BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,566,788	A→G	pseudogene (1139/1275 nt)	PA14_RS16215 →	fatty acid desaturase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,566,788	0	A	G	100.0%	1696.1 / NA	439	pseudogene (1139/1275 nt)	PA14_RS16215	fatty acid desaturase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (232/207); total (232/207)

Only 100 of 442 total aligned reads displayed.

CTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATCACACAGG  >  NC_008463/3566640‑3566933

   cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGTAGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                                                >  2:6173111/1‑150 (MQ=255)

   cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGACCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCCTGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                                                >  2:925131/1‑150 (MQ=255)

   cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGTACTTCCTGATCCTGCGCGaccac                                                                                                                                                >  2:2602856/1‑150 (MQ=255)

   cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                                                <  2:9518568/150‑1 (MQ=255)

    ccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccacc                                                                                                                                               <  1:6135832/150‑1 (MQ=255)

      aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                                             <  2:204581/150‑1 (MQ=255)

      aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                                             >  1:10087000/1‑150 (MQ=255)

       gCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctct                                                                                                                                            <  2:8061390/150‑1 (MQ=255)

          aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                                         <  1:143625/150‑1 (MQ=255)

          aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                                         <  1:10899371/150‑1 (MQ=255)

          aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                                         <  1:2502085/150‑1 (MQ=255)

           cGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcg                                                                                                                                        >  1:7430036/1‑150 (MQ=255)

            gACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgc                                                                                                                                       >  2:5612205/1‑150 (MQ=255)

             aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                                      >  1:294075/1‑150 (MQ=255)

             aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                                      >  1:1163943/1‑150 (MQ=255)

               ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                                                                                    >  1:7821580/1‑150 (MQ=255)

                 ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                                                                                  >  2:3110729/1‑150 (MQ=255)

                 ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCATGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                                                                                  >  1:4225003/1‑150 (MQ=255)

                  cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                                                                                                 <  1:4106913/150‑1 (MQ=255)

                       accGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGTGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCgg                                                                                                                             >  2:5289153/2‑150 (MQ=255)

                        ccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGa                                                                                                                            >  1:1562777/1‑150 (MQ=255)

                          gCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                                                                                          <  1:3776455/150‑1 (MQ=255)

                              gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                                      <  2:10777399/150‑1 (MQ=255)

                               ccGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTc                                                                                                                     <  2:6631749/150‑1 (MQ=255)

                                 gggggTGAACGTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCCCCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                                   >  2:1912356/1‑150 (MQ=255)

                                  ggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑a                                                                                                                 >  1:8902575/1‑150 (MQ=255)

                                       gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatcgt                                                                                                            <  2:626121/150‑6 (MQ=255)

                                        aaCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑tcgtc                                                                                                           >  2:5464612/1‑150 (MQ=255)

                                           ttCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGAc                                                                                                        <  2:6834409/150‑1 (MQ=255)

                                            tCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGGTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ACTCGTCGACt                                                                                                       >  2:9183786/1‑150 (MQ=255)

                                             cTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCCGTCGGACCCGGGCGGAA‑TCGTCGACTg                                                                                                      >  2:3787154/1‑150 (MQ=255)

                                                  gcATCGCGCCGGAGCGCGCGGCCAACGACGCGCGCCCCTCGAAGTTCGGCCCGATGTCCTAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTaac                                                                                                 <  2:2431156/150‑3 (MQ=255)

                                                   cATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTCCcg                                                                                                >  2:5140745/1‑150 (MQ=255)

                                                      cgcgCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCga                                                                                             >  1:9128605/1‑150 (MQ=255)

                                                       gcgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ACTCGTCGACTGGTACCGCgag                                                                                            >  2:3323105/1‑150 (MQ=255)

                                                          ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCgagacc                                                                                         >  2:8409905/1‑148 (MQ=255)

                                                          ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                                         >  1:1882587/1‑150 (MQ=255)

                                                           cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                                        >  1:9283351/1‑150 (MQ=255)

                                                           cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                                        >  2:2799337/1‑150 (MQ=255)

                                                           cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                                        >  1:8186228/1‑150 (MQ=255)

                                                             gAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCAt                                                                                      <  1:4902653/150‑1 (MQ=255)

                                                             gAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCAt                                                                                      >  2:10588469/1‑150 (MQ=255)

                                                                cgcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGcc                                                                                   >  1:9311840/1‑150 (MQ=255)

                                                                cgcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGcc                                                                                   <  1:9624178/150‑1 (MQ=255)

                                                                     ggCCAACGAGGCGCGCCCCTCGACGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgct                                                                              <  1:8481120/150‑1 (MQ=255)

                                                                      gCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                                                                             >  2:6772431/1‑150 (MQ=255)

                                                                        caacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgc                                                                           <  2:46781/150‑1 (MQ=255)

                                                                        caacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgc                                                                           <  1:5713031/150‑1 (MQ=255)

                                                                           cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTcc                                                                        >  2:6552377/3‑150 (MQ=255)

                                                                           cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTcc                                                                        <  1:10203088/148‑1 (MQ=255)

                                                                               gcgcgcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGgag                                                                    <  1:2246807/150‑1 (MQ=255)

                                                                                   gcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCg                                                                >  2:2273663/1‑150 (MQ=255)

                                                                                   gcCCCCCGAAGTTCGGCCCGCCGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCg                                                                <  1:6512208/150‑1 (MQ=255)

                                                                                    ccccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGa                                                               <  1:7213115/150‑1 (MQ=255)

                                                                                     cccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGGTCGGAA‑TCGTCGACTGGGACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGAt                                                              >  1:8716845/1‑150 (MQ=255)

                                                                                       cTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                                            <  1:5632521/150‑1 (MQ=255)

                                                                                       cTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                                            >  1:11061889/1‑150 (MQ=255)

                                                                                        tcgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgcc                                                           >  1:8097629/4‑150 (MQ=255)

                                                                                                cGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCCGTCGGACCCGTTCGGAA‑TCGACGACTGGAACCGCGAGAACCCTGCCCGGCTCGCGCCGGAGACCGATGCCGCCAACCa                                                   >  2:7744441/1‑150 (MQ=255)

                                                                                                 ggCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAg                                                  <  2:764325/150‑1 (MQ=255)

                                                                                                  gCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGc                                                 >  1:5985318/1‑150 (MQ=255)

                                                                                                  gCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGc                                                 >  1:203023/1‑150 (MQ=255)

                                                                                                   cccGATGACCGAGACCTGGGGCATGTGGGAGTACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGGCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAGCGATGCCGCCAACCAGcc                                                >  2:11010325/1‑150 (MQ=255)

                                                                                                   cccGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGcc                                                <  1:10609638/150‑1 (MQ=255)

                                                                                                      gATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCCGCCCca                                             <  1:5170960/150‑1 (MQ=255)

                                                                                                      gATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCca                                             <  1:3061861/150‑1 (MQ=255)

                                                                                                         gACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCacac                                          <  2:419893/150‑1 (MQ=255)

                                                                                                         gACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCacac                                          >  2:4389403/1‑150 (MQ=255)

                                                                                                          accgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACacc                                         >  2:9624127/7‑150 (MQ=255)

                                                                                                            cgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaa                                       >  2:1249731/5‑150 (MQ=255)

                                                                                                              agaCCTGGGGCATGTGGGAGAACTTCCAGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaacc                                     >  2:9001338/3‑150 (MQ=255)

                                                                                                                 ccTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac                                  <  2:8798708/150‑1 (MQ=255)

                                                                                                                 ccTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac                                  <  1:766365/150‑1 (MQ=255)

                                                                                                                 ccTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac                                  <  1:10438207/150‑1 (MQ=255)

                                                                                                                  cTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCaaccaacc                                 >  1:2692763/1‑150 (MQ=255)

                                                                                                                  cTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGAACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCaaccaacc                                 >  2:3599506/1‑150 (MQ=255)

                                                                                                                   tGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAAccc                                >  2:7812397/1‑150 (MQ=255)

                                                                                                                   tGGGGCATGTGGGAGAACGTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCAGACAACCAGCCCCACACCAAGCAAccc                                <  2:10398830/150‑1 (MQ=255)

                                                                                                                      ggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCa                             <  2:3911407/150‑1 (MQ=255)

                                                                                                                        cATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATc                           <  1:535660/150‑1 (MQ=255)

                                                                                                                           gtgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCa                        <  1:7540003/150‑1 (MQ=255)

                                                                                                                            tgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAg                       >  2:8636753/1‑150 (MQ=255)

                                                                                                                             gggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTCCCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAgg                      >  1:3313936/1‑150 (MQ=255)

                                                                                                                              ggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGc                     <  1:6700096/150‑1 (MQ=255)

                                                                                                                              ggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGc                     <  1:1026178/150‑1 (MQ=255)

                                                                                                                               gagaaCTTCCCGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑ACGTCGACGGGTCCCGCGAGAACCAGGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCACCCAACCCGCATCCCAGGCt                    <  2:4915651/147‑1 (MQ=255)

                                                                                                                                agaaCTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTa                   <  2:1477020/148‑1 (MQ=255)

                                                                                                                                     ttCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGcc              >  2:709861/1‑150 (MQ=255)

                                                                                                                                     ttCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGcc              <  1:7024774/150‑1 (MQ=255)

                                                                                                                                     ttCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGcc              <  1:7350164/150‑1 (MQ=255)

                                                                                                                                     ttCCTGAACCAGCGCGACCACCACTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCACCCAACCCGCATCCCAGGCTACCGcc              <  1:3817402/150‑1 (MQ=255)

                                                                                                                                       ccTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTg            <  1:7151890/150‑1 (MQ=255)

                                                                                                                                         tGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGAt          >  2:7018072/1‑150 (MQ=255)

                                                                                                                                         tGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGAt          <  2:5880501/150‑1 (MQ=255)

                                                                                                                                         tGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGAt          >  1:4528170/1‑150 (MQ=255)

                                                                                                                                          gATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATc         >  2:25124/1‑150 (MQ=255)

                                                                                                                                             ccTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATcaca      <  1:4755417/150‑1 (MQ=255)

                                                                                                                                                gcgcGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATCACACAg   >  2:7388009/1‑150 (MQ=255)

                                                                                                                                                gcgcGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATCACACAg   >  1:8771058/1‑150 (MQ=255)

                                                                                                                                                gcgcGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATCACACAg   >  1:9996444/1‑150 (MQ=255)

CTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCTACCGCCTGATCACACAGG  >  NC_008463/3566640‑3566933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`