BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,239,163	(C)_5→4	intergenic (+161/‑32)	PA14_RS05795 → / → lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,239,159	0	C	.	100.0%	2305.5 / NA	463	intergenic (+157/‑36)	PA14_RS05795/lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (236/227); total (236/227)

Only 100 of 484 total aligned reads displayed.

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCAA  >  NC_008463/1239010‑1239305

     tGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCCTGGCCGGACACGCGTGATGACGGGGCCGACGGGccccgtc                                                                                                                                              >  2:5625439/1‑148 (MQ=255)

       aaCGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCag                                                                                                                                           >  1:485844/1‑151 (MQ=255)

        aCGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCaga                                                                                                                                          >  2:2012444/1‑151 (MQ=255)

          gAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGc                                                                                                                                        >  2:7447715/1‑151 (MQ=255)

           aGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGcc                                                                                                                                       <  1:41835/151‑1 (MQ=255)

            gCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCt                                                                                                                                      >  2:9860171/1‑151 (MQ=255)

              gTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                    >  1:1244056/1‑151 (MQ=255)

                tCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGACTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCa                                                                                                                                  >  2:5007098/1‑151 (MQ=255)

                 cGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCaa                                                                                                                                 >  1:8939173/1‑151 (MQ=255)

                  gCCTCGACGGTCTCTCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCAAGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCGGGCAAc                                                                                                                                >  2:5940190/1‑151 (MQ=255)

                  gCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                                >  2:10577441/1‑151 (MQ=255)

                  gCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                                >  1:8185059/1‑151 (MQ=255)

                     tCGACGGTCTATCGGCCTCGTGGGACGGCCAGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCAGGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCa                                                                                                                             <  1:2764050/151‑1 (MQ=255)

                      cGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAg                                                                                                                            >  1:6831703/1‑151 (MQ=255)

                       gACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAgg                                                                                                                           >  1:4343055/1‑151 (MQ=255)

                        aCGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGACTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCAGGGCCGGAAACGCGTGCTGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGa                                                                                                                          <  2:761929/151‑1 (MQ=255)

                        aCGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGa                                                                                                                          <  1:10210122/151‑1 (MQ=255)

                         cGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAg                                                                                                                         <  1:381562/151‑1 (MQ=255)

                              tATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                    >  1:8802614/1‑151 (MQ=255)

                                 cGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGa                                                                                                                 <  2:8784167/151‑1 (MQ=255)

                                  gggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAt                                                                                                                >  2:428949/1‑151 (MQ=255)

                                   ggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAtt                                                                                                               <  1:10469739/151‑1 (MQ=255)

                                     ctctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGa                                                                                                             <  2:6583073/151‑1 (MQ=255)

                                      tctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTCTTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGAt                                                                                                            >  2:1801966/1‑151 (MQ=255)

                                        ttGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCg                                                                                                          <  1:1514698/151‑1 (MQ=255)

                                         tGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGt                                                                                                         >  1:3582097/1‑151 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGt                                                                                                  >  2:2794390/1‑151 (MQ=255)

                                                  cTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTa                                                                                                >  1:4627361/1‑151 (MQ=255)

                                                  cTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTa                                                                                                >  1:235855/1‑151 (MQ=255)

                                                   tGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAt                                                                                               >  2:258245/1‑151 (MQ=255)

                                                    gcgcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAtt                                                                                              <  2:8314743/151‑1 (MQ=255)

                                                        aGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTc                                                                                          >  1:1364411/1‑151 (MQ=255)

                                                          tGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTccc                                                                                        >  2:5747896/1‑151 (MQ=255)

                                                           gACAGGCCCGCCGACTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCg                                                                                       <  2:7110963/151‑1 (MQ=255)

                                                           gACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGNGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCg                                                                                       <  1:5648703/151‑1 (MQ=255)

                                                            aCAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGt                                                                                      >  2:1137889/1‑151 (MQ=255)

                                                             cAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTg                                                                                     <  1:3177230/151‑1 (MQ=255)

                                                               ggCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGaa                                                                                   >  1:7239767/1‑151 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                 <  1:6391251/151‑1 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                 <  1:2963084/151‑1 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                 <  2:3625843/151‑1 (MQ=255)

                                                                  ccgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTg                                                                                >  1:3323880/1‑151 (MQ=255)

                                                                  ccgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTg                                                                                <  1:1977649/151‑1 (MQ=255)

                                                                   cgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGc                                                                               <  1:9899062/151‑1 (MQ=255)

                                                                    gccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCt                                                                              <  2:3776976/151‑1 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTCTTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTggg                                                                           >  2:9414247/1‑150 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGt                                                                           >  1:9410910/1‑151 (MQ=255)

                                                                        aaTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGGGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTc                                                                          >  1:4990484/1‑151 (MQ=255)

                                                                          tCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAc                                                                        >  2:9129810/1‑151 (MQ=255)

                                                                          tCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAc                                                                        <  1:10661529/151‑1 (MQ=255)

                                                                            gCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                      >  2:1597333/1‑151 (MQ=255)

                                                                                gATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTGTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAg                                                                  >  2:5182700/1‑151 (MQ=255)

                                                                                  tAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcg                                                                >  2:9582831/1‑151 (MQ=255)

                                                                                   aaTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcgc                                                               >  2:8469479/1‑151 (MQ=255)

                                                                                   aaTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcgc                                                               >  1:9991844/1‑151 (MQ=255)

                                                                                    aTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATGTGTCCCGTGAAGTGCTGGTCACCATGAGCGcc                                                              >  2:67888/1‑151 (MQ=255)

                                                                                     tGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCg                                                             <  2:10359742/151‑1 (MQ=255)

                                                                                      gCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGt                                                            <  2:9711894/151‑1 (MQ=255)

                                                                                      gCCGGCTATTTTCACCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGt                                                            >  2:7773659/1‑151 (MQ=255)

                                                                                       ccGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTc                                                           >  1:6528672/1‑151 (MQ=255)

                                                                                       ccGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTc                                                           <  1:584377/151‑1 (MQ=255)

                                                                                          gCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgc                                                        <  1:1003104/151‑1 (MQ=255)

                                                                                           cTATGTTCTCCGCGGCATTGCGCCAGGGCCGGAAACGCGTGATGAAGGGGCCGACGGG‑CACCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTCTTTGTCACGTTAAGTGCTGTGCACCATGAGCGCCGTCAgcg                                                       >  2:4216430/1‑151 (MQ=255)

                                                                                            tATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcgc                                                      >  1:7965851/1‑151 (MQ=255)

                                                                                               tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGt                                                   <  1:7304106/151‑1 (MQ=255)

                                                                                                ttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtg                                                  >  2:6345586/1‑151 (MQ=255)

                                                                                                  ctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgct                                                >  2:3207524/1‑151 (MQ=255)

                                                                                                  ctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgct                                                <  1:1838847/151‑1 (MQ=255)

                                                                                                   tcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCAGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgctg                                               >  2:221472/1‑151 (MQ=255)

                                                                                                        gcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGACGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGt                                          >  2:6501594/1‑151 (MQ=255)

                                                                                                          cATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGa                                        <  1:5766535/151‑1 (MQ=255)

                                                                                                          cATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCGGGTGa                                        >  2:2327520/1‑151 (MQ=255)

                                                                                                           aTGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                       >  1:9011883/1‑151 (MQ=255)

                                                                                                            tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatc                                      <  2:7388953/151‑1 (MQ=255)

                                                                                                              ccgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcat                                    <  1:9569390/151‑1 (MQ=255)

                                                                                                              ccgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcat                                    <  1:6080990/151‑1 (MQ=255)

                                                                                                               cgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcatc                                   >  1:8424127/1‑151 (MQ=255)

                                                                                                                gccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                  >  1:5417452/1‑151 (MQ=255)

                                                                                                                gccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                  >  2:4614955/1‑151 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATcatcat                                 <  1:7722843/151‑1 (MQ=255)

                                                                                                                   aTGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGa                               >  1:2130067/1‑151 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                             <  1:6184207/151‑1 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                             >  2:1297381/1‑151 (MQ=255)

                                                                                                                           aaaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGt                       <  2:3419377/151‑1 (MQ=255)

                                                                                                                           aaaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGt                       >  2:8077026/1‑151 (MQ=255)

                                                                                                                             aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGttt                     >  2:7020399/1‑151 (MQ=255)

                                                                                                                              cgcgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtc                    <  2:1958906/151‑1 (MQ=255)

                                                                                                                                cgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtcat                  <  1:7041205/151‑1 (MQ=255)

                                                                                                                                   gatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCaa               <  1:3082746/151‑1 (MQ=255)

                                                                                                                                   gatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATGTGTCCCGTGAAGTGCGGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCaa               <  2:621695/151‑1 (MQ=255)

                                                                                                                                      gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTa            >  2:3026139/1‑151 (MQ=255)

                                                                                                                                        cGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc          >  1:5100404/1‑151 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt         <  2:8628731/151‑1 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt         <  2:4012523/151‑1 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt         >  1:4002178/1‑151 (MQ=255)

                                                                                                                                           ggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTgg       <  2:5382563/151‑1 (MQ=255)

                                                                                                                                            gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgc      <  1:1698015/151‑1 (MQ=255)

                                                                                                                                             ccGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgcg     <  1:9269262/151‑1 (MQ=255)

                                                                                                                                             ccGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgcg     <  2:5513182/151‑1 (MQ=255)

                                                                                                                                               gACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCa   >  1:4506020/1‑151 (MQ=255)

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCAA  >  NC_008463/1239010‑1239305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`