Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,543,6620AG100.0% 2287.3 / NA 572T494A (ACG→GCG) PA14_RS16110PucR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (252/320);  total (252/320)

Only 100 of 572 total aligned reads displayed.

GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCG  >  NC_008463/3543514‑3543808
                                                                                                                                                    |                                                                                                                                                  
gAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCTGGCAACCCGGCTGTCGCCGGAAGCg                                                                                                                                                  >  1:4037586/1‑151 (MQ=255)
gAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCg                                                                                                                                                  >  1:7593592/1‑151 (MQ=255)
  aCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAg                                                                                                                                                <  1:5690655/151‑1 (MQ=255)
   cgccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgg                                                                                                                                               <  2:10005748/151‑1 (MQ=255)
   cgccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgg                                                                                                                                               >  1:7890127/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                            >  2:9703316/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                            >  2:2842118/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCCCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                            >  1:8764011/1‑151 (MQ=255)
        cgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAg                                                                                                                                          >  2:7278718/1‑151 (MQ=255)
         gcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                         >  1:1896843/1‑151 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                       <  2:10204642/151‑1 (MQ=255)
            gCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACg                                                                                                                                      >  1:6772472/1‑151 (MQ=255)
              ggCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGc                                                                                                                                    >  1:892861/1‑151 (MQ=255)
                cTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGa                                                                                                                                  >  1:3252724/1‑151 (MQ=255)
                      atacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                            <  2:3198851/151‑1 (MQ=255)
                        acacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagcagc                                                                                                                          <  1:5520541/151‑1 (MQ=255)
                         cacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcc                                                                                                                         >  1:940362/1‑151 (MQ=255)
                          acaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcct                                                                                                                        <  1:3640838/151‑1 (MQ=255)
                           caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctc                                                                                                                       <  2:6776142/151‑1 (MQ=255)
                             aGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                     <  2:1827511/151‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                  <  2:5361913/151‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                  <  1:1504865/151‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                  <  1:6978618/151‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                  >  1:206757/1‑151 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                  >  2:9147776/1‑151 (MQ=255)
                                 gagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAg                                                                                                                 >  2:6365071/1‑151 (MQ=255)
                                   gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               >  2:3747309/1‑151 (MQ=255)
                                   gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                               <  2:2453847/151‑1 (MQ=255)
                                     cTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGt                                                                                                             <  1:5406446/151‑1 (MQ=255)
                                      tGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTc                                                                                                            <  2:1603428/151‑1 (MQ=255)
                                         tCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgc                                                                                                         <  2:101411/151‑1 (MQ=255)
                                         tCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgc                                                                                                         >  1:10558423/1‑151 (MQ=255)
                                         tCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgc                                                                                                         >  1:7148870/1‑151 (MQ=255)
                                           tACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCgcgc                                                                                                       >  2:2192555/1‑151 (MQ=255)
                                           tACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCgcgc                                                                                                       <  2:5059963/151‑1 (MQ=255)
                                           gaCCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCgcgc                                                                                                       >  1:9354912/2‑151 (MQ=255)
                                             ccGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAc                                                                                                     >  2:6450211/1‑151 (MQ=255)
                                              cGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAcc                                                                                                    >  1:1152588/1‑151 (MQ=255)
                                                   aCGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggc                                                                                               <  1:10733949/151‑1 (MQ=255)
                                                    cgccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGATGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcg                                                                                              >  1:6514690/1‑151 (MQ=255)
                                                    cgccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcg                                                                                              <  2:2306906/151‑1 (MQ=255)
                                                    cgccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcg                                                                                              <  2:2678543/151‑1 (MQ=255)
                                                       cgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGACTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGgcggcg                                                                                           <  1:1684419/151‑1 (MQ=255)
                                                         cATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGAc                                                                                         <  1:4974538/151‑1 (MQ=255)
                                                          aTCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACa                                                                                        >  2:7007802/1‑151 (MQ=255)
                                                              aGGAAATCAGCGGTCGCTCGCTGGACCGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGa                                                                                    <  2:4516853/151‑1 (MQ=255)
                                                               ggAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGAc                                                                                   <  1:5907220/151‑1 (MQ=255)
                                                                gAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACt                                                                                  <  1:7788358/151‑1 (MQ=255)
                                                                  aaTAAGCGGACGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGATGGGCAGTAAGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACAGa                                                                                >  1:9757728/1‑151 (MQ=255)
                                                                     cAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcgg                                                                             <  1:5132497/151‑1 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                         >  2:1470450/1‑151 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                         >  2:3632002/1‑151 (MQ=255)
                                                                            cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                      <  1:2253253/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                     >  2:3908798/1‑151 (MQ=255)
                                                                              ctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCg                                                                    >  1:10040356/1‑151 (MQ=255)
                                                                              ctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCAAGGCGGCGACATGGACTGACGGCGGAGCCCg                                                                    <  2:3378137/151‑1 (MQ=255)
                                                                               tcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGa                                                                   <  1:6065439/151‑1 (MQ=255)
                                                                               tcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGa                                                                   <  2:6623350/151‑1 (MQ=255)
                                                                                 gctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATa                                                                 >  1:2958713/1‑151 (MQ=255)
                                                                                 gctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATa                                                                 <  1:383867/151‑1 (MQ=255)
                                                                                    ggACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCt                                                              <  2:1318962/151‑1 (MQ=255)
                                                                                     gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTg                                                             >  2:5758570/1‑151 (MQ=255)
                                                                                     gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTg                                                             <  2:7477976/151‑1 (MQ=255)
                                                                                     gACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTg                                                             <  1:7355378/151‑1 (MQ=255)
                                                                                      aCAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTgg                                                            <  2:1067805/151‑1 (MQ=255)
                                                                                       cAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGnc                                                           <  1:8535822/151‑1 (MQ=255)
                                                                                        aGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGTCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                          <  2:5219911/151‑1 (MQ=255)
                                                                                        aGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                          >  2:4117484/1‑151 (MQ=255)
                                                                                          cACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCt                                                        <  2:984564/151‑1 (MQ=255)
                                                                                             cgccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGc                                                     >  2:3478812/1‑151 (MQ=255)
                                                                                                cgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAgg                                                  <  1:9201769/151‑1 (MQ=255)
                                                                                                 gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGga                                                 <  2:4680795/151‑1 (MQ=255)
                                                                                                 gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGga                                                 <  1:814362/151‑1 (MQ=255)
                                                                                                 gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGga                                                 >  2:5510858/1‑151 (MQ=255)
                                                                                                    gtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacga                                              >  1:2896120/1‑151 (MQ=255)
                                                                                                        cGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgcc                                          <  2:8473024/151‑1 (MQ=255)
                                                                                                        cGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgcc                                          >  1:669853/1‑151 (MQ=255)
                                                                                                          aCCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgc                                        <  2:5906719/151‑1 (MQ=255)
                                                                                                           cccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                       <  2:9487863/151‑1 (MQ=255)
                                                                                                              tctGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACgccgccgcc                                    >  1:10144682/1‑151 (MQ=255)
                                                                                                              tctGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACgccgccgcc                                    >  2:8967120/1‑151 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                 >  2:1990707/1‑151 (MQ=255)
                                                                                                                   ttCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAg                               <  1:4653191/151‑1 (MQ=255)
                                                                                                                    tCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAgg                              <  1:2540053/151‑1 (MQ=255)
                                                                                                                     cGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgc                             <  1:8367463/151‑1 (MQ=255)
                                                                                                                       ccTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcgc                           <  2:4296472/151‑1 (MQ=255)
                                                                                                                             aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                     >  2:5649189/1‑151 (MQ=255)
                                                                                                                               gCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGa                   <  1:524275/151‑1 (MQ=255)
                                                                                                                                cAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGaa                  <  1:4245526/151‑1 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                 <  2:8185300/151‑1 (MQ=255)
                                                                                                                                  ccccGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                <  2:9414633/150‑1 (MQ=255)
                                                                                                                                  aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                <  1:6347894/151‑1 (MQ=255)
                                                                                                                                  aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                <  2:8619188/151‑1 (MQ=255)
                                                                                                                                  aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                >  2:7584703/1‑151 (MQ=255)
                                                                                                                                     cGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGtt             >  1:3379103/1‑151 (MQ=255)
                                                                                                                                       gCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGc           >  2:3275983/1‑151 (MQ=255)
                                                                                                                                         tGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCt         >  2:2577926/1‑151 (MQ=255)
                                                                                                                                          gTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTg        >  1:4452653/1‑151 (MQ=255)
                                                                                                                                               cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgc   >  1:2707644/1‑151 (MQ=255)
                                                                                                                                                ggAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgcg  >  2:7751333/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCG  >  NC_008463/3543514‑3543808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑