Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,530,315 A→G K229K (AAA→AAG)  PA14_RS25330 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,530,3150AG100.0% 1457.8 / NA 374K229K (AAA→AAG) PA14_RS25330iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (200/174);  total (200/174)

Only 100 of 404 total aligned reads displayed.

CAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGA  >  NC_008463/5530168‑5530461
                                                                                                                                                   |                                                                                                                                                  
cAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                  >  2:246721/1‑150 (MQ=255)
  aGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagg                                                                                                                                                <  1:2622913/150‑1 (MQ=255)
     gCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACCACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaa                                                                                                                                             <  1:9995355/150‑1 (MQ=255)
         ggaggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAggg                                                                                                                                         >  2:5088554/1‑150 (MQ=255)
            ggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAATGGGCGa                                                                                                                                      >  1:3676374/1‑150 (MQ=255)
                   gAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAc                                                                                                                               <  1:5967613/150‑1 (MQ=255)
                   gAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAc                                                                                                                               >  1:7499022/1‑150 (MQ=255)
                    aGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACt                                                                                                                              >  2:5129918/1‑150 (MQ=255)
                     ggATTGGCTGACCTGCCTGAAGGCCTTCTGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTc                                                                                                                             <  2:7902131/150‑1 (MQ=255)
                     ggATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTc                                                                                                                             >  1:3396185/1‑150 (MQ=255)
                      gATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcg                                                                                                                            <  1:7128905/150‑1 (MQ=255)
                       aTTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcgc                                                                                                                           >  1:1931580/1‑150 (MQ=255)
                        ttGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcgcg                                                                                                                          <  1:10758/150‑1 (MQ=255)
                        ttGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcgcg                                                                                                                          >  1:8980700/1‑150 (MQ=255)
                          ggCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGcc                                                                                                                        <  1:6743704/150‑1 (MQ=255)
                           gCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                       >  1:8095433/1‑150 (MQ=255)
                            cTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTg                                                                                                                      >  1:6091500/1‑150 (MQ=255)
                            cTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTg                                                                                                                      >  2:7415268/1‑150 (MQ=255)
                             tGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCTCCATTAAAGCCGTGTAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGTGAGAGTTGAACTCGCGCCTGc                                                                                                                     >  1:4984854/1‑150 (MQ=255)
                              gACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCa                                                                                                                    >  1:9656992/1‑150 (MQ=255)
                                ccGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGACGGAAAAGGGCGAGTTGAACTCGCGCCTGCact                                                                                                                  >  2:7592950/1‑150 (MQ=255)
                                  ggCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactac                                                                                                                <  2:9619680/150‑1 (MQ=255)
                                    ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACtt                                                                                                              <  2:10105571/150‑1 (MQ=255)
                                      tGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCg                                                                                                            >  1:10008482/1‑150 (MQ=255)
                                       gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGACGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCgg                                                                                                           <  1:784234/150‑1 (MQ=255)
                                          ggCCTTCGGCTCGATCTACACCACCAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACGCCTGGTACACCCTGAAGAAGGAAAAGCGCGAGTTGAACTCGCGCCTGCACTACTTCGGCaa                                                                                                        <  1:448171/150‑1 (MQ=255)
                                            ccTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAAcc                                                                                                      >  2:4936558/1‑150 (MQ=255)
                                              ttCTGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                    <  2:8296271/150‑1 (MQ=255)
                                               tCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAgg                                                                                                   >  1:10629751/1‑150 (MQ=255)
                                                         cTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgc                                                                                         <  1:5593232/150‑1 (MQ=255)
                                                          tACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgcg                                                                                        <  1:4559344/150‑1 (MQ=255)
                                                           acacCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgcgc                                                                                       >  1:10197484/1‑150 (MQ=255)
                                                              ccAACACCGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTgg                                                                                    <  1:1718079/150‑1 (MQ=255)
                                                              ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTgg                                                                                    >  2:8432761/1‑150 (MQ=255)
                                                                aacaccGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTc                                                                                  <  1:1487297/150‑1 (MQ=255)
                                                                   aacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCTACCAGGACCCGGGCGCGCTGGTCAcc                                                                               >  1:6460305/1‑150 (MQ=255)
                                                                    acGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCg                                                                              >  1:2278482/1‑150 (MQ=255)
                                                                    acGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCg                                                                              >  2:3114192/1‑150 (MQ=255)
                                                                       tCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtct                                                                           <  1:6695964/150‑1 (MQ=255)
                                                                        cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtctc                                                                          <  1:10471318/150‑1 (MQ=255)
                                                                        cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtctc                                                                          >  2:10480737/1‑150 (MQ=255)
                                                                         aCCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTcc                                                                         <  1:4965852/150‑1 (MQ=255)
                                                                          ccGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCg                                                                        >  1:8292881/1‑150 (MQ=255)
                                                                          ccGCCACGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACCACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCg                                                                        <  1:2117814/150‑1 (MQ=255)
                                                                          cagCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTCCACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCg                                                                        >  2:881103/3‑150 (MQ=255)
                                                                              cATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcgc                                                                    <  1:8751088/150‑1 (MQ=255)
                                                                                tGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgccg                                                                  >  2:6794508/1‑150 (MQ=255)
                                                                                 gAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACACCTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGCACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgccgc                                                                 <  2:10697416/150‑1 (MQ=255)
                                                                                   aaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                               >  1:4284664/1‑150 (MQ=255)
                                                                                    aGCCGAGTAGAAAGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGt                                                              >  2:10345589/1‑150 (MQ=255)
                                                                                     gCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTg                                                             <  2:1889263/150‑1 (MQ=255)
                                                                                      ccGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGc                                                            <  2:5485157/150‑1 (MQ=255)
                                                                                         tGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCa                                                         <  1:2054518/150‑1 (MQ=255)
                                                                                         tGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCa                                                         <  1:2815671/150‑1 (MQ=255)
                                                                                          ggAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCaa                                                        <  2:4580976/150‑1 (MQ=255)
                                                                                              aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                    <  1:1326892/150‑1 (MQ=255)
                                                                                               aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                   <  2:1732863/150‑1 (MQ=255)
                                                                                               aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                   <  2:6435044/150‑1 (MQ=255)
                                                                                               aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                   >  2:6724158/1‑150 (MQ=255)
                                                                                                cGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCag                                                  >  2:2903650/1‑150 (MQ=255)
                                                                                                 ggCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCACCCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagc                                                 >  1:10590739/1‑150 (MQ=255)
                                                                                                   cGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaa                                               <  1:8485881/150‑1 (MQ=255)
                                                                                                   cGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACGTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaa                                               <  2:9805166/150‑1 (MQ=255)
                                                                                                     aGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCCACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaagc                                             >  1:3204161/1‑150 (MQ=255)
                                                                                                      ggTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGAGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcacgca                                            >  1:10990716/1‑150 (MQ=255)
                                                                                                        tCGACATGGCGCTGATCAACAACTACTCCTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcc                                          <  1:5452641/150‑1 (MQ=255)
                                                                                                          gACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcccc                                        >  1:9726389/1‑150 (MQ=255)
                                                                                                            cATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAg                                      >  2:1374110/1‑150 (MQ=255)
                                                                                                             aTGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAgg                                     >  2:2769932/1‑150 (MQ=255)
                                                                                                                gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGaa                                  <  2:3212282/150‑1 (MQ=255)
                                                                                                                 cgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                 <  1:9150110/150‑1 (MQ=255)
                                                                                                                     gATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                             >  1:3867123/1‑150 (MQ=255)
                                                                                                                      aTCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcag                            <  2:11008136/150‑1 (MQ=255)
                                                                                                                       tCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagc                           <  2:8048401/150‑1 (MQ=255)
                                                                                                                        caacaaCTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagca                          >  1:1666978/1‑150 (MQ=255)
                                                                                                                         aacaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagcag                         >  2:2160207/1‑150 (MQ=255)
                                                                                                                          acaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTAAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGt                        >  2:3560711/1‑150 (MQ=255)
                                                                                                                           caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                       <  1:7805573/150‑1 (MQ=255)
                                                                                                                           caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                       <  1:6299198/150‑1 (MQ=255)
                                                                                                                            aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtc                      <  2:9455683/150‑1 (MQ=255)
                                                                                                                             actactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                     >  1:3162519/1‑150 (MQ=255)
                                                                                                                             actactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                     >  1:5739187/1‑150 (MQ=255)
                                                                                                                              ctactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgt                    <  2:577450/150‑1 (MQ=255)
                                                                                                                               tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtc                   <  2:9113700/150‑1 (MQ=255)
                                                                                                                               tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtc                   <  2:5627777/150‑1 (MQ=255)
                                                                                                                                actactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                  >  2:4096311/1‑150 (MQ=255)
                                                                                                                                actactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                  >  2:3866053/1‑150 (MQ=255)
                                                                                                                                  tactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                >  2:8717616/1‑150 (MQ=255)
                                                                                                                                    ctGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt              >  2:637330/1‑150 (MQ=255)
                                                                                                                                      ggTACACCCTGAAGAAGGAAAAGGGCGTGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCACGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCa            >  1:3618528/1‑150 (MQ=255)
                                                                                                                                      ggTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCa            >  1:8643118/1‑150 (MQ=255)
                                                                                                                                         acacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGc         <  1:8943886/150‑1 (MQ=255)
                                                                                                                                           acCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc       <  2:9031660/150‑1 (MQ=255)
                                                                                                                                            cccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCa      >  1:9584145/1‑150 (MQ=255)
                                                                                                                                            cccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCa      >  1:7947475/1‑150 (MQ=255)
                                                                                                                                             ccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAg     <  2:7067632/150‑1 (MQ=255)
                                                                                                                                              cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGc    >  1:3056319/1‑150 (MQ=255)
                                                                                                                                              cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGc    >  1:1112962/1‑150 (MQ=255)
                                                                                                                                                gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGa  >  2:3398500/1‑150 (MQ=255)
                                                                                                                                                gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGa  <  1:4769405/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGA  >  NC_008463/5530168‑5530461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑