Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,787 G→C A208A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,7870GC99.8% 2290.7 / NA 585A208A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (273/311);  minor base A (0/1);  total (273/312)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 611 total aligned reads displayed.

TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934
                                                                                                                                                    |                                                                                                                                                   
 ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAg                                                                                                                                                  <  1:4004826/151‑1 (MQ=255)
     aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa                                                                                                                                              <  2:5340435/151‑1 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCTTCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          <  2:1268033/151‑1 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          >  2:9922944/1‑151 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          <  1:6180921/151‑1 (MQ=255)
          ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc                                                                                                                                         >  1:1326107/1‑151 (MQ=255)
          ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc                                                                                                                                         <  1:9256345/151‑1 (MQ=255)
              ggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATa                                                                                                                                     >  2:3346001/1‑151 (MQ=255)
                 gccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                  >  1:10697945/4‑151 (MQ=255)
                   cgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAcc                                                                                                                                <  2:520140/150‑1 (MQ=255)
                   agAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACATGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAcc                                                                                                                                >  2:2321452/2‑151 (MQ=255)
                    gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCg                                                                                                                               <  1:413507/151‑1 (MQ=255)
                       gCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                            <  2:2054382/151‑1 (MQ=255)
                        cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                           >  1:7015195/1‑151 (MQ=255)
                          aTCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCt                                                                                                                         <  2:6538457/151‑1 (MQ=255)
                           tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTtgtg                                                                                                                        <  1:3159776/151‑1 (MQ=255)
                           acGGCGCGGTGCGGCGCGACGAGCACCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGCTGGAAGGGATCTTCCCCGGCGGCGCCGAGGTGATCTTCGATACGACCGGTTTCTg                                                                                                                        <  2:10286974/150‑1 (MQ=255)
                            cGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTgg                                                                                                                       <  1:4389500/151‑1 (MQ=255)
                            cGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTgg                                                                                                                       >  1:2977557/1‑151 (MQ=255)
                             ggCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGc                                                                                                                      >  1:8143714/1‑151 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                 >  2:845677/1‑151 (MQ=255)
                                   gTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                                <  1:10490641/151‑1 (MQ=255)
                                   gTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                                >  2:1426998/1‑151 (MQ=255)
                                     gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcg                                                                                                              <  2:1789520/151‑1 (MQ=255)
                                      cggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcgg                                                                                                             >  2:1933934/1‑151 (MQ=255)
                                         cgcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGcggcgg                                                                                                          >  2:7008599/1‑151 (MQ=255)
                                          gcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGt                                                                                                         >  1:4953606/1‑151 (MQ=255)
                                             aggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTccc                                                                                                      >  1:231031/1‑151 (MQ=255)
                                              ggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCg                                                                                                     <  1:6010393/151‑1 (MQ=255)
                                                agCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgc                                                                                                   <  1:8976484/151‑1 (MQ=255)
                                                 gCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcg                                                                                                  <  1:8045062/151‑1 (MQ=255)
                                                   aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCt                                                                                                <  2:8125032/151‑1 (MQ=255)
                                                      gCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGTCGGTCCCGGCGCTGgc                                                                                             <  2:5548615/151‑1 (MQ=255)
                                                       ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                            <  1:5148367/151‑1 (MQ=255)
                                                       ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                            >  2:5722856/1‑151 (MQ=255)
                                                         aGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgc                                                                                          >  1:5780743/1‑151 (MQ=255)
                                                          ggCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgcc                                                                                         >  1:10006370/1‑151 (MQ=255)
                                                             gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTc                                                                                      <  2:4431560/151‑1 (MQ=255)
                                                             gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTc                                                                                      <  2:3216503/151‑1 (MQ=255)
                                                             gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTc                                                                                      <  2:7501322/151‑1 (MQ=255)
                                                               tGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCgg                                                                                    <  2:6064484/151‑1 (MQ=255)
                                                                 gAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTc                                                                                  <  2:10514422/151‑1 (MQ=255)
                                                                  aaGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCg                                                                                 <  2:4934672/151‑1 (MQ=255)
                                                                   aGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGc                                                                                <  2:6709245/151‑1 (MQ=255)
                                                                    gcgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGcc                                                                               <  1:842719/151‑1 (MQ=255)
                                                                       cAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTgg                                                                            >  1:2096731/1‑151 (MQ=255)
                                                                        aGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGc                                                                           >  1:6624781/1‑151 (MQ=255)
                                                                          ggCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGa                                                                         <  2:3514676/151‑1 (MQ=255)
                                                                           gCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGat                                                                        <  2:5106642/151‑1 (MQ=255)
                                                                            cATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatc                                                                       >  1:1184892/1‑151 (MQ=255)
                                                                                gccgcGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcg                                                                   >  1:716278/1‑151 (MQ=255)
                                                                                gccgcGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcg                                                                   >  1:5086243/1‑151 (MQ=255)
                                                                                  cgcgCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgcc                                                                 <  2:634690/151‑1 (MQ=255)
                                                                                   gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccg                                                                >  2:8251026/1‑151 (MQ=255)
                                                                                   gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccg                                                                <  1:522918/151‑1 (MQ=255)
                                                                                   gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCGGGCCGCCTTCGGTCGCCTGGCGATCATcgccg                                                                <  1:2539857/151‑1 (MQ=255)
                                                                                     gctgctgctGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCa                                                              <  1:5546563/151‑1 (MQ=255)
                                                                                       tgctgctgGGCGCGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCAcc                                                            <  1:10130410/151‑1 (MQ=255)
                                                                                        gctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCa                                                           <  2:7261091/151‑1 (MQ=255)
                                                                                          tgctgGGCGAGCCGGCCGAACTGGCGGCACTGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGc                                                         <  2:4789601/151‑1 (MQ=255)
                                                                                           gctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGcc                                                        >  1:370509/1‑151 (MQ=255)
                                                                                           gctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGcc                                                        <  1:8819759/151‑1 (MQ=255)
                                                                                           gctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGcc                                                        <  2:8716201/151‑1 (MQ=255)
                                                                                           gctgGGCGAGACGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGcc                                                        <  1:9101728/151‑1 (MQ=255)
                                                                                            ctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCg                                                       <  2:71772/151‑1 (MQ=255)
                                                                                               ggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACg                                                    <  2:278129/151‑1 (MQ=255)
                                                                                                      cggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                             >  1:9808940/1‑151 (MQ=255)
                                                                                                       ggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgtg                                            <  2:4174817/151‑1 (MQ=255)
                                                                                                        gccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgc                                           <  1:2775454/151‑1 (MQ=255)
                                                                                                          cgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCCTCGCCGCACCAGCCGACGGCCATGTgcgc                                         >  1:6750401/1‑151 (MQ=255)
                                                                                                          cgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcgc                                         <  2:7925726/151‑1 (MQ=255)
                                                                                                           gAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGcc                                        >  2:3053422/1‑151 (MQ=255)
                                                                                                            aaCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       >  1:7395714/1‑151 (MQ=255)
                                                                                                             aCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTg                                      <  1:9947734/151‑1 (MQ=255)
                                                                                                             aCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTAGGGCGCCTGGCGATCAACGCCGCACCAGCCGACGGCCATGTGCGccag                                      >  2:969400/1‑149 (MQ=255)
                                                                                                               tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGcc                                    >  2:6919088/1‑151 (MQ=255)
                                                                                                                 gcggcACAGGTGGAAGGGATCTTCCCCGGCAGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCgg                                  <  2:3727022/151‑1 (MQ=255)
                                                                                                                 gcggcACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCgg                                  <  2:8795339/151‑1 (MQ=255)
                                                                                                                     cacaGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGtt                              <  1:5622280/151‑1 (MQ=255)
                                                                                                                       caGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGa                            >  2:5830616/1‑151 (MQ=255)
                                                                                                                       caGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGa                            >  2:4538495/1‑151 (MQ=255)
                                                                                                                        aGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGac                           >  2:7421076/1‑150 (MQ=255)
                                                                                                                        aGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGaa                           <  1:10239478/151‑1 (MQ=255)
                                                                                                                          gTGGCAGGGATCTTCCCCGGCGGGGCCGAGGGGATCTTCGCTACGACCGGGTTCGGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcc                         <  2:6835269/151‑1 (MQ=255)
                                                                                                                            ggAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAGGTGCGCCTGCCGGCGTTGAACCTg                       >  1:3559336/1‑151 (MQ=255)
                                                                                                                                gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAcc                   <  2:1070167/151‑1 (MQ=255)
                                                                                                                                 ggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCg                  >  1:7601743/1‑151 (MQ=255)
                                                                                                                                 ggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCg                  >  2:3897685/1‑151 (MQ=255)
                                                                                                                                  gATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 >  1:7312963/1‑151 (MQ=255)
                                                                                                                                   aTCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTc                <  2:9006995/151‑1 (MQ=255)
                                                                                                                                    tCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcg               <  2:1608634/151‑1 (MQ=255)
                                                                                                                                    tCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcg               <  1:8693510/151‑1 (MQ=255)
                                                                                                                                      ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg             >  2:9267920/1‑151 (MQ=255)
                                                                                                                                       tCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCgg            <  2:1186614/151‑1 (MQ=255)
                                                                                                                                        ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt           >  1:9534571/1‑151 (MQ=255)
                                                                                                                                         cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg          >  1:182827/1‑151 (MQ=255)
                                                                                                                                         cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTGTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg          <  1:7775326/151‑1 (MQ=255)
                                                                                                                                            ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCt       >  1:6489221/1‑151 (MQ=255)
                                                                                                                                               ggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCgg    >  2:9482761/1‑151 (MQ=255)
                                                                                                                                                 ggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCggtg  >  2:2960733/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑