Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,905,079 A→G F731F (TTT→TTC)  glnE ← bifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,905,0790AG99.8% 1795.4 / NA 457F731F (TTT→TTC) glnEbifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (246/210);  minor base C (1/0);  total (247/210)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 458 total aligned reads displayed.

GCAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATAAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGTTCG  >  NC_008463/5904931‑5905226
                                                                                                                                                    |                                                                                                                                                   
 cAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGCTGAac                                                                                                                                                  >  2:1926352/1‑151 (MQ=255)
 cAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAac                                                                                                                                                  >  1:6081189/1‑151 (MQ=255)
   ggCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAacac                                                                                                                                                >  1:5236785/1‑151 (MQ=255)
     cgcATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCa                                                                                                                                              >  2:835193/1‑151 (MQ=255)
         tGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTc                                                                                                                                          <  1:4182985/151‑1 (MQ=255)
              aCCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGt                                                                                                                                     >  1:8209163/1‑151 (MQ=255)
                cTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTcc                                                                                                                                   <  1:9681329/151‑1 (MQ=255)
                cTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCGTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTcc                                                                                                                                   <  2:5751110/151‑1 (MQ=255)
                  cGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGa                                                                                                                                 >  2:2154079/1‑151 (MQ=255)
                  cGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGa                                                                                                                                 <  2:1572504/151‑1 (MQ=255)
                   gTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAg                                                                                                                                <  1:5122776/151‑1 (MQ=255)
                   gTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAg                                                                                                                                <  2:10722213/151‑1 (MQ=255)
                       agGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCAt                                                                                                                            <  2:1732894/151‑1 (MQ=255)
                         ggTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGACCACCAGGTCGAGGTCCGAGCCATGa                                                                                                                          <  2:2690018/151‑1 (MQ=255)
                           tGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGAcc                                                                                                                        <  1:9314227/151‑1 (MQ=255)
                            gCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGACATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCg                                                                                                                       >  1:782319/1‑151 (MQ=255)
                                      gTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGcc                                                                                                             >  1:499403/1‑151 (MQ=255)
                                       tCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGccc                                                                                                            <  2:7874650/151‑1 (MQ=255)
                                        cTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCccg                                                                                                           <  1:8367828/151‑1 (MQ=255)
                                         tGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCccgc                                                                                                          >  1:8811111/1‑151 (MQ=255)
                                          gcgcGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCccgcc                                                                                                         >  2:10401739/1‑151 (MQ=255)
                                             cgGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGAc                                                                                                      >  1:5583454/1‑151 (MQ=255)
                                              ggTGAGGAAATGGATGAGCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGGCCGAGCCATGACCGAACTCCAGCCCGCCGAcc                                                                                                     >  2:1211076/1‑151 (MQ=255)
                                               gTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCt                                                                                                    >  1:4432891/1‑151 (MQ=255)
                                                tGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCtt                                                                                                   <  1:9813094/151‑1 (MQ=255)
                                                tGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCtt                                                                                                   <  2:9402885/151‑1 (MQ=255)
                                                 gAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTg                                                                                                  <  1:2490691/151‑1 (MQ=255)
                                                  aGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGc                                                                                                 >  2:5470894/1‑151 (MQ=255)
                                                     aaaTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGt                                                                                              >  2:7019853/1‑151 (MQ=255)
                                                     aaaTGGATGATCGTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGt                                                                                              <  2:4626898/151‑1 (MQ=255)
                                                      aaTGGATGATCTTCTGCCCCAGGCGGGTAAAGATCTGCGCGCCCTCGATGGATTTGCCGCCATCGGTCTGGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGACCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTa                                                                                             <  2:4438752/151‑1 (MQ=255)
                                                      aaTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTa                                                                                             >  2:7324224/1‑151 (MQ=255)
                                                      aaTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTa                                                                                             >  1:8013097/1‑151 (MQ=255)
                                                      aaTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTa                                                                                             <  1:1786330/151‑1 (MQ=255)
                                                       aTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAg                                                                                            >  1:2718448/1‑151 (MQ=255)
                                                       aTGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAg                                                                                            >  1:4359068/1‑151 (MQ=255)
                                                         ggATGATCTTCTGCCCCAGGGGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGACCACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGcc                                                                                          <  2:6496876/151‑1 (MQ=255)
                                                         ggATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGcc                                                                                          <  1:3505260/151‑1 (MQ=255)
                                                           atgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcga                                                                                        >  1:160370/1‑151 (MQ=255)
                                                           atgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcga                                                                                        >  1:5698299/1‑151 (MQ=255)
                                                           atgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATGTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcga                                                                                        <  2:6534902/151‑1 (MQ=255)
                                                            tgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcgac                                                                                       <  2:6668331/151‑1 (MQ=255)
                                                              atCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcgacga                                                                                     >  1:3350734/1‑151 (MQ=255)
                                                               tcttctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGAt                                                                                    <  2:4027017/151‑1 (MQ=255)
                                                                cttctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATg                                                                                   >  2:5158360/1‑151 (MQ=255)
                                                                   ctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACg                                                                                <  2:5260581/151‑1 (MQ=255)
                                                                   ctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACg                                                                                >  1:1125897/1‑151 (MQ=255)
                                                                     gCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGaa                                                                              >  1:10319674/1‑151 (MQ=255)
                                                                       cccAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGt                                                                            >  2:3363857/1‑151 (MQ=255)
                                                                       cccAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGt                                                                            >  2:3632017/1‑151 (MQ=255)
                                                                        ccAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTc                                                                           >  1:7945895/1‑151 (MQ=255)
                                                                          aGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCg                                                                         >  1:10642359/1‑151 (MQ=255)
                                                                           ggCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCgg                                                                        >  2:6872628/1‑151 (MQ=255)
                                                                             cGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGAt                                                                      <  2:2673359/151‑1 (MQ=255)
                                                                               ggTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCg                                                                    >  1:6761399/1‑151 (MQ=255)
                                                                                gTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCTAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGc                                                                   <  2:4776284/151‑1 (MQ=255)
                                                                                  aaaGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCAc                                                                 >  1:4629330/1‑151 (MQ=255)
                                                                                   aaGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGACCTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACg                                                                <  2:8984630/151‑1 (MQ=255)
                                                                                    aGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                               >  2:10559840/1‑151 (MQ=255)
                                                                                       aCTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGt                                                            <  1:6033412/151‑1 (MQ=255)
                                                                                       aCTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGt                                                            >  2:6106429/1‑151 (MQ=255)
                                                                                        cTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTg                                                           >  2:5553373/1‑151 (MQ=255)
                                                                                              gcCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCg                                                     <  2:1523976/151‑1 (MQ=255)
                                                                                                 aTCGATGGATTTGCCGCCATCGGTATCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcg                                                  >  1:8588010/1‑151 (MQ=255)
                                                                                                  tCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgc                                                 >  1:7052080/1‑151 (MQ=255)
                                                                                                   cGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcg                                                >  2:8575447/1‑151 (MQ=255)
                                                                                                   cGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcg                                                >  1:2789342/1‑151 (MQ=255)
                                                                                                    gATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcgc                                               >  2:2513899/1‑151 (MQ=255)
                                                                                                    gATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcgc                                               <  1:4842569/151‑1 (MQ=255)
                                                                                                    gATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcgc                                               >  1:1424921/1‑151 (MQ=255)
                                                                                                    gATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcgc                                               >  1:3162611/1‑151 (MQ=255)
                                                                                                       ggATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAAc                                            >  1:8774467/1‑151 (MQ=255)
                                                                                                                  cATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTg                                 >  2:1343706/1‑151 (MQ=255)
                                                                                                                  cATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTg                                 <  1:5834534/151‑1 (MQ=255)
                                                                                                                  cATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTg                                 >  2:10128989/1‑151 (MQ=255)
                                                                                                                    tCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGAc                               <  1:7420404/151‑1 (MQ=255)
                                                                                                                     cGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACg                              >  1:540868/1‑151 (MQ=255)
                                                                                                                        tctcGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTg                           >  2:9262466/1‑151 (MQ=255)
                                                                                                                        tctcGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTg                           >  2:10178039/1‑151 (MQ=255)
                                                                                                                         ctcGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGc                          <  2:7550535/151‑1 (MQ=255)
                                                                                                                           cGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCAc                        <  1:3642826/151‑1 (MQ=255)
                                                                                                                             cAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCa                      <  2:7932033/151‑1 (MQ=255)
                                                                                                                              aGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCaa                     >  1:10022613/1‑151 (MQ=255)
                                                                                                                                 gggggTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTg                  >  1:7311376/1‑151 (MQ=255)
                                                                                                                                   gggTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTgcc                <  2:3280545/151‑1 (MQ=255)
                                                                                                                                       cGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGACCTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgcca            <  2:3044820/151‑1 (MQ=255)
                                                                                                                                       cGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgcca            <  2:2253362/151‑1 (MQ=255)
                                                                                                                                        gCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccag           >  1:1472281/1‑151 (MQ=255)
                                                                                                                                        gCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccag           >  2:8708863/1‑151 (MQ=255)
                                                                                                                                          cGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgcccggc         >  2:6041527/1‑151 (MQ=255)
                                                                                                                                          cGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggc         >  1:9545394/1‑151 (MQ=255)
                                                                                                                                           gTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggcc        >  1:6650457/1‑151 (MQ=255)
                                                                                                                                            tCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggcca       >  1:3935060/1‑151 (MQ=255)
                                                                                                                                             cATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggccag      >  2:3778292/1‑151 (MQ=255)
                                                                                                                                             cATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggccag      >  1:2152526/1‑151 (MQ=255)
                                                                                                                                              aTGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGt     <  1:7663061/151‑1 (MQ=255)
                                                                                                                                              aTGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGt     >  2:3531027/1‑151 (MQ=255)
                                                                                                                                               tGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGtt    >  1:184667/1‑151 (MQ=255)
                                                                                                                                                 gATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGTTCg  <  1:9008549/151‑1 (MQ=255)
                                                                                                                                                 gATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGTTCg  >  1:62481/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATAAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGTTCG  >  NC_008463/5904931‑5905226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑