCAcc                                                                      <  1:368597/150‑1 (MQ=17)
                                                                             aCCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCg                                                                     >  1:116848/1‑150 (MQ=17)
                                                                              ccAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCgg                                                                    <  1:2964680/150‑1 (MQ=17)
                                                                              ccAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCgg                                                                    >  1:3836129/1‑150 (MQ=17)
                                                                                  ttGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGag                                                                <  2:7319907/150‑1 (MQ=17)
                                                                                   tGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGagc                                                               <  2:3623052/150‑1 (MQ=17)
                                                                                   tGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGagc                                                               <  2:3106473/150‑1 (MQ=17)
                                                                                         gCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGa                                                         <  1:8186907/150‑1 (MQ=17)
                                                                                         gCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGa                                                         <  2:3351705/150‑1 (MQ=17)
                                                                                           cTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATg                                                       <  1:2242008/150‑1 (MQ=17)
                                                                                            tGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATgg                                                      >  1:5077011/1‑150 (MQ=17)
                                                                                              ccGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGc                                                    <  2:2945739/150‑1 (MQ=17)
                                                                                                 tCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTg                                                 <  2:4987462/150‑1 (MQ=17)
                                                                                                  cgcgCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTgg                                                >  2:9194102/1‑150 (MQ=17)
                                                                                                   gcgcCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTggg                                               >  1:10744258/1‑150 (MQ=17)
                                                                                                      ccAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCt                                            >  1:1633726/1‑150 (MQ=21)
                                                                                                      ccAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCt                                            <  1:10408960/150‑1 (MQ=21)
                                                                                                      ccAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCt                                            <  2:7886349/150‑1 (MQ=21)
                                                                                                         ggCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGa                                         >  1:10234448/1‑150 (MQ=21)
                                                                                                           cgcAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACt                                       >  1:529041/1‑150 (MQ=21)
                                                                                                              aaCTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTggg                                    <  2:3214842/150‑1 (MQ=21)
                                                                                                               aCTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGa                                   >  2:2557488/1‑150 (MQ=21)
                                                                                                               aCTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGa                                   >  2:1297420/1‑150 (MQ=21)
                                                                                                                   gAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGc                               >  1:5463997/1‑150 (MQ=21)
                                                                                                                    aGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGcc                              >  2:7061823/1‑150 (MQ=21)
                                                                                                                     gCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGccc                             >  2:5088266/1‑150 (MQ=21)
                                                                                                                      cTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCg                            <  2:4728868/150‑1 (MQ=21)
                                                                                                                       tAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGc                           <  1:2793169/150‑1 (MQ=18)
                                                                                                                        aGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCt                          >  1:11096750/1‑150 (MQ=17)
                                                                                                                          tGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTgg                        <  1:10628515/150‑1 (MQ=17)
                                                                                                                               ggCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTg                   <  2:4556574/150‑1 (MQ=17)
                                                                                                                               ggCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTg                   <  1:10008799/150‑1 (MQ=17)
                                                                                                                               ggCAGCGAGGTCGGCGGCAGCCGAGTGCGTATCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTg                   <  2:4081286/150‑1 (MQ=12)
                                                                                                                                 cAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGc                 <  1:7904176/150‑1 (MQ=17)
                                                                                                                                  aGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGcc                >  2:8920900/1‑150 (MQ=17)
                                                                                                                                   gCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCg               >  1:10095409/1‑150 (MQ=17)
                                                                                                                                   gCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCg               >  1:9627105/1‑150 (MQ=17)
                                                                                                                                     gAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGAc             >  2:1129932/1‑150 (MQ=17)
                                                                                                                                     gAGGTCGGCGGCAGCCGAGAGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGCCGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGAc             <  1:6316547/150‑1 (MQ=255)
                                                                                                                                        gTCGGCGGCAGCCGAGCGCGTCTCACGCGCGAGCGCCCCACGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAg          <  2:5577846/150‑1 (MQ=11)
                                                                                                                                           ggcggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTc       >  2:6778295/1‑150 (MQ=17)
                                                                                                                                            gcggcAGCCGAGTGCGTCTCAAGGGCGAGCGCTACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCATCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCa      >  2:5300050/1‑150 (MQ=255)
                                                                                                                                             cggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGCCGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCaa     <  2:9829254/150‑1 (MQ=12)
                                                                                                                                             cggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCaa     <  1:7120821/150‑1 (MQ=17)
                                                                                                                                             cggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCaa     >  1:445331/1‑150 (MQ=17)
                                                                                                                                               gcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAAcc   >  2:5847923/1‑150 (MQ=17)
                                                                                                                                               gcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAAcc   >  2:10483931/1‑150 (MQ=17)
                                                                                                                                               gcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAAcc   <  1:2002597/150‑1 (MQ=17)
                                                                                                                                               gcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCGGGCGGGTCTCCGCCACGCGCAACATCGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAAcc   <  2:10810486/150‑1 (MQ=255)
                                                                                                                                                cAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAACCt  <  1:3519487/150‑1 (MQ=17)
                                                                                                                                                   |                                                                                                                                                  
CCCGCGCGAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGTCGGGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAACCT  >  NC_008463/41606‑41899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑