Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,441,164 T→C I804I (ATT→ATC)  mexK → multidrug efflux RND transporter permease subunit MexK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,441,1640TC99.8% 1620.5 / ‑4.6 428I804I (ATT→ATC) mexKmultidrug efflux RND transporter permease subunit MexK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (204/223);  minor base A (1/0);  total (205/223)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 432 total aligned reads displayed.

GCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATTTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGCGG  >  NC_008463/1441017‑1441310
                                                                                                                                                   |                                                                                                                                                  
gCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTg                                                                                                                                                  >  1:4880355/1‑150 (MQ=255)
gCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTg                                                                                                                                                  >  1:10798278/1‑150 (MQ=255)
    aTCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCAc                                                                                                                                              <  2:3579875/150‑1 (MQ=255)
     tCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCAcc                                                                                                                                             <  1:9687325/150‑1 (MQ=255)
       gagaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGc                                                                                                                                           <  1:6185782/150‑1 (MQ=255)
       gagaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGc                                                                                                                                           >  2:5841996/1‑150 (MQ=255)
        agaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCa                                                                                                                                          >  2:3776093/1‑150 (MQ=255)
        agaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCa                                                                                                                                          >  1:3705642/1‑150 (MQ=255)
        agaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCa                                                                                                                                          >  1:7385746/1‑150 (MQ=255)
         gaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCaa                                                                                                                                         >  2:8126610/1‑150 (MQ=255)
         gaTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCaa                                                                                                                                         >  2:5719620/1‑150 (MQ=255)
          aTCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGCTCATCTGGCACCGCAAc                                                                                                                                        >  2:10622310/1‑150 (MQ=255)
           tcctccTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAAcc                                                                                                                                       >  1:3847415/1‑150 (MQ=255)
           tcctccTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAAcc                                                                                                                                       >  1:6347734/1‑150 (MQ=255)
            cctcctGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGACGAGGGGATCATCTGGCACCGCAACCg                                                                                                                                      >  2:10597356/1‑150 (MQ=255)
             ctcctGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGc                                                                                                                                     >  2:949202/1‑150 (MQ=255)
             ctcctGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGc                                                                                                                                     <  1:1948028/150‑1 (MQ=255)
               cctGCGCGTCACCGCGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCt                                                                                                                                   <  2:5503925/150‑1 (MQ=255)
                  gcgcGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGcc                                                                                                                                <  1:8084760/150‑1 (MQ=255)
                      ggCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACg                                                                                                                            >  2:4990468/1‑150 (MQ=255)
                         aCCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTc                                                                                                                         <  1:9430507/150‑1 (MQ=255)
                         aCCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTc                                                                                                                         <  1:5802267/150‑1 (MQ=255)
                           cGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCAc                                                                                                                       <  1:4323669/150‑1 (MQ=255)
                           cGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCGGGCGGTGCCGACCGAGACTGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCAc                                                                                                                       <  2:2186692/150‑1 (MQ=255)
                            gAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCAcc                                                                                                                      >  1:2044847/1‑150 (MQ=255)
                             aGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCg                                                                                                                     <  1:7960984/150‑1 (MQ=255)
                             aGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCg                                                                                                                     <  1:9328122/150‑1 (MQ=255)
                               cAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTg                                                                                                                   >  2:1760706/1‑150 (MQ=255)
                                 ggAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTgcg                                                                                                                 >  1:1391568/1‑150 (MQ=255)
                                    gCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTgcgcgc                                                                                                              <  2:10572363/150‑1 (MQ=255)
                                      gTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCg                                                                                                            >  2:6524131/1‑150 (MQ=255)
                                         gcgACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGata                                                                                                         <  1:5228662/150‑1 (MQ=255)
                                          cgACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGatat                                                                                                        <  1:4577948/150‑1 (MQ=255)
                                             ccTGTCGGTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTa                                                                                                     <  1:9512961/150‑1 (MQ=255)
                                               tGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTacg                                                                                                   >  1:2286453/1‑150 (MQ=255)
                                                gTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTacga                                                                                                  <  2:10223687/150‑1 (MQ=255)
                                                 tCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTacgac                                                                                                 >  1:8526751/1‑150 (MQ=255)
                                                 tCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTacgac                                                                                                 >  1:10508403/1‑150 (MQ=255)
                                                   ggtgctgcCGAGCCTGGCGGTGCCGACCGAGAAGGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATTTCTAcgacga                                                                                               <  2:1488253/148‑1 (MQ=255)
                                                     tgctgcCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGAtt                                                                                             <  1:6880214/150‑1 (MQ=255)
                                                      gctgcCGAGCCTGGCGGTGCCGACCGAGAATGGCAGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCGGCCGACGGTCACCGTGCGCGCCGATATATACGACGATTc                                                                                            >  2:1720683/1‑150 (MQ=255)
                                                          ccGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTg                                                                                        <  1:917736/150‑1 (MQ=255)
                                                          ccGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTg                                                                                        >  1:5510494/1‑150 (MQ=255)
                                                          ccGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTg                                                                                        <  1:1001100/150‑1 (MQ=255)
                                                          ccGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTg                                                                                        <  1:1704780/150‑1 (MQ=255)
                                                           cGAGCCTGGCTGTGCCGACCGAGAATGGCCGGAGCGTGGCTCTGTCGCAGATCTCCACTCTCGAATACTGTTTCGAAGAGGGGATCATCTGTCACCGAATAAGGCTGCCGAAGGTCAACGTGCGCGCCGATATCTACGTCGATTCTCTGc                                                                                       <  2:7406598/150‑1 (MQ=255)
                                                             aGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCg                                                                                     >  1:6686653/1‑150 (MQ=255)
                                                              gCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCgg                                                                                    <  2:390527/150‑1 (MQ=255)
                                                               ccTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGc                                                                                   <  2:3184090/150‑1 (MQ=255)
                                                                  ggCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGAc                                                                                <  2:3049990/150‑1 (MQ=255)
                                                                   gCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGAcc                                                                               >  1:8348428/1‑150 (MQ=255)
                                                                    cGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGAccc                                                                              <  2:9863706/150‑1 (MQ=255)
                                                                      gTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTg                                                                            >  1:2948563/1‑150 (MQ=255)
                                                                       tGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTgg                                                                           <  2:9693927/150‑1 (MQ=255)
                                                                       tGCCGACCGAGAATGGCCGGAGCGTGGAGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTgg                                                                           >  2:508996/1‑150 (MQ=255)
                                                                         ccgaccgaGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTc                                                                         <  1:2400076/150‑1 (MQ=255)
                                                                              cgaGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCa                                                                    <  2:1222325/150‑1 (MQ=255)
                                                                              cgaGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCACGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCa                                                                    >  2:3309463/1‑150 (MQ=255)
                                                                               gagaATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAg                                                                   >  1:1334399/1‑150 (MQ=255)
                                                                                agaATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGa                                                                  <  1:1506760/150‑1 (MQ=255)
                                                                                 gaATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGAt                                                                 <  2:9059785/150‑1 (MQ=255)
                                                                                       ccGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGcccc                                                           >  2:4334443/1‑150 (MQ=255)
                                                                                         ggAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGCTCATCTGGCACCGCAACCGCCTGCCGACGGTCCCCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGa                                                         >  2:7908149/1‑150 (MQ=255)
                                                                                         ggAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGa                                                         >  2:10067041/1‑150 (MQ=255)
                                                                                            gCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGAccc                                                      <  1:5071611/150‑1 (MQ=255)
                                                                                            gCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGAccc                                                      >  1:4493502/1‑150 (MQ=255)
                                                                                                   gcTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAAcc                                               <  1:10580776/150‑1 (MQ=255)
                                                                                                    cTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCg                                              >  2:8368591/1‑150 (MQ=255)
                                                                                                      gTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGAt                                            >  2:2938397/1‑150 (MQ=255)
                                                                                                        cGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATcc                                          <  2:8724970/150‑1 (MQ=255)
                                                                                                           aGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCcgcg                                       >  1:2488900/1‑150 (MQ=255)
                                                                                                                 ccACGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGc                                 <  2:9341036/150‑1 (MQ=255)
                                                                                                                    cGCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCCCCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGc                              <  2:7633343/150‑1 (MQ=255)
                                                                                                                     gCTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGcc                             >  1:8912192/1‑150 (MQ=255)
                                                                                                                      cTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCg                            >  1:9899609/1‑150 (MQ=255)
                                                                                                                      cTCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCg                            >  2:3097032/1‑150 (MQ=255)
                                                                                                                       tCGAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCgg                           >  2:6143599/1‑150 (MQ=255)
                                                                                                                         gAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGAc                         >  2:2368236/1‑150 (MQ=255)
                                                                                                                         gAATACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGAc                         >  1:7147196/1‑150 (MQ=255)
                                                                                                                           aTACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACgg                       >  1:1192140/1‑150 (MQ=255)
                                                                                                                           aTACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACgg                       <  2:2638518/150‑1 (MQ=255)
                                                                                                                            tACGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGc                      >  2:1043086/1‑150 (MQ=255)
                                                                                                                             aCGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCt                     >  1:3330678/1‑150 (MQ=255)
                                                                                                                             aCGGTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGAGTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCt                     <  2:8574757/150‑1 (MQ=255)
                                                                                                                                gTTTCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTAcc                  >  1:2157069/1‑150 (MQ=255)
                                                                                                                                 tttCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACct                 <  1:7535880/150‑1 (MQ=255)
                                                                                                                                 tttCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACct                 <  1:6060122/150‑1 (MQ=255)
                                                                                                                                  ttCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACctg                <  2:10817924/150‑1 (MQ=255)
                                                                                                                                   tCGAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACatgc               >  2:4657117/1‑150 (MQ=255)
                                                                                                                                     gAAGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACctgctg             >  2:7214131/1‑150 (MQ=255)
                                                                                                                                      aaGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTgg            >  2:6982818/1‑150 (MQ=255)
                                                                                                                                      aaGAGGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTgg            >  2:11069505/1‑150 (MQ=255)
                                                                                                                                       agagGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGa           >  1:10288622/1‑150 (MQ=255)
                                                                                                                                       agagGGGATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGa           >  1:3419049/1‑150 (MQ=255)
                                                                                                                                          ggggCTCATCTGGCACCGCACCCGCCTGCCGACGGTCACCGTGCGCGCCGAAATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGt        <  2:6858183/150‑1 (MQ=255)
                                                                                                                                          ggggATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGt        >  1:5978962/1‑150 (MQ=255)
                                                                                                                                          ggggATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGt        <  2:4959398/150‑1 (MQ=255)
                                                                                                                                             gATCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGTggg     >  1:1618596/1‑150 (MQ=255)
                                                                                                                                              atcatcTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGc    <  2:477585/150‑1 (MQ=255)
                                                                                                                                               tCATCTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGCg   <  1:1219813/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGTCGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATCGCCACGCTCGAATACGGTTTCGAAGAGGGGATCATTTGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGCGCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGCGCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGCGG  >  NC_008463/1441017‑1441310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑