Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,514,060 (G)5→4 intergenic (+15/+136) PA14_RS25260 → / ← PA14_RS25265 tRNA‑Met/HigA family addiction module antitoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,514,0560G.98.7% 1832.8 / 11.3 378intergenic (+11/+140)PA14_RS25260/PA14_RS25265tRNA‑Met/HigA family addiction module antitoxin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (193/180);  minor base T (4/1);  total (197/181)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCCG  >  NC_008463/5513906‑5514202
                                                                                                                                                      |                                                                                                                                                  
aGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCTAg                                                                                                                                                    >  1:9696089/1‑151 (MQ=255)
   aaaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAgggg                                                                                                                                                 >  1:5253409/1‑151 (MQ=255)
    caTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCCCTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggt                                                                                                                                                <  1:8176147/150‑2 (MQ=255)
      ttATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggtta                                                                                                                                              >  1:3711933/1‑148 (MQ=255)
      ttATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggtta                                                                                                                                              <  1:3464042/151‑4 (MQ=255)
        aTCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgc                                                                                                                                           <  2:3314097/151‑1 (MQ=255)
        aTCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgc                                                                                                                                           >  2:1690499/1‑151 (MQ=255)
         tCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgcg                                                                                                                                          >  2:5977217/1‑151 (MQ=255)
          cTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgcgc                                                                                                                                         >  2:9411735/1‑151 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCAGCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                        >  1:736825/1‑151 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                        <  2:9665132/151‑1 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                        >  2:4410625/1‑151 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                        >  2:2321256/1‑151 (MQ=255)
            aGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCaa                                                                                                                                       >  2:3534595/1‑151 (MQ=255)
            aGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTC‑ATGGGGTTAGCGCaa                                                                                                                                       >  2:8439739/1‑151 (MQ=255)
             ggCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAg                                                                                                                                      >  1:2103964/1‑151 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                    <  1:1631444/151‑1 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                    >  2:632610/1‑151 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                    >  1:6262583/1‑151 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                    <  1:9007437/151‑1 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                    >  1:2310231/1‑151 (MQ=255)
                 cAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTaa                                                                                                                                  >  2:6839660/1‑151 (MQ=255)
                 cAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTaa                                                                                                                                  >  1:7149084/1‑151 (MQ=255)
                   ggggTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAAcc                                                                                                                                >  2:9247144/1‑151 (MQ=255)
                    gggTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAAccc                                                                                                                               >  1:1399858/1‑151 (MQ=255)
                        tGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttt                                                                                                                           >  1:3250096/1‑151 (MQ=255)
                         gACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCtttt                                                                                                                          <  1:8550105/151‑1 (MQ=255)
                           cAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttg                                                                                                                        <  1:1056063/151‑1 (MQ=255)
                             gCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtt                                                                                                                      >  2:2478621/1‑151 (MQ=255)
                               cccgcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtttt                                                                                                                    >  2:582203/1‑151 (MQ=255)
                                   cccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTc                                                                                                                >  2:4931720/1‑151 (MQ=255)
                                   cccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTc                                                                                                                <  1:5011982/151‑1 (MQ=255)
                                   cccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTc                                                                                                                >  1:10232543/1‑151 (MQ=255)
                                    ccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCg                                                                                                               >  1:4117751/1‑151 (MQ=255)
                                         aTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCGAACCCCTTTTTGTTTTGGTCGGCAga                                                                                                          >  1:5398961/1‑151 (MQ=255)
                                             ggAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGGTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactac                                                                                                      >  2:2774819/1‑151 (MQ=255)
                                              gAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactaca                                                                                                     <  1:9369371/151‑1 (MQ=255)
                                                aaaTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaa                                                                                                   <  1:5160556/151‑1 (MQ=255)
                                                aaaTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaa                                                                                                   >  1:355102/1‑151 (MQ=255)
                                                 aaTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaac                                                                                                  <  2:4782991/151‑1 (MQ=255)
                                                       cgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgacta                                                                                            <  2:7893715/151‑1 (MQ=255)
                                                          cACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaa                                                                                         >  2:3950568/1‑151 (MQ=255)
                                                           aCTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaa                                                                                        <  2:10209816/151‑1 (MQ=255)
                                                             tCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaacc                                                                                      <  1:10667942/151‑1 (MQ=255)
                                                             tCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaacc                                                                                      <  1:9957278/151‑1 (MQ=255)
                                                              cGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATGTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGactacaaaccgactacaaaccg                                                                                     >  2:3758610/1‑151 (MQ=255)
                                                               ggCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACCAACCGACTACAAACCGt                                                                                    >  2:895984/1‑151 (MQ=255)
                                                                 cTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCt                                                                                  >  1:175773/1‑151 (MQ=255)
                                                                  tACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTGTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTa                                                                                 >  2:10478508/1‑151 (MQ=255)
                                                                   aCATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTAc                                                                                <  2:5639502/151‑1 (MQ=255)
                                                                   aCATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTAc                                                                                <  1:10092358/151‑1 (MQ=255)
                                                                   aCATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTAc                                                                                >  1:2429798/1‑151 (MQ=255)
                                                                     aTAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGc                                                                              >  1:5603774/1‑151 (MQ=255)
                                                                        gCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGcccc                                                                           >  1:7761621/1‑151 (MQ=255)
                                                                         cTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccccc                                                                          >  1:5982748/1‑151 (MQ=255)
                                                                         cTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCATTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccccc                                                                          <  2:8597079/151‑1 (MQ=255)
                                                                           cAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCtt                                                                        <  2:1123875/151‑1 (MQ=255)
                                                                           cAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCtt                                                                        <  1:659782/151‑1 (MQ=255)
                                                                             gTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTa                                                                      <  1:5314158/151‑1 (MQ=255)
                                                                                 gTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGa                                                                  >  2:7804921/1‑151 (MQ=255)
                                                                                    agagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAttt                                                               <  1:8779102/151‑1 (MQ=255)
                                                                                     gagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTa                                                              >  1:3355391/1‑151 (MQ=255)
                                                                                          cagcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGTTCGGCAGACTACAAACCGACTACATACCGACTACGCCCCCTTGACCGATTTACGGtt                                                         <  2:1259339/151‑1 (MQ=255)
                                                                                          cagcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGtt                                                         <  2:8276084/151‑1 (MQ=255)
                                                                                             caTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGc                                                      >  2:7529659/1‑151 (MQ=255)
                                                                                               ttCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCAt                                                    <  2:7252700/151‑1 (MQ=255)
                                                                                               atCATACTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCAt                                                    <  2:4698163/150‑1 (MQ=255)
                                                                                                tCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATg                                                   >  2:980162/1‑151 (MQ=255)
                                                                                                tCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATg                                                   >  2:5060919/1‑151 (MQ=255)
                                                                                                  aTAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaa                                                 >  1:8524501/1‑151 (MQ=255)
                                                                                                  aTAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaa                                                 <  1:3921990/151‑1 (MQ=255)
                                                                                                   tAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaa                                                >  1:7546085/1‑151 (MQ=255)
                                                                                                   tAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaa                                                >  1:4602592/1‑151 (MQ=255)
                                                                                                     aTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaaa                                              <  2:2138360/151‑1 (MQ=255)
                                                                                                     aTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaaa                                              >  2:6642853/1‑151 (MQ=255)
                                                                                                           aTGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCg                                        >  2:8117451/1‑151 (MQ=255)
                                                                                                              tCCCAGGTTCAAGTCCCTGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCAt                                     <  1:10187185/151‑1 (MQ=255)
                                                                                                               cccAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATc                                    >  1:8012198/1‑151 (MQ=255)
                                                                                                                ccAGGGTCAAGTCCCGGTGTAGCCACCATATATTTCAA‑GGGGTTAGCGCAAGCTATCACATTTTTGTTTTGGTAGGAAGACTACAAACAGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCt                                   <  2:10581672/151‑1 (MQ=255)
                                                                                                                 cAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTg                                  >  2:9114452/1‑151 (MQ=255)
                                                                                                                         aGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGctc                          <  1:1156391/151‑1 (MQ=255)
                                                                                                                         aGTCCCGGTGTAGCCACCAGATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCATTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTGACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGctc                          <  2:7078434/151‑1 (MQ=255)
                                                                                                                            cccGGTGTAGCCACCATAATTTTCCA‑GGGGTGAGCGCAAGCTAACCCCTATTTGTATTGGTCGGCAGACTACAACCCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTAc                       <  2:6515312/151‑1 (MQ=255)
                                                                                                                                gtgtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATc                   >  1:1901264/1‑151 (MQ=255)
                                                                                                                                 tgtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCa                  >  1:4761579/1‑151 (MQ=255)
                                                                                                                                  ttAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCaa                 <  1:2750706/150‑1 (MQ=255)
                                                                                                                                  gtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCTTCTGCGGGGCTCTACGATCaa                 >  1:1306774/1‑151 (MQ=255)
                                                                                                                                  gtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCaa                 >  1:6703128/1‑151 (MQ=255)
                                                                                                                                  gtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCaa                 <  1:10450588/151‑1 (MQ=255)
                                                                                                                                  gtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCaa                 <  2:5445384/151‑1 (MQ=255)
                                                                                                                                   tAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAAc                <  2:2929278/151‑1 (MQ=255)
                                                                                                                                    aGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACg               <  2:1399858/151‑1 (MQ=255)
                                                                                                                                     gCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGt              >  2:5366217/1‑151 (MQ=255)
                                                                                                                                       caccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTcc            >  1:1460757/1‑150 (MQ=255)
                                                                                                                                            tatTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCt       >  2:6207223/1‑151 (MQ=255)
                                                                                                                                              tttttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTAt     <  2:4076596/151‑1 (MQ=255)
                                                                                                                                              tttttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTAt     <  1:4294154/151‑1 (MQ=255)
                                                                                                                                                tttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATcc   <  1:3077231/151‑1 (MQ=255)
                                                                                                                                                tttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATcc   >  1:6929373/1‑151 (MQ=255)
                                                                                                                                                tttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATcc   <  1:10010572/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCCG  >  NC_008463/5513906‑5514202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑