Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 729,022 C→T G232D (GGC→GAC)  PA14_RS03435 ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463729,0220CT100.0% 2424.9 / NA 560G232D (GGC→GAC) PA14_RS03435anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (294/266);  total (294/266)

Only 100 of 560 total aligned reads displayed.

CTGGGCGTCCAGCAGCGATTCGCTGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGCCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCGGGCCGC  >  NC_008463/728874‑729169
                                                                                                                                                    |                                                                                                                                                   
 tGGGCGTCCAGCAGCGATTCGCTGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATCTCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCAc                                                                                                                                                  >  1:7614918/1‑151 (MQ=255)
    gCGTCCAGCAGCGATTCGCTGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCAcgcg                                                                                                                                               <  1:1589467/151‑1 (MQ=255)
                    cGCTGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATc                                                                                                                               <  1:4317091/151‑1 (MQ=255)
                       tGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGc                                                                                                                            >  1:1258986/1‑151 (MQ=255)
                       tGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGc                                                                                                                            >  2:9259476/1‑151 (MQ=255)
                       tGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGc                                                                                                                            >  1:3426695/1‑151 (MQ=255)
                        gATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGcc                                                                                                                           >  1:5893497/1‑151 (MQ=255)
                           gctgcGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCcagc                                                                                                                        <  2:2736019/151‑1 (MQ=255)
                              gcgcgcgcTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAgg                                                                                                                     >  1:9565132/1‑151 (MQ=255)
                                gcgcgcTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCt                                                                                                                   >  1:8954897/1‑151 (MQ=255)
                                 cgcgcTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGACCTCATCCGCCAGCAGGCtt                                                                                                                  >  2:9457039/1‑151 (MQ=255)
                                    gcTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGcc                                                                                                               >  1:7344790/1‑151 (MQ=255)
                                     cTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCca                                                                                                              >  2:34304/1‑151 (MQ=255)
                                      tCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCcag                                                                                                             >  1:2413386/1‑151 (MQ=255)
                                       cAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCcagc                                                                                                            <  1:10717956/151‑1 (MQ=255)
                                        aGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCcagca                                                                                                           >  1:1419789/1‑151 (MQ=255)
                                         gCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCcagcag                                                                                                          >  2:5975408/1‑151 (MQ=255)
                                           tCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTAGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTg                                                                                                        <  2:4771539/151‑1 (MQ=255)
                                            ccAGCAGGGTTGCCTGGATAACCGCAGCCGGCAAGGCAGGGTGACGAGCCAGGAGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTAGCCAGCAGTGc                                                                                                       >  1:7143438/1‑151 (MQ=255)
                                             cagcagGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCg                                                                                                      <  1:8192354/151‑1 (MQ=255)
                                              agcagGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACCGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGt                                                                                                     >  1:4064871/1‑151 (MQ=255)
                                                cagGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTgg                                                                                                   <  1:9801489/151‑1 (MQ=255)
                                                 agGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTGGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGt                                                                                                  >  2:5228915/1‑151 (MQ=255)
                                                  gggTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGtt                                                                                                 >  1:4925911/1‑151 (MQ=255)
                                                    gTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCa                                                                                               <  1:9024468/151‑1 (MQ=255)
                                                    gTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCa                                                                                               <  1:9335691/151‑1 (MQ=255)
                                                     ttGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCAt                                                                                              <  1:174465/151‑1 (MQ=255)
                                                       gCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCt                                                                                            <  1:4688769/151‑1 (MQ=255)
                                                       gCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCt                                                                                            >  2:661836/1‑151 (MQ=255)
                                                          tGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGc                                                                                         >  1:8780620/1‑151 (MQ=255)
                                                          tGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGc                                                                                         >  2:10296798/1‑151 (MQ=255)
                                                          tGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGc                                                                                         >  1:7704032/1‑151 (MQ=255)
                                                          tGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGc                                                                                         <  2:1328980/151‑1 (MQ=255)
                                                          tGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGc                                                                                         >  2:4139705/1‑151 (MQ=255)
                                                            gATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCg                                                                                       <  1:2536885/151‑1 (MQ=255)
                                                               atCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTg                                                                                    <  2:6091996/151‑1 (MQ=255)
                                                               atCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTg                                                                                    <  1:1912003/151‑1 (MQ=255)
                                                                tCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTgg                                                                                   >  2:2087784/1‑151 (MQ=255)
                                                                   gCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTggcgg                                                                                <  1:6622397/151‑1 (MQ=255)
                                                                    cTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTggcggc                                                                               <  2:10627125/151‑1 (MQ=255)
                                                                    cTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTggcggc                                                                               >  1:2088460/1‑151 (MQ=255)
                                                                          gCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGc                                                                         >  2:2379657/1‑151 (MQ=255)
                                                                           cAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTGCATCTGGCCGCTGGCGGCCCACGCa                                                                        >  2:1221731/1‑151 (MQ=255)
                                                                            aaGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCAc                                                                       >  2:7988332/1‑151 (MQ=255)
                                                                            aaGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCAc                                                                       >  1:6318161/1‑151 (MQ=255)
                                                                            aaGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCAc                                                                       >  1:7747347/1‑151 (MQ=255)
                                                                            aaGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGGGGTTCATCTGGCCGCTGGCGGCCCACGCAc                                                                       >  1:3854810/1‑151 (MQ=255)
                                                                             aGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCAcc                                                                      >  2:726379/1‑151 (MQ=255)
                                                                              ggCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCg                                                                     <  1:1006444/151‑1 (MQ=255)
                                                                                  gggTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTccc                                                                 >  2:10001532/1‑151 (MQ=255)
                                                                                  gggTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTccc                                                                 >  1:1039315/1‑151 (MQ=255)
                                                                                  gggTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTccc                                                                 <  2:8739884/151‑1 (MQ=255)
                                                                                     tGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGt                                                              >  2:1548575/1‑151 (MQ=255)
                                                                                      gACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTc                                                             >  2:6972753/1‑151 (MQ=255)
                                                                                      gACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTc                                                             >  1:5145275/1‑151 (MQ=255)
                                                                                         gAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGaa                                                          >  2:8384208/1‑151 (MQ=255)
                                                                                          aGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGaaa                                                         <  2:4967278/151‑1 (MQ=255)
                                                                                          aGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGaaa                                                         >  2:7431773/1‑151 (MQ=255)
                                                                                             cAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGt                                                      >  2:8872012/1‑151 (MQ=255)
                                                                                              aGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGtt                                                     <  2:9561332/151‑1 (MQ=255)
                                                                                              aGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGtt                                                     <  2:2514196/151‑1 (MQ=255)
                                                                                               ggTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTc                                                    >  2:3260749/1‑151 (MQ=255)
                                                                                                 tGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTcgc                                                  >  2:6703585/1‑151 (MQ=255)
                                                                                                   cTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTcgccg                                                <  1:9606886/151‑1 (MQ=255)
                                                                                                   cTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTcgccg                                                >  2:8664462/1‑151 (MQ=255)
                                                                                                    tCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTcgccgc                                               <  1:3151253/151‑1 (MQ=255)
                                                                                                       tGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGtt                                            >  1:6097278/1‑151 (MQ=255)
                                                                                                          aGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGt                                         <  2:8980178/151‑1 (MQ=255)
                                                                                                            ccAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGtga                                       >  2:7870609/1‑151 (MQ=255)
                                                                                                            ccAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGtga                                       <  1:10606481/151‑1 (MQ=255)
                                                                                                            ccAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGtga                                       <  2:6319058/151‑1 (MQ=255)
                                                                                                             cAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGtgat                                      <  2:577376/151‑1 (MQ=255)
                                                                                                              aGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGtgatg                                     <  2:1897254/151‑1 (MQ=255)
                                                                                                                gACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGaa                                   <  2:5346881/151‑1 (MQ=255)
                                                                                                                 aCAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAAt                                  >  2:3534839/1‑151 (MQ=255)
                                                                                                                 aCAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAAt                                  >  1:2277781/1‑151 (MQ=255)
                                                                                                                  cAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATc                                 >  1:3258591/1‑151 (MQ=255)
                                                                                                                  cAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATc                                 <  1:8037653/151‑1 (MQ=255)
                                                                                                                   aGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATcc                                >  1:2704237/1‑151 (MQ=255)
                                                                                                                        gAAGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCg                           >  1:6864821/1‑151 (MQ=255)
                                                                                                                         aaGCGCTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGt                          >  2:3837102/1‑151 (MQ=255)
                                                                                                                            cgcTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCa                       >  1:5454336/1‑151 (MQ=255)
                                                                                                                             gcTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCAt                      <  2:5711605/151‑1 (MQ=255)
                                                                                                                             gcTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCAt                      <  1:5736841/151‑1 (MQ=255)
                                                                                                                              cTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATc                     <  1:8560/151‑1 (MQ=255)
                                                                                                                              cTCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATc                     <  1:8391967/151‑1 (MQ=255)
                                                                                                                               tCGCGCCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCa                    <  1:2920328/151‑1 (MQ=255)
                                                                                                                                 gcgcCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGc                  <  2:5813853/151‑1 (MQ=255)
                                                                                                                                 gcgcCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGc                  >  1:6231321/1‑151 (MQ=255)
                                                                                                                                  cgcCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCa                 <  1:945651/151‑1 (MQ=255)
                                                                                                                                  cgcCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCa                 <  1:5373941/151‑1 (MQ=255)
                                                                                                                                   gcCCGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCAc                >  2:4533385/1‑151 (MQ=255)
                                                                                                                                      cGGTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGtt             <  2:4067231/151‑1 (MQ=255)
                                                                                                                                       ggTGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTg            <  1:3701061/151‑1 (MQ=255)
                                                                                                                                         tGCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGcc          <  2:975598/151‑1 (MQ=255)
                                                                                                                                          gCTCTTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGccc         >  1:6786093/1‑151 (MQ=255)
                                                                                                                                           ctctTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCg        >  1:1833678/1‑151 (MQ=255)
                                                                                                                                            tctTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCgg       <  2:3024118/151‑1 (MQ=255)
                                                                                                                                             ctTGGGGTCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCggg      <  2:8048431/151‑1 (MQ=255)
                                                                                                                                               tGGGGTCACGCGCGGCGAAGAACACATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCGGGcc    <  1:4409228/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGGGCGTCCAGCAGCGATTCGCTGATGCTGCGCGCGCTCAGCTCCAGCAGGGTTGCCTGGATATCCGCTGCCGGCAAGGCAGGGTGACGAGCCAGGTGCTCCTGCAGCCAGGACAGGTTGAAGCGCTCGCGCCCGGTGCTCTTGGGGCCACGCGCGGCGAAGAACTCATCCGCCAGCAGGCTTGCCAGCAGTGCGTGGTTCATCTGGCCGCTGGCGGCCCACGCACCGTCCCGGTCGAAATGTTCGCCGCGTTGGTGATGAATCCAGGCGTCCATCAGCACGTTGCCCGGGCCGC  >  NC_008463/728874‑729169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑