Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,880,8720CG99.8% 2057.4 / NA 526A502G (GCC→GGC) PA14_RS08740DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (259/266);  total (260/266)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 537 total aligned reads displayed.

TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018
                                                                                                                                                   |                                                                                                                                                  
 ccGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgc                                                                                                                                                 >  2:10880950/1‑150 (MQ=255)
   gcgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                               >  1:9733789/1‑150 (MQ=255)
    cgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGcgcgcg                                                                                                                                              <  1:9338591/150‑1 (MQ=255)
     gcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCgg                                                                                                                                             >  1:5151437/1‑150 (MQ=255)
      ccGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTCTCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGc                                                                                                                                            <  1:398536/150‑1 (MQ=255)
      ccGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGc                                                                                                                                            <  1:8046822/150‑1 (MQ=255)
        gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                          >  2:3686305/1‑150 (MQ=255)
        gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                          >  2:5768678/1‑150 (MQ=255)
           cccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                       >  1:10764173/1‑150 (MQ=255)
           cccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCGATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                       >  2:6965038/1‑150 (MQ=255)
            ccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcg                                                                                                                                      >  1:6805646/1‑150 (MQ=255)
             cTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgc                                                                                                                                     >  2:10668960/1‑150 (MQ=255)
               aCGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCgcgc                                                                                                                                   >  2:5400179/1‑150 (MQ=255)
                cGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCt                                                                                                                                  <  1:7212787/150‑1 (MQ=255)
                  ccGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGa                                                                                                                                >  1:4581654/1‑150 (MQ=255)
                   cGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAc                                                                                                                               <  2:1527730/150‑1 (MQ=255)
                       cTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcg                                                                                                                           <  1:2848099/150‑1 (MQ=255)
                       cTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcg                                                                                                                           >  2:2910853/1‑150 (MQ=255)
                        tGGAGCGCGAGCGCTTCGACGCAGTCCTGCGGATGCTCGCCGAGGGCTACCACTGACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgg                                                                                                                          <  1:7714745/150‑1 (MQ=255)
                        tGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgg                                                                                                                          >  2:9140125/1‑150 (MQ=255)
                         ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCGCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                         >  1:5100786/1‑150 (MQ=255)
                          gagcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                        >  2:9287117/1‑150 (MQ=255)
                                gagcgcTACGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCCCGCCTCGGCGTGCGCGGCGCCTACCTCCAACGCGAGGCGCTCAACGGCCTGCTCCGCGGCCGCCGTGGCGCGCGGCTGACCGCGCTGCCCTCGGGCGGGAcc                                                                                                                  <  1:2052016/150‑1 (MQ=255)
                                 agcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCa                                                                                                                 <  2:3117350/150‑1 (MQ=255)
                                   cgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAtt                                                                                                               <  1:9941990/150‑1 (MQ=255)
                                    gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                              >  1:7070809/1‑150 (MQ=255)
                                     cTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTcc                                                                                                             <  2:2589052/150‑1 (MQ=255)
                                      ttCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTccc                                                                                                            >  2:412661/1‑150 (MQ=255)
                                           cgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                       <  1:6083563/150‑1 (MQ=255)
                                             aaGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCg                                                                                                     <  2:1047118/150‑1 (MQ=255)
                                              aGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                    <  2:1115557/150‑1 (MQ=255)
                                               gTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGc                                                                                                   <  1:7567342/150‑1 (MQ=255)
                                                  cTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGAc                                                                                                <  1:4409096/150‑1 (MQ=255)
                                                    gCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTa                                                                                              >  1:9534262/1‑150 (MQ=255)
                                                     cGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTAc                                                                                             <  1:10002855/150‑1 (MQ=255)
                                                       gATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAg                                                                                           <  2:2459706/150‑1 (MQ=255)
                                                       gATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAg                                                                                           <  2:7308011/150‑1 (MQ=255)
                                                         tGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCg                                                                                         <  1:8810422/150‑1 (MQ=255)
                                                               ccGAGGTCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCAGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                   <  1:5551902/150‑1 (MQ=255)
                                                                 gAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGctgctg                                                                                 <  2:2323168/150‑1 (MQ=255)
                                                                 gAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAGACCGGCGACTACAGCGtgctgcgg                                                                                 >  2:7170819/1‑148 (MQ=255)
                                                                  aGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                <  2:7479633/150‑1 (MQ=255)
                                                                  aGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                >  2:5951544/1‑150 (MQ=255)
                                                                  aGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                <  1:2343728/150‑1 (MQ=255)
                                                                   gggCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGa                                                                               >  1:3215419/1‑150 (MQ=255)
                                                                         ccACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCa                                                                         <  1:4947905/150‑1 (MQ=255)
                                                                           aCTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAgg                                                                       <  2:463938/150‑1 (MQ=255)
                                                                             tCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGc                                                                     <  2:6642804/150‑1 (MQ=255)
                                                                               aCGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                   <  2:1665905/150‑1 (MQ=255)
                                                                               aCGCCTCGGCGTGCGCGGCGCCTATATCCATCGCGATGCGCACAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCGGGAGCCGCAGGGccg                                                                   >  1:2952164/1‑149 (MQ=255)
                                                                                  cgtgggCTTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgct                                                                <  2:6361865/146‑1 (MQ=255)
                                                                                  ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgct                                                                <  2:837789/150‑1 (MQ=255)
                                                                                  ccTCGGCGTGCGCGGCGCCGATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCGGGAGCCGCAGGGCctgcg                                                                >  2:1922576/1‑149 (MQ=255)
                                                                                   cTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgctg                                                               <  2:8585802/150‑1 (MQ=255)
                                                                                    tCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGGAGACCGCGCGGACCACTGGCGCTACCATTCGCGGAACCGTCGACTACAGCTTGCTGCTTCAGACGCACGGActcagcg                                                              >  1:1512425/1‑145 (MQ=255)
                                                                                     cGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggt                                                             <  2:10099609/150‑1 (MQ=255)
                                                                                      ggCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggtg                                                            >  1:8668776/1‑150 (MQ=255)
                                                                                       gCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggtgg                                                           >  2:4265431/1‑150 (MQ=255)
                                                                                          tGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGaa                                                        >  2:3248629/1‑150 (MQ=255)
                                                                                             gcggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACgg                                                     <  1:1245734/150‑1 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCa                                                  >  1:9041340/1‑150 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  <  1:1352000/150‑1 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  >  1:6903293/1‑150 (MQ=255)
                                                                                                     tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAg                                             <  1:11014375/150‑1 (MQ=255)
                                                                                                     tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAg                                             >  1:4121574/1‑150 (MQ=255)
                                                                                                       tctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGa                                           >  2:6824410/1‑150 (MQ=255)
                                                                                                       tctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGa                                           >  2:39601/1‑150 (MQ=255)
                                                                                                          ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGACtt                                        >  2:135170/1‑150 (MQ=255)
                                                                                                          ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACtt                                        >  1:7833397/1‑150 (MQ=255)
                                                                                                          ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACtt                                        <  2:5078280/150‑1 (MQ=255)
                                                                                                             tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                     <  2:5532847/150‑1 (MQ=255)
                                                                                                             tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                     <  1:7975735/150‑1 (MQ=255)
                                                                                                              cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgcg                                    <  2:1763100/150‑1 (MQ=255)
                                                                                                              cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgcg                                    <  1:5869938/150‑1 (MQ=255)
                                                                                                               gcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCGCGGGCGGGACCATGCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCCGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                   >  2:5594697/1‑150 (MQ=255)
                                                                                                                  aTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTgg                                >  2:3622082/1‑150 (MQ=255)
                                                                                                                    gcgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgag                              <  1:1028417/150‑1 (MQ=255)
                                                                                                                     cgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                             <  1:3640548/150‑1 (MQ=255)
                                                                                                                      gcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                            <  2:8205248/150‑1 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGGGc                           >  2:9024000/1‑150 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGAGCCGCGCGGCTGAACGCCCTGACATCTCGCGTGACCAATCCAGATACCTCCGACGACAGCGTGCGGCAGGTGACGCAGTGCCTGCTGGTTGGAACGGTCAACGAGGACTTCGGGGTGGAGAGc                           >  1:3367241/1‑150 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGccg                         >  1:248379/1‑149 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         >  1:6320351/1‑150 (MQ=255)
                                                                                                                          aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTg                        >  2:8849793/1‑150 (MQ=255)
                                                                                                                          aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCGGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAGGACTTCGCGGTGGAGCGCCTg                        >  2:10216219/1‑150 (MQ=255)
                                                                                                                            cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGc                      <  1:8226694/150‑1 (MQ=255)
                                                                                                                            cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGc                      <  1:6601999/150‑1 (MQ=255)
                                                                                                                            cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGc                      <  2:7912090/150‑1 (MQ=255)
                                                                                                                               ccTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCgg                   >  2:1598016/1‑150 (MQ=255)
                                                                                                                               ccTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCgg                   >  1:5403148/1‑150 (MQ=255)
                                                                                                                                cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGc                  <  2:4473768/150‑1 (MQ=255)
                                                                                                                                cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGc                  >  1:1141193/1‑150 (MQ=255)
                                                                                                                                  gCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGa                <  1:3872785/150‑1 (MQ=255)
                                                                                                                                    tCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACg              <  1:183334/150‑1 (MQ=255)
                                                                                                                                      cGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtg            >  1:2715054/1‑150 (MQ=255)
                                                                                                                                       gTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtgt           >  1:10584459/1‑150 (MQ=255)
                                                                                                                                         ggCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTc         <  2:9679363/150‑1 (MQ=255)
                                                                                                                                         ggCCGCCGTGGCGCGCGGCTGACCGCGCTGACCGCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGTGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTc         >  2:4846488/1‑150 (MQ=255)
                                                                                                                                           ccgccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCa       <  1:9183028/150‑1 (MQ=255)
                                                                                                                                               cgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg   >  1:4520345/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑