Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,711,791 G→C V51L (GTG→CTG)  cobA → uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,711,7910GC100.0% 1938.9 / NA 502V51L (GTG→CTG) cobAuroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (268/234);  total (268/234)

Only 100 of 505 total aligned reads displayed.

AGCGGTAAGGTCTGGCTGGTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATTTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGGTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCGAAGAA  >  NC_008463/3711644‑3711937
                                                                                                                                                   |                                                                                                                                                  
    gTAAGGTCTGGCTGGTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTcc                                                                                                                                              >  2:2593144/1‑150 (MQ=255)
         gTCTGGCTGGTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTc                                                                                                                                         <  1:1328186/150‑1 (MQ=255)
         gTCTGGCTGGTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTc                                                                                                                                         <  2:1424825/150‑1 (MQ=255)
            tggctggTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGc                                                                                                                                      >  1:2463285/1‑150 (MQ=255)
             ggctggTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCa                                                                                                                                     <  1:7720604/150‑1 (MQ=255)
              gctggTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCaa                                                                                                                                    >  2:10845616/1‑150 (MQ=255)
               ctggTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCcaa                                                                                                                                   >  2:6624430/1‑150 (MQ=255)
                 ggtgggtgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAAcg                                                                                                                                 >  2:8280076/1‑150 (MQ=255)
                  gtgggtgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAAcgc                                                                                                                                <  1:10068349/150‑1 (MQ=255)
                  gtgggtgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAAcgc                                                                                                                                >  1:1137603/1‑150 (MQ=255)
                     ggtgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACgcggc                                                                                                                             <  2:2392418/150‑1 (MQ=255)
                     ggtgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACgcggc                                                                                                                             <  1:3999112/150‑1 (MQ=255)
                       tgCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGcggcgg                                                                                                                           >  2:9758966/1‑150 (MQ=255)
                        gCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCggcggc                                                                                                                          <  2:7904910/150‑1 (MQ=255)
                           ggTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGc                                                                                                                       <  2:5814012/150‑1 (MQ=255)
                           ggTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGGTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGc                                                                                                                       <  1:2799731/150‑1 (MQ=255)
                             tCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCg                                                                                                                     <  1:9017465/150‑1 (MQ=255)
                               ccGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCt                                                                                                                   <  1:7066053/150‑1 (MQ=255)
                                     aTCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCacac                                                                                                             >  1:4241199/1‑150 (MQ=255)
                                      tCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACAcc                                                                                                            >  2:1850642/1‑150 (MQ=255)
                                          gAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAATCGCGGCGGCTGCCGCTCCACACCGCAg                                                                                                        >  2:1564702/1‑150 (MQ=255)
                                          gAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAg                                                                                                        >  2:1058478/1‑150 (MQ=255)
                                               gTTGACCCTGACGTCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCACCCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACtt                                                                                                   <  2:7754190/150‑1 (MQ=255)
                                               gTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACtt                                                                                                   >  2:10313687/1‑150 (MQ=255)
                                                 tGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCa                                                                                                 <  1:4550355/150‑1 (MQ=255)
                                                  gACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCAt                                                                                                >  2:8475544/1‑150 (MQ=255)
                                                    cccTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATcc                                                                                              >  2:6007477/1‑150 (MQ=255)
                                                       tGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAgc                                                                                           >  2:4063275/1‑150 (MQ=255)
                                                       tGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAgc                                                                                           <  1:5841323/150‑1 (MQ=255)
                                                             cAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGa                                                                                     >  2:4556348/1‑150 (MQ=255)
                                                             cAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGa                                                                                     <  2:5376589/150‑1 (MQ=255)
                                                             cAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGa                                                                                     <  1:3170460/150‑1 (MQ=255)
                                                              aGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAt                                                                                    >  2:2670889/1‑150 (MQ=255)
                                                               gTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAtg                                                                                   >  1:3519880/1‑150 (MQ=255)
                                                                 tCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAATCGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAtgct                                                                                 >  2:3416223/1‑150 (MQ=255)
                                                                  cgcgCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAtgctg                                                                                <  2:9235311/150‑1 (MQ=255)
                                                                   gcgcCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAtgctgc                                                                               >  2:9014996/1‑150 (MQ=255)
                                                                   gcgcCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGAtgctgc                                                                               <  1:9428663/150‑1 (MQ=255)
                                                                    cgcCCTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCg                                                                              >  2:10212850/1‑150 (MQ=255)
                                                                      cccTGCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGc                                                                            >  2:9129949/1‑150 (MQ=255)
                                                                        cTGCACTACGAGGATGTTGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCAGACCGACCGGCTGGTCCGTGTCGGCAAACTCGTCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCAt                                                                          >  2:6951898/1‑150 (MQ=255)
                                                                          gCAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGc                                                                        >  1:2129619/1‑150 (MQ=255)
                                                                           cAGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGcc                                                                       <  1:5575375/150‑1 (MQ=255)
                                                                            aGGACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGccc                                                                      >  1:10750095/1‑150 (MQ=255)
                                                                             ggACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCg                                                                     >  2:6290562/1‑150 (MQ=255)
                                                                             ggACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCg                                                                     >  2:5127401/1‑150 (MQ=255)
                                                                              gACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGc                                                                    >  2:10010469/1‑150 (MQ=255)
                                                                              gACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGc                                                                    >  2:4343379/1‑150 (MQ=255)
                                                                              gACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGc                                                                    <  1:4930900/150‑1 (MQ=255)
                                                                              gACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGc                                                                    >  2:875898/1‑150 (MQ=255)
                                                                              gACGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGc                                                                    >  1:1663959/1‑150 (MQ=255)
                                                                               aCGCGGATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGcc                                                                   >  2:5281996/1‑150 (MQ=255)
                                                                                   ggATGTGGTGATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAggg                                                               >  2:385213/1‑150 (MQ=255)
                                                                                       gtggtgATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGc                                                           >  2:7181263/1‑150 (MQ=255)
                                                                                         ggtgATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAg                                                         <  1:6563842/150‑1 (MQ=255)
                                                                                         ggtgATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAg                                                         >  2:9872960/1‑150 (MQ=255)
                                                                                          gtgATGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGc                                                        <  1:8398303/150‑1 (MQ=255)
                                                                                              tGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCAGCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCgtgg                                                    >  1:8697320/1‑150 (MQ=255)
                                                                                              tGGTCGACGATCTGGTCAACCCGAGCATACTGGAGCACGGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCgtgg                                                    <  2:10406488/150‑1 (MQ=255)
                                                                                                gTCGACGATCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCgtggtg                                                  <  2:3576817/150‑1 (MQ=255)
                                                                                                   gacgaTCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTgcgc                                               >  2:314595/1‑150 (MQ=255)
                                                                                                    acgaTCTGGTCAACCCGAGCATACTGGAGCACTGTCTGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGcc                                              >  1:7429650/1‑150 (MQ=255)
                                                                                                    acgaTCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGcc                                              >  2:589075/1‑150 (MQ=255)
                                                                                                    acgaTCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGcc                                              >  2:9594886/1‑150 (MQ=255)
                                                                                                     cgaTCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCt                                             >  1:2978571/1‑150 (MQ=255)
                                                                                                      gaTCTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTg                                            >  1:9312846/1‑150 (MQ=255)
                                                                                                       atctGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCACACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGa                                           >  1:1420564/4‑150 (MQ=255)
                                                                                                        tctGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGaa                                          <  1:1476328/148‑1 (MQ=255)
                                                                                                        tctGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGaa                                          >  1:8051826/3‑150 (MQ=255)
                                                                                                          tGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAg                                        >  1:51824/1‑150 (MQ=255)
                                                                                                          tGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAg                                        >  1:8867876/1‑150 (MQ=255)
                                                                                                           ggTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAgg                                       <  1:1766301/150‑1 (MQ=255)
                                                                                                           ggTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAgg                                       <  2:4941844/150‑1 (MQ=255)
                                                                                                           ggTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAgg                                       >  2:11077939/1‑150 (MQ=255)
                                                                                                              cAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAggcgg                                    <  1:9632844/150‑1 (MQ=255)
                                                                                                              cAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAggcgg                                    <  1:6482845/150‑1 (MQ=255)
                                                                                                               aaCCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAggcggc                                   <  2:813238/150‑1 (MQ=255)
                                                                                                                aCCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGgcggcg                                  >  2:8563517/1‑150 (MQ=255)
                                                                                                                  ccGAGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGAt                                <  2:2010401/150‑1 (MQ=255)
                                                                                                                     aGCATACTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCa                             >  1:8320717/1‑150 (MQ=255)
                                                                                                                           cTGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATc                       <  2:4764036/150‑1 (MQ=255)
                                                                                                                            tGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCt                      >  1:6211985/1‑150 (MQ=255)
                                                                                                                            tGGAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCt                      >  2:2266166/1‑150 (MQ=255)
                                                                                                                              gAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTc                    <  2:8819950/150‑1 (MQ=255)
                                                                                                                              gAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGGGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTc                    >  2:3244194/1‑150 (MQ=255)
                                                                                                                              gAGCACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACATCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTc                    >  1:6735032/1‑150 (MQ=255)
                                                                                                                                 cACTGTCCGTCCGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGc                 <  1:3897902/150‑1 (MQ=255)
                                                                                                                                     gtccgtccGCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCcgcg             >  1:8127444/1‑150 (MQ=255)
                                                                                                                                        cgtccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCg          <  2:9392416/150‑1 (MQ=255)
                                                                                                                                         gtccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCgg         <  1:5402957/150‑1 (MQ=255)
                                                                                                                                         gtccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCgg         >  1:634187/1‑150 (MQ=255)
                                                                                                                                           ccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCg       <  1:5342874/150‑1 (MQ=255)
                                                                                                                                           ccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCg       <  1:602822/150‑1 (MQ=255)
                                                                                                                                           ccgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCg       >  2:6763667/1‑150 (MQ=255)
                                                                                                                                            cgCCCGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCga      <  1:7952103/150‑1 (MQ=255)
                                                                                                                                              cccGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCgaag    >  1:4350445/1‑150 (MQ=255)
                                                                                                                                               ccGGCTGGTCCGGGTCGGCCAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGAGCCATGCATCTTCGGCCGCGCCGGCgaaga   >  1:7189459/1‑150 (MQ=255)
                                                                                                                                               ccGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCgaaga   >  2:7370117/1‑150 (MQ=255)
                                                                                                                                                cGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCgaagaa  >  2:570185/1‑150 (MQ=255)
                                                                                                                                                cGGCTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCgaagaa  <  1:10425334/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCGGTAAGGTCTGGCTGGTGGGTGCGGGTCCCGGCGATCCGGAACTGTTGACCCTGAAGGCAGTTCGCGCCCTGCAGGACGCGGATGTGGTGATGGTCGACGATTTGGTCAACCCGAGCATACTGGAGCACTGTCCGTCCGCCCGGGTGGTCCGGGTCGGCAAACGCGGCGGCTGCCGCTCCACACCGCAGGACTTCATCCAGCGCCTGATGCTGCGGCATGCCCGCCAGGGACGCAGCGTGGTGCGCCTGAAAGGCGGCGATCCATGCATCTTCGGCCGCGCCGGCGAAGAA  >  NC_008463/3711644‑3711937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑