BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,566,769	A→G	pseudogene (1120/1275 nt)	PA14_RS16215 →	fatty acid desaturase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,566,769	0	A	G	100.0%	1753.4 / NA	452	pseudogene (1120/1275 nt)	PA14_RS16215	fatty acid desaturase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (245/207); total (245/207)

Only 100 of 457 total aligned reads displayed.

GTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGC  >  NC_008463/3566621‑3566914

gtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                                                                   >  1:6193491/1‑150 (MQ=255)

 ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAAc                                                                                                                                                  <  2:297671/150‑1 (MQ=255)

  tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                                                                 >  2:9367091/1‑150 (MQ=255)

   ctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                                                                <  2:4824048/150‑1 (MQ=255)

     ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTcc                                                                                                                                              >  1:11008774/1‑150 (MQ=255)

        tCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                                                           >  2:4672563/1‑150 (MQ=255)

        tCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                                                           >  1:10690889/1‑150 (MQ=255)

          aTGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGACCTTCCTGATc                                                                                                                                         >  1:8138786/1‑150 (MQ=255)

          aTGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATc                                                                                                                                         >  2:2455731/1‑150 (MQ=255)

            gCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCt                                                                                                                                       <  1:5764170/150‑1 (MQ=255)

             cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTg                                                                                                                                      <  1:9496105/150‑1 (MQ=255)

             cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTg                                                                                                                                      <  1:10513649/150‑1 (MQ=255)

             cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTg                                                                                                                                      <  1:5655884/150‑1 (MQ=255)

             cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTg                                                                                                                                      >  2:9208264/1‑150 (MQ=255)

              aGGAGCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGTGGGTGAAATTCTGGCGCTTCGCGCCGGAGCGCGCGGCCATCGAGGCGCGCCCATCGTAGTTCGGCCCGATGACAGAGACCTGGGGCATGTGGGAGAAGTTCCTGATCCTgc                                                                                                                                     <  2:7033814/150‑1 (MQ=255)

                gACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgc                                                                                                                                   <  1:3391849/150‑1 (MQ=255)

                      cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                             >  2:5338171/1‑150 (MQ=255)

                        cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGTTGAACTTCTGGCGCAACGCGCCGGAGCGCGCGGCCAACGTGGCGCGCCCCACGAAGTTCGGCCCGATGACCGATACATGGGGCATGTGGGAGAACTTCCTGAACCTGCGCGaccacag                                                                                                                           >  1:8669871/1‑148 (MQ=255)

                        cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACct                                                                                                                           >  1:1028191/1‑150 (MQ=255)

                        cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACct                                                                                                                           <  2:2374842/150‑1 (MQ=255)

                         aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                          <  2:6204495/150‑1 (MQ=255)

                         aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                          >  2:728536/1‑150 (MQ=255)

                         aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                          >  1:10087000/1‑150 (MQ=255)

                          gCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctct                                                                                                                         <  2:8061390/150‑1 (MQ=255)

                          gCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctct                                                                                                                         <  1:7180713/150‑1 (MQ=255)

                           ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCCACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCCTGTGGGAGACCTTCCTGATCCTGCGCGACCACCTCTg                                                                                                                        >  2:9116532/1‑150 (MQ=255)

                           ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTg                                                                                                                        >  1:4794818/1‑150 (MQ=255)

                            cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGc                                                                                                                       <  1:8794628/150‑1 (MQ=255)

                            cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGc                                                                                                                       >  1:5614198/1‑150 (MQ=255)

                             aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                      <  1:143625/150‑1 (MQ=255)

                             aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                      <  1:10899371/150‑1 (MQ=255)

                             aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                      <  1:2502085/150‑1 (MQ=255)

                              cGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcg                                                                                                                     >  1:7429049/1‑150 (MQ=255)

                                aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                   >  2:4225956/1‑150 (MQ=255)

                                aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                   >  1:6676258/1‑150 (MQ=255)

                                 cTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCgg                                                                                                                  >  2:2190605/1‑150 (MQ=255)

                                  ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                                                                 >  1:7821580/1‑150 (MQ=255)

                                   tCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                                                                >  2:6883941/1‑150 (MQ=255)

                                   tCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                                                                >  1:7051958/1‑150 (MQ=255)

                                   tCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                                                                <  1:7533061/150‑1 (MQ=255)

                                    ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                                                               <  2:6101432/150‑1 (MQ=255)

                                    ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                                                               >  2:3110729/1‑150 (MQ=255)

                                     cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                                                                              >  2:1925095/1‑150 (MQ=255)

                                       accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                                                                             >  2:3975457/5‑150 (MQ=255)

                                       accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                                                                             >  2:3609947/5‑150 (MQ=255)

                                           ccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGa                                                                                                         >  1:1562777/1‑150 (MQ=255)

                                             gCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                                                                       <  1:3776455/150‑1 (MQ=255)

                                               aaGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGAGCGCGGCAATCGAGTGGAGGACCTCTTACTACTGCCCGAGGACAGAGGCCTGGGCCATGGGCCAGAACTGCATGATCCTCCGCGACGTCCTCGGCGGCGGCCAGTCGTAccag                                                                                                     >  2:2720692/1‑148 (MQ=255)

                                                 gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                   <  1:9008658/150‑1 (MQ=255)

                                                 gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                   <  1:3164261/150‑1 (MQ=255)

                                                 gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                   <  2:10779268/150‑1 (MQ=255)

                                                   cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCg                                                                                                 <  1:2302269/150‑1 (MQ=255)

                                                    gggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                >  2:1628956/1‑150 (MQ=255)

                                                     ggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑a                                                                                              >  1:2064950/1‑150 (MQ=255)

                                                       ggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                                                            >  2:5568045/1‑148 (MQ=255)

                                                        gTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatc                                                                                           >  2:6808460/1‑147 (MQ=255)

                                                           aaCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑tcgtc                                                                                        >  1:2793196/1‑150 (MQ=255)

                                                              ttCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGAc                                                                                     <  2:5775583/150‑1 (MQ=255)

                                                                       aTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACcgc                                                                            >  1:9818811/1‑150 (MQ=255)

                                                                          gcgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ACTCGTCGACTGGTACCGCgag                                                                         >  2:3323105/1‑150 (MQ=255)

                                                                             ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                      >  1:1882587/1‑150 (MQ=255)

                                                                             ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                      >  2:6706444/1‑150 (MQ=255)

                                                                             ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                      >  2:784489/1‑150 (MQ=255)

                                                                              cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                     >  1:9283351/1‑150 (MQ=255)

                                                                                gAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCAt                                                                   <  1:4902653/150‑1 (MQ=255)

                                                                                       cgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgc                                                            <  2:7669704/150‑1 (MQ=255)

                                                                                         gCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                                                          <  2:8601032/150‑1 (MQ=255)

                                                                                              cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTcc                                                     >  2:6552377/3‑150 (MQ=255)

                                                                                              cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTcc                                                     <  1:10203088/148‑1 (MQ=255)

                                                                                              cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTcc                                                     <  1:7325750/148‑1 (MQ=255)

                                                                                                      gcCCCCCGAAGTTCGGCCCGCCGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCg                                             <  1:6512208/150‑1 (MQ=255)

                                                                                                       ccccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGa                                            <  2:8830343/150‑1 (MQ=255)

                                                                                                        cccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGag                                           >  1:9180800/1‑149 (MQ=255)

                                                                                                              aaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccgcc                                     >  1:6122977/1‑150 (MQ=255)

                                                                                                                gTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCaa                                   <  2:3530001/150‑1 (MQ=255)

                                                                                                                gTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCaa                                   >  1:1119043/1‑150 (MQ=255)

                                                                                                                   cGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCCGTCGGACCCGTTCGGAA‑TCGACGACTGGAACCGCGAGAACCCTGCCCGGCTCGCGCCGGAGACCGATGCCGCCAACCa                                >  2:7744441/1‑150 (MQ=255)

                                                                                                                    ggCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCGGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAg                               <  2:9557154/150‑1 (MQ=255)

                                                                                                                     gCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGc                              <  1:8087124/150‑1 (MQ=255)

                                                                                                                     gCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGc                              >  1:6732622/1‑150 (MQ=255)

                                                                                                                      cccGATGACCGAGACCTGGGGCATGTGGGAGTACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGGCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAGCGATGCCGCCAACCAGcc                             >  2:11010325/1‑150 (MQ=255)

                                                                                                                      cccGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGcc                             <  1:7660546/150‑1 (MQ=255)

                                                                                                                        cGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGcccc                           <  2:975463/150‑1 (MQ=255)

                                                                                                                         gATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCCGCCCca                          <  1:5170960/150‑1 (MQ=255)

                                                                                                                         gATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCca                          >  2:7469869/1‑150 (MQ=255)

                                                                                                                            gACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCacac                       <  1:4523991/150‑1 (MQ=255)

                                                                                                                              ccgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACacca                     >  1:2953339/6‑150 (MQ=255)

                                                                                                                               cgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaa                    >  2:1249731/5‑150 (MQ=255)

                                                                                                                               cgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaa                    <  1:4007971/146‑1 (MQ=255)

                                                                                                                                gagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaac                   >  2:5132186/4‑150 (MQ=255)

                                                                                                                                    ccTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac               >  2:10627820/1‑150 (MQ=255)

                                                                                                                                    ccTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac               <  1:10438207/150‑1 (MQ=255)

                                                                                                                                      tGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAAccc             >  2:4533515/1‑150 (MQ=255)

                                                                                                                                       tggtgaTGTGGTAGACCTTCCTGATCCTCCGCGAGCACCTCTGCCGCGGCCTGTCGGACCGGTTCGGAA‑TCGTCGACTTGTACCGCGAGAACCATGCCCGGATCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCg            <  2:10198423/145‑1 (MQ=255)

                                                                                                                                         ggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCa          <  1:4216222/150‑1 (MQ=255)

                                                                                                                                         ggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCa          >  2:2595759/1‑150 (MQ=255)

                                                                                                                                         ggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCa          <  2:3911218/150‑1 (MQ=255)

                                                                                                                                           cATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATc        <  1:535660/150‑1 (MQ=255)

                                                                                                                                                gggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAgg   <  2:8700851/150‑1 (MQ=255)

                                                                                                                                                 ggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGc  <  1:8467466/150‑1 (MQ=255)

GTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGC  >  NC_008463/3566621‑3566914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`