Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 480,880 T→C A204A (GCA→GCG)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463480,8800TC99.8% 1926.3 / NA 481A204A (GCA→GCG) PA14_RS0222016S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (241/239);  minor base A (0/1);  total (241/240)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

CACTGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCC  >  NC_008463/480732‑481027
                                                                                                                                                    |                                                                                                                                                   
 aCTGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGcc                                                                                                                                                  >  2:550421/1‑151 (MQ=255)
   tGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTg                                                                                                                                                <  1:10674121/151‑1 (MQ=255)
   tGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTg                                                                                                                                                >  1:8012890/1‑151 (MQ=255)
   tGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTg                                                                                                                                                >  1:2127790/1‑151 (MQ=255)
       tGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTcc                                                                                                                                            >  2:6911823/1‑151 (MQ=255)
       tGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTcc                                                                                                                                            <  1:1890139/151‑1 (MQ=255)
        gTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCa                                                                                                                                           >  1:1294973/1‑151 (MQ=255)
         tCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCAc                                                                                                                                          >  2:6642150/1‑151 (MQ=255)
          cGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCAcc                                                                                                                                         >  2:9697818/1‑151 (MQ=255)
          cGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCAcc                                                                                                                                         >  1:4641067/1‑151 (MQ=255)
           gCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCt                                                                                                                                        >  1:8234837/1‑151 (MQ=255)
            ccGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTc                                                                                                                                       <  1:7213054/151‑1 (MQ=255)
            ccGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTc                                                                                                                                       <  2:7925208/151‑1 (MQ=255)
               ttGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGc                                                                                                                                    >  1:1604206/1‑151 (MQ=255)
                tGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCa                                                                                                                                   >  1:5218275/1‑151 (MQ=255)
                 gTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCAt                                                                                                                                  <  1:2929617/151‑1 (MQ=255)
                 gTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCAt                                                                                                                                  >  2:3210332/1‑151 (MQ=255)
                   aaaGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCg                                                                                                                                >  2:2712551/1‑151 (MQ=255)
                      gcggcgGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTc                                                                                                                             >  1:7367844/1‑151 (MQ=255)
                       cggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCa                                                                                                                            <  2:5909199/151‑1 (MQ=255)
                        ggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAg                                                                                                                           >  2:1986246/1‑151 (MQ=255)
                        ggcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAg                                                                                                                           <  2:5267768/151‑1 (MQ=255)
                         gcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAgg                                                                                                                          <  1:8537721/151‑1 (MQ=255)
                         gcggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAgg                                                                                                                          <  2:8157135/151‑1 (MQ=255)
                          cggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgc                                                                                                                         >  1:6268428/1‑151 (MQ=255)
                          cggGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgc                                                                                                                         >  1:9710260/1‑151 (MQ=255)
                           gggAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgcc                                                                                                                        >  2:650518/1‑151 (MQ=255)
                           gggAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgcc                                                                                                                        <  2:124861/151‑1 (MQ=255)
                             gagagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgccgc                                                                                                                      >  2:8557985/1‑151 (MQ=255)
                             gagagaTATCAGGCGAAGTCGCCCCACAGTTGCTTGGACACCGACAACGATACCACCGCCGAGGTCTCGGTGCGCAGCAAACGCGGGCCGAGGCGCGCGGCGAGGAAGCCGGCCGACTACGCCAGCTCATCATCGGCATCGCTCAGcccgc                                                                                                                      >  1:10029408/1‑151 (MQ=255)
                              agagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGgccgcc                                                                                                                     <  2:9827240/151‑1 (MQ=255)
                               gagaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCt                                                                                                                    <  1:2813513/151‑1 (MQ=255)
                                 gaTATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCttc                                                                                                                  >  1:5013285/1‑150 (MQ=255)
                                  atatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCttcg                                                                                                                 >  1:3285948/1‑149 (MQ=255)
                                  atatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCttcg                                                                                                                 <  2:6186507/151‑3 (MQ=255)
                                   tatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCgg                                                                                                                <  1:5726078/151‑1 (MQ=255)
                                    atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                               >  1:2487591/1‑151 (MQ=255)
                                       aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                            <  2:5107647/151‑1 (MQ=255)
                                       aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                            >  2:8949388/1‑151 (MQ=255)
                                             aGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAg                                                                                                      >  2:156523/1‑151 (MQ=255)
                                                  ccccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAgg                                                                                                 >  2:38530/1‑151 (MQ=255)
                                                   cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                <  1:3211515/151‑1 (MQ=255)
                                                   cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                >  1:8121511/1‑151 (MQ=255)
                                                    ccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGcc                                                                                               <  1:6101955/151‑1 (MQ=255)
                                                        agTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgc                                                                                           >  1:4045689/1‑151 (MQ=255)
                                                          ttGCTGGGCCACCGCCAACGCGCCCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcgc                                                                                         <  2:4493161/151‑1 (MQ=255)
                                                           tGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGcgcg                                                                                        >  1:6589868/1‑151 (MQ=255)
                                                              tGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcg                                                                                     <  1:8657420/151‑1 (MQ=255)
                                                                ggCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGACGGCCAGGCGCGAcggc                                                                                   >  2:8422097/1‑151 (MQ=255)
                                                                ggCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggc                                                                                   <  1:9627622/151‑1 (MQ=255)
                                                                        ccAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtg                                                                           <  1:4496698/151‑1 (MQ=255)
                                                                         cAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtgg                                                                          <  2:4359708/151‑1 (MQ=255)
                                                                           aCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGGGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                        >  2:10257193/1‑151 (MQ=255)
                                                                            cGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctg                                                                       <  1:1778559/151‑1 (MQ=255)
                                                                             gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctgg                                                                      <  1:3613584/151‑1 (MQ=255)
                                                                              cTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctggc                                                                     <  1:7319820/151‑1 (MQ=255)
                                                                                  caccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgg                                                                 >  2:8707444/1‑151 (MQ=255)
                                                                                       gcgcGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggc                                                            <  1:10546442/151‑1 (MQ=255)
                                                                                       gcgcGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggc                                                            >  2:9183074/1‑151 (MQ=255)
                                                                                         gcgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcgg                                                          >  2:2329776/1‑151 (MQ=255)
                                                                                          cgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcggc                                                         >  1:9389593/1‑151 (MQ=255)
                                                                                            gTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGA‑CCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcag                                                      <  2:4221450/151‑2 (MQ=255)
                                                                                              ctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcaca                                                     <  2:6338304/151‑1 (MQ=255)
                                                                                              ctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcaca                                                     <  2:3324774/151‑1 (MQ=255)
                                                                                               tcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAg                                                    >  2:7517976/1‑151 (MQ=255)
                                                                                                cGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAgg                                                   >  2:10607501/1‑151 (MQ=255)
                                                                                                  gTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGt                                                 <  2:938758/151‑1 (MQ=255)
                                                                                                     cgcAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCa                                              <  2:10044593/151‑1 (MQ=255)
                                                                                                        agcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGa                                           <  2:5813271/151‑1 (MQ=255)
                                                                                                         gcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGAc                                          <  2:2221140/151‑1 (MQ=255)
                                                                                                           aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                        >  1:7778965/1‑151 (MQ=255)
                                                                                                            cTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAg                                       <  2:4108702/151‑1 (MQ=255)
                                                                                                             tCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGc                                      <  2:5706051/151‑1 (MQ=255)
                                                                                                                 gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                  >  1:4240375/1‑151 (MQ=255)
                                                                                                                 gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCGTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                  <  2:4816547/151‑1 (MQ=255)
                                                                                                                     cGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTc                              <  1:5432933/151‑1 (MQ=255)
                                                                                                                     cGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTc                              >  1:4817550/1‑151 (MQ=255)
                                                                                                                      gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                             <  2:4992739/151‑1 (MQ=255)
                                                                                                                         gcgcgcGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCg                          <  1:10322988/151‑1 (MQ=255)
                                                                                                                           gcgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTc                        >  1:3131108/1‑151 (MQ=255)
                                                                                                                           gcgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTc                        <  1:7678706/151‑1 (MQ=255)
                                                                                                                            cgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                       <  1:8949388/151‑1 (MQ=255)
                                                                                                                            cgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                       <  2:7192930/151‑1 (MQ=255)
                                                                                                                            cgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                       <  2:1685562/151‑1 (MQ=255)
                                                                                                                              cggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccg                     <  1:69234/151‑1 (MQ=255)
                                                                                                                              cggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccg                     <  1:601215/151‑1 (MQ=255)
                                                                                                                                gcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgcc                   >  2:1845926/1‑151 (MQ=255)
                                                                                                                                gcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgcc                   >  1:1225336/1‑151 (MQ=255)
                                                                                                                                 cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                  <  1:4388679/151‑1 (MQ=255)
                                                                                                                                  gTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgcccg                 <  2:4054484/151‑1 (MQ=255)
                                                                                                                                   tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                <  2:7338651/151‑1 (MQ=255)
                                                                                                                                   tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                >  1:9189236/1‑151 (MQ=255)
                                                                                                                                    ggAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCcgcg               >  1:9214382/1‑151 (MQ=255)
                                                                                                                                        gccggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGc           <  1:2232896/151‑1 (MQ=255)
                                                                                                                                         ccggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGcc          <  1:5601083/151‑1 (MQ=255)
                                                                                                                                         ccggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGcc          >  1:7529681/1‑151 (MQ=255)
                                                                                                                                            gccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGt       >  2:9523204/1‑151 (MQ=255)
                                                                                                                                             ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtc      <  2:7023010/151‑1 (MQ=255)
                                                                                                                                               gccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcct    >  2:365058/1‑151 (MQ=255)
                                                                                                                                               gccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcct    >  1:1018855/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACTGGCTGTCGCCGTTGTAAAGCGGCGGGAGAGATATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCC  >  NC_008463/480732‑481027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑