Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463927,9170TC99.7% 2195.3 / NA 578I56T (ATC→ACC) PA14_RS04340PAS domain S‑box protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (257/319);  minor base A (0/2);  total (257/321)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 585 total aligned reads displayed.

AGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCT  >  NC_008463/927770‑928063
                                                                                                                                                   |                                                                                                                                                  
aGTTCAGGCTTAGCCGCTGGGCGGGCCCGACACTGGCTCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCCGATGGCGCTGGAACGCGAGGCTGAACTGCTGGCCGAGGCGGTGAccc                                                                                                                                                  >  2:2971929/1‑150 (MQ=255)
aGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGAccc                                                                                                                                                  <  1:7817726/150‑1 (MQ=255)
 gTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccg                                                                                                                                                 <  1:461283/150‑1 (MQ=255)
  ttCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgc                                                                                                                                                >  1:10067294/1‑150 (MQ=255)
     aGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACCGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCcgccgc                                                                                                                                             >  2:1277234/1‑150 (MQ=255)
      ggCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                            >  1:9567551/1‑150 (MQ=255)
        cTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                          <  1:2076808/150‑1 (MQ=255)
        cTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                          >  1:2594162/1‑150 (MQ=255)
          gAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGa                                                                                                                                        >  2:1422668/1‑150 (MQ=255)
           aGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGaa                                                                                                                                       >  1:6602581/1‑150 (MQ=255)
               gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActct                                                                                                                                   >  2:9739594/1‑150 (MQ=255)
               gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActct                                                                                                                                   >  2:2463148/1‑150 (MQ=255)
                cTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActctc                                                                                                                                  >  2:4135547/1‑149 (MQ=255)
                cTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTa                                                                                                                                  >  1:5589283/1‑150 (MQ=255)
                cTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTa                                                                                                                                  <  1:541392/150‑1 (MQ=255)
                  gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTAcc                                                                                                                                >  1:8050153/1‑150 (MQ=255)
                   ggcgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                               <  2:8396467/150‑1 (MQ=255)
                     cgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGt                                                                                                                             <  2:4373562/150‑1 (MQ=255)
                     cgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGt                                                                                                                             >  1:7111201/1‑150 (MQ=255)
                        gcgcGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATg                                                                                                                          >  2:6611578/1‑150 (MQ=255)
                        gcgcGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATg                                                                                                                          <  1:7493415/150‑1 (MQ=255)
                           cgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCt                                                                                                                       <  1:5900858/150‑1 (MQ=255)
                            gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCtt                                                                                                                      >  1:9658060/1‑150 (MQ=255)
                            gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCtt                                                                                                                      >  2:10149183/1‑150 (MQ=255)
                              cacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGc                                                                                                                    >  1:1237302/1‑150 (MQ=255)
                                cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                  <  1:8557453/150‑1 (MQ=255)
                                cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                  >  2:10483913/1‑150 (MQ=255)
                                cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGATGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                  >  1:10914284/1‑150 (MQ=255)
                                 tGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTg                                                                                                                 <  2:2876234/150‑1 (MQ=255)
                                    cgcTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTgggg                                                                                                              <  2:10380992/150‑1 (MQ=255)
                                      cTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTg                                                                                                            <  1:3384059/150‑1 (MQ=255)
                                      cTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTg                                                                                                            >  2:5550755/1‑150 (MQ=255)
                                         ggACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcgc                                                                                                         <  2:686332/150‑1 (MQ=255)
                                          gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                        >  2:6207462/1‑150 (MQ=255)
                                          gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                        <  1:3080368/150‑1 (MQ=255)
                                           aCTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCgg                                                                                                       <  1:363240/150‑1 (MQ=255)
                                            cTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                      >  1:7927344/1‑150 (MQ=255)
                                            cTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                      <  1:9103431/150‑1 (MQ=255)
                                            cTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                      <  2:5009094/150‑1 (MQ=255)
                                             tGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTg                                                                                                     <  2:5974456/150‑1 (MQ=255)
                                                tGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCt                                                                                                  >  2:6723691/1‑150 (MQ=255)
                                                 gCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGACCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCtt                                                                                                 >  2:7728566/1‑150 (MQ=255)
                                                   tGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGc                                                                                               >  2:9315040/1‑150 (MQ=255)
                                                       cGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGAc                                                                                           <  2:4847990/150‑1 (MQ=255)
                                                        gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                          >  2:6525649/1‑150 (MQ=255)
                                                        gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                          >  2:10006132/1‑150 (MQ=255)
                                                        gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                          >  1:1510692/1‑150 (MQ=255)
                                                         ggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                         <  2:8120680/150‑1 (MQ=255)
                                                         ggCTCGGCGCCTGGGGGCTGGCGCTGGTCCACGAGCTACAGGCGCGGATGGCGCTGGTACGCGCGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCGACCAGGAGGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                         <  2:10625495/150‑1 (MQ=255)
                                                           cTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCg                                                                                       <  2:3364813/150‑1 (MQ=255)
                                                             gggCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCggg                                                                                     >  2:2215836/1‑150 (MQ=255)
                                                              ggCGCCTGGGGGCTGGCGCTGGTCCACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGc                                                                                    >  2:729058/1‑150 (MQ=255)
                                                              ggCGCCTGGGGGCTGGCGCTGGTCAACGAGCAAAAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGc                                                                                    >  2:8048888/1‑150 (MQ=255)
                                                               gcgcCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCg                                                                                   <  1:10556981/150‑1 (MQ=255)
                                                                cgcCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACTGCGGGCGa                                                                                  >  2:2076608/1‑150 (MQ=255)
                                                                  ccTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAgg                                                                                <  2:2662568/150‑1 (MQ=255)
                                                                  ccTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAgg                                                                                <  1:4902483/150‑1 (MQ=255)
                                                                     gggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcgc                                                                             >  2:6136544/1‑150 (MQ=255)
                                                                       gggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcac                                                                           <  2:3224864/150‑1 (MQ=255)
                                                                         gCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACAt                                                                         <  1:6878532/150‑1 (MQ=255)
                                                                             gcgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAc                                                                     <  2:8959432/150‑1 (MQ=255)
                                                                                cTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgc                                                                  <  2:11031183/150‑1 (MQ=255)
                                                                                  ggTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTATACCAGTATGGATTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGa                                                                <  1:3827017/150‑1 (MQ=255)
                                                                                    tCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGAGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGacc                                                              >  2:3855132/1‑148 (MQ=255)
                                                                                      aaCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTg                                                            >  2:5373549/1‑150 (MQ=255)
                                                                                       aCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGt                                                           >  1:8725050/1‑150 (MQ=255)
                                                                                          aGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                        <  1:9338226/150‑1 (MQ=255)
                                                                                          aGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTcc                                                        <  2:1603655/150‑1 (MQ=255)
                                                                                            cAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgc                                                      >  2:3644432/1‑150 (MQ=255)
                                                                                            cAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgc                                                      <  2:9224949/150‑1 (MQ=255)
                                                                                            cAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgc                                                      <  1:1756472/150‑1 (MQ=255)
                                                                                               cAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCcgccgc                                                   <  2:5620728/150‑1 (MQ=255)
                                                                                               cAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCcgccgc                                                   <  1:10809127/150‑1 (MQ=255)
                                                                                                 ggCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTa                                                 >  2:1914513/1‑150 (MQ=255)
                                                                                                       gagggCGCTGGAACGCGAGGCGGAACTGCGGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                           <  2:4721071/147‑1 (MQ=255)
                                                                                                       gATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCt                                           <  2:9542525/150‑1 (MQ=255)
                                                                                                        aTGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTg                                          <  1:4253379/150‑1 (MQ=255)
                                                                                                         tGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGa                                         >  1:8344950/1‑150 (MQ=255)
                                                                                                          ggCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAc                                        <  2:7517561/150‑1 (MQ=255)
                                                                                                               tGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgcg                                   >  2:587177/1‑150 (MQ=255)
                                                                                                                ggAACGCGAGGCGGAACTGCGGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGa                                  <  1:2781564/150‑1 (MQ=255)
                                                                                                                   aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                               <  1:10269194/150‑1 (MQ=255)
                                                                                                                   aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                               <  2:7409550/150‑1 (MQ=255)
                                                                                                                   aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                               <  2:7119056/150‑1 (MQ=255)
                                                                                                                   aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCGTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                               <  1:6122704/150‑1 (MQ=255)
                                                                                                                      cgAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGa                            <  1:4969202/150‑1 (MQ=255)
                                                                                                                        aGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                          <  2:6313699/150‑1 (MQ=255)
                                                                                                                         ggCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcg                         <  2:353052/150‑1 (MQ=255)
                                                                                                                           cGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgcg                       >  1:3929983/1‑150 (MQ=255)
                                                                                                                             gAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                     >  1:5700168/1‑150 (MQ=255)
                                                                                                                             gAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                     <  1:479529/150‑1 (MQ=255)
                                                                                                                             gAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAGGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                     <  2:8626364/150‑1 (MQ=255)
                                                                                                                                     ggCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg             >  2:10293008/1‑150 (MQ=255)
                                                                                                                                          aGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGccc        <  1:1381301/150‑1 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      <  1:6716715/150‑1 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  2:8729408/1‑150 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  2:5140576/1‑150 (MQ=255)
                                                                                                                                             cGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgcg     <  2:10882021/150‑1 (MQ=255)
                                                                                                                                              ggTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcgg    >  2:4500778/1‑150 (MQ=255)
                                                                                                                                               gTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc   <  2:7814119/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCT  >  NC_008463/927770‑928063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑