Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,356,5480GC99.4% 2042.9 / NA 534R69G (CGT→GGT) PA14_RS06375MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (200/330);  minor base A (2/1);  total (202/332)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 534 total aligned reads displayed.

TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694
                                                                                                                                                   |                                                                                                                                                  
 gCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGaa                                                                                                                                                 <  1:5244880/150‑1 (MQ=255)
     tCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                             <  2:7131907/150‑1 (MQ=255)
         aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGa                                                                                                                                         >  1:6771538/1‑150 (MQ=255)
         aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGa                                                                                                                                         <  1:5359142/150‑1 (MQ=255)
          gCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                        <  1:6587029/150‑1 (MQ=255)
          gCCGGGGAGGACGCCGATCAGGGTGGTGGAGACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                        <  2:7699292/150‑1 (MQ=255)
            cGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAgg                                                                                                                                      >  2:10343/1‑150 (MQ=255)
              gggAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgcg                                                                                                                                    >  2:8048849/1‑150 (MQ=255)
                    aCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAATGCGATACCGAAGGTGAGGAAGGCGCTGAGc                                                                                                                              <  2:9068195/150‑1 (MQ=255)
                     cGCCGATGAGGGTTGTGGATAACCCCATCATCAGCAGCGAGGCGTACAGGGTTGACTTGCGCCCGATGCGGTAGCCGAAGTTGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGTAAGGCGATACCGAAGGTGAGGAAGGAGCTGAGCa                                                                                                                             <  2:9761599/150‑1 (MQ=255)
                       ccGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATc                                                                                                                           >  1:5770487/1‑150 (MQ=255)
                         gATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTg                                                                                                                         >  1:6448014/1‑150 (MQ=255)
                          aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTGGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                        <  2:2414616/150‑1 (MQ=255)
                              gggTGGTGGATACCCCCATCAGCAGGATCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCgg                                                                                                                    <  2:4944519/150‑1 (MQ=255)
                              gggTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCgg                                                                                                                    >  2:3296880/1‑150 (MQ=255)
                              gggTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCgg                                                                                                                    >  2:7784189/1‑150 (MQ=255)
                              gggTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCgg                                                                                                                    <  2:3798540/150‑1 (MQ=255)
                               ggtggtGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGt                                                                                                                   <  1:3684691/150‑1 (MQ=255)
                                gtggtgGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTg                                                                                                                  >  2:1360805/1‑150 (MQ=255)
                                 tggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGc                                                                                                                 >  2:2900984/1‑150 (MQ=255)
                                 tggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGc                                                                                                                 >  2:8421540/1‑150 (MQ=255)
                                  ggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGcc                                                                                                                <  1:3167712/150‑1 (MQ=255)
                                  ggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGcc                                                                                                                <  2:9413516/150‑1 (MQ=255)
                                   gtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCg                                                                                                               <  1:5188060/150‑1 (MQ=255)
                                      gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                            <  1:10844740/150‑1 (MQ=255)
                                      gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                            >  1:9079164/1‑150 (MQ=255)
                                      gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                            >  2:4446790/1‑150 (MQ=255)
                                       aTACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATg                                                                                                           >  1:7046404/1‑150 (MQ=255)
                                           ccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTg                                                                                                       >  1:3754358/1‑150 (MQ=255)
                                           ccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTg                                                                                                       <  1:6029327/150‑1 (MQ=255)
                                              cATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                    >  1:713647/1‑150 (MQ=255)
                                                tCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGga                                                                                                  >  1:1548143/1‑150 (MQ=255)
                                                   gcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaaga                                                                                               <  1:2024980/150‑1 (MQ=255)
                                                        agcGAGGCGACCAGGGTAGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGAGGTGACGGATGTCTGCGGGAAGAATAcc                                                                                          <  1:3453432/150‑1 (MQ=255)
                                                            aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                      >  2:8781008/1‑150 (MQ=255)
                                                             tgCGACCCGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAcc                                                                                     <  1:5088731/149‑1 (MQ=255)
                                                               cGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGa                                                                                   <  1:9754042/150‑1 (MQ=255)
                                                                 aCCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATc                                                                                 >  1:2504771/1‑150 (MQ=255)
                                                                  ccAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCa                                                                                >  2:10057580/1‑150 (MQ=255)
                                                                  ccAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCa                                                                                >  1:4326366/1‑150 (MQ=255)
                                                                    aGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                              >  2:4167844/1‑150 (MQ=255)
                                                                    aGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                              <  1:9285670/150‑1 (MQ=255)
                                                                    aGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                              <  1:8683111/150‑1 (MQ=255)
                                                                     gggTTGACGTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCa                                                                             <  2:10758348/150‑1 (MQ=255)
                                                                      ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAg                                                                            <  1:2630793/150‑1 (MQ=255)
                                                                      ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAg                                                                            <  1:10450432/150‑1 (MQ=255)
                                                                       gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                           >  2:2683712/1‑150 (MQ=255)
                                                                         tGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggc                                                                         <  1:811709/150‑1 (MQ=255)
                                                                         tGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggc                                                                         >  2:8327174/1‑150 (MQ=255)
                                                                         tGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggc                                                                         >  1:6243361/1‑150 (MQ=255)
                                                                          gACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcg                                                                        <  2:2214140/150‑1 (MQ=255)
                                                                             ttGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcg                                                                     >  1:10717851/1‑150 (MQ=255)
                                                                              tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcgg                                                                    >  2:9592907/1‑150 (MQ=255)
                                                                                  cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                <  2:2087429/150‑1 (MQ=255)
                                                                                  cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                <  2:4563817/150‑1 (MQ=255)
                                                                                   ccGATGCGGTCGCCGAAGTGTCCGAACAGAGCCGATCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAATGCGCTGATCATCTGCGCGGTTCCGGATTTCTTCGGGAAGAATACCGGACCGATCACCAGGGCGGCGTCAGTg                                                               <  2:341326/150‑1 (MQ=255)
                                                                                   ccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                               <  1:5849351/150‑1 (MQ=255)
                                                                                   ccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                               >  2:3129499/1‑150 (MQ=255)
                                                                                    cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                              <  2:3606443/150‑1 (MQ=255)
                                                                                     gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                             >  2:10498649/1‑150 (MQ=255)
                                                                                     gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                             <  2:5347044/150‑1 (MQ=255)
                                                                                      aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                            <  1:2372007/150‑1 (MQ=255)
                                                                                       tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                           >  2:162773/1‑150 (MQ=255)
                                                                                       tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                           <  2:5853035/150‑1 (MQ=255)
                                                                                        gCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                          <  2:9387106/150‑1 (MQ=255)
                                                                                         cGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAg                                                         <  2:797628/150‑1 (MQ=255)
                                                                                            tCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGCGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACg                                                      >  2:5539095/1‑150 (MQ=255)
                                                                                              gCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTa                                                    <  2:11034541/150‑1 (MQ=255)
                                                                                               ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                   <  1:8311554/150‑1 (MQ=255)
                                                                                                    gTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATc                                              <  2:5121261/150‑1 (MQ=255)
                                                                                                     tGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCg                                             <  2:9877517/150‑1 (MQ=255)
                                                                                                         cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGa                                         >  1:1825612/1‑150 (MQ=255)
                                                                                                         cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAGAGACGTAGAAATCGTAGa                                         >  2:473881/1‑150 (MQ=255)
                                                                                                          gAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGaa                                        <  1:10028421/150‑1 (MQ=255)
                                                                                                           aaCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAt                                       >  1:7662215/1‑150 (MQ=255)
                                                                                                            aCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAtt                                      <  1:5503796/150‑1 (MQ=255)
                                                                                                             cAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTc                                     <  2:9963065/150‑1 (MQ=255)
                                                                                                              aGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCg                                    <  1:4834944/150‑1 (MQ=255)
                                                                                                              aGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCg                                    <  2:6527575/150‑1 (MQ=255)
                                                                                                               gcgcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGa                                   >  1:4098454/1‑150 (MQ=255)
                                                                                                                  ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGGCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                >  1:7473464/1‑150 (MQ=255)
                                                                                                                  ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                <  2:8571634/150‑1 (MQ=255)
                                                                                                                   cGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGc                               <  1:3345455/150‑1 (MQ=255)
                                                                                                                    gAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCg                              >  1:4912764/1‑150 (MQ=255)
                                                                                                                     aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGTGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                             <  2:4062389/150‑1 (MQ=255)
                                                                                                                     aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                             >  1:10233687/1‑150 (MQ=255)
                                                                                                                      gCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGt                            <  1:3063470/150‑1 (MQ=255)
                                                                                                                         cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGcc                         <  2:6677624/150‑1 (MQ=255)
                                                                                                                         cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGcc                         >  1:849472/1‑150 (MQ=255)
                                                                                                                         cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGcc                         <  1:1413983/150‑1 (MQ=255)
                                                                                                                          aGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCg                        >  1:9498704/1‑150 (MQ=255)
                                                                                                                           gCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGa                       <  1:11018055/150‑1 (MQ=255)
                                                                                                                                cgagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAg                  >  1:4681768/1‑150 (MQ=255)
                                                                                                                                 gagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCGTAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                 <  2:6905768/150‑1 (MQ=255)
                                                                                                                                 gagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                 >  1:861920/1‑150 (MQ=255)
                                                                                                                                         aaGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCAGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATATACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACt         >  2:6218854/1‑150 (MQ=255)
                                                                                                                                          aGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTg        <  2:7425299/150‑1 (MQ=255)
                                                                                                                                               aTACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAcc   <  1:4526339/150‑1 (MQ=255)
                                                                                                                                               aTACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAcc   >  1:2126029/1‑150 (MQ=255)
                                                                                                                                               aTACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAcc   <  1:1671177/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑