Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,543,6620AG99.8% 2192.7 / NA 562T494A (ACG→GCG) PA14_RS16110PucR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (213/348);  minor base C (0/1);  total (213/349)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 562 total aligned reads displayed.

AACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCG  >  NC_008463/3543515‑3543808
                                                                                                                                                   |                                                                                                                                                  
 aCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGa                                                                                                                                                 <  1:5148613/150‑1 (MQ=255)
 aCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGa                                                                                                                                                 <  2:3723915/150‑1 (MQ=255)
 aCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGa                                                                                                                                                 <  1:2538020/150‑1 (MQ=255)
 aCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGa                                                                                                                                                 <  2:6682249/150‑1 (MQ=255)
     cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGAGGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgggg                                                                                                                                             >  2:8607400/1‑150 (MQ=255)
         cAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                         >  1:7771449/1‑150 (MQ=255)
          cgCGGCTACCCCTCCACCCCCAGAGCCTGGTCTACCGGCTACGCCGCCTCGAGGAAATCAGCGGTCGCCCGCTGGACACCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGa                                                                                                                                        <  2:9607676/149‑1 (MQ=255)
            cGGCGACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCAGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGGCGCCGGAAGCGAGGGGCAGGACg                                                                                                                                      <  1:2114638/150‑1 (MQ=255)
              gCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGc                                                                                                                                    >  1:9961954/1‑150 (MQ=255)
                tacacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGa                                                                                                                                  >  2:10137768/1‑150 (MQ=255)
                 acacatacccaCCCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCCGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGaa                                                                                                                                 <  2:2504624/150‑1 (MQ=255)
                  cacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAag                                                                                                                                >  2:6878608/1‑150 (MQ=255)
                      tacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                            <  1:7614944/150‑1 (MQ=255)
                          cacgCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcct                                                                                                                        <  2:5542360/147‑1 (MQ=255)
                          caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGaa                                                                                                                                 >  1:10909504/1‑141 (MQ=255)
                          caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcct                                                                                                                        <  2:9100156/150‑1 (MQ=255)
                             gCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                     <  1:9085623/150‑1 (MQ=255)
                               agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGCTGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                   <  2:1617227/150‑1 (MQ=255)
                                  gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGc                                                                                                                <  2:9718766/150‑1 (MQ=255)
                                   ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               <  1:6858276/150‑1 (MQ=255)
                                   ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               <  2:9187752/150‑1 (MQ=255)
                                   ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               <  1:4721099/150‑1 (MQ=255)
                                   ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCGGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               <  1:8976838/150‑1 (MQ=255)
                                     tGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCCGt                                                                                                             >  2:8910148/1‑150 (MQ=255)
                                       gTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTcc                                                                                                           <  2:1769462/150‑1 (MQ=255)
                                           aCCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCGGGAGCAGTCCgcgc                                                                                                       >  2:3619150/1‑150 (MQ=255)
                                            ccGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCa                                                                                                      >  1:9762745/1‑150 (MQ=255)
                                              ggATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAcc                                                                                                    <  2:1068879/150‑1 (MQ=255)
                                                aTACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCAt                                                                                                  <  1:10275259/150‑1 (MQ=255)
                                                aTACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCAt                                                                                                  <  1:2667000/150‑1 (MQ=255)
                                                  aCGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATgg                                                                                                >  2:9327618/1‑150 (MQ=255)
                                                    gccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcg                                                                                              >  2:7278927/1‑150 (MQ=255)
                                                    gccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcg                                                                                              <  2:7032250/150‑1 (MQ=255)
                                                     ccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcgg                                                                                             >  2:8242482/1‑150 (MQ=255)
                                                     ccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcgg                                                                                             >  1:8157438/1‑150 (MQ=255)
                                                         aTCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGAc                                                                                         <  2:3932874/150‑1 (MQ=255)
                                                            gAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATg                                                                                      <  1:1903032/150‑1 (MQ=255)
                                                             aGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATgg                                                                                     >  2:1270347/1‑150 (MQ=255)
                                                                 aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTg                                                                                 >  1:3020669/1‑150 (MQ=255)
                                                                  aTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                <  2:4106107/150‑1 (MQ=255)
                                                                  aTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                >  1:7235028/1‑150 (MQ=255)
                                                                  aTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGGTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                <  1:1019452/150‑1 (MQ=255)
                                                                   gcAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGTAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGTCTGAc                                                                               >  2:4501534/2‑150 (MQ=255)
                                                                    cAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                              <  2:10541511/150‑1 (MQ=255)
                                                                      gCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcggc                                                                            >  1:10065577/1‑150 (MQ=255)
                                                                      gCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcggc                                                                            <  1:8683261/150‑1 (MQ=255)
                                                                         gTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                         <  2:5861490/150‑1 (MQ=255)
                                                                          tcgctcgcTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAg                                                                        <  1:3931339/150‑1 (MQ=255)
                                                                           cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCACCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGc                                                                       <  2:1691778/150‑1 (MQ=255)
                                                                            gctcgctGGACAGCACCGCCGATGTGGCGCCCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                      <  2:7434887/150‑1 (MQ=255)
                                                                             ctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                     >  1:8287988/1‑150 (MQ=255)
                                                                                 ctGGACCGCACCGCCGATGTGGCGACCCTCTGGGTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGCGGGGCCGGCCGGCGAAGAGCAGCCTCCTGGAGCAGTGCGCGCGCCATGGCGGCGACAGGGACTGACGGCGGAGCCCggtc                                                                 >  2:9870659/1‑147 (MQ=255)
                                                                                 ctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATa                                                                 <  1:6704510/150‑1 (MQ=255)
                                                                                 ctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATa                                                                 <  1:3525214/150‑1 (MQ=255)
                                                                                  tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGCCGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAc                                                                >  2:1966388/1‑150 (MQ=255)
                                                                                    gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCt                                                              <  1:9284414/150‑1 (MQ=255)
                                                                                    gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCt                                                              <  1:7746940/150‑1 (MQ=255)
                                                                                    gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCt                                                              <  1:6354406/150‑1 (MQ=255)
                                                                                        gCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                          >  1:10803855/1‑150 (MQ=255)
                                                                                           ccgccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCtt                                                       <  1:10011597/150‑1 (MQ=255)
                                                                                            cgccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTg                                                      <  2:10896317/150‑1 (MQ=255)
                                                                                            cgccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTg                                                      <  2:479273/150‑1 (MQ=255)
                                                                                               cgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAg                                                   <  1:6509935/150‑1 (MQ=255)
                                                                                               cgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCGGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATCCCTGGCGCTTGCAg                                                   <  2:3400503/150‑1 (MQ=255)
                                                                                                gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGTCATGGACTGACGGCGGAGCCCGATACCTGGCGCGTGCAgg                                                  >  2:220572/1‑150 (MQ=255)
                                                                                                gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAgg                                                  >  2:4925782/1‑150 (MQ=255)
                                                                                                   gtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacg                                               >  1:10635230/1‑150 (MQ=255)
                                                                                                        gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCTCCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgcc                                          >  2:8827764/1‑150 (MQ=255)
                                                                                                         aCCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                         >  2:1121239/1‑150 (MQ=255)
                                                                                                           ccacttgTTCGCCTTGCACGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                       <  2:8351034/144‑1 (MQ=255)
                                                                                                             tctGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgccgc                                     <  1:3651892/150‑1 (MQ=255)
                                                                                                                ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCaa                                  <  1:5463369/150‑1 (MQ=255)
                                                                                                                  ttCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACa                                <  1:1542602/150‑1 (MQ=255)
                                                                                                                  ttCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACa                                <  1:3286938/150‑1 (MQ=255)
                                                                                                                   tCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAg                               <  2:6223174/150‑1 (MQ=255)
                                                                                                                    cGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAgg                              <  1:2390487/150‑1 (MQ=255)
                                                                                                                      ccTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcg                            <  1:3797613/150‑1 (MQ=255)
                                                                                                                       cTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcgc                           <  2:5385689/150‑1 (MQ=255)
                                                                                                                        ttGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGcgcg                          <  1:7565458/150‑1 (MQ=255)
                                                                                                                          gCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGc                        <  1:2049895/150‑1 (MQ=255)
                                                                                                                           cAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGcc                       >  1:8863239/1‑150 (MQ=255)
                                                                                                                            aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCa                      <  2:3885491/150‑1 (MQ=255)
                                                                                                                            aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCa                      >  2:2675322/1‑150 (MQ=255)
                                                                                                                             ggCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                     <  1:313066/150‑1 (MQ=255)
                                                                                                                             ggCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                     <  2:773075/150‑1 (MQ=255)
                                                                                                                              gCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAgg                    <  1:4820347/150‑1 (MQ=255)
                                                                                                                               cAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGa                   <  2:4309234/150‑1 (MQ=255)
                                                                                                                                 aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                 <  1:8531088/150‑1 (MQ=255)
                                                                                                                                 aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                 >  2:805081/1‑150 (MQ=255)
                                                                                                                                  cccGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                >  2:6587065/1‑150 (MQ=255)
                                                                                                                                  cccGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                <  1:6034191/150‑1 (MQ=255)
                                                                                                                                     ggCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGNAGTCCGCGCACCATGGCGGCGACTTGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCCACAGGCGCGGCCAGGAACGGtt             >  1:5799038/1‑150 (MQ=255)
                                                                                                                                     ggCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGtt             <  1:4498355/150‑1 (MQ=255)
                                                                                                                                      gCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTg            <  2:3060317/150‑1 (MQ=255)
                                                                                                                                            gCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGc      <  2:7551178/150‑1 (MQ=255)
                                                                                                                                              cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCCGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcg    >  1:1185514/1‑150 (MQ=255)
                                                                                                                                              cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcg    <  1:9604215/150‑1 (MQ=255)
                                                                                                                                              cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcg    >  1:5658407/1‑150 (MQ=255)
                                                                                                                                               ggAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgc   <  2:6425719/150‑1 (MQ=255)
                                                                                                                                               ggAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgc   >  1:7055007/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCG  >  NC_008463/3543515‑3543808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑