Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,769,180 C→G A44A (GCG→GCC)  pscL ← SctL family type III secretion system stator protein PscL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,769,1800CG99.8% 2015.9 / NA 511A44A (GCG→GCC) pscLSctL family type III secretion system stator protein PscL
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (264/245);  minor base T (1/0);  total (266/245)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 523 total aligned reads displayed.

GGCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTC  >  NC_008463/3769032‑3769327
                                                                                                                                                    |                                                                                                                                                   
   gATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcggc                                                                                                                                                <  1:4892039/151‑1 (MQ=255)
    aTCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTTGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCATCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCa                                                                                                                                               >  2:1435869/1‑151 (MQ=255)
       gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                            >  1:1594771/1‑151 (MQ=255)
       gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                            >  1:4517954/1‑151 (MQ=255)
       gACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                            <  1:2485063/151‑1 (MQ=255)
        aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCt                                                                                                                                           <  1:1226103/151‑1 (MQ=255)
         ccTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                          >  1:1812251/1‑151 (MQ=255)
            gCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgcg                                                                                                                                       >  2:2360802/1‑151 (MQ=255)
            gCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgcg                                                                                                                                       >  2:2013990/1‑151 (MQ=255)
               ggTAGTAGCGATTGCAGCGCAGTAGCGTGTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGc                                                                                                                                    <  2:4539149/151‑1 (MQ=255)
                gtagtaGCGAGTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGcc                                                                                                                                   <  2:9948172/151‑1 (MQ=255)
                  agtagCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGc                                                                                                                                 <  1:10167349/151‑1 (MQ=255)
                    tagCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCtt                                                                                                                               >  1:1437167/1‑151 (MQ=255)
                      gCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTcc                                                                                                                             >  2:6159200/1‑151 (MQ=255)
                        gATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGAACGCTCGCGTGCCGCTTccac                                                                                                                           <  2:3850755/151‑1 (MQ=255)
                         aTTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTccacc                                                                                                                          <  1:10637420/151‑1 (MQ=255)
                             cAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGc                                                                                                                      >  1:3038261/1‑151 (MQ=255)
                             cAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGc                                                                                                                      <  1:4236019/151‑1 (MQ=255)
                              tgcgcTGTTGGGTTTCGTGTATCAGCCGGGCCTGGCGCAGACGCGCCTCCTCATGTCGGGCCTCCCCGCCCTGGGGATTGTGGTCGTGTTAAACCTGGTGGGCCTGGCGCTCGTTTTGGGGGGCAGGCTGGTGGGCCGCTACCACCAGGCg                                                                                                                     <  2:2236436/150‑1 (MQ=255)
                               gcgcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCgg                                                                                                                    <  2:9175447/151‑1 (MQ=255)
                                cgcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGt                                                                                                                   >  2:867562/1‑151 (MQ=255)
                                cgcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGt                                                                                                                   >  1:4407255/1‑151 (MQ=255)
                                 gcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAAACTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGtt                                                                                                                  >  2:5343251/1‑151 (MQ=255)
                                  cAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTg                                                                                                                 <  1:10670565/151‑1 (MQ=255)
                                   aGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTgg                                                                                                                >  1:8065932/1‑151 (MQ=255)
                                    gTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgc                                                                                                               >  2:7929043/1‑151 (MQ=255)
                                     tAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcg                                                                                                              <  2:4991269/151‑1 (MQ=255)
                                      aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCTGGCGGTTGgcgg                                                                                                             >  2:6577523/1‑150 (MQ=255)
                                         gTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGa                                                                                                          >  1:2213647/1‑151 (MQ=255)
                                         gTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGa                                                                                                          <  1:5876737/151‑1 (MQ=255)
                                         gTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGa                                                                                                          >  2:5879629/1‑151 (MQ=255)
                                           ttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGATCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCTCGTGCCGCATCCACCAGGCGGTTGGCGCTGAgg                                                                                                        >  2:10458798/1‑151 (MQ=255)
                                           ttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTGCTCGTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATGTCCGCGGCTCGCGCGCGTGCCGCTTCCACCAGGCGGTTGGGGCTGAgg                                                                                                        >  1:6685932/1‑151 (MQ=255)
                                            tCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCCGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGt                                                                                                       <  2:354127/151‑1 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGtg                                                                                                      >  1:3857088/1‑150 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGtc                                                                                                      >  1:9765109/1‑150 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                      >  1:10534575/1‑151 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                      >  2:8473660/1‑151 (MQ=255)
                                              cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                     <  2:1626791/151‑1 (MQ=255)
                                              cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                     >  2:10277650/1‑151 (MQ=255)
                                              cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGGGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                     >  1:6319/1‑151 (MQ=255)
                                                   tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGGTGGCGCTGAGGTAGTCCTg                                                                                                >  2:4935223/1‑151 (MQ=255)
                                                    cAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                               >  1:6443116/1‑151 (MQ=255)
                                                       cccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGt                                                                                                       >  1:6859027/1‑140 (MQ=255)
                                                       cccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAg                                                                                            <  1:6153448/151‑1 (MQ=255)
                                                         cGGCCTGGCGCAGACGCGCCTCCTCCAGTACGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCGCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                          >  2:298573/1‑151 (MQ=255)
                                                          ggCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTACGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcg                                                                                         >  2:9788105/1‑151 (MQ=255)
                                                            ccTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcgcg                                                                                       <  1:2606042/151‑1 (MQ=255)
                                                              tGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                     >  1:3122041/1‑151 (MQ=255)
                                                              tGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                     >  1:4979118/1‑151 (MQ=255)
                                                                    aGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCa                                                                               >  2:3372109/1‑151 (MQ=255)
                                                                    aGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCa                                                                               >  1:8606576/1‑151 (MQ=255)
                                                                        gcgcCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAg                                                                           >  1:2877199/1‑151 (MQ=255)
                                                                            ctcctcCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                       <  1:2004372/151‑1 (MQ=255)
                                                                            ctcctcCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                       <  2:8311528/151‑1 (MQ=255)
                                                                             tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCt                                                                      <  1:9149187/151‑1 (MQ=255)
                                                                              cctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCCCGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCACCAGGGCCTg                                                                     >  1:8980902/1‑151 (MQ=255)
                                                                                 ccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGccc                                                                  <  2:7201342/151‑1 (MQ=255)
                                                                                  cAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTACACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTCGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCg                                                                 >  1:4756808/1‑151 (MQ=255)
                                                                                   aGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                                <  2:8959911/151‑1 (MQ=255)
                                                                                      ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggggg                                                             >  1:9981524/1‑151 (MQ=255)
                                                                                      ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggggg                                                             >  2:6450964/1‑151 (MQ=255)
                                                                                       cGGCCTCCCAGCCCAGGCGCTTCGGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGc                                                            <  1:5326680/151‑1 (MQ=255)
                                                                                         gCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCa                                                          <  2:5985648/151‑1 (MQ=255)
                                                                                             cccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCg                                                      <  1:3220839/151‑1 (MQ=255)
                                                                                              ccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGt                                                     >  1:732122/1‑151 (MQ=255)
                                                                                              ccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGt                                                     <  1:7586658/151‑1 (MQ=255)
                                                                                                 gcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCCACAGGGCCTGCCCGGGGGCCAGCCGTAcg                                                  >  1:3769624/1‑151 (MQ=255)
                                                                                                  cccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                 >  2:8063453/1‑151 (MQ=255)
                                                                                                  cccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGGTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgc                                                 >  2:7604816/1‑151 (MQ=255)
                                                                                                   ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgcg                                                >  2:8181146/1‑151 (MQ=255)
                                                                                                         gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGc                                          >  2:6753860/1‑151 (MQ=255)
                                                                                                          cTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCg                                         <  1:8193691/151‑1 (MQ=255)
                                                                                                             cTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCt                                      <  2:7377511/151‑1 (MQ=255)
                                                                                                              tGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                     >  1:9549233/1‑151 (MQ=255)
                                                                                                               gCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAg                                    <  1:9848684/151‑1 (MQ=255)
                                                                                                                cTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGt                                   <  1:7148882/151‑1 (MQ=255)
                                                                                                                 tCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGtt                                  <  1:9345241/151‑1 (MQ=255)
                                                                                                                  ccTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTc                                 <  1:7035222/151‑1 (MQ=255)
                                                                                                                   cTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTca                                <  1:3372832/151‑1 (MQ=255)
                                                                                                                       gaaaCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaacaa                            <  2:9432191/150‑1 (MQ=255)
                                                                                                                        aaaCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACaaa                           >  2:4803094/1‑151 (MQ=255)
                                                                                                                         aaCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAAt                          <  2:3117606/151‑1 (MQ=255)
                                                                                                                           ccTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATgg                        >  2:9857396/1‑151 (MQ=255)
                                                                                                                            cTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                       <  2:4259748/151‑1 (MQ=255)
                                                                                                                               gTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                    <  2:5561232/151‑1 (MQ=255)
                                                                                                                               gTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                    <  1:10292809/151‑1 (MQ=255)
                                                                                                                               gTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                    >  2:7669588/1‑151 (MQ=255)
                                                                                                                                tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCa                   >  2:114332/1‑151 (MQ=255)
                                                                                                                                 gggCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCAt                  <  1:2729275/151‑1 (MQ=255)
                                                                                                                                  ggCCTCGCGCTCGTTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCAtt                 <  2:9654610/151‑1 (MQ=255)
                                                                                                                                    ccTCGCGATCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCa               <  1:10024116/151‑1 (MQ=255)
                                                                                                                                     cTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAt              <  1:7860501/151‑1 (MQ=255)
                                                                                                                                      tCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtc             <  2:4672992/151‑1 (MQ=255)
                                                                                                                                       cgcgCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcg            <  2:2706766/151‑1 (MQ=255)
                                                                                                                                           cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcg        <  2:4053788/151‑1 (MQ=255)
                                                                                                                                            tCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcgc       >  1:5613774/1‑151 (MQ=255)
                                                                                                                                             cGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATcgctcgct      <  2:1174468/151‑1 (MQ=255)
                                                                                                                                             cGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATcgctcgct      <  2:686222/151‑1 (MQ=255)
                                                                                                                                              gATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt     <  2:1009167/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTC  >  NC_008463/3769032‑3769327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑