Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,879,553 A→G F52L (TTC→CTC)  PA14_RS17695 ← LysE family translocator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,879,5530AG99.8% 1660.0 / NA 436F52L (TTC→CTC) PA14_RS17695LysE family translocator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (222/213);  minor base T (0/1);  total (222/214)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699
                                                                                                                                                   |                                                                                                                                                  
cGTCGCCCCGCCGCAGCCCGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGCTACAGCGCGCCGAGCGCCAGCAGGATGGCGAACAGCACAGGCGCACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGagaa                                                                                                                                                  <  2:7230640/150‑1 (MQ=255)
  tCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATc                                                                                                                                                >  1:8378860/1‑150 (MQ=255)
    gccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATccc                                                                                                                                              >  1:6270067/1‑150 (MQ=255)
     ccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCg                                                                                                                                             <  1:7431701/150‑1 (MQ=255)
     ccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCg                                                                                                                                             <  1:9912115/150‑1 (MQ=255)
      cgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGc                                                                                                                                            <  1:7540141/150‑1 (MQ=255)
      cgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGc                                                                                                                                            <  2:3730316/150‑1 (MQ=255)
       gcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCa                                                                                                                                           >  2:6877865/1‑150 (MQ=255)
          ccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGc                                                                                                                                        <  1:1074811/150‑1 (MQ=255)
          ccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGc                                                                                                                                        >  1:4619908/1‑150 (MQ=255)
              aGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGc                                                                                                                                    <  1:5125831/150‑1 (MQ=255)
              aGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGc                                                                                                                                    <  1:4283146/150‑1 (MQ=255)
               gCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcc                                                                                                                                   >  2:8310624/1‑150 (MQ=255)
               gCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcc                                                                                                                                   <  1:7913663/150‑1 (MQ=255)
                 cAGGCGCGCAGCGCGCCGACGCCGAGCCAGGGCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGGTGAGCAGCAGCGACTCGCCGGGCAGCTCCAGGGGGGTATGCAGGAGAATCGCGCTGGCCAGcccc                                                                                                                                 >  2:8623767/1‑150 (MQ=255)
                  aGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCa                                                                                                                                >  2:2370359/1‑150 (MQ=255)
                   ggCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAg                                                                                                                               >  2:10521487/1‑150 (MQ=255)
                   ggCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAg                                                                                                                               <  2:7997592/150‑1 (MQ=255)
                    gcgcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgc                                                                                                                              <  2:4063986/150‑1 (MQ=255)
                         cAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCt                                                                                                                         <  1:10611785/150‑1 (MQ=255)
                             gcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGcc                                                                                                                     >  2:10950823/1‑150 (MQ=255)
                              cgccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCa                                                                                                                    >  1:2744236/1‑150 (MQ=255)
                                 cgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGTCGAACAGCACAGGCGTACGGCTGAGCAGCAGAGACACGCCGGTCAGCTCCCGGGTGGTAAGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggc                                                                                                                 <  2:3977194/150‑1 (MQ=255)
                                 cgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggc                                                                                                                 <  1:3322136/150‑1 (MQ=255)
                                  gacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCCGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCCGGCCAGCCCCAGCGCGCTGGCCAggcc                                                                                                                >  2:455465/1‑150 (MQ=255)
                                  gacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggcc                                                                                                                >  2:1343552/1‑150 (MQ=255)
                                          gccaggccagGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccgcc                                                                                                        >  1:1017329/1‑150 (MQ=255)
                                          gccaggccagGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccgcc                                                                                                        >  1:4114692/1‑150 (MQ=255)
                                              ggccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGccc                                                                                                    >  2:3915905/1‑150 (MQ=255)
                                                  aggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCAGCGCAGCAGCGACACGCCGGCCAGCACCAGGGTGGGATGAAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTgg                                                                                                <  1:4834151/150‑1 (MQ=255)
                                                   ggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGt                                                                                               >  1:1482065/1‑150 (MQ=255)
                                                    gTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACCGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCTGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTg                                                                                              <  2:761010/150‑1 (MQ=255)
                                                    gTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTg                                                                                              >  2:6279414/1‑150 (MQ=255)
                                                    gTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTg                                                                                              >  1:9419734/1‑150 (MQ=255)
                                                         gcgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAggg                                                                                         <  1:8011010/150‑1 (MQ=255)
                                                             gcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGt                                                                                     >  1:8743947/1‑150 (MQ=255)
                                                              ccGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTg                                                                                    <  2:9497504/150‑1 (MQ=255)
                                                                gAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTgcg                                                                                  >  2:2975788/1‑150 (MQ=255)
                                                                     ccTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcacca                                                                             >  1:5617131/1‑150 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            >  2:3789183/1‑150 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCAGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            <  1:9119705/150‑1 (MQ=255)
                                                                       tGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCaccacc                                                                           >  2:5863828/1‑150 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      >  2:1145607/1‑150 (MQ=255)
                                                                             aTGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCGGGCCAGGCCGGCCCGCCGCCCCAGGTGCAGGGACGTGCGCGCCACCAGGGCg                                                                     >  2:400227/1‑150 (MQ=255)
                                                                                 cGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACAt                                                                 >  2:6105174/1‑150 (MQ=255)
                                                                                  gAACAGCACAGGCGTACGGCTGCTCAGCAGCGACACGCCGGTCAGCACCCGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGTCCATGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATc                                                                <  2:4222007/150‑1 (MQ=255)
                                                                                  gAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATc                                                                >  2:10873207/1‑150 (MQ=255)
                                                                                          caGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCCGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCgg                                                        >  1:452540/1‑150 (MQ=255)
                                                                                           aGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTCTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCggg                                                       >  2:1671927/1‑150 (MQ=255)
                                                                                           aGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCggg                                                       >  2:9290633/1‑150 (MQ=255)
                                                                                           aGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCggg                                                       <  1:10412602/150‑1 (MQ=255)
                                                                                            ggCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCgggg                                                      >  2:7464393/1‑150 (MQ=255)
                                                                                                tACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGa                                                  >  1:9638597/1‑150 (MQ=255)
                                                                                                  cGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGCCCGGGGCTGAcc                                                >  1:7194526/1‑150 (MQ=255)
                                                                                                   ggCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCa                                               <  1:1721689/150‑1 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              >  1:3830717/1‑150 (MQ=255)
                                                                                                      tGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAggg                                            >  1:815045/1‑150 (MQ=255)
                                                                                                      tGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAggg                                            <  1:5496726/150‑1 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  1:8642249/1‑150 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  1:4904212/1‑150 (MQ=255)
                                                                                                          cagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGAc                                        <  2:7749147/150‑1 (MQ=255)
                                                                                                           agcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACg                                       <  1:6981135/150‑1 (MQ=255)
                                                                                                              agcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCg                                    <  1:1372224/150‑1 (MQ=255)
                                                                                                                  acacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAaca                                <  2:8154524/150‑1 (MQ=255)
                                                                                                                   cacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAacac                               >  2:7346474/1‑150 (MQ=255)
                                                                                                                     cGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAccg                             <  1:3562519/150‑1 (MQ=255)
                                                                                                                      gCCGGTCAGCACCAGGGTGGTATGCAGGAGCAGCCCGCCGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAccgc                            <  2:9690192/150‑1 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         <  2:5084280/150‑1 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         >  1:5677410/1‑150 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACAGCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         >  2:10462230/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        >  1:924670/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        <  1:8882055/150‑1 (MQ=255)
                                                                                                                           tCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCa                       <  1:7744327/150‑1 (MQ=255)
                                                                                                                           tCAGCACCAGGGGGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACAGCGGGACCGGGGCTGACGAGGGCGACGGCGAACACCGCCGCCa                       >  2:5662715/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:3473893/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:3249354/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:2028779/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:8790965/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:4319405/1‑150 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  <  1:5802149/150‑1 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  >  2:5535184/1‑150 (MQ=255)
                                                                                                                                 ccAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCTCCACCTGGGCGACATCGGGACCGGGGCTGACCGGGGCGACGGCGAACACCGCCGCCCGGGTCa                 >  1:10231381/1‑150 (MQ=255)
                                                                                                                                  cAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAg                >  2:10118712/1‑150 (MQ=255)
                                                                                                                                   aGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGc               <  1:2435227/150‑1 (MQ=255)
                                                                                                                                    gggTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCa              <  1:5257347/150‑1 (MQ=255)
                                                                                                                                    gggTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGAGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCa              <  1:3118555/150‑1 (MQ=255)
                                                                                                                                      gtggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAag            <  2:107407/150‑1 (MQ=255)
                                                                                                                                       tggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAaga           <  1:11051099/150‑1 (MQ=255)
                                                                                                                                       tggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAaga           <  2:2148198/150‑1 (MQ=255)
                                                                                                                                        ggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagac          <  2:315527/150‑1 (MQ=255)
                                                                                                                                        ggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagac          >  1:10024263/1‑150 (MQ=255)
                                                                                                                                           aTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacaga       <  1:6517834/150‑1 (MQ=255)
                                                                                                                                            tGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacagac      >  2:6430286/1‑150 (MQ=255)
                                                                                                                                             gCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAgacagaca     >  1:6766948/1‑150 (MQ=255)
                                                                                                                                              cAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACaa    >  2:10294701/1‑150 (MQ=255)
                                                                                                                                              cAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACaa    <  1:1992366/150‑1 (MQ=255)
                                                                                                                                               aGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAt   <  1:9876622/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  2:263352/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  1:9497868/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCGGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  >  2:6629205/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑