BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,730,681	2 bp→CG	intergenic (+19/+95)	PA14_RS12860 → / ← ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,730,681	0	G	C	99.7%	1295.7 / NA	343	intergenic (+19/+96)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (146/196); minor base A (1/0); total (147/196)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	2,730,682	0	C	G	99.7%	1319.9 / NA	343	intergenic (+20/+95)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (147/195); minor base T (0/1); total (147/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGG  >  NC_008463/2730537‑2730822

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 gccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATa                                                                                                                                         <  2:3584011/150‑1 (MQ=255)

   cgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                       <  1:8088995/150‑1 (MQ=255)

   cgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                       <  1:6014078/150‑1 (MQ=255)

   cgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                       <  1:2440503/150‑1 (MQ=255)

    gccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgc                                                                                                                                      <  2:10056428/150‑1 (MQ=255)

     ccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                     >  1:2165848/1‑150 (MQ=255)

     ccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                     >  2:1062775/1‑150 (MQ=255)

     ccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCAGAGgcg                                                                                                                                     >  2:10928462/1‑150 (MQ=255)

        gCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                  >  1:1359031/1‑150 (MQ=255)

         cTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTAt                                                                                                                                 >  2:7921967/1‑150 (MQ=255)

             gCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGTGTAGCGCCATAGGCGCTATCCGc                                                                                                                             <  2:33944/150‑1 (MQ=255)

                ggCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAg                                                                                                                          >  2:623616/1‑150 (MQ=255)

                 gCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAgg                                                                                                                         >  2:7584078/1‑150 (MQ=255)

                 gCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAgg                                                                                                                         >  1:5625998/1‑150 (MQ=255)

                    gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGTCCTGCTGGTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACATCGCTGTCGACTGTGCGGGCCAGGGCGGGTAGCGCCATTGGCGCTATCCGCCAGGccc                                                                                                                      >  2:2573767/1‑150 (MQ=255)

                    gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGccc                                                                                                                      <  2:3769824/150‑1 (MQ=255)

                     ccGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGcccc                                                                                                                     <  2:6464763/150‑1 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGATCTCGACTGTGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCg                                                                                                                    <  1:9578545/150‑1 (MQ=255)

                       gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGt                                                                                                                   <  1:837129/150‑1 (MQ=255)

                       gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGt                                                                                                                   <  1:7695273/150‑1 (MQ=255)

                           tACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGa                                                                                                               <  1:729086/150‑1 (MQ=255)

                           tACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGAGCCATAGGAGCTATCAGCCAGGCCCCGTTGGa                                                                                                               >  2:2488827/1‑150 (MQ=255)

                             cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                             <  2:5942699/150‑1 (MQ=255)

                             cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                             <  1:8789876/150‑1 (MQ=255)

                              gacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACACCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTg                                                                                                            <  2:1880404/150‑1 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGGGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                          <  2:1048686/150‑1 (MQ=255)

                                 ggCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                         >  2:5227317/1‑150 (MQ=255)

                                  gCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCgg                                                                                                        <  1:846915/150‑1 (MQ=255)

                                   cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACACCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                       <  2:27734/150‑1 (MQ=255)

                                   cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                       >  2:3906076/1‑150 (MQ=255)

                                   cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                       >  2:7030002/1‑150 (MQ=255)

                                    tACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAt                                                                                                      <  2:7265805/150‑1 (MQ=255)

                                      cGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATaa                                                                                                    >  1:1255022/1‑150 (MQ=255)

                                        aGTGGATGCAGGCCGTCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAcc                                                                                                  <  2:5382249/150‑1 (MQ=255)

                                        aGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCCGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAcc                                                                                                  >  1:6333038/1‑150 (MQ=255)

                                         gTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCg                                                                                                 <  2:5473668/150‑1 (MQ=255)

                                            gATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGGGGCGACCGGGCTGATCTACGATGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtgt                                                                                              <  1:9210267/150‑1 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGTTAGCGCCATATGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                             <  1:4141238/150‑1 (MQ=255)

                                                 aGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcgc                                                                                         >  1:10023084/1‑150 (MQ=255)

                                                 aGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcgc                                                                                         <  2:915021/150‑1 (MQ=255)

                                                  ggCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCt                                                                                        <  2:500986/150‑1 (MQ=255)

                                                  ggCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCt                                                                                        >  1:9293280/1‑150 (MQ=255)

                                                   gCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCtt                                                                                       <  1:5003865/150‑1 (MQ=255)

                                                     cGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGt                                                                                     <  2:6194894/150‑1 (MQ=255)

                                                        cACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATCGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCg                                                                                  <  1:9863755/150‑1 (MQ=255)

                                                        cACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCg                                                                                  <  1:6575388/150‑1 (MQ=255)

                                                          ccGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGcc                                                                                <  2:9569674/150‑1 (MQ=255)

                                                           cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGccc                                                                               <  2:10152568/150‑1 (MQ=255)

                                                             aCCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTa                                                                             <  2:9429245/150‑1 (MQ=255)

                                                              ccTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTAc                                                                            <  2:1436984/150‑1 (MQ=255)

                                                               ctgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTAcc                                                                           >  1:36911/1‑150 (MQ=255)

                                                                tgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCt                                                                          <  1:8387488/150‑1 (MQ=255)

                                                                 gctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTc                                                                         <  1:4278400/150‑1 (MQ=255)

                                                                  ctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCg                                                                        <  1:867126/150‑1 (MQ=255)

                                                                    gctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTg                                                                      >  2:8580795/1‑150 (MQ=255)

                                                                      tggtggCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGa                                                                    <  2:1945172/150‑1 (MQ=255)

                                                                       ggtggcggtggcGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGaa                                                                   <  1:1951848/150‑1 (MQ=255)

                                                                        gtggcggtggcgACCGGGCTGATCTACTATGTGCTGGACAACGCTCTCGACTGAGCGGGCCGGGGCGGGTAGCGCCATTGGCGCTTTCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAg                                                                  <  1:2819950/150‑1 (MQ=255)

                                                                         tggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGt                                                                 <  2:172934/150‑1 (MQ=255)

                                                                            cggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCt                                                              <  2:6024447/150‑1 (MQ=255)

                                                                             ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtt                                                             <  2:10569911/150‑1 (MQ=255)

                                                                              gtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                            >  1:2495252/1‑150 (MQ=255)

                                                                               tggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtttt                                                           <  1:7369778/150‑1 (MQ=255)

                                                                                   gACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTa                                                       <  1:6074237/150‑1 (MQ=255)

                                                                                    aCCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                      >  1:10290302/1‑150 (MQ=255)

                                                                                     ccGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcc                                                     >  2:6800976/1‑150 (MQ=255)

                                                                                       gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcccc                                                   >  1:339025/1‑150 (MQ=255)

                                                                                       gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcccc                                                   >  2:3162102/1‑150 (MQ=255)

                                                                                       gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcccc                                                   <  2:1221803/150‑1 (MQ=255)

                                                                                        ggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                  >  2:6577365/1‑150 (MQ=255)

                                                                                        ggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                  <  2:4090211/150‑1 (MQ=255)

                                                                                          cTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAgg                                                <  2:10974047/150‑1 (MQ=255)

                                                                                            gATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTc                                              >  1:4429660/1‑150 (MQ=255)

                                                                                             aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCa                                             >  2:8080447/1‑150 (MQ=255)

                                                                                             aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCa                                             >  2:4724903/1‑150 (MQ=255)

                                                                                                tACGAAGTGCTGGACAACGCTCTCTACTGAGCGTGCAATGGCGGTTAGCGCCATAGGCGCTATACTCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAATTCCTTTTCTTACCCCAGTTAAGGc                                          <  1:6453977/150‑1 (MQ=255)

                                                                                                 aCGAAGTGCTGGACAACGCTCTCGACTGCGCGGGCCTGGGCGGGAAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCATTTCTTACCCCAGGTCAGGcc                                         <  1:4580290/150‑1 (MQ=255)

                                                                                                  cGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGccc                                        >  1:5663794/1‑150 (MQ=255)

                                                                                                  cGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGccc                                        >  1:6770898/1‑150 (MQ=255)

                                                                                                  cGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCACGTGGAAGTCCTGTTCTTACCCCAGGGAAGGccc                                        >  1:4019864/1‑150 (MQ=255)

                                                                                                   gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCg                                       <  1:6224058/150‑1 (MQ=255)

                                                                                                    aaGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTGTTCTTACCCCAGGTCAGGCCCGc                                      >  2:896982/1‑150 (MQ=255)

                                                                                                     aGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCa                                     <  1:10178356/150‑1 (MQ=255)

                                                                                                      gTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAg                                    >  2:8866253/1‑150 (MQ=255)

                                                                                                           ggACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGtt                               <  1:5510595/150‑1 (MQ=255)

                                                                                                             aCAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                             >  1:2709293/1‑150 (MQ=255)

                                                                                                              cAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCa                            <  1:5062451/150‑1 (MQ=255)

                                                                                                               aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAg                           >  1:10895978/1‑150 (MQ=255)

                                                                                                                  gCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGc                        >  2:7766760/1‑150 (MQ=255)

                                                                                                                   ctctCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCa                       <  2:290896/150‑1 (MQ=255)

                                                                                                                    tctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCAt                      <  2:10282858/150‑1 (MQ=255)

                                                                                                                     ctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATc                     >  1:2040859/1‑150 (MQ=255)

                                                                                                                      tcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCg                    <  2:4333617/150‑1 (MQ=255)

                                                                                                                       cGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGc                   >  1:8266906/1‑150 (MQ=255)

                                                                                                                           tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAg               <  1:9650259/150‑1 (MQ=255)

                                                                                                                            gAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt              >  1:1117739/1‑150 (MQ=255)

                                                                                                                               cGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgc           <  1:7009186/150‑1 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGcgcg        <  1:6503921/150‑1 (MQ=255)

                                                                                                                                   ccAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGACGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCCTCGCCCAGTTGCGCgg       >  2:4456073/1‑150 (MQ=255)

                                                                                                                                        gcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAgg  <  2:8266970/150‑1 (MQ=255)

||

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGG  >  NC_008463/2730537‑2730822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`