Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,769,180 C→G A44A (GCG→GCC)  pscL ← SctL family type III secretion system stator protein PscL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,769,1800CG100.0% 1840.1 / NA 479A44A (GCG→GCC) pscLSctL family type III secretion system stator protein PscL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (250/229);  total (250/229)

Only 100 of 494 total aligned reads displayed.

GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326
                                                                                                                                                   |                                                                                                                                                  
gCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggc                                                                                                                                                  <  1:10891592/150‑1 (MQ=255)
   aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcggc                                                                                                                                               >  1:9980224/1‑150 (MQ=255)
   aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcggc                                                                                                                                               >  1:6056482/1‑150 (MQ=255)
   aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGAGTTCggcggc                                                                                                                                               >  2:10298023/1‑150 (MQ=255)
   aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGGTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcggc                                                                                                                                               <  1:3791073/150‑1 (MQ=255)
     cGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCat                                                                                                                                             <  2:9707338/150‑2 (MQ=255)
     cGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCAc                                                                                                                                             <  2:1915299/150‑1 (MQ=255)
      gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACg                                                                                                                                            >  2:2377876/1‑150 (MQ=255)
      gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACg                                                                                                                                            >  1:8244356/1‑150 (MQ=255)
      gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACg                                                                                                                                            <  1:5593662/150‑1 (MQ=255)
       aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                           >  2:4999753/1‑150 (MQ=255)
       aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                           >  1:7902335/1‑150 (MQ=255)
       aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCGTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATGTCGGCGGCACGc                                                                                                                                           >  1:3202718/1‑150 (MQ=255)
         cTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCCGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                         <  2:8099839/150‑1 (MQ=255)
         cTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                         <  1:4511959/150‑1 (MQ=255)
              ggTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTg                                                                                                                                    >  1:9096551/1‑150 (MQ=255)
               gtagtaGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGc                                                                                                                                   <  2:8901489/150‑1 (MQ=255)
                tagtagCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGcc                                                                                                                                  >  1:2714911/1‑150 (MQ=255)
                       gATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTcca                                                                                                                           >  2:8495407/1‑150 (MQ=255)
                          tGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCGCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTccacca                                                                                                                        <  1:6542740/150‑1 (MQ=255)
                           gCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAg                                                                                                                       <  2:7033666/150‑1 (MQ=255)
                                  aGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTg                                                                                                                >  2:2516770/1‑150 (MQ=255)
                                   gTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTgg                                                                                                               <  2:3208285/150‑1 (MQ=255)
                                      gCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcgc                                                                                                            <  1:6231493/150‑1 (MQ=255)
                                      gCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGgcgc                                                                                                            >  2:2225291/1‑150 (MQ=255)
                                        gTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcgcgg                                                                                                          >  1:6369412/1‑148 (MQ=255)
                                        gTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTg                                                                                                          >  2:1068242/1‑150 (MQ=255)
                                           tCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAgg                                                                                                       <  1:413658/150‑1 (MQ=255)
                                            ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCCCCAGGCGGTTGGCGCTGAGGt                                                                                                      >  1:10434482/1‑150 (MQ=255)
                                             cTGGATCTGCCCGGCCTGGCGCAGACGCGCCTCCTCCACTCCGGCCTCCCAGCCCAGTGGCTTCTGCTCCTGGTAAACCGCGTGGGCCTCTCGCTCGATTTCGGCGGCTCGCTCGGGTGCCGATTCCACCAGGCGGTTGGCGCTGAGGtt                                                                                                     <  2:9423304/150‑2 (MQ=255)
                                              tGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                    >  2:1595736/1‑150 (MQ=255)
                                                gATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTc                                                                                                  >  1:9190663/1‑150 (MQ=255)
                                                     gCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                             <  1:10775028/150‑1 (MQ=255)
                                                     gCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                             <  2:8298343/150‑1 (MQ=255)
                                                     gCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                             <  2:6582465/150‑1 (MQ=255)
                                                      cccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTa                                                                                            <  2:4575821/150‑1 (MQ=255)
                                                        cGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGt                                                                                          <  1:3515364/150‑1 (MQ=255)
                                                           ccTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcgc                                                                                       <  1:7450506/150‑1 (MQ=255)
                                                             tGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCgg                                                                                     >  2:9553701/1‑150 (MQ=255)
                                                              ggCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                    <  2:3925893/150‑1 (MQ=255)
                                                                  cAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgcg                                                                                >  2:11021777/1‑150 (MQ=255)
                                                                  cAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGGAGTCCTGGTAGTCGCGGgcgcg                                                                                >  1:9496332/1‑150 (MQ=255)
                                                                   aGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgcgc                                                                               <  1:5022548/150‑1 (MQ=255)
                                                                    gACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCa                                                                              >  1:10298750/1‑150 (MQ=255)
                                                                          cctcctCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCACCAggg                                                                        >  1:10050133/1‑150 (MQ=255)
                                                                          cctcctCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAggg                                                                        >  1:6688131/1‑150 (MQ=255)
                                                                          cctcctCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGGTGGGGCTGAGGTAGGCCTGGTAGTCGCGGGCGCGCAACAggg                                                                        >  1:1250825/1‑150 (MQ=255)
                                                                            tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                      >  1:1890824/1‑150 (MQ=255)
                                                                            tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                      <  1:305168/150‑1 (MQ=255)
                                                                              ctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTg                                                                    <  1:5855633/150‑1 (MQ=255)
                                                                              ctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGGGGGCCTCGCGCGCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCACCAGGGCCTg                                                                    >  2:10147256/1‑150 (MQ=255)
                                                                               tccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGc                                                                   >  1:2304153/1‑150 (MQ=255)
                                                                                ccAGTCCGGCCTCCCAGCCCCGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGcc                                                                  <  1:8638899/150‑1 (MQ=255)
                                                                                 cAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGccc                                                                 <  1:5248334/150‑1 (MQ=255)
                                                                                  aGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCg                                                                <  2:3660441/150‑1 (MQ=255)
                                                                                  aGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCg                                                                <  1:1294687/150‑1 (MQ=255)
                                                                                     ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                             <  1:7072176/150‑1 (MQ=255)
                                                                                       ggCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGc                                                           >  2:1451129/1‑150 (MQ=255)
                                                                                             ccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCg                                                     >  2:5158532/1‑150 (MQ=255)
                                                                                              cagcccagGCGCTTCTGCTCCTGGTAACCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGt                                                    <  1:10356696/150‑1 (MQ=255)
                                                                                              cagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGt                                                    <  1:812075/150‑1 (MQ=255)
                                                                                               agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTa                                                   >  1:2772266/1‑150 (MQ=255)
                                                                                                  ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                >  2:634406/1‑150 (MQ=255)
                                                                                                  ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                >  2:1762748/1‑150 (MQ=255)
                                                                                                    agGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGa                                              >  1:7557959/1‑150 (MQ=255)
                                                                                                     ggCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAc                                             >  1:213838/1‑150 (MQ=255)
                                                                                                     ggCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAc                                             <  2:7558247/150‑1 (MQ=255)
                                                                                                      gcgcTTCTGCTCCTGGTAAACCTCGTGGGCCACGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACt                                            <  1:9761511/150‑1 (MQ=255)
                                                                                                       cgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTg                                           >  2:5808188/1‑150 (MQ=255)
                                                                                                        gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGTTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTgg                                          <  2:2670968/150‑1 (MQ=255)
                                                                                                        gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTgg                                          <  2:7455072/150‑1 (MQ=255)
                                                                                                          ttCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCg                                        <  1:2574116/150‑1 (MQ=255)
                                                                                                          ttCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGGAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAGTGGCg                                        >  1:5654188/1‑150 (MQ=255)
                                                                                                            cTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTc                                      >  1:4772680/1‑150 (MQ=255)
                                                                                                             tGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCt                                     <  2:4330261/150‑1 (MQ=255)
                                                                                                              gCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                    <  2:5540212/150‑1 (MQ=255)
                                                                                                                   tGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTca                               <  2:5743635/150‑1 (MQ=255)
                                                                                                                    ggTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaa                              <  1:335788/150‑1 (MQ=255)
                                                                                                                    ggTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaa                              <  1:4429891/150‑1 (MQ=255)
                                                                                                                      tAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAAGCGACTGGCGGGGAGTTcaaca                            >  1:5100585/1‑150 (MQ=255)
                                                                                                                          ccTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                        <  2:3778556/150‑1 (MQ=255)
                                                                                                                          ccTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                        <  1:1757294/150‑1 (MQ=255)
                                                                                                                            tCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                      <  1:10158282/150‑1 (MQ=255)
                                                                                                                            tCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                      <  1:6931031/150‑1 (MQ=255)
                                                                                                                             cGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGaa                     <  2:2806107/150‑1 (MQ=255)
                                                                                                                              gTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAg                    <  2:5969838/150‑1 (MQ=255)
                                                                                                                               tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                   >  2:6873245/1‑150 (MQ=255)
                                                                                                                               tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                   >  2:9184409/1‑150 (MQ=255)
                                                                                                                                gggCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACACATGGACGCa                  >  2:7804967/1‑150 (MQ=255)
                                                                                                                                gggCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCa                  >  2:2742522/1‑150 (MQ=255)
                                                                                                                                    cTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAAGGGAAGCAGTCa              <  2:4174024/150‑1 (MQ=255)
                                                                                                                                     tCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAt             >  1:5079727/1‑150 (MQ=255)
                                                                                                                                        cgcTCGATTTCGGCGGCTCGCTCGCGTGCCGCGTCCACCAGGCGGGTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCCGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgc          >  2:3481921/1‑150 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        <  1:1359857/150‑1 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        >  1:7201196/1‑150 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        <  1:6032386/150‑1 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        >  1:1678233/1‑150 (MQ=255)
                                                                                                                                           tCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcg       >  1:8798801/1‑150 (MQ=255)
                                                                                                                                             gATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATcgctcgct     >  2:6228531/1‑150 (MQ=255)
                                                                                                                                              aTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt    >  2:71939/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑