BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,931,608	(T)_6→5	intergenic (‑27/‑151)	PA14_RS27130 ← / → PA14_RS27135	LysR family transcriptional regulator/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,931,603	0	T	.	100.0%	2323.9 / NA	471	intergenic (‑22/‑156)	PA14_RS27130/PA14_RS27135	LysR family transcriptional regulator/MFS transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (184/287); total (184/287)

Only 100 of 515 total aligned reads displayed.

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

cGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGgtgt                                                                                                                                                    <  1:1723609/151‑1 (MQ=255)

 gATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtt                                                                                                                                                   >  2:1534628/1‑151 (MQ=255)

 gATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtt                                                                                                                                                   >  1:3688953/1‑151 (MQ=255)

  aTTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttt                                                                                                                                                  <  2:74359/151‑1 (MQ=255)

  aTTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttt                                                                                                                                                  >  2:1906055/1‑151 (MQ=255)

   tttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttt                                                                                                                                                 <  1:1589918/151‑1 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGACGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGTTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttgt                                                                                                                                                >  2:7240813/1‑149 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttttt                                                                                                                                                <  2:9286671/151‑1 (MQ=255)

      cctcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttac                                                                                                                                              >  1:6768929/1‑149 (MQ=255)

      cctcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttac                                                                                                                                              <  1:7831533/151‑3 (MQ=255)

             cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                      <  1:7365603/151‑1 (MQ=255)

              aGCGCCTGTATCTGCTGGCTCAACGGCGGCTGGGAGATGCCCAGGGGCTCGGCGGCGCTGCCGACGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAAt                                                                                                                                     <  1:4263964/151‑1 (MQ=255)

              aGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAAt                                                                                                                                     <  1:3585665/151‑1 (MQ=255)

               gcgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATc                                                                                                                                    <  2:5807505/151‑1 (MQ=255)

               gcgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATc                                                                                                                                    <  1:3118668/151‑1 (MQ=255)

                cgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCg                                                                                                                                   >  2:7877770/1‑151 (MQ=255)

                 gcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCgt                                                                                                                                  <  2:688946/151‑1 (MQ=255)

                   cTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCgtgt                                                                                                                                <  2:3538684/151‑1 (MQ=255)

                    tGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTc                                                                                                                               <  2:8087895/151‑1 (MQ=255)

                      gATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGa                                                                                                                             <  1:931318/151‑1 (MQ=255)

                        tCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATc                                                                                                                           <  1:8255354/151‑1 (MQ=255)

                        tCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATc                                                                                                                           <  2:2034591/151‑1 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATCTCAATCGTGTCGATCAat                                                                                                                        >  2:8748390/1‑151 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        >  2:3847039/1‑151 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        <  1:10659486/151‑1 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        <  1:638227/151‑1 (MQ=255)

                             tgGCTCACCGGCGGCTGGGCGATCCCCAGGCGCTCTGCGGCGCGGACGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  1:1112460/151‑1 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCCATCGTGTCGATCAatat                                                                                                                      >  1:9520064/1‑151 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  2:9691955/151‑1 (MQ=255)

                                cTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATAtt                                                                                                                   <  2:10286944/151‑1 (MQ=255)

                                cTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATAtt                                                                                                                   <  2:6365444/151‑1 (MQ=255)

                                   aaCGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTCCATATCAATCGTGTCGATCAATATATTGGa                                                                                                                <  2:10751013/151‑1 (MQ=255)

                                   aaCGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGa                                                                                                                >  2:10114608/1‑151 (MQ=255)

                                        cggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCa                                                                                                           >  2:8243037/1‑151 (MQ=255)

                                        cggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGAGCAATATATTGGAAATCa                                                                                                           >  2:4032642/1‑151 (MQ=255)

                                          gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCata                                                                                                         <  1:9044027/151‑1 (MQ=255)

                                          gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCata                                                                                                         <  1:8033132/151‑1 (MQ=255)

                                                agaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAAcc                                                                                                   <  1:5571011/151‑1 (MQ=255)

                                                  aTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAACTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACcta                                                                                                 <  2:4598656/151‑1 (MQ=255)

                                                     cccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                              <  1:3396901/151‑1 (MQ=255)

                                                     cccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                              <  1:1957813/151‑1 (MQ=255)

                                                      ccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatcta                                                                                             >  1:2870549/1‑151 (MQ=255)

                                                       cAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatctat                                                                                            <  1:4230654/151‑1 (MQ=255)

                                                       cAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatctat                                                                                            >  1:6255569/1‑151 (MQ=255)

                                                         ggCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCTATATATTGGAAATCATATAAACCTATCTatat                                                                                          <  1:8878767/151‑1 (MQ=255)

                                                          gcgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATc                                                                                         <  1:6428435/151‑1 (MQ=255)

                                                           cgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATcc                                                                                        <  1:8380463/151‑1 (MQ=255)

                                                             cTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCtt                                                                                      <  1:1491860/151‑1 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     >  2:2225148/1‑151 (MQ=255)

                                                                ggcggcGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGc                                                                                   <  1:472486/151‑1 (MQ=255)

                                                                 gcggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATCTCAATCGTGTCGATCAATATATTGGAAATCATATTAACCTATCTATATCCTTTGcc                                                                                  <  2:1663218/151‑1 (MQ=255)

                                                                 gcggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGcc                                                                                  <  2:7014256/151‑1 (MQ=255)

                                                                   ggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCcac                                                                                <  1:5797294/151‑1 (MQ=255)

                                                                    gcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGCAAATCATATAAACCTATCTATATCCTTTGCcaca                                                                               <  1:5420941/151‑1 (MQ=255)

                                                                      gcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATa                                                                             >  1:7506949/1‑151 (MQ=255)

                                                                      gcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATa                                                                             <  1:9677007/151‑1 (MQ=255)

                                                                       cggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATaa                                                                            <  1:2303012/151‑1 (MQ=255)

                                                                        ggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                           >  1:7090116/1‑151 (MQ=255)

                                                                            gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTcc                                                                       <  2:8898581/151‑1 (MQ=255)

                                                                             aaGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCca                                                                      >  1:6924189/1‑151 (MQ=255)

                                                                             aaGTGCAGCTCCGCGGCGACGGCGAGGAAAGAGCGCAGGGGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATCTCAAGCGTGTCGATCAATCTATTGGAAATCATATAAACCTAGCTATATCCTTTGCCCCATAACCTCca                                                                      <  2:8350973/151‑1 (MQ=255)

                                                                              aGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaa                                                                     <  2:5072361/151‑1 (MQ=255)

                                                                                tGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaaca                                                                   <  2:6011997/151‑1 (MQ=255)

                                                                                tGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaaca                                                                   <  1:4909361/151‑1 (MQ=255)

                                                                                  cAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCaacaac                                                                 <  2:7653841/151‑1 (MQ=255)

                                                                                      tcctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAcc                                                             <  1:102504/151‑1 (MQ=255)

                                                                                              gacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTcc                                                     >  2:4260809/1‑151 (MQ=255)

                                                                                               acggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTccc                                                    >  2:1223698/1‑151 (MQ=255)

                                                                                                cggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCt                                                   >  2:2356573/1‑151 (MQ=255)

                                                                                                 ggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTg                                                  <  1:9118662/151‑1 (MQ=255)

                                                                                                    gaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGaaa                                               >  1:8755213/1‑151 (MQ=255)

                                                                                                     aTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAAt                                              <  2:2578652/151‑1 (MQ=255)

                                                                                                       gAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATcc                                            >  2:3326914/1‑151 (MQ=255)

                                                                                                             gcgcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTgg                                      <  1:5092049/151‑1 (MQ=255)

                                                                                                               gcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcg                                    >  1:10173349/1‑151 (MQ=255)

                                                                                                               gcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcg                                    <  2:1690507/151‑1 (MQ=255)

                                                                                                               gcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATAACAATCGTGTCGATCAATATATTGGAAATCATATAAAACTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcg                                    <  1:10447704/151‑1 (MQ=255)

                                                                                                                 aGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGcgcg                                  <  1:1300955/151‑1 (MQ=255)

                                                                                                                 aGGTGCCTGAGGTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCATCAACGACCCCTTGTCCCTGAAATCCAGGTGGcgcg                                  >  2:5424052/1‑151 (MQ=255)

                                                                                                                    tGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGcc                               >  1:989906/1‑151 (MQ=255)

                                                                                                                    tGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGcc                               <  1:4086906/151‑1 (MQ=255)

                                                                                                                        tGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGt                           <  2:3028458/151‑1 (MQ=255)

                                                                                                                        tGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGt                           <  1:2534633/151‑1 (MQ=255)

                                                                                                                          cgTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TATTTACATATCAATCGTGTCGATCAATATATTGGACATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtg                         <  1:3825582/150‑1 (MQ=255)

                                                                                                                             tCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATACACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGc                      >  1:6067633/1‑151 (MQ=255)

                                                                                                                             tCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGc                      >  1:10011682/1‑151 (MQ=255)

                                                                                                                                  gCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCt                 <  2:6688109/151‑1 (MQ=255)

                                                                                                                                  gCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCt                 <  2:4392843/151‑1 (MQ=255)

                                                                                                                                   cGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAATCCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTc                >  2:2900102/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  1:2132515/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  1:3027571/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  1:4202367/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  1:8469081/1‑151 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               >  1:8599690/1‑151 (MQ=255)

                                                                                                                                       cccTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCa            >  1:4539708/1‑151 (MQ=255)

                                                                                                                                          tcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCg         >  2:8624568/1‑151 (MQ=255)

                                                                                                                                            cctattAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGt        >  2:10603690/5‑151 (MQ=255)

                                                                                                                                               attAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTccc     <  2:9544927/150‑1 (MQ=255)

                                                                                                                                               attAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTccc     <  2:9788844/150‑1 (MQ=255)

                                                                                                                                                  aGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCag  >  2:4751307/1‑151 (MQ=255)

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`