Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,760,7430AG100.0% 1436.3 / NA 375L73P (CTT→CCT) PA14_RS21850HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (191/184);  total (191/184)

Only 100 of 391 total aligned reads displayed.

ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889
                                                                                                                                                   |                                                                                                                                                  
aTCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAggg                                                                                                                                                  >  2:3823661/1‑150 (MQ=255)
  ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTc                                                                                                                                                >  2:8276046/1‑150 (MQ=255)
    cccGGACACCACTACGCCTGTAGTTGCTGGGCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGTTCACCTTTCGAGCTTTCAGGGTCAg                                                                                                                                              <  2:7036939/150‑1 (MQ=255)
    cccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAg                                                                                                                                              <  1:8485809/150‑1 (MQ=255)
    cccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAg                                                                                                                                              <  1:3019080/150‑1 (MQ=255)
     ccccacacCACTACGCCACGACTTGCTGGCCCTCGACGGCGGACTGGGCACACGGCAGCGAGACCCTTCCTGTGCCAGGACTGCCTCCTCAAATGGCTGAAAACTCTCGCTCAACGCGAAGAGATCACCGCTCGAGCTATCAGCGTCAgg                                                                                                                                             <  2:2564398/146‑1 (MQ=255)
     ccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAgg                                                                                                                                             >  2:107890/1‑150 (MQ=255)
      cGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTtcagggtcaggg                                                                                                                                            <  2:4545498/150‑1 (MQ=255)
        gACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTa                                                                                                                                          >  1:8860293/1‑150 (MQ=255)
         acacCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         <  2:6849453/150‑1 (MQ=255)
         acacCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         <  1:2410581/150‑1 (MQ=255)
          caccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaaa                                                                                                                                        <  2:6450265/150‑1 (MQ=255)
            ccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAcc                                                                                                                                      >  1:3133055/1‑150 (MQ=255)
             cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCt                                                                                                                                     <  1:9073980/150‑1 (MQ=255)
               cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   <  2:3466384/150‑1 (MQ=255)
               cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   >  1:1854127/1‑150 (MQ=255)
                tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCACATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGc                                                                                                                                  <  1:8952171/150‑1 (MQ=255)
                 aCGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGcc                                                                                                                                 <  1:5026607/150‑1 (MQ=255)
                  cGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCg                                                                                                                                >  1:4428361/1‑150 (MQ=255)
                   gCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGa                                                                                                                               <  1:8314281/150‑1 (MQ=255)
                     cAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATa                                                                                                                             <  1:6314615/150‑1 (MQ=255)
                       gtagtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcg                                                                                                                           >  1:6412207/1‑150 (MQ=255)
                         agtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcgcg                                                                                                                         <  1:9150378/150‑1 (MQ=255)
                         agtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcgcg                                                                                                                         >  2:7169118/1‑150 (MQ=255)
                          gtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACgcgc                                                                                                                        <  1:4166769/150‑1 (MQ=255)
                           ttGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCa                                                                                                                       <  2:6714935/150‑1 (MQ=255)
                            tGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCaa                                                                                                                      <  1:5743054/150‑1 (MQ=255)
                                    ttCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAg                                                                                                              >  2:1211277/1‑150 (MQ=255)
                                     tCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGt                                                                                                             <  1:4235726/150‑1 (MQ=255)
                                        aTGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAAt                                                                                                          <  2:9594933/150‑1 (MQ=255)
                                         tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCCAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTCATg                                                                                                         >  2:5944652/1‑150 (MQ=255)
                                         tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATg                                                                                                         >  2:197384/1‑150 (MQ=255)
                                          ggCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                        <  2:4693366/150‑1 (MQ=255)
                                          ggCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                        <  1:2699042/150‑1 (MQ=255)
                                           gCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCg                                                                                                       >  1:6018696/1‑150 (MQ=255)
                                             ggAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGc                                                                                                     <  2:2084399/150‑1 (MQ=255)
                                               aaTGGGCACTAGGAAGCTAATCCCTACATTTCCCAGGACTGCCTGCACAAATGGCTGAAAACACACGAAAAACTCGAAGAGATCACCTTTCGAGCATTCAGGGTCAGGGTAAACCATGCCGATAAGCGCAATACCAAAGTAATGCGGCTg                                                                                                   >  2:7688477/1‑150 (MQ=255)
                                                aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACAAAAGTAATGCGGCTGa                                                                                                  >  2:5766538/1‑150 (MQ=255)
                                                 tGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAg                                                                                                 <  2:10969354/150‑1 (MQ=255)
                                                  gggCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGc                                                                                                >  1:10430202/1‑150 (MQ=255)
                                                       ctAGGTAGCGATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGa                                                                                           <  2:8722901/150‑1 (MQ=255)
                                                         aGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAgg                                                                                         >  2:7587539/1‑150 (MQ=255)
                                                              gCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTg                                                                                    <  1:1357791/150‑1 (MQ=255)
                                                                tATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGAt                                                                                  <  1:519038/150‑1 (MQ=255)
                                                                  ttCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCa                                                                                >  2:6945867/1‑150 (MQ=255)
                                                                   tCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAg                                                                               >  1:7925338/1‑150 (MQ=255)
                                                                     ccTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCa                                                                             >  2:836559/1‑150 (MQ=255)
                                                                      cttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaa                                                                            >  1:5886891/1‑150 (MQ=255)
                                                                      cttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGAGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGATGATCAGCaa                                                                            >  1:10994244/1‑150 (MQ=255)
                                                                       ttcttTTGCCCGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaaa                                                                           >  2:7485541/1‑150 (MQ=255)
                                                                        tcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAg                                                                          <  2:3682565/150‑1 (MQ=255)
                                                                             tGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     <  1:5540614/150‑1 (MQ=255)
                                                                              gCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCTAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGACGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTa                                                                    >  2:156689/1‑150 (MQ=255)
                                                                              gCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTa                                                                    <  2:4953120/150‑1 (MQ=255)
                                                                               ccAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTAt                                                                   >  1:9926625/1‑150 (MQ=255)
                                                                               ccAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTAt                                                                   >  2:1414634/1‑150 (MQ=255)
                                                                                cAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATc                                                                  >  2:2023396/1‑150 (MQ=255)
                                                                                cAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATc                                                                  <  2:4376045/150‑1 (MQ=255)
                                                                                    aCTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTc                                                              <  1:7024192/150‑1 (MQ=255)
                                                                                     ctgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCa                                                             <  1:10627502/150‑1 (MQ=255)
                                                                                       gcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGc                                                           >  1:8188732/1‑150 (MQ=255)
                                                                                                 aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAGGGACTTATCTGTCAGCTTGCCCTTgt                                                 >  2:2181022/1‑150 (MQ=255)
                                                                                                 aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgt                                                 <  2:1758804/150‑1 (MQ=255)
                                                                                                 aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgt                                                 <  2:6491532/150‑1 (MQ=255)
                                                                                                  tGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgta                                                >  2:10319631/1‑150 (MQ=255)
                                                                                                  tGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgta                                                <  1:9340381/150‑1 (MQ=255)
                                                                                                   ggCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtag                                               <  2:5349979/150‑1 (MQ=255)
                                                                                                   ggCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtag                                               <  1:1419546/150‑1 (MQ=255)
                                                                                                    gCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtagt                                              <  1:10577377/150‑1 (MQ=255)
                                                                                                    gCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtagt                                              >  2:2288254/1‑150 (MQ=255)
                                                                                                       gAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTaaa                                           >  2:4139946/1‑150 (MQ=255)
                                                                                                        aaaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAac                                          >  1:8703368/1‑150 (MQ=255)
                                                                                                         aaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacc                                         >  1:7227377/1‑150 (MQ=255)
                                                                                                            ctctCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccacc                                      <  2:5502954/150‑1 (MQ=255)
                                                                                                            ctctCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccacc                                      <  1:4617334/150‑1 (MQ=255)
                                                                                                             tctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCt                                     <  1:2097660/150‑1 (MQ=255)
                                                                                                              ctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTg                                    <  1:8897841/150‑1 (MQ=255)
                                                                                                                cGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                  <  2:564836/150‑1 (MQ=255)
                                                                                                                 gATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAc                                 <  2:7922563/150‑1 (MQ=255)
                                                                                                                  aTCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcc                                <  2:279887/150‑1 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              <  2:4797980/150‑1 (MQ=255)
                                                                                                                       cgcgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTgg                           <  1:9751633/150‑1 (MQ=255)
                                                                                                                          gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCtt                        >  1:7530175/1‑150 (MQ=255)
                                                                                                                          gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCtt                        >  1:9472193/1‑150 (MQ=255)
                                                                                                                           aaGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       <  1:4063610/150‑1 (MQ=255)
                                                                                                                                aTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGAt                  <  2:8916720/150‑1 (MQ=255)
                                                                                                                                aTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGAt                  <  2:9767949/150‑1 (MQ=255)
                                                                                                                                   aCCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgc               <  2:8495250/150‑1 (MQ=255)
                                                                                                                                     cTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgcgc             <  1:3923482/150‑1 (MQ=255)
                                                                                                                                      tttCGAGCTTTCAGGGTCTGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCt            <  2:10062702/150‑1 (MQ=255)
                                                                                                                                       ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCTGCAAAGGACTTATCTGTCAGCTTGCCCGTGTCGTAAACCACCTGCACCCCGGGCTGAAGGATGGCGCTg           >  2:6337736/1‑150 (MQ=255)
                                                                                                                                        tCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACAGAGGCGCTGATCAGCAAAGGACCTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt          <  2:9213429/150‑1 (MQ=255)
                                                                                                                                          gAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTAt        >  1:7716921/1‑150 (MQ=255)
                                                                                                                                          gAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTAt        >  1:6664367/1‑150 (MQ=255)
                                                                                                                                          gAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCGGTAt        <  2:10579079/150‑1 (MQ=255)
                                                                                                                                           aGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATc       >  1:7614155/1‑150 (MQ=255)
                                                                                                                                            gCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCg      >  2:3018851/1‑150 (MQ=255)
                                                                                                                                              tttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGcc    >  2:308017/1‑150 (MQ=255)
                                                                                                                                              tttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGcc    >  1:4849503/1‑150 (MQ=255)
                                                                                                                                               ttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCt   >  1:10017354/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑