Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,762,030 A→G K127E (AAG→GAG)  PA14_RS12995 → lysophospholipid acyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,762,0300AG100.0% 1653.7 / NA 424K127E (AAG→GAG) PA14_RS12995lysophospholipid acyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (207/217);  total (207/217)

Only 100 of 424 total aligned reads displayed.

ACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761884‑2762170
                                                                                                                                                  |                                                                                                                                            
aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           <  1:7958818/150‑1 (MQ=255)
aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           <  1:746221/150‑1 (MQ=255)
 cGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          <  2:7149014/150‑1 (MQ=255)
  gTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTc                                                                                                                                         <  2:8157108/150‑1 (MQ=255)
   tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        <  2:2379658/150‑1 (MQ=255)
   tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        <  1:7835474/150‑1 (MQ=255)
         acaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGt                                                                                                                                  >  1:6246615/1‑150 (MQ=255)
         acaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGt                                                                                                                                  >  2:10178864/1‑150 (MQ=255)
          caaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGtt                                                                                                                                 <  1:2969103/150‑1 (MQ=255)
          caaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGtt                                                                                                                                 >  1:7992194/1‑150 (MQ=255)
            aGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGc                                                                                                                               >  1:1155749/1‑150 (MQ=255)
            aGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGc                                                                                                                               >  1:4882963/1‑150 (MQ=255)
             ggTGTTCCACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGcc                                                                                                                              <  2:2818347/150‑1 (MQ=255)
               tgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAt                                                                                                                            <  1:5984501/150‑1 (MQ=255)
                 ttCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTc                                                                                                                          >  1:10682759/1‑150 (MQ=255)
                 ttCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTc                                                                                                                          >  1:6676183/1‑150 (MQ=255)
                  tCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCa                                                                                                                         >  1:6486994/1‑150 (MQ=255)
                    aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       >  2:5592619/1‑150 (MQ=255)
                    aaCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAag                                                                                                                       <  2:7028236/150‑1 (MQ=255)
                     aCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAaga                                                                                                                      >  2:7663946/1‑150 (MQ=255)
                     aCGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAaga                                                                                                                      >  2:8085364/1‑150 (MQ=255)
                       ggCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGc                                                                                                                    <  1:2725502/150‑1 (MQ=255)
                         cGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCgg                                                                                                                  <  1:9490902/150‑1 (MQ=255)
                          gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 >  1:2331045/1‑150 (MQ=255)
                          gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 <  1:4632763/150‑1 (MQ=255)
                          gTGCTGGTCGACCGCCAGCGCAGCGAGGCCAATGCGAATCCGCACGAGGCCCTCGACCGCGCCCAGTACGGCGGCGATTCGCTGATCATCATCCCCGGGGGCACCGGCAACCTGGACGTCGAGGTCAAGCTGTTGCGAGTCAGGAGCGGt                                                                                                                 >  2:136216/1‑150 (MQ=255)
                           tGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTa                                                                                                                <  2:9571692/150‑1 (MQ=255)
                           tGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTa                                                                                                                >  2:7479913/1‑150 (MQ=255)
                            gCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTAt                                                                                                               <  1:7019265/150‑1 (MQ=255)
                                  cGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTacca                                                                                                         <  1:4166032/150‑1 (MQ=255)
                                   gACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccac                                                                                                        <  2:5482065/150‑1 (MQ=255)
                                       gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGACCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    <  2:4600154/150‑1 (MQ=255)
                                       gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    <  2:658014/150‑1 (MQ=255)
                                       gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    <  1:3072625/150‑1 (MQ=255)
                                        ccAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTc                                                                                                   >  1:135592/1‑150 (MQ=255)
                                        ccAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTc                                                                                                   >  2:6878883/1‑150 (MQ=255)
                                          agcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTccc                                                                                                 <  2:6701950/150‑1 (MQ=255)
                                          agcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTccc                                                                                                 >  2:6041806/1‑150 (MQ=255)
                                          agcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTccc                                                                                                 <  2:8713527/150‑1 (MQ=255)
                                              cagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGc                                                                                             >  2:111319/1‑150 (MQ=255)
                                                 cgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCa                                                                                          <  2:5268292/150‑1 (MQ=255)
                                                    tgccaatgccaaTCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAccc                                                                                       >  1:6340459/1‑150 (MQ=255)
                                                       caatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGa                                                                                    <  1:1443342/150‑1 (MQ=255)
                                                        aatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAg                                                                                   >  1:216288/1‑150 (MQ=255)
                                                              aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             >  2:2573852/1‑150 (MQ=255)
                                                              aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             <  1:1804248/150‑1 (MQ=255)
                                                               atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            >  1:7379866/1‑150 (MQ=255)
                                                               atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            >  2:10410384/1‑150 (MQ=255)
                                                                tCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCt                                                                           >  1:4697978/1‑150 (MQ=255)
                                                                 ccGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTg                                                                          >  2:9377644/1‑150 (MQ=255)
                                                                        aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  1:8089424/1‑150 (MQ=255)
                                                                        aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  1:5012907/1‑150 (MQ=255)
                                                                          ccccccGACCGCGCCCCGGACGGCGCCGATTCGCCGATCATCTTCCCCGAGGGCACCCTCAACCTGGACGACGAGGACAAGCTGTTGCCATTCACGAGCGGTATCTACCACCTGGCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTc                                                                 <  2:4175359/150‑1 (MQ=255)
                                                                            ccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctg                                                               <  2:3838968/150‑1 (MQ=255)
                                                                            ccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGGTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctg                                                               <  2:2582645/150‑1 (MQ=255)
                                                                               cGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggct                                                            <  2:10990869/150‑1 (MQ=255)
                                                                                 aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  2:3482010/1‑150 (MQ=255)
                                                                                 aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  2:4829418/1‑150 (MQ=255)
                                                                                   cgcgCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGcc                                                        >  1:3852993/1‑150 (MQ=255)
                                                                                      gcCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAAc                                                     >  2:5768456/1‑150 (MQ=255)
                                                                                         cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  <  2:8261342/150‑1 (MQ=255)
                                                                                         cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  <  2:7758100/150‑1 (MQ=255)
                                                                                          tGGACGGCGGCGATTCGCTGATCATCTTCCCCGATGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGCGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTct                                                 <  1:5330490/150‑2 (MQ=255)
                                                                                          tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  2:6163539/1‑150 (MQ=255)
                                                                                              cggcggCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCg                                             >  1:3413386/1‑150 (MQ=255)
                                                                                               ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            >  1:10421566/1‑150 (MQ=255)
                                                                                               ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            >  1:8807206/1‑150 (MQ=255)
                                                                                                gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  2:1853851/1‑150 (MQ=255)
                                                                                                gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  1:5656769/1‑150 (MQ=255)
                                                                                                 cggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGt                                          <  2:4009958/150‑1 (MQ=255)
                                                                                                  ggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTc                                         <  2:159189/150‑1 (MQ=255)
                                                                                                   gcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCa                                        >  2:4990028/1‑150 (MQ=255)
                                                                                                   gcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCa                                        >  1:4320447/1‑150 (MQ=255)
                                                                                                    cgAGTCGCTGATCATCGTCCCCGAGGGCACCCGCAACCTGGACGACGTGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGGTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCAt                                       <  2:8605111/150‑1 (MQ=255)
                                                                                                     gATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATg                                      <  1:1945166/150‑1 (MQ=255)
                                                                                                      aTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGc                                     >  1:8673666/1‑150 (MQ=255)
                                                                                                       ttCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGcc                                    >  2:7877290/1‑150 (MQ=255)
                                                                                                       ttCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGcc                                    >  2:9047756/1‑150 (MQ=255)
                                                                                                        tCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGccc                                   >  2:7192071/1‑150 (MQ=255)
                                                                                                          gCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCaa                                 >  2:4366668/1‑150 (MQ=255)
                                                                                                            tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               <  2:6648852/150‑1 (MQ=255)
                                                                                                            tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               <  1:9308093/150‑1 (MQ=255)
                                                                                                                 atcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGc                          <  1:8758372/150‑1 (MQ=255)
                                                                                                                  tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCa                         <  1:5408113/150‑1 (MQ=255)
                                                                                                                   cTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAt                        >  1:2822041/1‑150 (MQ=255)
                                                                                                                    ttCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtg                       <  1:1262408/150‑1 (MQ=255)
                                                                                                                     tCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgc                      <  2:2289089/150‑1 (MQ=255)
                                                                                                                       cccGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgctg                    <  1:8475770/150‑1 (MQ=255)
                                                                                                                            gggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGtt               >  1:10112978/1‑150 (MQ=255)
                                                                                                                             ggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGTGCCGCATGCTGCCGTTg              >  1:10233158/1‑150 (MQ=255)
                                                                                                                              gCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGc             <  1:7178079/150‑1 (MQ=255)
                                                                                                                              gCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGc             <  1:5194778/150‑1 (MQ=255)
                                                                                                                                aCCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCg           >  2:9863997/1‑150 (MQ=255)
                                                                                                                                 cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          <  1:9020009/150‑1 (MQ=255)
                                                                                                                                 cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          >  2:8871470/1‑150 (MQ=255)
                                                                                                                                    gCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgc       >  2:3240604/1‑150 (MQ=255)
                                                                                                                                    gCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgc       >  1:10004068/1‑150 (MQ=255)
                                                                                                                                     cAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgct      >  2:9776521/1‑150 (MQ=255)
                                                                                                                                       aCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGctct    <  1:969037/150‑1 (MQ=255)
                                                                                                                                         cTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGc  >  2:6320142/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
ACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761884‑2762170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑