BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,358,513	Δ1 bp	pseudogene (774/1285 nt)	PA14_RS24540 →	site‑specific integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,358,510	0	T	.	100.0%	576.3 / NA	285	pseudogene (771/1285 nt)	PA14_RS24540	site‑specific integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (139/146); total (139/146)

Only 100 of 299 total aligned reads displayed.

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCCACCGGTTCTCACCGCACTCCAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGGTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    >  2:8723845/1‑151 (MQ=9)

cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    >  1:4547752/1‑151 (MQ=31)

   tAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACCAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttc                                                                                                                                                 <  1:513409/151‑2 (MQ=14)

   tAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttc                                                                                                                                                 <  1:9651154/151‑2 (MQ=14)

     gagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                               >  2:1074221/1‑148 (MQ=1)

     gagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                               >  2:6527693/1‑148 (MQ=1)

                                                    tcACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                               >  2:8898312/1‑151 (MQ=1)

                                                    tcACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                               <  1:8176557/151‑1 (MQ=1)

                                                     cACCGCACTACAAGAGCAAGGTTATGCACTCCTACCAGGTCGAAGCCATGTACCTGAAGTTGACCGAGAAGGTTGTGGTTCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              <  2:9650245/151‑1 (MQ=11)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              <  1:10097362/151‑1 (MQ=11)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTACGGCACACCCTGGCCTCCGACTTGATGTAGGCACCCGAGCGGAACATTCa                                                                                              <  1:8341117/151‑1 (MQ=2)

                                                       ccGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAcc                                                                                            <  2:7142581/151‑1 (MQ=11)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           >  1:4806039/1‑151 (MQ=11)

                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         >  2:6211729/1‑151 (MQ=11)

                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         <  1:10536462/151‑1 (MQ=11)

                                                           aCTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCAc                                                                                        <  1:5791878/151‑1 (MQ=11)

                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       <  1:6389323/151‑1 (MQ=11)

                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       >  2:519057/1‑151 (MQ=11)

                                                              aCAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGaa                                                                                     <  1:542898/151‑1 (MQ=11)

                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   >  1:2352934/1‑151 (MQ=11)

                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   <  1:5243237/151‑1 (MQ=11)

                                                                aaGAGCAAGGTGATGAACTCCGAACAGGTCGAAGCCATGTACCGTAAGTAGACAGAGAAGCTTGGGGTGCGGATGACCCCGCACCG‑ATTCCGGCACACCCAGGCCACCGACTAGATGAACGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   >  1:3057891/1‑151 (MQ=11)

                                                                 agagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTg                                                                                  >  2:5675658/1‑151 (MQ=11)

                                                                 agagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTg                                                                                  <  1:803335/151‑1 (MQ=11)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               >  2:1308979/1‑151 (MQ=11)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               >  1:1400121/1‑151 (MQ=11)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGACGCCTTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAACTGCCTg                                                                              <  2:9246577/151‑1 (MQ=2)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTg                                                                              >  2:1174552/1‑151 (MQ=11)

                                                                      aaGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGc                                                                             >  1:7476531/1‑151 (MQ=11)

                                                                       aGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCt                                                                            <  1:7877549/151‑1 (MQ=11)

                                                                        ggTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTc                                                                           >  1:754961/1‑151 (MQ=11)

                                                                         gTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                          >  1:9245376/1‑151 (MQ=11)

                                                                         gTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                          >  1:3143593/1‑151 (MQ=11)

                                                                             tgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCa                                                                      >  2:9356250/1‑151 (MQ=11)

                                                                              gaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCAc                                                                     >  2:741000/1‑151 (MQ=11)

                                                                               aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGACCATTCACCTCACGAAGTGCCTGCTCAACCACt                                                                    >  2:5301780/1‑151 (MQ=2)

                                                                               aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACt                                                                    <  1:10085350/151‑1 (MQ=11)

                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCg                                                                  <  2:6329644/151‑1 (MQ=11)

                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCg                                                                  <  1:1924206/151‑1 (MQ=11)

                                                                                      aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAatat                                                             >  2:8969271/1‑151 (MQ=11)

                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  1:7467663/1‑151 (MQ=11)

                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          <  2:447560/151‑1 (MQ=11)

                                                                                          ggTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATTTCCAg                                                         >  1:7660031/1‑151 (MQ=2)

                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     >  1:3952099/1‑151 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  2:4369074/151‑1 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  1:5715504/151‑1 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  1:7271484/151‑1 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  2:8169362/151‑1 (MQ=11)

                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    <  1:9428050/151‑1 (MQ=11)

                                                                                                 gCCATGTACCGGGAGTTGCCCGAGAAGGTTGGGGTGCGGATGCCCCCGCACCG‑GATCCGGCACACCCAGGCCCCCGACTTGAAGAAGGCACCCGAGCGGAACATTCACCTCACGAACTGCCAGCTCAACCACTCGAAAATCCAGACCACGa                                                  >  2:1859155/1‑151 (MQ=11)

                                                                                                 gCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGa                                                  >  2:9829944/1‑151 (MQ=14)

                                                                                                  ccATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGCTGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGag                                                 >  2:6631325/1‑150 (MQ=9)

                                                                                                   cATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATg                                                <  2:4586953/151‑1 (MQ=17)

                                                                                                    aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGa                                               <  1:9600124/151‑1 (MQ=17)

                                                                                                    aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGa                                               >  1:4313591/1‑151 (MQ=17)

                                                                                                     tGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGGCTGCTCAACCACTCGAATATCCAGACCACGATGAg                                              <  1:10409366/151‑1 (MQ=12)

                                                                                                      gTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGc                                             >  2:6152820/1‑151 (MQ=17)

                                                                                                       tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                            <  2:3095295/151‑1 (MQ=17)

                                                                                                         ccGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTAc                                          >  2:7069744/1‑151 (MQ=17)

                                                                                                         ccGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTAc                                          <  2:8428619/151‑1 (MQ=17)

                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  2:3269123/151‑1 (MQ=17)

                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  1:7310640/151‑1 (MQ=17)

                                                                                                           ggAAGGTGACCGAGAAGGTTGGGGAGCGGCTGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  2:7547466/151‑1 (MQ=255)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      <  1:2672970/151‑1 (MQ=17)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      >  2:7581315/1‑151 (MQ=17)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      <  1:8848825/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  2:778605/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     >  2:4201225/1‑151 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTCGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  1:5872795/151‑1 (MQ=12)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    >  1:2592541/1‑151 (MQ=17)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    <  2:9466011/151‑1 (MQ=17)

                                                                                                                 tGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGc                                  >  2:2803541/1‑151 (MQ=17)

                                                                                                                  gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                 <  1:4661913/151‑1 (MQ=17)

                                                                                                                   aCCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCg                                <  2:3963948/151‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               >  1:10298353/1‑151 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  1:485339/151‑1 (MQ=17)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             <  1:2248266/151‑1 (MQ=17)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  1:3918226/1‑151 (MQ=17)

                                                                                                                       agaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTa                            >  2:3774015/1‑151 (MQ=17)

                                                                                                                       agaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTa                            <  1:8656153/151‑1 (MQ=17)

                                                                                                                         aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGTCTACg                          >  1:1686792/1‑151 (MQ=12)

                                                                                                                         aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACg                          >  1:2845919/1‑151 (MQ=17)

                                                                                                                          aGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGa                         >  2:10407980/1‑151 (MQ=17)

                                                                                                                           ggTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGAt                        >  1:6000467/1‑151 (MQ=18)

                                                                                                                            gTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATc                       <  2:4970916/151‑1 (MQ=18)

                                                                                                                             ttGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATca                      <  1:8601981/151‑1 (MQ=21)

                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   >  1:9304099/1‑151 (MQ=21)

                                                                                                                                   tGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcg                <  1:6840059/151‑1 (MQ=21)

                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              <  2:2717682/151‑1 (MQ=21)

                                                                                                                                      ggATGACCCCGCACCG‑TAACAGGCACAACCAGGCCACCGACTTGATGAAGGCACCCGAGCGGAACAATCAACTCACGAATTGCCAGCTCAACCACTCGAATATCCAGACAACGATGAGCTCCATCTAGCCCGACTACGAAcacaatccagc             >  2:6964792/1‑144 (MQ=11)

                                                                                                                                       gATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgcc            <  1:6263924/151‑1 (MQ=21)

                                                                                                                                       gATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgcc            >  1:6576052/1‑151 (MQ=21)

                                                                                                                                         tGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgt          <  1:10719092/151‑1 (MQ=21)

                                                                                                                                          gACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgtg         <  1:827883/151‑1 (MQ=21)

                                                                                                                                            ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgct       <  2:4803031/151‑1 (MQ=18)

                                                                                                                                               cGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa    <  1:5570316/151‑1 (MQ=17)

                                                                                                                                               cGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa    <  1:7054216/151‑1 (MQ=17)

                                                                                                                                                gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAt   >  1:9740090/1‑151 (MQ=17)

                                                                                                                                                 cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATg  >  2:8023374/1‑151 (MQ=17)

                                                                                                                                                 cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATg  >  2:8853092/1‑151 (MQ=17)

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`